ATCC Wolfe's mineral mix

Ontology mapping

Primary identifier: kgmicrobe.ingredient:atcc_wolfes_mineral_mix
Notes: Imported from CultureBotHT (consolidated_media.json (media-only)). Used in 46 CultureBot media; samples: NLDM_defined_nocarbon_novitamin, Mega, NLDM_defined_no_iron…. No CAS-RN or CHEBI mapping available; reviewed on 2026-05-11 as an ATCC-branded Wolfe mineral stock/pre-mix pending component-level recipe curation.

Nutritional roles

Role
MINERAL_SOURCE
Confidence
1.0
Evidence
- Reference text
  ATCC Medium 2672 Modified Wolfe's Mineral Media
  Reference type
  TECHNICAL_REPORT
  Url
  https://www.atcc.org/~/media/a457a0ae8c604a12b5597e5eb1a6583a.ashx
  Excerpt
  Trace Mineral Supplement, catalog no. MD-TMS
  Curator note
  ATCC Medium 2672 enumerates the ready-to-use ATCC MD-TMS stock as a Wolfe's mineral solution containing bulk inorganic mineral salts.
Role
TRACE_ELEMENT
Confidence
1.0
Evidence
- Reference text
  ATCC Medium 2672 Modified Wolfe's Mineral Media
  Reference type
  TECHNICAL_REPORT
  Url
  https://www.atcc.org/~/media/a457a0ae8c604a12b5597e5eb1a6583a.ashx
  Excerpt
  Trace Mineral Supplement, catalog no. MD-TMS
  Curator note
  ATCC Medium 2672 enumerates the ready-to-use ATCC MD-TMS stock as a Wolfe's mineral solution containing iron, cobalt, zinc, copper, aluminium, boron, and molybdate trace salts.

Synonyms (1)

Synonym	Type	Source	Occurrences
ATCC Wolfe's mineral mix	RAW_TEXT	culturebotht

Evidence (1)

DATABASE_MATCH via MIM curation (#114)

ATCC Wolfe's mineral mix is a named, recurring multi-component preparation (46 media). Searched CHEBI, NCIT, MeSH, FOODON and ENVO by label and synonym with normalisation: no term denotes it, and none could -- it is a lab preparation rather than a substance. Takes the STOCK_SOLUTION convention used by the existing mapped records: a kgmicrobe.ingredient: mint, with no parent because a multi-component mix does not narrow to any single compound (#114).

Material components

Method: RECIPE_TRANSCRIPTION
Completeness: COMPLETE

Component	Identifier	Scope	Concentration
EDTA	`CHEBI:4735`	MIM_CATALOG	0.5 G_PER_L
MgSO4.7H2O	`CHEBI:31795`	MIM_CATALOG	3.0 G_PER_L
MnSO4.H2O	`CHEBI:86364`	MIM_CATALOG	0.5 G_PER_L
NaCl	`CHEBI:26710`	MIM_CATALOG	1.0 G_PER_L
FeSO4.7H2O	`CHEBI:75836`	MIM_CATALOG	0.1 G_PER_L
CoCl2 x 6 H2O	`CHEBI:53503`	MIM_CATALOG	0.1 G_PER_L
CaCl2	`CHEBI:3312`	MIM_CATALOG	0.1 G_PER_L
ZnSO4.7H2O	`CHEBI:32312`	MIM_CATALOG	0.1 G_PER_L
CuSO4.5H2O	`CHEBI:31440`	MIM_CATALOG	0.01 G_PER_L
AlK(SO4)2.12H2O	`CHEBI:86465`	MIM_CATALOG	0.01 G_PER_L
H3BO3	`CHEBI:33118`	MIM_CATALOG	0.01 G_PER_L
Na2MoO4.2H2O	`CHEBI:75213`	MIM_CATALOG	0.01 G_PER_L
Distilled water	`CHEBI:15377`	MIM_CATALOG	1.0 L_PER_L

Decomposition evidence

RECIPE_SOURCE https://www.atcc.org/~/media/a457a0ae8c604a12b5597e5eb1a6583a.ashx — ATCC Medium 2672 Wolfe's Mineral Solution

ATCC Medium 2672 includes the MD-TMS Wolfe's Mineral Solution formulation and per-liter amounts.

Occurrence statistics

Total occurrences: 46
Distinct media: 46

Curation history

Timestamp	Curator	Action	New status	Changes
2026-04-29T06:51:33.117170+00:00	audit_add_remaining_culturebotht	CREATED_AS_UNMAPPED	UNMAPPED	Imported from CultureBotHT (consolidated_media.json (media-only)). Used in 46 CultureBot media; samples: NLDM_defined_nocarbon_novitamin, Mega, NLDM_defined_no_iron…. No CAS-RN or CHEBI mapping available; curator review needed.
2026-05-11T08:35:00Z	mediaingredientmech-agentic-curation LLM	CLASSIFIED_STOCK_SOLUTION	UNMAPPED	Classified as an ATCC-branded Wolfe mineral stock/pre-mix and left unmapped pending recipe/component curation.
2026-08-06T00:00:00+00:00	promote_resolved_unmapped	PROMOTED_TO_MAPPED	MAPPED	Promoted UNMAPPED_0298 -> kgmicrobe.ingredient:atcc_wolfes_mineral_mix "ATCC Wolfe's mineral mix" (FALLBACK_REGISTRY); mapping_status UNMAPPED -> MAPPED, SSSOM row added.
2026-09-04T00:00:00+00:00	mediaingredientmech-agentic-curation LLM	ANNOTATED		Added nutritional mineral/trace-element roles plus components and component_assertion by transcribing the ATCC MD-TMS Wolfe's Mineral Solution formula in ATCC Medium 2672.