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8-azaguanine

CHEBI:63486 EXACT SEEDED cytotoxicenzyme inhibitor

What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

A triazolopyrimidine that consists of 3,6-dihydro-7H-[1,2,3]triazolo[4,5-d]pyrimidine bearing amino and oxo substituents at positions 5 and 7 respectively.

Structure

Standard InChIKeyLPXQRXLUHJKZIE-UHFFFAOYSA-N
SMILESC12=NNN=C1N=C(NC2=O)N
Stereochemistryfully defined
Cross-referencespubchem:135403646

Filing pathway

Alkaloids — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is other, from MIBiG.

Producer organisms 1

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

TaxonBasisClusterEvidence
Streptomyces pathocidini
NCBITaxon:1650571		 SOURCE_ASSERTION mibig:BGC0002508 PMID:32235841

Occurrences 0

None cited.

Biosynthetic gene clusters 1

AccessionHostLocus evidenceGenome
mibig:BGC0002508 Streptomyces pathocidini
NCBITaxon:1650571 		CLUSTER_DEMONSTRATED genbank:MT543149.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Mechanism & Biosynthesis Overview

PRODUCERS & OCCURRENCE 1 producer (0 causal) 0 cited occurrences BIOSYNTHETIC LOCUS 1 gene cluster 0 curated pathway steps NATURAL PRODUCT 8-azaguanine CHEBI:63486 Alkaloids TARGETS & BIOACTIVITY 0 molecular targets 25 bioactivities · 3 graphs

Causal graph — biosynthesis 10 nodes, 9 edges

Bounded genetic evidence for native-reference 8-AzgD/E/F in Streptomyces pathocidini CGMCC4.1633 (named S. albus subsp. pathocidicus in Hou et al.). The three deletion interventions and their product phenotypes are distinct from the encoded proteins. Main article and Figures 1/2 inspected; supplements and exact mutant constructs were not. CHEBI:63486 is the neutral free-base compound anchor, not an experimentally resolved solution tautomer or protonation state. No catalytic reaction, physical complex, universal necessity, other-strain Ptn equivalence, or producer/BGC evidence upgrade is asserted.

