ATCC Wolfe's vitamin mix

kgmicrobe.ingredient:atcc_wolfes_vitamin_mix defined mix MAPPED kgmicrobe.ingredient FALLBACK_REGISTRY

Ontology mapping

Primary identifier
kgmicrobe.ingredient:atcc_wolfes_vitamin_mix
Notes
Imported from CultureBotHT (consolidated_media.json (media-only)). Used in 43 CultureBot media; samples: Mega, NLDM_defined, M9 minimal media plus…. No CAS-RN or CHEBI mapping available; reviewed on 2026-05-11 as an ATCC-branded Wolfe vitamin stock/pre-mix pending component-level recipe curation.

Nutritional roles

Synonyms (1)

SynonymTypeSourceOccurrences
ATCC Wolfe's vitamin mix RAW_TEXT culturebotht

Evidence (1)

DATABASE_MATCH via MIM curation (#114)

ATCC Wolfe's vitamin mix is a named, recurring multi-component preparation (43 media). Searched CHEBI, NCIT, MeSH, FOODON and ENVO by label and synonym with normalisation: no term denotes it, and none could -- it is a lab preparation rather than a substance. Takes the STOCK_SOLUTION convention used by the existing mapped records: a kgmicrobe.ingredient: mint, with no parent because a multi-component mix does not narrow to any single compound (#114).

Material components

Method
RECIPE_TRANSCRIPTION
Completeness
COMPLETE
ComponentIdentifierScopeConcentration
Biotin CHEBI:15956 MIM_CATALOG 2.0 MG_PER_L
Folic acid CHEBI:27470 MIM_CATALOG 2.0 MG_PER_L
Pyridoxine hydrochloride CHEBI:30961 MIM_CATALOG 10.0 MG_PER_L
Thiamine HCl CHEBI:49105 MIM_CATALOG 5.0 MG_PER_L
Riboflavin CHEBI:17015 MIM_CATALOG 5.0 MG_PER_L
Nicotinic acid CHEBI:15940 MIM_CATALOG 5.0 MG_PER_L
Calcium D-(+)-pantothenate CHEBI:31345 MIM_CATALOG 5.0 MG_PER_L
Vitamin B12 CHEBI:176843 MIM_CATALOG 0.1 MG_PER_L
p-Aminobenzoic acid CHEBI:30753 MIM_CATALOG 5.0 MG_PER_L
Thioctic acid CHEBI:16494 MIM_CATALOG 5.0 MG_PER_L
Distilled water CHEBI:15377 MIM_CATALOG 1.0 L_PER_L

Decomposition evidence

RECIPE_SOURCE https://www.atcc.org/~/media/a457a0ae8c604a12b5597e5eb1a6583a.ashx — ATCC Medium 2672 Wolfe's Vitamin Solution

ATCC Medium 2672 includes the MD-VS Wolfe's Vitamin Solution formulation and per-liter amounts.

Occurrence statistics

Total occurrences
43
Distinct media
43

Curation history

TimestampCuratorActionNew statusChanges
2026-04-29T06:51:33.117170+00:00 audit_add_remaining_culturebotht CREATED_AS_UNMAPPED UNMAPPED Imported from CultureBotHT (consolidated_media.json (media-only)). Used in 43 CultureBot media; samples: Mega, NLDM_defined, M9 minimal media plus…. No CAS-RN or CHEBI mapping available; curator review needed.
2026-05-11T08:35:00Z mediaingredientmech-agentic-curation LLM CLASSIFIED_STOCK_SOLUTION UNMAPPED Classified as an ATCC-branded Wolfe vitamin stock/pre-mix and left unmapped pending recipe/component curation.
2026-08-06T00:00:00+00:00 promote_resolved_unmapped PROMOTED_TO_MAPPED MAPPED Promoted UNMAPPED_0259 -> kgmicrobe.ingredient:atcc_wolfes_vitamin_mix "ATCC Wolfe's vitamin mix" (FALLBACK_REGISTRY); mapping_status UNMAPPED -> MAPPED, SSSOM row added.
2026-09-04T00:00:00+00:00 mediaingredientmech-agentic-curation LLM ANNOTATED Added nutritional_roles.VITAMIN_SOURCE plus components and component_assertion by transcribing the ATCC MD-VS Wolfe's Vitamin Solution formula in ATCC Medium 2672.
2026-09-11T14:49:05.979520+00:00 fix_lipoic_stereochemistry CORRECTED Retargeted Thioctic acid component CHEBI:30314 -> CHEBI:16494 after the source label moved from the R-enantiomer to generic lipoic acid (#454).