NaturalProductMech

holomycin

CHEBI:156451 EXACT SEEDED antibacterial

A dithiolopyrrolone antibiotic that is 4,5-dihydro[1,2]dithiolo[4,3-b]pyrrole in which the hydrogens at positions 5 and 6 have been replaced by oxo and acetamido groups, respectively. It is an inhibitor of DNA-dependent RNA polymerase, and exhibits antibacterial and antitumour properties.

Structure

Standard InChIKeyHBUNPJGMNVQSBX-UHFFFAOYSA-N
SMILESCC(=O)NC1=C2C(=CSS2)NC1=O
Stereochemistryfully defined
Cross-referencesnpatlas:NPA013565, pubchem:10262683, chembl:CHEMBL476226

Filing pathway

Alkaloids — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is nrps, from MIBiG.

Producer organisms 3

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

TaxonBasisClusterEvidence
Yersinia ruckeri ATCC 29473
NCBITaxon:527005
BGC_CHARACTERIZED mibig:BGC0002091 PMID:23572522
Photobacterium galatheae
NCBITaxon:1654360
BGC_CHARACTERIZED mibig:BGC0002412 PMID:33771780
Streptomyces clavuligerus ATCC 27064
NCBITaxon:1901
SOURCE_ASSERTION mibig:BGC0000373 PMID:21041678

Occurrences 25

Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.

TaxonSourceReference
Saccharothrix algeriensis
NCBITaxon:173560
CHEBI PMID:32642829
Streptomyces
NCBITaxon:1883
LOTUS DOI:10.7164/ANTIBIOTICS.30.334
Streptomyces albidoflavus
NCBITaxon:1886
LOTUS DOI:10.1007/S10295-013-1348-5
Streptomyces coelicolor
NCBITaxon:1902
LOTUS DOI:10.1007/S10295-013-1348-5
Streptomyces sp.
NCBITaxon:1931
CHEBI PMID:28627048
Photobacterium halotolerans
NCBITaxon:265726
CHEBI PMID:21339958
Yersinia ruckeri
NCBITaxon:29486
LOTUS DOI:10.1074/JBC.M112.448415
Streptomyces flavovirens
NCBITaxon:52258
LOTUS DOI:10.7717/PEERJ.3601
Streptomyces flavogriseus
NCBITaxon:67299
LOTUS DOI:10.7717/PEERJ.3601
Streptomyces flavogriseus
NCBITaxon:67299
CHEBI PMID:28740758
Streptomyces clavuligerus
NCBITaxon:1901
LOTUS DOI:10.1002/CBIC.201200536
Streptomyces clavuligerus
NCBITaxon:1901
LOTUS DOI:10.1007/S00253-013-4721-4
Streptomyces clavuligerus
NCBITaxon:1901
LOTUS DOI:10.1007/S00253-013-5410-Z
Streptomyces clavuligerus
NCBITaxon:1901
LOTUS DOI:10.1016/J.JBIOTEC.2012.09.017
Streptomyces clavuligerus
NCBITaxon:1901
LOTUS DOI:10.1021/BI200321C
Streptomyces clavuligerus
NCBITaxon:1901
LOTUS DOI:10.1073/PNAS.1014140107
Streptomyces clavuligerus
NCBITaxon:1901
LOTUS DOI:10.1111/J.1365-2958.2005.04581.X
Streptomyces clavuligerus
NCBITaxon:1901
LOTUS DOI:10.1128/AEM.00916-15
Streptomyces clavuligerus
NCBITaxon:1901
LOTUS DOI:10.1128/AEM.01701-18
Streptomyces clavuligerus
NCBITaxon:1901
LOTUS DOI:10.1128/AEM.07699-11
Streptomyces clavuligerus
NCBITaxon:1901
LOTUS DOI:10.1128/JB.00859-10
Streptomyces clavuligerus
NCBITaxon:1901
LOTUS DOI:10.1128/JB.184.23.6559-6565.2002
Streptomyces clavuligerus
NCBITaxon:1901
LOTUS DOI:10.1371/JOURNAL.PONE.0033727
Streptomyces clavuligerus
NCBITaxon:1901
CHEBI PMID:21041678
Streptomyces clavuligerus
NCBITaxon:1901
CHEBI PMID:468729

Biosynthetic gene clusters 3

AccessionHostLocus evidenceGenome
mibig:BGC0002091 Yersinia ruckeri ATCC 29473
NCBITaxon:527005
CLUSTER_DEMONSTRATED genbank:ACCC01000028.1
mibig:BGC0002412 Photobacterium galatheae
NCBITaxon:1654360
CLUSTER_DEMONSTRATED genbank:JMIB01000043.1
mibig:BGC0000373 Streptomyces clavuligerus ATCC 27064
NCBITaxon:1901
CLUSTER_UNSTATED genbank:CM000913.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Bioactivities 1

AssayResultReference
USP30 deubiquitinase inhibition: Primary qHTSACTIVEpubchem.aid:1645860

Elsewhere in the fleet

Open questions 1

shared-structure — This structure is reported by more than one MIBiG entry: BGC0000373, BGC0002091, BGC0002412. Confirm they describe the same compound rather than an upstream cross-reference error.

Provenance

Source conceptSourceVersion
BGC0002091MIBIG3
BGC0002412MIBIG2
BGC0000373MIBIG5
CHEBI:156451CHEBI3-star

This page is generated from data/natural_products/alkaloids/holomycin.yaml, which is generated from the committed inventories. Neither is edited by hand.