SubjectPredicateObjectNotesEvidence
CGMCC4.1633 8-azgD deletion deletes_gene_represented_by 8-AzgD (CGMCC4.1633 native reference) The identifier denotes the deposited native gene product, not an intact protein present in the deletion strain or a sequenced mutant allele. No equivalent UniProt reference or other-strain Ptn protein is substituted.
DOI:10.3389/fbioe.2020.603514
https://www.ncbi.nlm.nih.gov/protein/QLM04718.1
https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    The study names CGMCC4.1633 and deposits its 8-azg cluster as MT543149. Its in-frame deletion method reports double crossover and a six-base scar. Complete experimental mutant sequences and supplementary PCR data were not independently inspected.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QLM04718.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-10
    notes
    Independent GenPept QLM04718.1, complete 167 aa, 8-AzgD; source Streptomyces pathocidini CGMCC4.1633, taxon 1650571. coded_by complement(MT543149.1:3192..3695), translation table 11. The functional product-name annotations are not adopted as enzyme assays.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-10
    notes
    Complete 504-nt reverse-strand CDS 3192..3695, codon_start 1, table 11 and ATG initiation independently translate to all 167 residues of QLM04718.1, equal to the deposited CDS translation. Reference correspondence does not establish historical stock or mutant sequence identity. The article says Illumina, whereas the deposit assembly metadata says PacBio; that provenance discrepancy is retained, not adjudicated.
CGMCC4.1633 8-azgD deletion has_reported_trace_amount_of 8-azaguanine Figure 2A, trace v: trace product remains. This is not complete abolition, a numerical yield or a purified-enzyme reaction. Accumulated nucleoside congeners are not merged into the free-base owner.
DOI:10.3389/fbioe.2020.603514
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    The Results report trace 8-azaguanine in the individual D and E mutant broths; inspected Figure 2A distinguishes these outcomes from the F deletion.
    snippet
    only trace amount of 8-AZG
CGMCC4.1633 8-azgE deletion deletes_gene_represented_by 8-AzgE (CGMCC4.1633 native reference) The identifier denotes the deposited native gene product, not an intact protein present in the deletion strain or a sequenced mutant allele. No equivalent UniProt reference or other-strain Ptn protein is substituted.
DOI:10.3389/fbioe.2020.603514
https://www.ncbi.nlm.nih.gov/protein/QLM04711.1
https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    The study names CGMCC4.1633 and deposits its 8-azg cluster as MT543149. Its in-frame deletion method reports double crossover and a six-base scar. Complete experimental mutant sequences and supplementary PCR data were not independently inspected.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QLM04711.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-10
    notes
    Independent GenPept QLM04711.1, complete 102 aa, 8-AzgE; source Streptomyces pathocidini CGMCC4.1633, taxon 1650571. coded_by complement(MT543149.1:3702..4010), translation table 11. The functional product-name annotations are not adopted as enzyme assays.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-10
    notes
    Complete 309-nt reverse-strand CDS 3702..4010, codon_start 1, table 11 and GTG initiation independently translate to all 102 residues of QLM04711.1, equal to the deposited CDS translation. Reference correspondence does not establish historical stock or mutant sequence identity. The article says Illumina, whereas the deposit assembly metadata says PacBio; that provenance discrepancy is retained, not adjudicated.
CGMCC4.1633 8-azgE deletion has_reported_trace_amount_of 8-azaguanine Figure 2A, trace vi: trace product remains. This is not complete abolition, a numerical yield or a purified-enzyme reaction. Accumulated nucleoside congeners are not merged into the free-base owner.
DOI:10.3389/fbioe.2020.603514
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    The Results report trace 8-azaguanine in the individual D and E mutant broths; inspected Figure 2A distinguishes these outcomes from the F deletion.
CGMCC4.1633 8-azgF deletion deletes_gene_represented_by 8-AzgF (CGMCC4.1633 native reference) The identifier denotes the deposited native gene product, not an intact protein present in the deletion strain or a sequenced mutant allele. No equivalent UniProt reference or other-strain Ptn protein is substituted.
DOI:10.3389/fbioe.2020.603514
https://www.ncbi.nlm.nih.gov/protein/QLM04712.1
https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    The study names CGMCC4.1633 and deposits its 8-azg cluster as MT543149. Its in-frame deletion method reports double crossover and a six-base scar. Complete experimental mutant sequences and supplementary PCR data were not independently inspected.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QLM04712.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-10
    notes
    Independent GenPept QLM04712.1, complete 206 aa, 8-AzgF; source Streptomyces pathocidini CGMCC4.1633, taxon 1650571. coded_by complement(MT543149.1:4047..4667), translation table 11. The functional product-name annotations are not adopted as enzyme assays.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-10
