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staurosporine

naturalproductmech:mibig-c702c4be08 MINTED SEEDED cytotoxicenzyme inhibitor

Grounding review: No 3-star ChEBI entry shares this structure, so the record keeps a content-hashed identifier.
What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

Structure

Standard InChIKeyHKSZLNNOFSGOKW-UHFFFAOYSA-N
SMILESCNC1CC2OC(C)(C1OC)N1C3=CC=CC=C3C3=C4CNC(=O)C4=C4C5=C(C=CC=C5)N2C4=C13
Stereochemistry undefined stereocentres — the InChIKey does not distinguish this compound from its stereoisomers
Cross-referencesnpatlas:NPA014588, pubchem:44259

Filing pathway

Alkaloids — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is other, from MIBiG.

Producer organisms 1

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

TaxonBasisClusterEvidence
Streptomyces sp. TP-A0274
NCBITaxon:171258		 SOURCE_ASSERTION mibig:BGC0000825 PMID:12617516

Occurrences 21

Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.

TaxonSourceReference
Streptomyces
NCBITaxon:1883		 LOTUS DOI:10.7164/ANTIBIOTICS.55.1063
Streptomyces actuosus
NCBITaxon:1885		 LOTUS DOI:10.1080/00021369.1986.10867821
Streptomyces actuosus
NCBITaxon:1885		 LOTUS DOI:10.1248/CPB.51.1402
Streptomyces actuosus
NCBITaxon:1885		 LOTUS DOI:10.7164/ANTIBIOTICS.40.1782
Streptomyces actuosus
NCBITaxon:1885		 LOTUS DOI:10.7164/ANTIBIOTICS.48.143
Streptomyces actuosus
NCBITaxon:1885		 LOTUS DOI:10.7164/ANTIBIOTICS.49.1060
Streptomyces actuosus
NCBITaxon:1885		 LOTUS DOI:10.7164/ANTIBIOTICS.49.519
Streptomyces hygroscopicus
NCBITaxon:1912		 LOTUS DOI:10.1080/00021369.1986.10867821
Streptomyces hygroscopicus
NCBITaxon:1912		 LOTUS DOI:10.1248/CPB.51.1402
Streptomyces hygroscopicus
NCBITaxon:1912		 LOTUS DOI:10.7164/ANTIBIOTICS.40.1782
Streptomyces hygroscopicus
NCBITaxon:1912		 LOTUS DOI:10.7164/ANTIBIOTICS.48.143
Streptomyces hygroscopicus
NCBITaxon:1912		 LOTUS DOI:10.7164/ANTIBIOTICS.49.1060
Streptomyces hygroscopicus
NCBITaxon:1912		 LOTUS DOI:10.7164/ANTIBIOTICS.49.519
Streptomyces longisporoflavus
NCBITaxon:28044		 LOTUS DOI:10.7164/ANTIBIOTICS.48.143
Streptomyces lividus
NCBITaxon:282216		 LOTUS DOI:10.1080/00021369.1986.10867821
Streptomyces lividus
NCBITaxon:282216		 LOTUS DOI:10.1248/CPB.51.1402
Streptomyces lividus
NCBITaxon:282216		 LOTUS DOI:10.7164/ANTIBIOTICS.40.1782
Streptomyces lividus
NCBITaxon:282216		 LOTUS DOI:10.7164/ANTIBIOTICS.48.143
Streptomyces lividus
NCBITaxon:282216		 LOTUS DOI:10.7164/ANTIBIOTICS.49.1060
Streptomyces lividus
NCBITaxon:282216		 LOTUS DOI:10.7164/ANTIBIOTICS.49.519
Lechevalieria aerocolonigenes
NCBITaxon:68170		 LOTUS DOI:10.1271/BBB.67.127

Biosynthetic gene clusters 1

AccessionHostLocus evidenceGenome
mibig:BGC0000825 Streptomyces sp. TP-A0274
NCBITaxon:171258 		CLUSTER_UNSTATED genbank:AB088119.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Mechanism & Biosynthesis Overview

PRODUCERS & OCCURRENCE 1 producer (0 causal) 21 cited occurrences BIOSYNTHETIC LOCUS 1 gene cluster 0 curated pathway steps NATURAL PRODUCT staurosporine naturalproductmech:mibig-c702c4be08 Alkaloids TARGETS & BIOACTIVITY 25 molecular targets 25 bioactivities · 18 graphs

Causal graph — bioactivity 2 nodes, 1 edge

Staurosporine inhibits human PDK1.

