NLDM_metabolites

Ontology mapping

Primary identifier: kgmicrobe.ingredient:nldm_metabolites
Notes: Imported from CultureBotHT (consolidated_media.json (media-only)). Used in 10 CultureBot media; samples: NLDM_defined_no_iron, NLDM_defined, NLDM_defined_pH8…. No CAS-RN or CHEBI mapping available; reviewed on 2026-05-11 as a named metabolite pre-mix. Component-level recipe transcribed from the CultureBotHT Mixes tab on 2026-09-08.

Nutritional roles

Role
CARBON_SOURCE
Confidence
1.0
Evidence
- Reference text
  CultureBotHT media definitions Mixes tab
  Reference type
  DATABASE_ENTRY
  Url
  https://docs.google.com/spreadsheets/d/1lNNW8BtrJL3pBlsnwvrQRKLU-9asECU_qaBfZ36Ft84
  Excerpt
  NLDM_metabolites
  Curator note
  CultureBotHT enumerates sugars, organic acids, amino acids, nucleobases, nucleosides, and related carbon-containing metabolites in NLDM_metabolites.
Role
NITROGEN_SOURCE
Confidence
1.0
Evidence
- Reference text
  CultureBotHT media definitions Mixes tab
  Reference type
  DATABASE_ENTRY
  Url
  https://docs.google.com/spreadsheets/d/1lNNW8BtrJL3pBlsnwvrQRKLU-9asECU_qaBfZ36Ft84
  Excerpt
  NLDM_metabolites
  Curator note
  CultureBotHT enumerates amino acids, nucleobases, nucleosides, and related nitrogen-containing metabolites in NLDM_metabolites.
Role
SULFUR_SOURCE
Confidence
1.0
Evidence
- Reference text
  CultureBotHT media definitions Mixes tab
  Reference type
  DATABASE_ENTRY
  Url
  https://docs.google.com/spreadsheets/d/1lNNW8BtrJL3pBlsnwvrQRKLU-9asECU_qaBfZ36Ft84
  Excerpt
  NLDM_metabolites
  Curator note
  CultureBotHT includes sulfur-containing L-Cysteine, L-Methionine, and Taurine among the NLDM_metabolites constituents.
Role
PHOSPHATE_SOURCE
Confidence
1.0
Evidence
- Reference text
  CultureBotHT media definitions Mixes tab
  Reference type
  DATABASE_ENTRY
  Url
  https://docs.google.com/spreadsheets/d/1lNNW8BtrJL3pBlsnwvrQRKLU-9asECU_qaBfZ36Ft84
  Excerpt
  NLDM_metabolites
  Curator note
  CultureBotHT includes sn-glycero-3-phosphocholine as a phosphorus-containing NLDM_metabolites constituent.

Synonyms (1)

Synonym	Type	Source	Occurrences
NLDM_metabolites	RAW_TEXT	culturebotht

Evidence (1)

DATABASE_MATCH via MIM curation (#114)

NLDM_metabolites is a named, recurring multi-component preparation (10 media). Searched CHEBI, NCIT, MeSH, FOODON and ENVO by label and synonym with normalisation: no term denotes it, and none could -- it is a lab preparation rather than a substance. Takes the STOCK_SOLUTION convention used by the existing mapped records: a kgmicrobe.ingredient: mint, with no parent because a multi-component mix does not narrow to any single compound (#114).