    notes
    Complete 621-nt reverse-strand CDS 4047..4667, codon_start 1, table 11 and ATG initiation independently translate to all 206 residues of QLM04712.1, equal to the deposited CDS translation. Reference correspondence does not establish historical stock or mutant sequence identity. The article says Illumina, whereas the deposit assembly metadata says PacBio; that provenance discrepancy is retained, not adjudicated.
CGMCC4.1633 8-azgF deletion has_reported_loss_of_detectable 8-azaguanine Figure 2A, trace vii versus wild type i and authentic standard: reported loss of detectable 8-azaguanine under the study conditions. No numerical detection limit, absolute zero, general essentiality or directly measured GTP reaction is asserted.
DOI:10.3389/fbioe.2020.603514
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    Native-strain deletion phenotype from the Results and inspected Figure 2A; HPLC with UV detection at 245 nm, compared with an authentic 8-azaguanine standard.
    snippet
    8-AZG production was completely abolished
8-AzgD (CGMCC4.1633 native reference) has a sequence-identical UniProtKB example 8-AzgD (S. pathocidini ACH429_00980 UniProtKB example) UniProtKB:A0A6G9KJ81 is the active Streptomyces pathocidini (ACH429_00980) entry sharing 100% amino-acid sequence identity (UPI000B1A84C6) with MT543149.1 QLM04718.1 (8-AzgD).
https://www.uniprot.org/uniprotkb/A0A6G9KJ81
https://www.uniprot.org/uniparc/UPI000B1A84C6
https://www.ncbi.nlm.nih.gov/protein/QLM04718.1
  • reference
    https://www.uniprot.org/uniprotkb/A0A6G9KJ81
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    Active Streptomyces pathocidini UniProtKB entry (ACH429_00980) for the UPI000B1A84C6 sequence.
  • reference
    https://www.uniprot.org/uniparc/UPI000B1A84C6
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    UniParc UPI000B1A84C6 links MT543149.1 GenPept QLM04718.1 (8-AzgD) and active UniProtKB:A0A6G9KJ81 under a single 100%-identical sequence.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QLM04718.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    MT543149.1 GenPept QLM04718.1 is the deposited Streptomyces pathocidini CGMCC4.1633 8-AzgD translation.
8-AzgE (CGMCC4.1633 native reference) has a sequence-identical UniProtKB example 8-AzgE (S. pathocidini ACH429_00985 UniProtKB example) UniProtKB:A0A7G3ZQC3 is the active Streptomyces pathocidini (ACH429_00985) entry sharing 100% amino-acid sequence identity (UPI0006E3F696) with MT543149.1 QLM04711.1 (8-AzgE).
https://www.uniprot.org/uniprotkb/A0A7G3ZQC3
https://www.uniprot.org/uniparc/UPI0006E3F696
https://www.ncbi.nlm.nih.gov/protein/QLM04711.1
  • reference
    https://www.uniprot.org/uniprotkb/A0A7G3ZQC3
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    Active Streptomyces pathocidini UniProtKB entry (ACH429_00985) for the UPI0006E3F696 sequence.
  • reference
    https://www.uniprot.org/uniparc/UPI0006E3F696
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    UniParc UPI0006E3F696 links MT543149.1 GenPept QLM04711.1 (8-AzgE) and active UniProtKB:A0A7G3ZQC3 under a single 100%-identical sequence.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QLM04711.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    MT543149.1 GenPept QLM04711.1 is the deposited Streptomyces pathocidini CGMCC4.1633 8-AzgE translation.
8-AzgF (CGMCC4.1633 native reference) has a sequence-identical UniProtKB example 8-AzgF (S. pathocidini ACH429_00990 UniProtKB example) UniProtKB:A0A6G9KIY0 is the active Streptomyces pathocidini (ACH429_00990) entry sharing 100% amino-acid sequence identity (UPI00099E5E15) with MT543149.1 QLM04712.1 (8-AzgF).
https://www.uniprot.org/uniprotkb/A0A6G9KIY0
https://www.uniprot.org/uniparc/UPI00099E5E15
https://www.ncbi.nlm.nih.gov/protein/QLM04712.1
  • reference
    https://www.uniprot.org/uniprotkb/A0A6G9KIY0
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    Active Streptomyces pathocidini UniProtKB entry (ACH429_00990) for the UPI00099E5E15 sequence.
  • reference
    https://www.uniprot.org/uniparc/UPI00099E5E15
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    UniParc UPI00099E5E15 links MT543149.1 GenPept QLM04712.1 (8-AzgF) and active UniProtKB:A0A6G9KIY0 under a single 100%-identical sequence.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QLM04712.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    MT543149.1 GenPept QLM04712.1 is the deposited Streptomyces pathocidini CGMCC4.1633 8-AzgF translation.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 9 nodes, 8 edges

Bounded native genetic evidence for 8-AzgA/B/G in Streptomyces pathocidini CGMCC4.1633, named S. albus subsp. pathocidicus in Hou et al. Deletion interventions are separate from deposited protein references. The previously archived main text and Figures 1/2 were inspected; supplements and exact mutant constructs remain uninspected. This graph records reduced-product phenotypes, not A nitric-oxide enzyme activity, B nitrogen-species transport, G radical chemistry, physical assembly or other-strain Ptn equivalence. CHEBI:63486 anchors the neutral free base, not a resolved solution microspecies. Existing D/E/F evidence and all source-owned claims are retained separately.