SubjectPredicateObjectNotesEvidence
staurosporine inhibits human 3-phosphoinositide-dependent protein kinase 1 BindingDB exact InChIKey: IC50 6.5 nM, residues 51-359; UniProt O15530 = human PDPK1.
DOI:10.1042/BJ20031119
  • reference
    DOI:10.1042/BJ20031119
    evidence type
    PRIMARY_EXPERIMENT
    notes
    PubChem AID 1797581 (BindingDB deposit, PMID 12892559): active; PDK1 kinase activity assay.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — bioactivity 4 nodes, 3 edges

Staurosporine inhibits human AKT1, AKT2, and AKT3.

SubjectPredicateObjectNotesEvidence
staurosporine inhibits human RAC-alpha serine/threonine-protein kinase BindingDB exact InChIKey: engineered human AKT1 139-480 S378A/S381A/T450D/S473D, IC50 1.5 nM; UniProt parent P31749.
DOI:10.1016/j.bmcl.2005.12.065
  • reference
    DOI:10.1016/j.bmcl.2005.12.065
    evidence type
    PRIMARY_EXPERIMENT
    notes
    PubChem AID 1797336 (BindingDB deposit, PMID 16413780): active; AKT1 kinase assay.
staurosporine inhibits human RAC-beta serine/threonine-protein kinase BindingDB exact InChIKey: IC50 6.5 nM; UniProt P31751 = human AKT2.
DOI:10.1016/j.bmcl.2005.12.065
  • reference
    DOI:10.1016/j.bmcl.2005.12.065
    evidence type
    PRIMARY_EXPERIMENT
    notes
    PubChem AID 1797336 (BindingDB deposit, PMID 16413780): active; AKT2 kinase assay.
staurosporine inhibits human RAC-gamma serine/threonine-protein kinase BindingDB exact InChIKey: IC50 10 nM; UniProt Q9Y243 = human AKT3.
DOI:10.1016/j.bmcl.2005.12.065
  • reference
    DOI:10.1016/j.bmcl.2005.12.065
    evidence type
    PRIMARY_EXPERIMENT
    notes
    PubChem AID 1797336 (BindingDB deposit, PMID 16413780): active; AKT3 kinase assay.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — bioactivity 5 nodes, 4 edges

Staurosporine inhibits human PLK1, PLK2, PLK3, and PLK4.

SubjectPredicateObjectNotesEvidence
staurosporine inhibits human serine/threonine-protein kinase PLK1 BindingDB exact InChIKey: Ki 790 nM; UniProt P53350 = human PLK1.
DOI:10.1021/bi7008745
  • reference
    DOI:10.1021/bi7008745
    evidence type
    PRIMARY_EXPERIMENT
    notes
    PubChem AID 1798514 (BindingDB deposit, PMID 17655330): active; PLK1 kinase assay.
staurosporine inhibits human serine/threonine-protein kinase PLK2 BindingDB exact InChIKey: Ki 150 nM; UniProt Q9NYY3 = human PLK2.
DOI:10.1021/bi7008745
  • reference
    DOI:10.1021/bi7008745
    evidence type
    PRIMARY_EXPERIMENT
    notes
    PubChem AID 1798514 (BindingDB deposit, PMID 17655330): active; PLK2 kinase assay.
staurosporine inhibits human serine/threonine-protein kinase PLK3 BindingDB exact InChIKey: Ki 1200 nM; UniProt Q9H4B4 = human PLK3.
DOI:10.1021/bi7008745
  • reference
    DOI:10.1021/bi7008745
    evidence type
    PRIMARY_EXPERIMENT
    notes
    PubChem AID 1798514 (BindingDB deposit, PMID 17655330): active; PLK3 kinase assay.
staurosporine inhibits human serine/threonine-protein kinase PLK4 BindingDB exact InChIKey: Ki 2.6 nM; UniProt O00444 = human PLK4.
DOI:10.1021/bi7008745
  • reference
    DOI:10.1021/bi7008745
    evidence type
    PRIMARY_EXPERIMENT
    notes
    PubChem AID 1798514 (BindingDB deposit, PMID 17655330): active; PLK4 kinase assay.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — bioactivity 2 nodes, 1 edge

Staurosporine inhibits human PIM1.

