Mineral 3B solution

kgmicrobe.ingredient:mineral_3b_solution defined mix MAPPED kgmicrobe.ingredient FALLBACK_REGISTRY

Ontology mapping

Primary identifier
kgmicrobe.ingredient:mineral_3b_solution
Notes
Imported from CultureBotHT (consolidated_media.json (media-only)). Used in 9 CultureBot media; samples: Varel_Bryant_medium, Varel_Bryant_medium_lowCys, Varel_Bryant_medium_lowCys_lowMet…. No CAS-RN or CHEBI mapping available; reviewed on 2026-09-06 as a mineral stock solution and transcribed from the Bacic and Smith 2008 Mineral 3B solution recipe.

Nutritional roles

Synonyms (1)

SynonymTypeSourceOccurrences
Mineral 3B solution RAW_TEXT culturebotht

Evidence (1)

DATABASE_MATCH via MIM curation (#114)

Mineral 3B solution is a named, recurring multi-component preparation (9 media). Searched CHEBI, NCIT, MeSH, FOODON and ENVO by label and synonym with normalisation: no term denotes it, and none could -- it is a lab preparation rather than a substance. Takes the STOCK_SOLUTION convention used by the existing mapped records: a kgmicrobe.ingredient: mint, with no parent because a multi-component mix does not narrow to any single compound (#114).

Material components

Method
RECIPE_TRANSCRIPTION
Completeness
COMPLETE
ComponentIdentifierScopeConcentration
KH2PO4 CHEBI:63036 MIM_CATALOG 18 G_PER_L
NaCl CHEBI:26710 MIM_CATALOG 18 G_PER_L
MgCl2 x 6 H2O CHEBI:86345 MIM_CATALOG 0.4 G_PER_L
CaCl2 x 2 H2O CHEBI:86158 MIM_CATALOG 0.52 G_PER_L
CoCl2 x 6 H2O CHEBI:53503 MIM_CATALOG 0.02 G_PER_L
MnCl2 x 4 H2O CHEBI:86368 MIM_CATALOG 0.2 G_PER_L
NH4Cl CHEBI:31206 MIM_CATALOG 10.0 G_PER_L
Na2SO4 CHEBI:32149 MIM_CATALOG 5.0 G_PER_L
Distilled water CHEBI:15377 MIM_CATALOG 1.0 L_PER_L

Decomposition evidence

RECIPE_SOURCE https://pmc.ncbi.nlm.nih.gov/articles/PMC3836205/ — Bacic and Smith 2008 Mineral 3B solution

Bacic and Smith list KH2PO4, NaCl, MgCl2 x 6 H2O, CaCl2 x 2 H2O, CoCl2 x 6 H2O, MnCl2 x 4 H2O, NH4Cl, and Na2SO4 per liter, and instruct dissolving them in 800 ml dH2O before bringing the solution to 1 liter.

Occurrence statistics

Total occurrences
9
Distinct media
9

Curation history

TimestampCuratorActionNew statusChanges
2026-04-29T06:51:33.117170+00:00 audit_add_remaining_culturebotht CREATED_AS_UNMAPPED UNMAPPED Imported from CultureBotHT (consolidated_media.json (media-only)). Used in 9 CultureBot media; samples: Varel_Bryant_medium, Varel_Bryant_medium_lowCys, Varel_Bryant_medium_lowCys_lowMet…. No CAS-RN or CHEBI mapping available; curator review needed.
2026-05-11T09:05:00Z mediaingredientmech-agentic-curation LLM CLASSIFIED_STOCK_SOLUTION UNMAPPED Classified as a mineral stock solution and left unmapped pending component-level recipe curation.
2026-08-06T00:00:00+00:00 promote_resolved_unmapped PROMOTED_TO_MAPPED MAPPED Promoted UNMAPPED_0262 -> kgmicrobe.ingredient:mineral_3b_solution "Mineral 3B solution" (FALLBACK_REGISTRY); mapping_status UNMAPPED -> MAPPED, SSSOM row added.
2026-09-06T00:00:00+00:00 mediaingredientmech-agentic-curation LLM ANNOTATED Added mineral, phosphate, nitrogen, sulfur, and trace-element roles plus components and component_assertion by transcribing the Bacic and Smith 2008 Mineral 3B solution formulation.