Mineral 3B solution minus Nitrogen

Ontology mapping

Primary identifier: kgmicrobe.ingredient:mineral_3b_solution_minus_nitrogen
Notes: Imported from CultureBotHT (consolidated_media.json (media-only)). Used in 5 CultureBot media; samples: Varel_Bryant_medium_Glucose_lowCys_noNitrogen, Varel_Bryant_medium_Glucose_DTT_noNitrogen, Varel_Bryant_medium_Glucose_DTT_NaS_noNitrogen…. No CAS-RN or CHEBI mapping available; reviewed on 2026-05-11 as a nitrogen-free mineral stock solution variant pending component-level recipe curation. Component-level recipe transcribed from the CultureBotHT media definitions Mixes tab on 2026-09-08.

Nutritional roles

Role
MINERAL_SOURCE
Confidence
1.0
Evidence
- Reference text
  CultureBotHT media definitions Mixes tab
  Reference type
  DATABASE_ENTRY
  Url
  https://docs.google.com/spreadsheets/d/1lNNW8BtrJL3pBlsnwvrQRKLU-9asECU_qaBfZ36Ft84
  Excerpt
  Mineral 3B solution minus Nitrogen
  Curator note
  Mineral 3B solution minus Nitrogen is a stock of inorganic potassium, sodium, magnesium, calcium, cobalt, manganese, phosphate, and sulfate salts for nitrogen-omission media.

Synonyms (1)

Synonym	Type	Source	Occurrences
Mineral 3B solution minus Nitrogen	RAW_TEXT	culturebotht

Evidence (1)

DATABASE_MATCH via MIM curation (#114)

Mineral 3B solution minus Nitrogen is a named, recurring multi-component preparation (5 media). Searched CHEBI, NCIT, MeSH, FOODON and ENVO by label and synonym with normalisation: no term denotes it, and none could -- it is a lab preparation rather than a substance. Takes the STOCK_SOLUTION convention used by the existing mapped records: a kgmicrobe.ingredient: mint, with no parent because a multi-component mix does not narrow to any single compound (#114).

Material components

Method: RECIPE_TRANSCRIPTION
Completeness: COMPLETE

Component	Identifier	Scope	Concentration
Potassium phosphate monobasic	`CHEBI:63036`	MIM_CATALOG	6.6 MM
Sodium Chloride	`CHEBI:26710`	MIM_CATALOG	15.4 MM
Magnesium chloride hexahydrate	`CHEBI:86345`	MIM_CATALOG	98.0 UM
Calcium chloride dihydrate	`CHEBI:86158`	MIM_CATALOG	176.5 UM
Cobalt chloride hexahydrate	`CHEBI:53503`	MIM_CATALOG	4.2 UM
Manganese (II) chloride tetrahydrate	`CHEBI:86368`	MIM_CATALOG	50.5 UM
Sodium sulfate	`CHEBI:32149`	MIM_CATALOG	1.75 MM

Decomposition evidence

RECIPE_SOURCE https://docs.google.com/spreadsheets/d/1lNNW8BtrJL3pBlsnwvrQRKLU-9asECU_qaBfZ36Ft84 — Mixes!156:162 Mineral 3B solution minus Nitrogen

CultureBotHT's media definitions Mixes tab defines Mineral 3B solution minus Nitrogen as a 7-component mineral stock containing potassium phosphate, sodium, magnesium, calcium, cobalt, manganese, and sulfate salts, with no ammonium salt.

Occurrence statistics

Total occurrences: 5
Distinct media: 5

Curation history

Timestamp	Curator	Action	New status	Changes
2026-04-29T06:51:33.117170+00:00	audit_add_remaining_culturebotht	CREATED_AS_UNMAPPED	UNMAPPED	Imported from CultureBotHT (consolidated_media.json (media-only)). Used in 5 CultureBot media; samples: Varel_Bryant_medium_Glucose_lowCys_noNitrogen, Varel_Bryant_medium_Glucose_DTT_noNitrogen, Varel_Bryant_medium_Glucose_DTT_NaS_noNitrogen…. No CAS-RN or CHEBI mapping available; curator review needed.
2026-05-11T09:30:00Z	mediaingredientmech-agentic-curation LLM	CLASSIFIED_STOCK_SOLUTION	UNMAPPED	Classified as a nitrogen-free mineral stock solution variant and left unmapped pending component-level recipe curation.
2026-08-06T00:00:00+00:00	promote_resolved_unmapped	PROMOTED_TO_MAPPED	MAPPED	Promoted UNMAPPED_0254 -> kgmicrobe.ingredient:mineral_3b_solution_minus_nitrogen "Mineral 3B solution minus Nitrogen" (FALLBACK_REGISTRY); mapping_status UNMAPPED -> MAPPED, SSSOM row added.
2026-09-08T00:00:00+00:00	mediaingredientmech-agentic-curation LLM	ANNOTATED	MAPPED	Transcribed the CultureBotHT Mixes-tab recipe for Mineral 3B solution minus Nitrogen and added complete component partonomy for its 7 mineral-stock constituents plus a source-backed mineral-source role.