Material components

Method: RECIPE_TRANSCRIPTION
Completeness: COMPLETE

Component	Identifier	Scope	Concentration
Sucrose	`CHEBI:17992`	MIM_CATALOG	875.0 UM
D-Glucose	`CHEBI:17634`	MIM_CATALOG	875.0 UM
D-Trehalose dihydrate	`CHEBI:232797`	MIM_CATALOG	875.0 UM
m-Inositol	`CHEBI:17268`	MIM_CATALOG	875.0 UM
D-Xylose	`CHEBI:65327`	MIM_CATALOG	875.0 UM
D-Mannitol	`CHEBI:16899`	MIM_CATALOG	875.0 UM
N-Acetyl-D-Glucosamine	`CHEBI:506227`	MIM_CATALOG	875.0 UM
Sodium L-Lactate	`CHEBI:232798`	MIM_CATALOG	525.0 UM
D,L-Malic Acid	`CHEBI:6650`	MIM_CATALOG	525.0 UM
Citric Acid	`CHEBI:30769`	MIM_CATALOG	525.0 UM
Sodium succinate dibasic hexahydrate	`CHEBI:63686`	MIM_CATALOG	525.0 UM
Sodium pyruvate	`CHEBI:50144`	MIM_CATALOG	525.0 UM
a-Ketoglutaric acid disodium salt hydrate	`cas:305-72-6`	MIM_CATALOG	525.0 UM
L-Citrulline	`CHEBI:16349`	MIM_CATALOG	525.0 UM
L-Alanine	`CHEBI:16977`	MIM_CATALOG	175.0 UM
L-Arginine	`CHEBI:16467`	MIM_CATALOG	175.0 UM
L-Asparagine	`CHEBI:17196`	MIM_CATALOG	175.0 UM
L-Aspartic acid	`CHEBI:17053`	MIM_CATALOG	175.0 UM
L-Cysteine	`CHEBI:17561`	MIM_CATALOG	175.0 UM
L-Glutamic acid	`CHEBI:16015`	MIM_CATALOG	175.0 UM
L-Glutamine	`CHEBI:18050`	MIM_CATALOG	175.0 UM
Glycine	`CHEBI:15428`	MIM_CATALOG	175.0 UM
L-Histidine	`CHEBI:15971`	MIM_CATALOG	175.0 UM
L-Isoleucine	`CHEBI:17191`	MIM_CATALOG	175.0 UM
L-Leucine	`CHEBI:15603`	MIM_CATALOG	175.0 UM
L-Lysine	`CHEBI:18019`	MIM_CATALOG	175.0 UM
L-Methionine	`CHEBI:16643`	MIM_CATALOG	175.0 UM
L-Phenylalanine	`CHEBI:17295`	MIM_CATALOG	175.0 UM
L-Proline	`CHEBI:17203`	MIM_CATALOG	175.0 UM
L-Serine	`CHEBI:17115`	MIM_CATALOG	175.0 UM
L-Threonine	`CHEBI:16857`	MIM_CATALOG	175.0 UM
L-Tryptophan	`CHEBI:16828`	MIM_CATALOG	175.0 UM
L-tyrosine	`CHEBI:17895`	MIM_CATALOG	175.0 UM
L-Valine	`CHEBI:16414`	MIM_CATALOG	175.0 UM
5-methyluridine	`CHEBI:45996`	MIM_CATALOG	17.5 UM
Adenine hydrochloride hydrate	`cas:2922-28-3`	MIM_CATALOG	17.5 UM
Adenosine	`CHEBI:16335`	MIM_CATALOG	17.5 UM
Cytidine	`CHEBI:17562`	MIM_CATALOG	17.5 UM
Cytosine	`CHEBI:16040`	MIM_CATALOG	17.5 UM
Guanine	`CHEBI:16235`	MIM_CATALOG	17.5 UM
Guanosine	`CHEBI:16750`	MIM_CATALOG	17.5 UM
Hypoxanthine	`CHEBI:17368`	MIM_CATALOG	17.5 UM
Inosine	`CHEBI:17596`	MIM_CATALOG	17.5 UM
Thymine	`CHEBI:17821`	MIM_CATALOG	17.5 UM
Uracil	`CHEBI:17568`	MIM_CATALOG	17.5 UM
Uridine	`CHEBI:16704`	MIM_CATALOG	17.5 UM
Xanthine	`CHEBI:17712`	MIM_CATALOG	17.5 UM
Xanthosine	`CHEBI:18107`	MIM_CATALOG	17.5 UM
Gamma-Aminobutyric Acid Hydrochloride	unmapped	UNMAPPED	17.5 UM
Ectoine	`CHEBI:27592`	MIM_CATALOG	17.5 UM
Trimethylglycine	unmapped	UNMAPPED	17.5 UM
N-Acetyl-glutamic acid	`CHEBI:172431`	MIM_CATALOG	17.5 UM
Nicotinamide	`CHEBI:17154`	MIM_CATALOG	17.5 UM
L-Ornithine	`CHEBI:15729`	MIM_CATALOG	17.5 UM
Shikimic Acid	`CHEBI:16119`	MIM_CATALOG	17.5 UM
spermidine	`CHEBI:16610`	MIM_CATALOG	17.5 UM
Taurine	`CHEBI:15891`	MIM_CATALOG	17.5 UM
Trigonelline HCl	`CHEBI:229203`	MIM_CATALOG	17.5 UM
Carnitine Hydrochloride	`kgmicrobe.compound:carnitine_hydrochloride`	MIM_CATALOG	17.5 UM
Choline chloride	`CHEBI:133341`	MIM_CATALOG	17.5 UM
n-Acetyl-glutamine	`CHEBI:73685`	MIM_CATALOG	17.5 UM
n-Acetyl-lysine	`CHEBI:35704`	MIM_CATALOG	17.5 UM
n-Acetyl-muramic acid	`CHEBI:47965`	MIM_CATALOG	17.5 UM
sn-glycero-3-phosphocholine	`CHEBI:16870`	MIM_CATALOG	17.5 UM

Decomposition evidence

RECIPE_SOURCE https://docs.google.com/spreadsheets/d/1lNNW8BtrJL3pBlsnwvrQRKLU-9asECU_qaBfZ36Ft84 — Mixes!786:849 NLDM_metabolites

CultureBotHT's media definitions Mixes tab defines NLDM_metabolites as a 64-component stock used at 1X.

Occurrence statistics

Total occurrences: 10
Distinct media: 10

Curation history

Timestamp	Curator	Action	New status	Changes
2026-04-29T06:51:33.117170+00:00	audit_add_remaining_culturebotht	CREATED_AS_UNMAPPED	UNMAPPED	Imported from CultureBotHT (consolidated_media.json (media-only)). Used in 10 CultureBot media; samples: NLDM_defined_no_iron, NLDM_defined, NLDM_defined_pH8…. No CAS-RN or CHEBI mapping available; curator review needed.
2026-05-11T09:05:00Z	mediaingredientmech-agentic-curation LLM	CLASSIFIED_STOCK_SOLUTION	UNMAPPED	Classified as a named metabolite pre-mix and left unmapped pending component-level recipe curation.
2026-08-06T00:00:00+00:00	promote_resolved_unmapped	PROMOTED_TO_MAPPED	MAPPED	Promoted UNMAPPED_0299 -> kgmicrobe.ingredient:nldm_metabolites "NLDM_metabolites" (FALLBACK_REGISTRY); mapping_status UNMAPPED -> MAPPED, SSSOM row added.
2026-09-08T00:00:00+00:00	mediaingredientmech-agentic-curation LLM	ANNOTATED		Transcribed NLDM_metabolites' 64 CultureBotHT Mixes-tab constituents with component concentrations, left Gamma-Aminobutyric Acid Hydrochloride and Trimethylglycine unresolved to avoid unsafe free-acid or hydrate substitutions, and annotated carbon, nitrogen, sulfur, and phosphate source roles.