SubjectPredicateObjectNotesEvidence
CGMCC4.1633 8-azgA deletion deletes_gene_represented_by 8-AzgA (CGMCC4.1633 native reference) The identifier denotes a deposited native gene product, not an intact protein in the deletion strain or an independently sequenced mutant allele. No UniProt homolog or other-strain Ptn protein is substituted.
DOI:10.3389/fbioe.2020.603514
https://www.ncbi.nlm.nih.gov/protein/QLM04708.1
https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    The study identifies the CGMCC4.1633 cluster as MT543149 and reports in-frame, double-crossover gene deletions leaving a six-base scar. Exact allele sequences, supplementary PCR evidence and polar effects were not independently resolved.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QLM04708.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-10
    notes
    Independent GenPept QLM04708.1: 8-AzgA, complete 390 aa; Streptomyces pathocidini CGMCC4.1633, taxon 1650571. coded_by complement(MT543149.1:1..1173), table 11. Functional and domain annotations are not adopted as enzyme or transport assays.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-10
    notes
    The complete 1173-nt reverse-strand CDS 1..1173, codon_start 1, table 11 and GTG initiation independently translate to all 390 residues of QLM04708.1, matching the deposited translation. This is reference correspondence, not historical-stock or mutant-sequence identity. Article Illumina versus deposit PacBio metadata remains unresolved.
CGMCC4.1633 8-azgA deletion has_reported_reduced_amount_of 8-azaguanine Figure 2A trace iii, compared with wild type i and authentic standard viiii. The Results report lower product under these culture conditions, not abolition. No percentage, absolute yield, statistical significance, universal necessity or direct catalytic activity is inferred from peak height.
DOI:10.3389/fbioe.2020.603514
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    Native-strain deletion phenotype in the Results and inspected Figure 2A; HPLC/UV at 245 nm. The panel has no quantitative vertical scale or integrated yield values. Lower titer is the authors' report, not an independent numerical estimate from the plotted traces.
    snippet
    titer of 8-AZG correspondingly decreased
CGMCC4.1633 8-azgB deletion deletes_gene_represented_by 8-AzgB (CGMCC4.1633 native reference) The identifier denotes a deposited native gene product, not an intact protein in the deletion strain or an independently sequenced mutant allele. UniParc UPI0006E298C9 links inactive S. pathocidini TrEMBL A0A6G9KGR0; no homolog substituted.
DOI:10.3389/fbioe.2020.603514
https://www.ncbi.nlm.nih.gov/protein/QLM04709.1
https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
https://rest.uniprot.org/uniparc/UPI0006E298C9.json
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    The study identifies the CGMCC4.1633 cluster as MT543149 and reports in-frame, double-crossover gene deletions leaving a six-base scar. Exact allele sequences, supplementary PCR evidence and polar effects were not independently resolved.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QLM04709.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-10
    notes
    Independent GenPept QLM04709.1: 8-AzgB, complete 408 aa; Streptomyces pathocidini CGMCC4.1633, taxon 1650571. coded_by complement(MT543149.1:1170..2396), table 11. Functional and domain annotations are not adopted as enzyme or transport assays.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-10
    notes
    The complete 1227-nt reverse-strand CDS 1170..2396, codon_start 1, table 11 and ATG initiation independently translate to all 408 residues of QLM04709.1, matching the deposited translation. This is reference correspondence, not historical-stock or mutant-sequence identity. Article Illumina versus deposit PacBio metadata remains unresolved.
  • reference
    https://rest.uniprot.org/uniparc/UPI0006E298C9.json
    evidence type
    DATABASE_ASSERTION
    notes
    UPI0006E298C9 (408 aa) links active EMBL QLM04709.1 and inactive S. pathocidini TrEMBL A0A6G9KGR0 (ACH429_00970); active UniProtKB xrefs are other Streptomyces spp.
    retrieved on
    2026-10-30
CGMCC4.1633 8-azgB deletion has_reported_reduced_amount_of 8-azaguanine Figure 2A trace iv, compared with wild type i and authentic standard viiii. The Results report lower product under these culture conditions, not abolition. No percentage, absolute yield, statistical significance, universal necessity or direct catalytic activity is inferred from peak height.
DOI:10.3389/fbioe.2020.603514
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    Native-strain deletion phenotype in the Results and inspected Figure 2A; HPLC/UV at 245 nm. The panel has no quantitative vertical scale or integrated yield values. Lower titer is the authors' report, not an independent numerical estimate from the plotted traces.
CGMCC4.1633 8-azgG deletion deletes_gene_represented_by 8-AzgG (CGMCC4.1633 native reference) The identifier denotes a deposited native gene product, not an intact protein in the deletion strain or an independently sequenced mutant allele. No UniProt homolog or other-strain Ptn protein is substituted.
DOI:10.3389/fbioe.2020.603514
https://www.ncbi.nlm.nih.gov/protein/QLM04713.1
https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    The study identifies the CGMCC4.1633 cluster as MT543149 and reports in-frame, double-crossover gene deletions leaving a six-base scar. Exact allele sequences, supplementary PCR evidence and polar effects were not independently resolved.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QLM04713.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-10
    notes
    Independent GenPept QLM04713.1: 8-AzgG, complete 462 aa; Streptomyces pathocidini CGMCC4.1633, taxon 1650571. coded_by complement(MT543149.1:4903..6291), table 11. Functional and domain annotations are not adopted as enzyme or transport assays.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-10
    notes
    The complete 1389-nt reverse-strand CDS 4903..6291, codon_start 1, table 11 and ATG initiation independently translate to all 462 residues of QLM04713.1, matching the deposited translation. This is reference correspondence, not historical-stock or mutant-sequence identity. Article Illumina versus deposit PacBio metadata remains unresolved.
CGMCC4.1633 8-azgG deletion has_reported_reduced_amount_of 8-azaguanine Figure 2A trace viii, compared with wild type i and authentic standard viiii. The Results report lower product under these culture conditions, not abolition. No percentage, absolute yield, statistical significance, universal necessity or direct catalytic activity is inferred from peak height.
DOI:10.3389/fbioe.2020.603514
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    Native-strain deletion phenotype in the Results and inspected Figure 2A; HPLC/UV at 245 nm. The panel has no quantitative vertical scale or integrated yield values. Lower titer is the authors' report, not an independent numerical estimate from the plotted traces.
8-AzgA (CGMCC4.1633 native reference) has a sequence-identical UniProtKB example 8-AzgA (S. pathocidini ACH429_00965 UniProtKB example) UniProtKB:A0A6G9KI63 is the active Streptomyces pathocidini (ACH429_00965) entry sharing 100% amino-acid sequence identity (UPI000A88606E) with MT543149.1 QLM04708.1 (8-AzgA).
https://www.uniprot.org/uniprotkb/A0A6G9KI63
https://www.uniprot.org/uniparc/UPI000A88606E
https://www.ncbi.nlm.nih.gov/protein/QLM04708.1
  • reference
    https://www.uniprot.org/uniprotkb/A0A6G9KI63
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    Active Streptomyces pathocidini UniProtKB entry (ACH429_00965) for the UPI000A88606E sequence.
  • reference
    https://www.uniprot.org/uniparc/UPI000A88606E
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    UniParc UPI000A88606E links MT543149.1 GenPept QLM04708.1 (8-AzgA) and active UniProtKB:A0A6G9KI63 under a single 100%-identical sequence.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QLM04708.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    MT543149.1 GenPept QLM04708.1 is the deposited Streptomyces pathocidini CGMCC4.1633 8-AzgA translation.
8-AzgG (CGMCC4.1633 native reference) has a sequence-identical UniProtKB example 8-AzgG (S. pathocidini ACH429_00995 UniProtKB example) UniProtKB:A0A7G3ZQC5 is the active Streptomyces pathocidini (ACH429_00995) entry sharing 100% amino-acid sequence identity (UPI0006E15438) with MT543149.1 QLM04713.1 (8-AzgG).
https://www.uniprot.org/uniprotkb/A0A7G3ZQC5
https://www.uniprot.org/uniparc/UPI0006E15438
https://www.ncbi.nlm.nih.gov/protein/QLM04713.1
  • reference
    https://www.uniprot.org/uniprotkb/A0A7G3ZQC5
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    Active Streptomyces pathocidini UniProtKB entry (ACH429_00995) for the UPI0006E15438 sequence.
  • reference
    https://www.uniprot.org/uniparc/UPI0006E15438
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    UniParc UPI0006E15438 links MT543149.1 GenPept QLM04713.1 (8-AzgG) and active UniProtKB:A0A7G3ZQC5 under a single 100%-identical sequence.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QLM04713.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    MT543149.1 GenPept QLM04713.1 is the deposited Streptomyces pathocidini CGMCC4.1633 8-AzgG translation.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 13 nodes, 12 edges

