Modified Wolfe's Minerals

Ontology mapping

Primary identifier: kgmicrobe.ingredient:modified_wolfes_minerals
Notes: Imported from CultureBotHT (consolidated_media.json (media-only)). Used in 3 CultureBot media; samples: MG minimal media, MG minimal media_noIron, MG minimal media_noCarbon. No CAS-RN or CHEBI mapping available; reviewed on 2026-05-11 as a modified Wolfe mineral stock/pre-mix pending component-level recipe curation. Component-level recipe transcribed from the CultureBotHT media definitions Mixes tab on 2026-09-08.

Nutritional roles

Role
MINERAL_SOURCE
Confidence
1.0
Evidence
- Reference text
  CultureBotHT media definitions Mixes tab
  Reference type
  DATABASE_ENTRY
  Url
  https://docs.google.com/spreadsheets/d/1lNNW8BtrJL3pBlsnwvrQRKLU-9asECU_qaBfZ36Ft84
  Excerpt
  Modified Wolfe's Minerals
  Curator note
  Modified Wolfe's Minerals is a stock of inorganic mineral and trace salts chelated by nitrilotriacetic acid for MG minimal media variants.

Synonyms (1)

Synonym	Type	Source	Occurrences
Modified Wolfe's Minerals	RAW_TEXT	culturebotht

Evidence (1)

DATABASE_MATCH via MIM curation (#114)

Modified Wolfe's Minerals is a named, recurring multi-component preparation (3 media). Searched CHEBI, NCIT, MeSH, FOODON and ENVO by label and synonym with normalisation: no term denotes it, and none could -- it is a lab preparation rather than a substance. Takes the STOCK_SOLUTION convention used by the existing mapped records: a kgmicrobe.ingredient: mint, with no parent because a multi-component mix does not narrow to any single compound (#114).

Material components

Method: RECIPE_TRANSCRIPTION
Completeness: COMPLETE

Component	Identifier	Scope	Concentration
Nitrilotriacetic acid	`CHEBI:44557`	MIM_CATALOG	1.5 G_PER_L
Magnesium sulfate	`CHEBI:32599`	MIM_CATALOG	1.465 G_PER_L
Manganese (II) sulfate monohydrate	`CHEBI:86364`	MIM_CATALOG	0.5 G_PER_L
Sodium Chloride	`CHEBI:26710`	MIM_CATALOG	1.0 G_PER_L
Cobalt chloride hexahydrate	`CHEBI:53503`	MIM_CATALOG	0.1 G_PER_L
Calcium Chloride Dihydrate	`CHEBI:86158`	MIM_CATALOG	0.1 G_PER_L
Zinc sulfate heptahydrate	`CHEBI:32312`	MIM_CATALOG	0.1 G_PER_L
Aluminum potassium sulfate dodecahydrate	`CHEBI:86465`	MIM_CATALOG	0.02 G_PER_L
Boric Acid	`CHEBI:33118`	MIM_CATALOG	0.01 G_PER_L
Sodium Molybdate Dihydrate	`CHEBI:75213`	MIM_CATALOG	0.01 G_PER_L

Decomposition evidence

RECIPE_SOURCE https://docs.google.com/spreadsheets/d/1lNNW8BtrJL3pBlsnwvrQRKLU-9asECU_qaBfZ36Ft84 — Mixes!206:215 Modified Wolfe's Minerals

CultureBotHT's media definitions Mixes tab defines Modified Wolfe's Minerals as a 10-component stock containing nitrilotriacetic acid, magnesium, manganese, sodium, cobalt, calcium, zinc, aluminum potassium sulfate, boric acid, and molybdate salts.

Occurrence statistics

Total occurrences: 3
Distinct media: 3

Curation history

Timestamp	Curator	Action	New status	Changes
2026-04-29T06:51:33.117170+00:00	audit_add_remaining_culturebotht	CREATED_AS_UNMAPPED	UNMAPPED	Imported from CultureBotHT (consolidated_media.json (media-only)). Used in 3 CultureBot media; samples: MG minimal media, MG minimal media_noIron, MG minimal media_noCarbon. No CAS-RN or CHEBI mapping available; curator review needed.
2026-05-11T10:20:00Z	mediaingredientmech-agentic-curation LLM	CLASSIFIED_STOCK_SOLUTION	UNMAPPED	Classified as a modified Wolfe mineral stock/pre-mix and left unmapped pending component-level recipe curation.
2026-08-06T00:00:00+00:00	promote_resolved_unmapped	PROMOTED_TO_MAPPED	MAPPED	Promoted UNMAPPED_0314 -> kgmicrobe.ingredient:modified_wolfes_minerals "Modified Wolfe's Minerals" (FALLBACK_REGISTRY); mapping_status UNMAPPED -> MAPPED, SSSOM row added.
2026-09-08T00:00:00+00:00	mediaingredientmech-agentic-curation LLM	ANNOTATED	MAPPED	Transcribed the CultureBotHT Mixes-tab recipe for Modified Wolfe's Minerals and added complete component partonomy for its 10 mineral-stock constituents plus a source-backed mineral-source role.