nitrous oxide

Ontology mapping

Primary identifier: CHEBI:17045
Ontology label: dinitrogen oxide

Cellular metabolic roles

Role
ELECTRON_ACCEPTOR
Confidence
0.6
Evidence
- Reference type
  COMPUTATIONAL_PREDICTION
  Reference text
  Assigned by in-session Claude reasoning (no external API)
  Curator note
  Provisional in-session LLM role assignment; review recommended.
- Reference type
  PEER_REVIEWED_PUBLICATION
  Doi
  10.1128/AEM.00409-16
  Pmid
  27084012
  Url
  https://pmc.ncbi.nlm.nih.gov/articles/PMC4907195/
  Reference text
  Yoon et al. (2016). Nitrous oxide reduction kinetics distinguish bacteria harboring clade I NosZ from those harboring clade II NosZ.
  Curator note
  Growth and N2O consumption were measured together in the named isolates with acetate supplying electrons. The role is confined to these tested nitrous-oxide-respiring cultures.
Metabolic context
Terminal electron acceptor during anoxic, acetate-fed growth of Dechloromonas aromatica RCB, Anaeromyxobacter dehalogenans 2CP-C, Pseudomonas stutzeri DCP-Ps1, and Shewanella loihica PV-4 at 30 degrees C.

Synonyms (3)

Synonym	Type	Source
N2O	EXACT_SYNONYM	unmapped_duplicate_review_2026_05_11
#N2O	RAW_TEXT	unmapped_duplicate_review_2026_05_11
oxidodinitrogen(N--N)	EXACT_SYNONYM	chebi_synonym_review

Chemical properties

Cas rn: 10024-97-2
Data source: CultureBotHT compounds_to_cas.csv
Retrieval date: 2026-04-29T06:34:31.158938+00:00
Molecular formula: N2O
Inchi: InChI=1S/N2O/c1-2-3
Smiles: N#[N+][O-]

Evidence (1)

DATABASE_MATCH via CultureBotHT

Imported from CultureBotHT. CAS-RN→CHEBI mapping resolved via OAK CHEBI sqlite (cas xref).

Occurrence statistics

Total occurrences: 3
Distinct media: 3

Curation history

Timestamp	Curator	Action	New status	Changes
2026-04-29T06:34:31.158938+00:00	audit_cas_chebi_followup	CREATED_FROM_CAS_LOOKUP	MAPPED	Created from CultureBotHT CAS-RN=10024-97-2; CHEBI resolved via OAK CHEBI sqlite (cas xref); Imported from CultureBotHT. CAS-RN→CHEBI mapping resolved via OAK CHEBI sqlite (cas xref).
2026-05-01T07:35:35.597945+00:00	auto_classify_ingredient_type	AUTO_CLASSIFY_INGREDIENT_TYPE		set ingredient_type=SINGLE_INGREDIENT (CHEBI primary)
2026-05-01T08:08:31.701480+00:00	auto_classify_ingredient_type	AUTO_BACKFILL_CHEBI_CHEMISTRY		molecular_formula=N2O; inchi=InChI=1S/N2O/c1-2-3; smiles=N#[N+][O-]
2026-05-02T03:12:17.495928+00:00	auto_classify_ingredient_type	AUTO_CLASSIFY_INGREDIENT_TYPE		set ingredient_type=SINGLE_INGREDIENT (chemical structure populated (molecular_formula))
2026-05-11T04:17:00Z	mediaingredientmech-agentic-curation LLM	MERGED_FROM_UNMAPPED_DUPLICATE	MAPPED	Absorbed duplicate unmapped record N2O.yaml (UNMAPPED_0296); CultureBotHT surface form '#N2O' denotes nitrous oxide in N2O/argon media names, and local CHEBI plus SSSOM review confirms CHEBI:17045 as dinitrogen oxide.
2026-06-09T04:56:30.248125+00:00	claude_in_session_curation	ANNOTATED		Added media role: ELECTRON_ACCEPTOR (confidence: 0.60)
2026-07-20T00:00:00+00:00	migrate_media_roles_to_facets	ANNOTATED		Migrated legacy media_roles to role facets (#128): ELECTRON_ACCEPTOR -> cellular_metabolic_roles.ELECTRON_ACCEPTOR
2026-08-10T00:00:00+00:00	regrade_synonym_matches	REGRADED_EXACT_TO_SYNONYM		mapping_quality EXACT_MATCH -> SYNONYM_MATCH (#317). The record's label does not equal the term's primary label 'dinitrogen oxide'; it matches the ChEBI synonym 'N2O' on CHEBI:17045. The schema defines EXACT_MATCH as a direct match to the term and SYNONYM_MATCH as a match to a known synonym, so the recorded grade overstated. ontology_id is unchanged — the term is correct. The SSSOM predicate is unaffected (both grades emit skos:exactMatch).
2026-08-24T00:00:00+00:00	regrade_synonym_matches	REGRADED_TO_CAS_RN_LOOKUP		mapping_quality SYNONYM_MATCH -> CAS_RN_LOOKUP (#317). The record was created by an explicit CAS-to-ChEBI lookup and its current CAS RN 10024-97-2 uniquely resolves by ChEBI xref to CHEBI:17045 'dinitrogen oxide'. CAS_RN_LOOKUP records the method that established this mapping; a lexical grade would discard that provenance. ontology_id is unchanged. The own-identifier SSSOM predicate remains skos:exactMatch under Rule D (#438), independently of mapping quality.
2026-08-27T03:16:39.519641+00:00	refresh_occurrence_statistics	CORRECTED		occurrence_statistics 4/4 -> 3/3 (media_count/total_occurrences), derived from distinct CultureMech recipe ids in the #337 occurrence table. The prior values came from the importer retired in #453, whose media_count counted a list truncated to 50 examples (#449). [source=ingredient_occurrences.tsv culturemech_rev=15a4da5c08 rows=194137 distinct_recipes=15257]
2026-09-22T02:06:34+00:00	codex_issue_710_semantic_evidence_review LLM	CORRECTED_ROLE_EVIDENCE		Added inspected primary-publication support and bounded organism/condition context to the cellular metabolic role (#710). Retained original computational provenance and confidence. See reports/semantic_review_20260921/resolution/roles/cellular-role-plan.json for the assertion-specific interpretation.