Reported native genetic-test outcomes for 8-AzgC/H/I/J/K in Streptomyces pathocidini CGMCC4.1633, named S. albus subsp. pathocidicus in Hou et al. C/H/J deletions have reported reduced product, K a small increase, and I no reported change. I is included to preserve the negative experimental context, not to assign a positive biosynthetic function. Deletion interventions are distinct from deposited protein references. Evidence is the archived main-text Methods/Results; supplementary Figure 4 and PCR data remain uninspected. No catalytic reaction, transcriptional regulation, physical assembly, general essentiality/dispensability, J1074 equivalence or producer/BGC upgrade is asserted. CHEBI:63486 denotes the neutral free base, not a resolved solution tautomer or protonation state.

SubjectPredicateObjectNotesEvidence
CGMCC4.1633 8-azgC deletion deletes_gene_represented_by 8-AzgC (CGMCC4.1633 native reference) The accession denotes the deposited native gene product, not an intact protein in the deletion strain or an independently sequenced mutant allele. No exact UniProt mapping was established by the archived identifier-specific query; no homolog or other-strain Ptn protein is substituted.
DOI:10.3389/fbioe.2020.603514
https://www.ncbi.nlm.nih.gov/protein/QLM04710.1
https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    The Methods explicitly list this individual knockout among the native CGMCC4.1633 interventions using pKC1139 and S17-1 conjugation, separate from J1074 heterologous expression. The Results report in-frame double crossovers with six-base scars. Supplementary PCR evidence, complete mutant sequences and possible polar effects were not independently verified.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QLM04710.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-10
    notes
    Independent GenPept QLM04710.1: 8-AzgC, complete 236 aa; Streptomyces pathocidini CGMCC4.1633, taxon 1650571. coded_by complement(MT543149.1:2417..3127), table 11. Product-name and domain annotations are not adopted as biochemical assays.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-10
    notes
    The complete 711-nt reverse-strand CDS 2417..3127, codon_start 1, table 11 and ATG initiation independently translate to all 236 residues of QLM04710.1, matching the deposited translation. Reference correspondence is not historical-stock or mutant-sequence identity. Article Illumina versus deposit PacBio metadata remains unresolved.
CGMCC4.1633 8-azgC deletion has_reported_reduced_amount_of 8-azaguanine The Results report lower 8-azaguanine in this individual mutant fermentation broth relative to wild type. This is a qualitative main-text report, not abolition, a quantified yield, statistical significance or a catalytic assignment.
DOI:10.3389/fbioe.2020.603514
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    Native-knockout Methods and Results main text were inspected. The article cites supplementary Figure 4 for these phenotypes; that figure and supplementary PCR/allele data were not inspected. The general production-analysis method uses HPLC/UV at 245 nm with authentic 8-azaguanine. No independent peak integration, replicate assessment or quantitative phenotype estimate was performed.
    snippet
    correspondingly decreased
CGMCC4.1633 8-azgH deletion deletes_gene_represented_by 8-AzgH (CGMCC4.1633 native reference) The accession denotes the deposited native gene product, not an intact protein in the deletion strain or an independently sequenced mutant allele. No exact UniProt mapping was established by the archived identifier-specific query; no homolog or other-strain Ptn protein is substituted.
DOI:10.3389/fbioe.2020.603514
https://www.ncbi.nlm.nih.gov/protein/QLM04714.1
https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    The Methods explicitly list this individual knockout among the native CGMCC4.1633 interventions using pKC1139 and S17-1 conjugation, separate from J1074 heterologous expression. The Results report in-frame double crossovers with six-base scars. Supplementary PCR evidence, complete mutant sequences and possible polar effects were not independently verified.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QLM04714.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-10
    notes
    Independent GenPept QLM04714.1: 8-AzgH, complete 378 aa; Streptomyces pathocidini CGMCC4.1633, taxon 1650571. coded_by MT543149.1:6461..7597, table 11. Product-name and domain annotations are not adopted as biochemical assays.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-10
    notes
    The complete 1137-nt forward-strand CDS 6461..7597, codon_start 1, table 11 and TTG initiation independently translate to all 378 residues of QLM04714.1, matching the deposited translation. Reference correspondence is not historical-stock or mutant-sequence identity. Article Illumina versus deposit PacBio metadata remains unresolved.
CGMCC4.1633 8-azgH deletion has_reported_reduced_amount_of 8-azaguanine The Results report lower 8-azaguanine in this individual mutant fermentation broth relative to wild type. This is a qualitative main-text report, not abolition, a quantified yield, statistical significance or a catalytic assignment.
DOI:10.3389/fbioe.2020.603514
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    Native-knockout Methods and Results main text were inspected. The article cites supplementary Figure 4 for these phenotypes; that figure and supplementary PCR/allele data were not inspected. The general production-analysis method uses HPLC/UV at 245 nm with authentic 8-azaguanine. No independent peak integration, replicate assessment or quantitative phenotype estimate was performed.
CGMCC4.1633 8-azgI deletion deletes_gene_represented_by 8-AzgI (CGMCC4.1633 native reference) The accession denotes the deposited native gene product, not an intact protein in the deletion strain or an independently sequenced mutant allele. UniParc UPI001862886B links inactive S. pathocidini TrEMBL A0A7G3ZQC7 (zero active UniProtKB xrefs); no homolog substituted.
DOI:10.3389/fbioe.2020.603514
https://www.ncbi.nlm.nih.gov/protein/QLM04715.1
https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
https://rest.uniprot.org/uniparc/UPI001862886B.json
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    The Methods explicitly list this individual knockout among the native CGMCC4.1633 interventions using pKC1139 and S17-1 conjugation, separate from J1074 heterologous expression. The Results report in-frame double crossovers with six-base scars. Supplementary PCR evidence, complete mutant sequences and possible polar effects were not independently verified.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QLM04715.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-10
    notes
    Independent GenPept QLM04715.1: 8-AzgI, complete 682 aa; Streptomyces pathocidini CGMCC4.1633, taxon 1650571. coded_by MT543149.1:7863..9911, table 11. Product-name and domain annotations are not adopted as biochemical assays.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-10
    notes
    The complete 2049-nt forward-strand CDS 7863..9911, codon_start 1, table 11 and TTG initiation independently translate to all 682 residues of QLM04715.1, matching the deposited translation. Reference correspondence is not historical-stock or mutant-sequence identity. Article Illumina versus deposit PacBio metadata remains unresolved.
  • reference
    https://rest.uniprot.org/uniparc/UPI001862886B.json
    evidence type
    DATABASE_ASSERTION
    notes
    UniParc UPI001862886B (682 aa) links active EMBL QLM04715.1 (8-AzgI) and inactive S. pathocidini TrEMBL A0A7G3ZQC7 (zero active UniProtKB xrefs).
    retrieved on
    2026-10-30
CGMCC4.1633 8-azgI deletion has_reported_no_change_in_amount_of 8-azaguanine The Results report no effect on 8-azaguanine production after this deletion under the tested conditions. Included as an explicit negative genetic-test context, not a positive biosynthetic role or causal effect. No equivalence margin, detection power, universal dispensability or biochemical function is established by this report.
DOI:10.3389/fbioe.2020.603514
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    Native-knockout Methods and Results main text were inspected. The article cites supplementary Figure 4 for these phenotypes; that figure and supplementary PCR/allele data were not inspected. The general production-analysis method uses HPLC/UV at 245 nm with authentic 8-azaguanine. No independent peak integration, replicate assessment or quantitative phenotype estimate was performed.
