HEPES

CHEBI:42334 chemically defined MAPPED CHEBI EXACT_MATCH

Ontology mapping

Primary identifier
CHEBI:42334
Ontology label
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
kg-microbe node
CHEBI:42334

Physicochemical roles

Synonyms (13)

SynonymTypeSourceOccurrences
Role: Buffer; Properties: Defined component, Organic compound, Simple component RAW_TEXT CultureMech
Role: Buffer; Properties: Defined component, Simple component, Organic compound RAW_TEXT CultureMech
Role: Buffer; Properties: Organic compound, Defined component, Simple component RAW_TEXT CultureMech
Role: Buffer; Properties: Organic compound, Simple component, Defined component RAW_TEXT CultureMech
Role: Buffer; Properties: Simple component, Defined component, Organic compound RAW_TEXT CultureMech
HEPES buffer EXACT_SYNONYM merged_from_duplicate_mim_record
Role: Buffer; Properties: Defined component, Solution RAW_TEXT CultureMech
HEPES buffer(CAS: 7365-45-9) RAW_TEXT CultureMech
HEPES buffer(Sigma H-3375) RAW_TEXT CultureMech
4-2-hydroxyethyl-1-piperazineethanesulfonic acid RAW_TEXT unmapped_duplicate_review_2026_05_11
4-(2-Hydroxyethyl)-1-piperazineethane sulfonic acid EXACT_SYNONYM local_chebi_exact_alias_review_2026_05_11
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid RAW_TEXT culturemech:output/ingredient_occurrences.tsv
HEPES (10mM) RAW_TEXT culturemech:output/ingredient_occurrences.tsv

Chemical properties

Cas rn
7365-45-9
Data source
PubChem API
Retrieval date
2026-04-05T19:52:22.089660+00:00

Evidence (2)

DATABASE_MATCH via CultureMech

Imported from CultureMech pipeline, quality=DIRECT_MATCH

DATABASE_MATCH via MIM curation (#320)

Re-grounded CHEBI:46756 -> CHEBI:42334. CHEBI:46756 is a structureless grouping class; the record's own cas_rn 7365-45-9 is registered in ChEBI against CHEBI:42334, which carries a structure and the trivial name 'HEPES' as a synonym. The label names one specific buffer, so §3 step 1 applies rather than the under-specified-parent rule.

Occurrence statistics

Total occurrences
251
Distinct media
251

Curation history

TimestampCuratorActionNew statusChanges
2026-03-09T06:54:18.022301+00:00 import_from_culturemech IMPORTED MAPPED Initial import from CultureMech pipeline
2026-04-05T19:52:22.089700+00:00 fetch_cas_rn_from_pubchem ADDED_CAS_RN MAPPED Added CAS-RN:7365-45-9 from PubChem API via name lookup (HEPES)
2026-04-05T21:48:45.176855+00:00 fetch_cas_rn_from_culturebot_csv ADDED_CAS_RN MAPPED Added CAS-RN:7365-45-9 from CultureBotHT compounds_to_cas.csv (matched: Hepes)
2026-04-19T01:05:11.444380+00:00 cbclaw_sssom_review_fix CORRECTED_ONTOLOGY_ID MAPPED Corrected ontology_id CHEBI:19708 -> CHEBI:46756 (old label: 'HEPES buffer', new label: 'HEPES'). Reason: Previous CHEBI:19708 is obsolete in current CHEBI release. Stripped 0 kg_microbe-sourced synonyms that were contaminated by the wrong CHEBI.
2026-04-19T07:39:23.955331+00:00 audit_mim_merge MERGED_FROM_DUPLICATES MAPPED Absorbed 2 duplicate MIM record(s) into this one: Hepes_Buffer.yaml, Hepes_2.yaml. Synonyms, curation_history, and occurrence counts were unioned/summed; winner selected by highest occurrence count with preferred_term readability tie-break.
2026-05-01T07:35:33.960640+00:00 auto_classify_ingredient_type AUTO_CLASSIFY_INGREDIENT_TYPE set ingredient_type=SINGLE_INGREDIENT (CHEBI primary)
2026-05-02T03:12:14.951394+00:00 auto_classify_ingredient_type AUTO_CLASSIFY_INGREDIENT_TYPE set ingredient_type=SINGLE_INGREDIENT (CHEBI primary)
2026-05-05T00:00:00+00:00 ingredient_mapping_review_2026_05_05 MERGED MAPPED Absorbed duplicate record Hepes_Buffer.yaml (UNMAPPED_0057) into Hepes.yaml; source occurrence counts and synonyms were merged. HEPES buffer text is preparation context; identity is HEPES CHEBI:46756.
2026-05-11T04:22:30Z mediaingredientmech-agentic-curation LLM MERGED_FROM_UNMAPPED_DUPLICATE MAPPED Absorbed duplicate unmapped record 4-2-hydroxyethyl-1-piperazineethanesulfonic_Acid.yaml (UNMAPPED_0319); local CHEBI aliases support the long-form HEPES surface text, and this mapped representative is already the project HEPES identity.
2026-05-29T23:35:36.111385+00:00 extract_roles_from_synonyms ANNOTATED Added media role: BUFFER (confidence: 1.00)
2026-07-20T00:00:00+00:00 migrate_media_roles_to_facets ANNOTATED Migrated legacy media_roles to role facets (#128): BUFFER -> physicochemical_roles.BUFFER
2026-08-13T00:00:00+00:00 reground_buffer_classes REGROUNDED_FROM_CLASS_TERM ontology_id CHEBI:46756 -> CHEBI:42334 '2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid' (#320); CAS 7365-45-9 confirms the specific term.
2026-08-27T03:16:39.518988+00:00 refresh_occurrence_statistics CORRECTED occurrence_statistics 70/168 -> 251/251 (media_count/total_occurrences), derived from distinct CultureMech recipe ids in the #337 occurrence table. The prior values came from the importer retired in #453, whose media_count counted a list truncated to 50 examples (#449). [source=ingredient_occurrences.tsv culturemech_rev=15a4da5c08 rows=194137 distinct_recipes=15257]
2026-08-30T04:48:06.233536+00:00 claude_culturemech_alias_backfill ADDED_SYNONYMS Added 2 CultureMech recipe surface form(s) that folded onto this record's published label but were absent from MIM's label index, so CultureMech could not resolve them: '4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid' (x1 CultureMech mentions); 'HEPES (10mM)' (x1 CultureMech mentions). Source: culturemech:output/ingredient_occurrences.tsv.
2026-09-09T00:00:00+00:00 fix_kg_microbe_node_id_drift CORRECTED kg_microbe_node_id CHEBI:46756 -> CHEBI:42334 (#554). The compatibility node id lagged a same-prefix identifier correction; the downstream node id must match the curated identifier unless a record intentionally crosses CURIE prefixes.