Marine broth 2216

Ontology mapping

Primary identifier: kgmicrobe.ingredient:marine_broth_2216
Notes: Imported from mim-queue (source_id=mediadive.ingredient:1607); no CAS-RN or CHEBI/NCIT match. Curator review needed.

Synonyms (5)

Synonym	Type	Source
Marine broth 2216	RAW_TEXT	mim-queue
Marine broth 2216 (BD-Difco)	CATALOG_VARIANT	culturemech:output/ingredient_occurrences.tsv
Marine Broth (MB) 2216 (Difco)	CATALOG_VARIANT	culturemech:output/ingredient_occurrences.tsv
Marine Broth 2216 (BD 279110)	CATALOG_VARIANT	culturemech:output/ingredient_occurrences.tsv
Marine Broth 2216 (BD--Difco)	CATALOG_VARIANT	culturemech:output/ingredient_occurrences.tsv

Evidence (1)

MANUAL_CURATION via MIM curation (#260)

Minted under the #288 named-preparation convention (#260): the broth twin of Marine Agar 2216, already in the corpus as an UNMAPPED record. Minted alongside its agar rather than left unmapped: the two differ only by the gelling agent, and splitting them would be arbitrary. Cross-reference: CultureMech:008094. A CultureMech id cannot be the identifier — MIM's primary key is an ontology CURIE or a registry mint — so it is carried here as evidence.

Material components

Method: RECIPE_TRANSCRIPTION
Completeness: COMPLETE

Component	Identifier	Scope	Concentration
Peptone	`MICRO:0000178`	MIM_CATALOG	5.0 G_PER_L
Yeast Extract	`FOODON:03315426`	MIM_CATALOG	1.0 G_PER_L
Ferric Citrate	`CHEBI:144421`	MIM_CATALOG	0.1 G_PER_L
Sodium Chloride	`CHEBI:26710`	MIM_CATALOG	19.45 G_PER_L
Magnesium Chloride	`CHEBI:86345`	MIM_CATALOG	8.8 G_PER_L
Sodium Sulfate	`CHEBI:32149`	MIM_CATALOG	3.24 G_PER_L
Calcium Chloride	`CHEBI:3312`	MIM_CATALOG	1.8 G_PER_L
Potassium Chloride	`CHEBI:32588`	MIM_CATALOG	0.55 G_PER_L
Sodium Bicarbonate	`CHEBI:32139`	MIM_CATALOG	0.16 G_PER_L
Potassium Bromide	`CHEBI:32030`	MIM_CATALOG	0.08 G_PER_L
Strontium Chloride	`CHEBI:36383`	MIM_CATALOG	0.034 G_PER_L
Boric Acid	`CHEBI:33118`	MIM_CATALOG	0.022 G_PER_L
Sodium Silicate	`CHEBI:60720`	MIM_CATALOG	0.004 G_PER_L
Sodium Fluoride	`CHEBI:28741`	MIM_CATALOG	0.0024 G_PER_L
Ammonium Nitrate	`CHEBI:63038`	MIM_CATALOG	0.0016 G_PER_L
Disodium Phosphate	`CHEBI:34683`	MIM_CATALOG	0.008 G_PER_L
Distilled water	`CHEBI:15377`	MIM_CATALOG	1.0 L_PER_L

Decomposition evidence

RECIPE_SOURCE https://www.atcc.org/~/media/5a4142bda5cf458b9131902a1791833d.ashx — ATCC Medium 2887 Marine Agar/Broth 2216

ATCC Medium 2887 lists the Difco Marine Agar 2216 scratch formula with peptone, yeast extract, ferric citrate, salts, agar, and distilled water per liter, and says to omit agar for broth medium.

Occurrence statistics

Total occurrences: 19
Distinct media: 19

Curation history

Timestamp	Curator	Action	New status	Changes
2026-04-30T18:43:03.698454+00:00	audit_import_ingredients[mim-queue]	CREATED_AS_UNMAPPED	UNMAPPED	Imported from mim-queue (source_id=mediadive.ingredient:1607); no CAS-RN or CHEBI/NCIT match. Curator review needed.
2026-05-02T03:12:19.725141+00:00	auto_classify_ingredient_type	AUTO_CLASSIFY_INGREDIENT_TYPE		set ingredient_type=UNDEFINED_MIXTURE (name matches complex pattern 'broth')
2026-07-05T00:00:00+00:00	cbclaw_media_modeling_114 LLM	RECLASSIFY_INGREDIENT_TYPE		ingredient_type UNDEFINED_MIXTURE -> DEFINED_MEDIUM: this is a complete culture-medium/agar/broth formulation (a medium), not an ingredient-level mixture.
2026-08-06T00:00:00+00:00	edison_literature_identity LLM	ANNOTATED		Edison LITERATURE (PaperQA3) identity research; report at research/ingredients/Marine_Broth_2216-edison-literature.md (local artifact — research/ is gitignored, so the findings are quoted here rather than linked). Report verdict: - Recommended mapping status: `UNMAPPED` No ontology CURIE was proposed. Recorded as provenance only — no grounding applied from this run. Edison's recommendations are deliberately conservative, and a plausible identifier promoted by a mechanism that never decided anything is what #203 and #263 exist to undo. Any CURIE above is a curator's to accept or reject.
2026-08-15T00:00:00+00:00	add_culturemech_gap_labels	PROMOTED_TO_MAPPED	MAPPED	UNMAPPED_0434 -> kgmicrobe.ingredient:marine_broth_2216 (FALLBACK_REGISTRY). Minted under the #288 named-preparation convention (#260): the broth twin of Marine Agar 2216, already in the corpus as an UNMAPPED record. Minted alongside its agar rather than left unmapped: the two differ only by the gelling agent, and splitting them would be arbitrary. Cross-reference: CultureMech:008094. A CultureMech id cannot be the identifier — MIM's primary key is an ontology CURIE or a registry mint — so it is carried here as evidence.
2026-08-27T03:16:39.517817+00:00	refresh_occurrence_statistics	CORRECTED		occurrence_statistics 0/0 -> 19/19 (media_count/total_occurrences), derived from distinct CultureMech recipe ids in the #337 occurrence table. The prior values came from the importer retired in #453, whose media_count counted a list truncated to 50 examples (#449). [source=ingredient_occurrences.tsv culturemech_rev=15a4da5c08 rows=194137 distinct_recipes=15257]
2026-08-30T04:48:06.233536+00:00	claude_culturemech_alias_backfill	ADDED_SYNONYMS		Added 4 CultureMech recipe surface form(s) that folded onto this record's published label but were absent from MIM's label index, so CultureMech could not resolve them: 'Marine broth 2216 (BD-Difco)' (x9 CultureMech mentions); 'Marine Broth (MB) 2216 (Difco)' (x1 CultureMech mentions); 'Marine Broth 2216 (BD 279110)' (x1 CultureMech mentions); 'Marine Broth 2216 (BD--Difco)' (x1 CultureMech mentions). Source: culturemech:output/ingredient_occurrences.tsv.
2026-09-06T00:00:00+00:00	mediaingredientmech-agentic-curation LLM	ANNOTATED		Added components and component_assertion by transcribing the ATCC Medium 2887 Difco Marine Agar 2216 scratch formula with agar omitted for broth.