    snippet
    had no effect
CGMCC4.1633 8-azgJ deletion deletes_gene_represented_by 8-AzgJ (CGMCC4.1633 native reference) The accession denotes the deposited native gene product, not an intact protein in the deletion strain or an independently sequenced mutant allele. UniParc UPI00185FCABD links inactive S. pathocidini TrEMBL A0A7G3ZQC8 (zero active UniProtKB xrefs); no homolog substituted.
DOI:10.3389/fbioe.2020.603514
https://www.ncbi.nlm.nih.gov/protein/QLM04716.1
https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
https://rest.uniprot.org/uniparc/UPI00185FCABD.json
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    The Methods explicitly list this individual knockout among the native CGMCC4.1633 interventions using pKC1139 and S17-1 conjugation, separate from J1074 heterologous expression. The Results report in-frame double crossovers with six-base scars. Supplementary PCR evidence, complete mutant sequences and possible polar effects were not independently verified.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QLM04716.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-10
    notes
    Independent GenPept QLM04716.1: 8-AzgJ, complete 103 aa; Streptomyces pathocidini CGMCC4.1633, taxon 1650571. coded_by MT543149.1:10045..10356, table 11. Product-name and domain annotations are not adopted as biochemical assays.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-10
    notes
    The complete 312-nt forward-strand CDS 10045..10356, codon_start 1, table 11 and GTG initiation independently translate to all 103 residues of QLM04716.1, matching the deposited translation. Reference correspondence is not historical-stock or mutant-sequence identity. Article Illumina versus deposit PacBio metadata remains unresolved.
  • reference
    https://rest.uniprot.org/uniparc/UPI00185FCABD.json
    evidence type
    DATABASE_ASSERTION
    notes
    UniParc UPI00185FCABD (103 aa) links active EMBL QLM04716.1 (8-AzgJ) and inactive S. pathocidini TrEMBL A0A7G3ZQC8 (zero active UniProtKB xrefs).
    retrieved on
    2026-10-30
CGMCC4.1633 8-azgJ deletion has_reported_reduced_amount_of 8-azaguanine The Results report lower 8-azaguanine in this individual mutant fermentation broth relative to wild type. This is a qualitative main-text report, not abolition, a quantified yield, statistical significance or a catalytic assignment.
DOI:10.3389/fbioe.2020.603514
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    Native-knockout Methods and Results main text were inspected. The article cites supplementary Figure 4 for these phenotypes; that figure and supplementary PCR/allele data were not inspected. The general production-analysis method uses HPLC/UV at 245 nm with authentic 8-azaguanine. No independent peak integration, replicate assessment or quantitative phenotype estimate was performed.
CGMCC4.1633 8-azgK deletion deletes_gene_represented_by 8-AzgK (CGMCC4.1633 native reference) The accession denotes the deposited native gene product, not an intact protein in the deletion strain or an independently sequenced mutant allele. UniParc UPI0006E35D5C links inactive S. pathocidini TrEMBL A0A7G3ZQC9 (ACH429_01015) (active UniProtKB xrefs are other Streptomyces spp.); no homolog substituted.
DOI:10.3389/fbioe.2020.603514
https://www.ncbi.nlm.nih.gov/protein/QLM04717.1
https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
https://rest.uniprot.org/uniparc/UPI0006E35D5C.json
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    The Methods explicitly list this individual knockout among the native CGMCC4.1633 interventions using pKC1139 and S17-1 conjugation, separate from J1074 heterologous expression. The Results report in-frame double crossovers with six-base scars. Supplementary PCR evidence, complete mutant sequences and possible polar effects were not independently verified.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QLM04717.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-10
    notes
    Independent GenPept QLM04717.1: 8-AzgK, complete 277 aa; Streptomyces pathocidini CGMCC4.1633, taxon 1650571. coded_by MT543149.1:10590..11423, table 11. Product-name and domain annotations are not adopted as biochemical assays.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/MT543149.1
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-10
    notes
    The complete 834-nt forward-strand CDS 10590..11423, codon_start 1, table 11 and ATG initiation independently translate to all 277 residues of QLM04717.1, matching the deposited translation. Reference correspondence is not historical-stock or mutant-sequence identity. Article Illumina versus deposit PacBio metadata remains unresolved.
  • reference
    https://rest.uniprot.org/uniparc/UPI0006E35D5C.json
    evidence type
    DATABASE_ASSERTION
    notes
    UniParc UPI0006E35D5C (277 aa) links active EMBL QLM04717.1 (8-AzgK) and inactive S. pathocidini TrEMBL A0A7G3ZQC9 (ACH429_01015) (active UniProtKB xrefs are other Streptomyces spp.).
    retrieved on
    2026-10-30
CGMCC4.1633 8-azgK deletion has_reported_small_increase_in_amount_of 8-azaguanine The Results report a small increase in 8-azaguanine titer after this deletion. The article interprets K as a negative transcriptional regulator, but the genetic phenotype alone does not establish that mechanism; it is not adopted here. The deposited glycosyltransferase annotation is not an enzyme assay. No numerical increase or statistical significance is asserted.
DOI:10.3389/fbioe.2020.603514
  • reference
    DOI:10.3389/fbioe.2020.603514
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-10
    notes
    Native-knockout Methods and Results main text were inspected. The article cites supplementary Figure 4 for these phenotypes; that figure and supplementary PCR/allele data were not inspected. The general production-analysis method uses HPLC/UV at 245 nm with authentic 8-azaguanine. No independent peak integration, replicate assessment or quantitative phenotype estimate was performed.
    snippet
    a small titer increase
8-AzgC (CGMCC4.1633 native reference) has a sequence-identical UniProtKB example 8-AzgC (S. pathocidini ACH429_00975 UniProtKB example) UniProtKB:A0A6G9KI21 is the active Streptomyces pathocidini (ACH429_00975) entry sharing 100% amino-acid sequence identity (UPI0006E1F3FA) with MT543149.1 QLM04710.1 (8-AzgC).
https://www.uniprot.org/uniprotkb/A0A6G9KI21
https://www.uniprot.org/uniparc/UPI0006E1F3FA
https://www.ncbi.nlm.nih.gov/protein/QLM04710.1
  • reference
    https://www.uniprot.org/uniprotkb/A0A6G9KI21
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    Active Streptomyces pathocidini UniProtKB entry (ACH429_00975) for the UPI0006E1F3FA sequence.
  • reference
    https://www.uniprot.org/uniparc/UPI0006E1F3FA
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    UniParc UPI0006E1F3FA links MT543149.1 GenPept QLM04710.1 (8-AzgC) and active UniProtKB:A0A6G9KI21 under a single 100%-identical sequence.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QLM04710.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    MT543149.1 GenPept QLM04710.1 is the deposited Streptomyces pathocidini CGMCC4.1633 8-AzgC translation.
8-AzgH (CGMCC4.1633 native reference) has a sequence-identical UniProtKB example 8-AzgH (S. pathocidini ACH429_01000 UniProtKB example) UniProtKB:A0A7G3ZQC6 is the active Streptomyces pathocidini (ACH429_01000) entry sharing 100% amino-acid sequence identity (UPI000B2DAD11) with MT543149.1 QLM04714.1 (8-AzgH).
https://www.uniprot.org/uniprotkb/A0A7G3ZQC6
https://www.uniprot.org/uniparc/UPI000B2DAD11
https://www.ncbi.nlm.nih.gov/protein/QLM04714.1
  • reference
    https://www.uniprot.org/uniprotkb/A0A7G3ZQC6
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    Active Streptomyces pathocidini UniProtKB entry (ACH429_01000) for the UPI000B2DAD11 sequence.
  • reference
    https://www.uniprot.org/uniparc/UPI000B2DAD11
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    UniParc UPI000B2DAD11 links MT543149.1 GenPept QLM04714.1 (8-AzgH) and active UniProtKB:A0A7G3ZQC6 under a single 100%-identical sequence.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/QLM04714.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-17
    notes
    MT543149.1 GenPept QLM04714.1 is the deposited Streptomyces pathocidini CGMCC4.1633 8-AzgH translation.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Bioactivities 25

