Zeikus trace element solution

Ontology mapping

Primary identifier: kgmicrobe.ingredient:zeikus_trace_element_solution
Ontology term: NCIT:C896 — Trace Element
Ontology label: Trace Element
Notes: Imported from mim-queue (source_id=mediadive.ingredient:1914); no CAS-RN or CHEBI/NCIT match. Curator review needed.
kg-microbe node: kgmicrobe.ingredient:zeikus_trace_element_solution

Nutritional roles

Role
TRACE_ELEMENT
Confidence
1.0
Evidence
- Reference text
  JCM Medium No. 684 Trace mineral solution
  Reference type
  TECHNICAL_REPORT
  Url
  https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=684
  Excerpt
  Trace mineral solution:
  Curator note
  JCM medium J849's Zeikus trace element solution refers to JCM medium 684, whose Trace mineral solution contains iron, manganese, cobalt, calcium, zinc, copper, molybdenum, and selenium salts plus boric acid.

Synonyms (1)

Synonym	Type	Source	Occurrences
Zeikus trace element solution	RAW_TEXT	mim-queue

Evidence (2)

DATABASE_MATCH via NCIT via OLS (resolve_unmapped_v2 strategy=stem-match)

Auto-upgraded from UNMAPPED_0584; matched via 'Zeikus trace element solution'; stem-substring

CURATOR_JUDGMENT via mediaingredientmech-agentic-curation

Zeikus trace element solution is a named stock formulation; NCIT:C896 is retained only as a broad trace-element category.

Material components

Method: RECIPE_TRANSCRIPTION
Completeness: COMPLETE

Component	Identifier	Scope	Concentration
Nitrilotriacetic acid	`CHEBI:44557`	MIM_CATALOG	12.5 G_PER_L
FeCl2 x 4 H2O	`CHEBI:86249`	MIM_CATALOG	0.2 G_PER_L
MnCl2 x 4 H2O	`CHEBI:86368`	MIM_CATALOG	0.1 G_PER_L
CoCl2 x 6 H2O	`CHEBI:53503`	MIM_CATALOG	0.02 G_PER_L
CaCl2 x 2 H2O	`CHEBI:86158`	MIM_CATALOG	0.1 G_PER_L
ZnCl2	`CHEBI:49976`	MIM_CATALOG	0.1 G_PER_L
CuCl2	`CHEBI:49553`	MIM_CATALOG	0.2 G_PER_L
H3BO3	`CHEBI:33118`	MIM_CATALOG	0.01 G_PER_L
Na2MoO4 x 2 H2O	`CHEBI:75213`	MIM_CATALOG	0.01 G_PER_L
NaCl	`CHEBI:26710`	MIM_CATALOG	1.0 G_PER_L
Na2SeO3 x 5 H2O	`CHEBI:131361`	MIM_CATALOG	0.02 G_PER_L
Distilled water	`CHEBI:15377`	MIM_CATALOG	1000.0 ML_PER_L

Decomposition evidence

RECIPE_SOURCE https://mediadive.dsmz.de/download/medium/J849/json — JCM medium J849 row 8, compound_id 1914

JCM medium J849 lists Zeikus trace element solution with a "see Medium No. 684" attribute.

RECIPE_SOURCE https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=684 — JCM Medium No. 684 Trace mineral solution

JCM Medium No. 684 defines the pointed Trace mineral solution and enumerates nitrilotriacetic acid, Fe/Mn/Co/Ca/Zn/Cu salts, H3BO3, Na2MoO4 x 2 H2O, NaCl, Na2SeO3 x 5 H2O, and distilled water.

Occurrence statistics

Total occurrences: 1
Distinct media: 1

Curation history

Timestamp	Curator	Action	New status	Changes
2026-04-30T18:43:03.698454+00:00	audit_import_ingredients[mim-queue]	CREATED_AS_UNMAPPED	UNMAPPED	Imported from mim-queue (source_id=mediadive.ingredient:1914); no CAS-RN or CHEBI/NCIT match. Curator review needed.
2026-05-02T03:12:20.043652+00:00	auto_classify_ingredient_type	AUTO_CLASSIFY_INGREDIENT_TYPE		set ingredient_type=STOCK_SOLUTION (name matches solution pattern 'trace element')
2026-05-02T03:18:22.004700+00:00	resolve_unmapped_v2	AUTO_UPGRADE_TO_NCIT		primary UNMAPPED_0584 → NCIT:C896 (strategy=stem-match)
2026-08-27T03:16:39.520173+00:00	refresh_occurrence_statistics	CORRECTED		occurrence_statistics 0/0 -> 8/8 (media_count/total_occurrences), derived from distinct CultureMech recipe ids in the #337 occurrence table. The prior values came from the importer retired in #453, whose media_count counted a list truncated to 50 examples (#449). [source=ingredient_occurrences.tsv culturemech_rev=15a4da5c08 rows=194137 distinct_recipes=15257]
2026-09-06T00:00:00+00:00	mediaingredientmech-agentic-curation LLM	CORRECTED	MAPPED	Changed identifier from NCIT:C896 to kgmicrobe.ingredient:zeikus_trace_element_solution and retained NCIT:C896 only as CLOSE_MATCH. Zeikus trace element solution is a JCM/DSMZ stock formulation reference, not identical to the generic trace-element class.
2026-09-06T00:00:00+00:00	mediaingredientmech-agentic-curation LLM	ANNOTATED		Added the TRACE_ELEMENT role plus components and component_assertion by following MediaDive JCM medium J849's "see Medium No. 684" pointer to JCM medium 684, Trace mineral solution.
2026-09-10T02:58:59+00:00	split_trace_solution_memberships	CORRECTED		Corrected occurrence_statistics 8/8 -> 1/1 (media_count/total_occurrences) after source-label-specific CultureMech membership splitting in issue 560. The single mediadive.compound:1914 Zeikus trace element solution row from the 8c79a66fec occurrence snapshot now resolves to this local stock-solution identity instead of the parent NCIT:C896.