SubjectPredicateObjectNotesEvidence
staurosporine inhibits human serine/threonine-protein kinase pim-1 BindingDB exact InChIKey: IC50 10 nM, pH 7.6, 30 C; UniProt P11309 = human PIM1.
DOI:10.1074/jbc.M413155200
  • reference
    DOI:10.1074/jbc.M413155200
    evidence type
    PRIMARY_EXPERIMENT
    notes
    PubChem AID 1796959 (BindingDB deposit, PMID 15657054): active; PIM1 kinase assay.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — bioactivity 3 nodes, 2 edges

Staurosporine inhibits human PDGFRB and VEGFR2 receptor tyrosine kinases.

SubjectPredicateObjectNotesEvidence
staurosporine inhibits human platelet-derived growth factor receptor beta BindingDB exact InChIKey: IC50 6.14 nM; UniProt P09619 = human PDGFRB.
DOI:10.1021/cb9002865
  • reference
    DOI:10.1021/cb9002865
    evidence type
    PRIMARY_EXPERIMENT
    notes
    PubChem AID 1799383 (BindingDB deposit, PMID 20020776): active; PDGFRB in-vitro kinase assay.
staurosporine inhibits human vascular endothelial growth factor receptor 2 BindingDB exact InChIKey: IC50 3.29 nM; UniProt P35968 = human KDR.
DOI:10.1021/cb9002865
  • reference
    DOI:10.1021/cb9002865
    evidence type
    PRIMARY_EXPERIMENT
    notes
    PubChem AID 1799383 (BindingDB deposit, PMID 20020776): active; VEGFR2 in-vitro kinase assay.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — bioactivity 2 nodes, 1 edge

Staurosporine inhibits chicken c-Src.

SubjectPredicateObjectNotesEvidence
staurosporine inhibits chicken proto-oncogene tyrosine-protein kinase Src BindingDB exact InChIKey: KD 8 nM; UniProt P00523 = Gallus gallus SRC.
DOI:10.1038/nchembio.162
  • reference
    DOI:10.1038/nchembio.162
    evidence type
    PRIMARY_EXPERIMENT
    notes
    PubChem AID 1799403 (BindingDB deposit, PMID 19396179): active; wild-type cSrc tyrosine kinase inhibition assay.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — bioactivity 2 nodes, 1 edge

Staurosporine inhibits Plasmodium falciparum K1 calcium-dependent protein kinase 1.

SubjectPredicateObjectNotesEvidence
staurosporine inhibits Plasmodium falciparum K1 calcium-dependent protein kinase 1 BindingDB exact InChIKey: IC50 240 nM, pH 7.5, 24 C; UniProt P62343 = P. falciparum K1 CDPK1.
DOI:10.1038/nchembio.87
  • reference
    DOI:10.1038/nchembio.87
    evidence type
    PRIMARY_EXPERIMENT
    notes
    PubChem AID 1799368 (BindingDB deposit, PMID 18454143): active; P. falciparum CDPK1 scintillation-proximity assay.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — bioactivity 2 nodes, 1 edge

Staurosporine inhibits human GSK3B.

SubjectPredicateObjectNotesEvidence
staurosporine inhibits human glycogen synthase kinase-3 beta BindingDB exact InChIKey: IC50 38, 60 and 54 nM; UniProt P49841 = human GSK3B.
DOI:10.1111/cbdd.12546
DOI:10.1111/cbdd.12724
DOI:10.1016/j.bioorg.2016.07.007
  • reference
    DOI:10.1111/cbdd.12546
    evidence type
    PRIMARY_EXPERIMENT
    notes
    PubChem AID 1801382 (BindingDB deposit, PMID 25711384): active; GSK-3 beta kinase assay.
  • reference
    DOI:10.1111/cbdd.12724
    evidence type
    PRIMARY_EXPERIMENT
    notes
    PubChem AID 1801812 (BindingDB deposit, PMID 26804375): active; Kinase-Glo assay.
  • reference
    DOI:10.1016/j.bioorg.2016.07.007
    evidence type
    PRIMARY_EXPERIMENT
    notes
    PubChem AID 1801940 (BindingDB deposit, PMID 27454617): active; GSK-3 beta activity assay.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — bioactivity 2 nodes, 1 edge

Staurosporine inhibits human CSF1R.