Entries preserve source qualifiers, targets and evidence. Repeated assay names or values are not independent replications; inspect source detail for differences.

AssayResultTarget enzymeSource detail
A quantitative high throughput screen for small molecules that induce DNA re-replication in MCF 10a normal breast cells.OTHER 0.2909 uM; NOT_DETERMINEDNot recorded
pubchem.aid:624296
assay
A quantitative high throughput screen for small molecules that induce DNA re-replication in MCF 10a normal breast cells.
evidence
  • reference
    pubchem.aid:624296
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 624296, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. Target accession NP_056979 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
NOT_DETERMINED
measurement type
OTHER
value
0.2909
units
uM
A quantitative high throughput screen for small molecules that induce DNA re-replication in SW480 colon adenocarcinoma cells.OTHER 14.581 uM; NOT_DETERMINEDNot recorded
pubchem.aid:624297
assay
A quantitative high throughput screen for small molecules that induce DNA re-replication in SW480 colon adenocarcinoma cells.
evidence
  • reference
    pubchem.aid:624297
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 624297, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. Target accession NP_056979 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
NOT_DETERMINED
measurement type
OTHER
value
14.581
units
uM
Confirmation and Secondary Assays for Modulators of Hemoglobin Beta Chain Splicing at IVS2 654 and 705 lociOTHER 2.51189 uM; ACTIVENot recorded
pubchem.aid:1405
assay
Confirmation and Secondary Assays for Modulators of Hemoglobin Beta Chain Splicing at IVS2 654 and 705 loci
evidence
  • reference
    pubchem.aid:1405
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1405, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. Target accession NP_000509 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
ACTIVE
measurement type
OTHER
value
2.51189
units
uM
Counterscreen for activators of the function of SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (SMARCA2, BRM): Luminescence-based cell-based high throughput dose response assay to identify non-selective compounds using the VP16 reporter assayEC50 91.17 uM; INACTIVENot recorded
pubchem.aid:686995
assay
Counterscreen for activators of the function of SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (SMARCA2, BRM): Luminescence-based cell-based high throughput dose response assay to identify non-selective compounds using the VP16 reporter assay
evidence
  • reference
    pubchem.aid:686995
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 686995, deposited by The Scripps Research Institute Molecular Screening Center; assay type Confirmatory.
call
INACTIVE
measurement type
EC50
value
91.17
units
uM
Dose Response selectivity of uHTS chemical inhibitors of T-cell specific antigen receptor-induced NF-kB activation in a 697B cell line using a luminescence assayIC50 2.56 uM; ACTIVENot recorded
pubchem.aid:489033
assay
Dose Response selectivity of uHTS chemical inhibitors of T-cell specific antigen receptor-induced NF-kB activation in a 697B cell line using a luminescence assay
evidence
  • reference
    pubchem.aid:489033
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 489033, deposited by Burnham Center for Chemical Genomics; assay type Confirmatory.
call
ACTIVE
measurement type
IC50
value
2.56
units
uM
activity class
ENZYME_INHIBITOR
Dose response confirmation of uHTS chemical inhibitors of T-cell specific antigen receptor-induced NF-kB activation in a Jurkat cell line using a luminescence assayIC50 80 uM; INACTIVENot recorded
pubchem.aid:489035
assay
Dose response confirmation of uHTS chemical inhibitors of T-cell specific antigen receptor-induced NF-kB activation in a Jurkat cell line using a luminescence assay
evidence
  • reference
    pubchem.aid:489035
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 489035, deposited by Burnham Center for Chemical Genomics; assay type Confirmatory.
call
INACTIVE
measurement type
IC50
value
80.0
units
uM
Dose response counterscreen of uHTS chemical inhibitors of T-cell specific antigen receptor-induced NF-kB activation in a HEK-293T cell line using a luminescence assayIC50 80 uM; INACTIVENot recorded
pubchem.aid:489041
assay
Dose response counterscreen of uHTS chemical inhibitors of T-cell specific antigen receptor-induced NF-kB activation in a HEK-293T cell line using a luminescence assay
evidence
  • reference
    pubchem.aid:489041
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 489041, deposited by Burnham Center for Chemical Genomics; assay type Confirmatory.
call
INACTIVE
measurement type
IC50
value
80.0
units
uM
Dose response cytotoxicity of uHTS chemical inhibitors of T-cell specific antigen receptor-induced NF-kB activation in a HEK-293T cell line using a luminescence assayIC50 80 uM; INACTIVENot recorded
pubchem.aid:489026
assay
Dose response cytotoxicity of uHTS chemical inhibitors of T-cell specific antigen receptor-induced NF-kB activation in a HEK-293T cell line using a luminescence assay
evidence
  • reference
    pubchem.aid:489026
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 489026, deposited by Burnham Center for Chemical Genomics; assay type Confirmatory.
call
INACTIVE
measurement type
IC50
value
80.0
units
uM
Luminescence Cell-Based Dose Response HTS Screen to Identify Cytotoxic Compounds of NIH3T3 cells.EC50 10.345 uM; ACTIVENot recorded
pubchem.aid:2010
assay
Luminescence Cell-Based Dose Response HTS Screen to Identify Cytotoxic Compounds of NIH3T3 cells.
evidence
  • reference
    pubchem.aid:2010
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2010, deposited by Broad Institute; assay type Confirmatory.
call
ACTIVE
measurement type
EC50
value
10.345
units
uM
activity class
CYTOTOXIC
Luminescence Cell-Based/Microorganism Dose Confirmation HTS to Identify Inhibitors of T.Cruzi Replication.EC50 0.1 uM; ACTIVENot recorded
pubchem.aid:2044
assay
Luminescence Cell-Based/Microorganism Dose Confirmation HTS to Identify Inhibitors of T.Cruzi Replication.
evidence
  • reference
    pubchem.aid:2044
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2044, deposited by Broad Institute; assay type Confirmatory.
call
ACTIVE
measurement type
EC50
value
0.1
units
uM
activity class
ENZYME_INHIBITOR
Luminescence Cell-Based/Microorganism Primary HTS to Identify Inhibitors of T.Cruzi InfectionEC50 0.153 uM; ACTIVENot recorded
pubchem.aid:2294
assay
Luminescence Cell-Based/Microorganism Primary HTS to Identify Inhibitors of T.Cruzi Infection
evidence
  • reference
    pubchem.aid:2294
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2294, deposited by Broad Institute; assay type Confirmatory.
call
ACTIVE
measurement type
EC50
value
0.153
units
uM
activity class
ENZYME_INHIBITOR
Luminescence-based cell-based high throughput dose response assay to identify activators of the function of SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (SMARCA2, BRM)EC50 91.17 uM; INACTIVENot recorded
pubchem.aid:686991
assay
Luminescence-based cell-based high throughput dose response assay to identify activators of the function of SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (SMARCA2, BRM)
