Marine agar 2216

kgmicrobe.ingredient:marine_agar_2216 complex MAPPED kgmicrobe.ingredient FALLBACK_REGISTRY

Ontology mapping

Primary identifier
kgmicrobe.ingredient:marine_agar_2216

Synonyms (1)

SynonymTypeSourceOccurrences
Marine agar 2216 (BD-Difco) CATALOG_VARIANT culturemech:output/ingredient_occurrences.tsv

Evidence (1)

MANUAL_CURATION via MIM curation (#260)

Minted under the #288 named-preparation convention (#260): Difco/BD Marine Agar 2216, ZoBell's seawater formulation. MICRO has `ZoBell marine agar` (MICRO:0000112) and `216L marine agar` (MICRO:0000064), but neither is verified to be the 2216 formulation and grounding to a maybe-equivalent named medium is how wrong identities enter. No ChEBI/FOODON term covers a compounded medium either, so §3 step 3 mints it under the #288 convention, as `Trace element solution see Medium No. 187` already is. Cross-reference: CultureMech:007629. A CultureMech id cannot be the identifier — MIM's primary key is an ontology CURIE or a registry mint — so it is carried here as evidence.

Material components

Method
RECIPE_TRANSCRIPTION
Completeness
COMPLETE
ComponentIdentifierScopeConcentration
Peptone MICRO:0000178 MIM_CATALOG 5.0 G_PER_L
Yeast Extract FOODON:03315426 MIM_CATALOG 1.0 G_PER_L
Ferric Citrate CHEBI:144421 MIM_CATALOG 0.1 G_PER_L
Sodium Chloride CHEBI:26710 MIM_CATALOG 19.45 G_PER_L
Magnesium Chloride CHEBI:86345 MIM_CATALOG 8.8 G_PER_L
Sodium Sulfate CHEBI:32149 MIM_CATALOG 3.24 G_PER_L
Calcium Chloride CHEBI:3312 MIM_CATALOG 1.8 G_PER_L
Potassium Chloride CHEBI:32588 MIM_CATALOG 0.55 G_PER_L
Sodium Bicarbonate CHEBI:32139 MIM_CATALOG 0.16 G_PER_L
Potassium Bromide CHEBI:32030 MIM_CATALOG 0.08 G_PER_L
Strontium Chloride CHEBI:36383 MIM_CATALOG 0.034 G_PER_L
Boric Acid CHEBI:33118 MIM_CATALOG 0.022 G_PER_L
Sodium Silicate CHEBI:60720 MIM_CATALOG 0.004 G_PER_L
Sodium Fluoride CHEBI:28741 MIM_CATALOG 0.0024 G_PER_L
Ammonium Nitrate CHEBI:63038 MIM_CATALOG 0.0016 G_PER_L
Disodium Phosphate CHEBI:34683 MIM_CATALOG 0.008 G_PER_L
Agar CHEBI:2509 MIM_CATALOG 15.0 G_PER_L
Distilled water CHEBI:15377 MIM_CATALOG 1.0 L_PER_L

Decomposition evidence

RECIPE_SOURCE https://www.atcc.org/~/media/5a4142bda5cf458b9131902a1791833d.ashx — ATCC Medium 2887 Marine Agar/Broth 2216

ATCC Medium 2887 lists the Difco Marine Agar 2216 scratch formula with peptone, yeast extract, ferric citrate, salts, agar, and distilled water per liter.

Occurrence statistics

Total occurrences
32
Distinct media
32

Curation history

TimestampCuratorActionNew statusChanges
2026-08-15T00:00:00+00:00 add_culturemech_gap_labels CREATED_FROM_CULTUREMECH_GAP MAPPED kgmicrobe.ingredient:marine_agar_2216 -> kgmicrobe.ingredient:marine_agar_2216 (FALLBACK_REGISTRY). Minted under the #288 named-preparation convention (#260): Difco/BD Marine Agar 2216, ZoBell's seawater formulation. MICRO has `ZoBell marine agar` (MICRO:0000112) and `216L marine agar` (MICRO:0000064), but neither is verified to be the 2216 formulation and grounding to a maybe-equivalent named medium is how wrong identities enter. No ChEBI/FOODON term covers a compounded medium either, so §3 step 3 mints it under the #288 convention, as `Trace element solution see Medium No. 187` already is. Cross-reference: CultureMech:007629. A CultureMech id cannot be the identifier — MIM's primary key is an ontology CURIE or a registry mint — so it is carried here as evidence.
2026-08-27T03:16:39.518115+00:00 refresh_occurrence_statistics CORRECTED occurrence_statistics 0/0 -> 32/32 (media_count/total_occurrences), derived from distinct CultureMech recipe ids in the #337 occurrence table. The prior values came from the importer retired in #453, whose media_count counted a list truncated to 50 examples (#449). [source=ingredient_occurrences.tsv culturemech_rev=15a4da5c08 rows=194137 distinct_recipes=15257]
2026-08-30T04:48:06.233536+00:00 claude_culturemech_alias_backfill ADDED_SYNONYMS Added 1 CultureMech recipe surface form(s) that folded onto this record's published label but were absent from MIM's label index, so CultureMech could not resolve them: 'Marine agar 2216 (BD-Difco)' (x6 CultureMech mentions). Source: culturemech:output/ingredient_occurrences.tsv.
2026-09-06T00:00:00+00:00 mediaingredientmech-agentic-curation LLM ANNOTATED Added components and component_assertion by transcribing the ATCC Medium 2887 Difco Marine Agar 2216 scratch formula.