evidence
  • reference
    pubchem.aid:686991
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 686991, deposited by The Scripps Research Institute Molecular Screening Center; assay type Confirmatory. Target accession EAW58811 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
INACTIVE
measurement type
EC50
value
91.17
units
uM
MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_DoseNoFile_CherryPick_Activity_Set2OTHER 0.875 uM; ACTIVENot recorded
pubchem.aid:493177
assay
MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_DoseNoFile_CherryPick_Activity_Set2
evidence
  • reference
    pubchem.aid:493177
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 493177, deposited by Broad Institute; assay type Confirmatory. Source endpoint: AC50. Target accession AAH65243 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
ACTIVE
measurement type
OTHER
value
0.875
units
uM
MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_DoseNoFile_CherryPick_Activity_Set3OTHER 0.875 uM; ACTIVENot recorded
pubchem.aid:493073
assay
MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_DoseNoFile_CherryPick_Activity_Set3
evidence
  • reference
    pubchem.aid:493073
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 493073, deposited by Broad Institute; assay type Confirmatory. Source endpoint: AC50. Target accession AAH65243 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
ACTIVE
measurement type
OTHER
value
0.875
units
uM
MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_DoseNoFile_CherryPick_Activity_Set4OTHER 0.875 uM; ACTIVENot recorded
pubchem.aid:493102
assay
MITF Measured in Cell-Based System Using Plate Reader - 2084-01_Inhibitor_DoseNoFile_CherryPick_Activity_Set4
evidence
  • reference
    pubchem.aid:493102
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 493102, deposited by Broad Institute; assay type Confirmatory. Source endpoint: AC50. Target accession AAH65243 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
ACTIVE
measurement type
OTHER
value
0.875
units
uM
Nrf2 qHTS screen for inhibitorsOTHER 29.0929 uM; NOT_DETERMINEDNot recorded
pubchem.aid:504444
assay
Nrf2 qHTS screen for inhibitors
evidence
  • reference
    pubchem.aid:504444
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 504444, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. Target accession NP_001138884 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
NOT_DETERMINED
measurement type
OTHER
value
29.0929
units
uM
Total Fluorescence Counterscreen for Inhibitors of the Interaction of Thyroid Hormone Receptor and Steroid Receptor Coregulator 2OTHER 79.4328 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1479
assay
Total Fluorescence Counterscreen for Inhibitors of the Interaction of Thyroid Hormone Receptor and Steroid Receptor Coregulator 2
evidence
  • reference
    pubchem.aid:1479
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1479, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. Target accession NP_001121649 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
NOT_DETERMINED
measurement type
OTHER
value
79.4328
units
uM
Validation qHTS for agonist of cAMP-regulated guanine nucleotide exchange factor 3 (EPAC1)OTHER 11.2202 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1645883
assay
Validation qHTS for agonist of cAMP-regulated guanine nucleotide exchange factor 3 (EPAC1)
evidence
  • reference
    pubchem.aid:1645883
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1645883, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
11.2202
units
uM
Validation qHTS for agonist of cAMP-regulated guanine nucleotide exchange factor 3 (EPAC1)OTHER 11.2202 uM; NOT_DETERMINEDUniProtKB:O95398
pubchem.aid:1645883
assay
Validation qHTS for agonist of cAMP-regulated guanine nucleotide exchange factor 3 (EPAC1)
evidence
  • reference
    pubchem.aid:1645883
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1645883, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
11.2202
units
uM
target enzyme
UniProtKB:O95398
Validation qHTS for agonist of cAMP-regulated guanine nucleotide exchange factor 3 (EPAC1)OTHER 125.892 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1645883
assay
Validation qHTS for agonist of cAMP-regulated guanine nucleotide exchange factor 3 (EPAC1)
evidence
  • reference
    pubchem.aid:1645883
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1645883, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
125.892
units
uM
Validation qHTS for agonist of cAMP-regulated guanine nucleotide exchange factor 3 (EPAC1)OTHER 125.892 uM; NOT_DETERMINEDUniProtKB:O95398
pubchem.aid:1645883
assay
Validation qHTS for agonist of cAMP-regulated guanine nucleotide exchange factor 3 (EPAC1)
evidence
  • reference
    pubchem.aid:1645883
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1645883, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
125.892
units
uM
target enzyme
UniProtKB:O95398
Validation qHTS for antagonist of cAMP-regulated guanine nucleotide exchange factor 4 (EPAC2)OTHER 0.4467 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1645886
assay
Validation qHTS for antagonist of cAMP-regulated guanine nucleotide exchange factor 4 (EPAC2)
evidence
  • reference
    pubchem.aid:1645886
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1645886, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
0.4467
units
uM
Validation qHTS for antagonist of cAMP-regulated guanine nucleotide exchange factor 4 (EPAC2)OTHER 0.4467 uM; NOT_DETERMINEDUniProtKB:Q8WZA2
pubchem.aid:1645886
assay
Validation qHTS for antagonist of cAMP-regulated guanine nucleotide exchange factor 4 (EPAC2)
evidence
  • reference
    pubchem.aid:1645886
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1645886, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
0.4467
units
uM
target enzyme
UniProtKB:Q8WZA2
Viability Counterscreen for Primary qHTS for Inhibitors of ATXN expressionOTHER 0.1259 uM; NOT_DETERMINEDNot recorded
PMID:35787375
assay
Viability Counterscreen for Primary qHTS for Inhibitors of ATXN expression
evidence
  • reference
    PMID:35787375
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 651635, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
0.1259
units
uM
Viability Counterscreen for Primary qHTS for Inhibitors of ATXN expressionOTHER 0.1259 uM; NOT_DETERMINEDUniProtKB:Q99700
PMID:35787375
assay
Viability Counterscreen for Primary qHTS for Inhibitors of ATXN expression
evidence
  • reference
    PMID:35787375
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 651635, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
0.1259
units
uM
target enzyme
UniProtKB:Q99700
notes
98 further PubChem assay results are in data/raw/pubchem_bioassay.tsv but not written here.

Explore across X-Mech

AntibioticMech (8-azaguanine) ↗ MediaIngredientMech (CHEBI:63486) ↗ TaxonMech: Streptomyces pathocidini (NCBITaxon:1650571) ↗ PathwayMech (mibig:BGC0002508) ↗

Provenance

Source conceptSourceVersion
BGC0002508MIBIG2
CHEBI:63486CHEBI3-star

This page is generated from data/natural_products/alkaloids/8-azaguanine.yaml, which is generated from the committed inventories. Neither is edited by hand.