SubjectPredicateObjectNotesEvidence
staurosporine inhibits human macrophage colony-stimulating factor 1 receptor BindingDB exact InChIKey: IC50 49, 57 nM, one construct covering residues 538-678 and 753-922, pH 7.5, 22 C; UniProt parent P07333 = human CSF1R.
DOI:10.1074/jbc.M608182200
  • reference
    DOI:10.1074/jbc.M608182200
    evidence type
    PRIMARY_EXPERIMENT
    notes
    PubChem AID 1797654 (BindingDB deposit, PMID 17132625): active; cFMS autophosphorylation fluorescence-polarization endpoint assay.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — bioactivity 2 nodes, 1 edge

Staurosporine inhibits bovine cAMP-dependent protein kinase catalytic subunit alpha.

SubjectPredicateObjectNotesEvidence
staurosporine inhibits bovine cAMP-dependent protein kinase catalytic subunit alpha BindingDB exact stereo-incomplete InChIKey: IC50 40, 3.6 nM; UniProt P00517 = Bos taurus PRKACA.
DOI:10.1016/0960-894X(94)00458-R
DOI:10.1016/j.bmcl.2005.12.065
  • reference
    DOI:10.1016/0960-894X(94)00458-R
    evidence type
    PRIMARY_EXPERIMENT
    notes
    BindingDB: this staurosporine structure, bovine PKA C-alpha, IC50 40 nM.
  • reference
    DOI:10.1016/j.bmcl.2005.12.065
    evidence type
    PRIMARY_EXPERIMENT
    notes
    PubChem AID 1797337 (BindingDB deposit, PMID 16413780): active; PKA C-alpha PKA/PKC kinase assay.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — bioactivity 2 nodes, 1 edge

Staurosporine inhibits human ITK/TSK.

SubjectPredicateObjectNotesEvidence
staurosporine inhibits human tyrosine-protein kinase ITK/TSK BindingDB rows 60-61, exact stereo-incomplete InChIKey: active, Ki 2.2 and <10 nM, pH 8, 25 C; UniProt Q08881 = human ITK.
DOI:10.1074/jbc.M400031200
  • reference
    DOI:10.1074/jbc.M400031200
    evidence type
    PRIMARY_EXPERIMENT
    notes
    BindingDB-deposited PubChem AID 1798742, PMID 14766749: staurosporine, human ITK/TSK radiometric kinase Ki assay.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — bioactivity 3 nodes, 2 edges

Staurosporine inhibits human PKC gamma and PKC zeta.

SubjectPredicateObjectNotesEvidence
staurosporine inhibits human protein kinase C gamma type BindingDB exact stereo-incomplete InChIKey: IC50 6.6 nM, pH 7.4, 22 C; UniProt P05129 = human PRKCG.
DOI:10.1016/j.bmcl.2005.12.065
  • reference
    DOI:10.1016/j.bmcl.2005.12.065
    evidence type
    PRIMARY_EXPERIMENT
    notes
    BindingDB row 44; its PubChem AID 1797337, row 2326; PMID 16413780: active; PKC gamma PKA/PKC kinase assay.
staurosporine inhibits human protein kinase C zeta type BindingDB exact stereo-incomplete InChIKey: IC50 570 nM, pH 7.4, 22 C; UniProt Q05513 = human PRKCZ.
DOI:10.1016/j.bmcl.2005.12.065
  • reference
    DOI:10.1016/j.bmcl.2005.12.065
    evidence type
    PRIMARY_EXPERIMENT
    notes
    BindingDB row 45; its PubChem AID 1797337, row 2327; PMID 16413780: active; PKC zeta PKA/PKC kinase assay.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — bioactivity 2 nodes, 1 edge

Staurosporine inhibits bovine ROCK2.

SubjectPredicateObjectNotesEvidence
staurosporine inhibits bovine Rho-associated protein kinase 2 BindingDB exact stereo-incomplete InChIKey: IC50 1.2 nM; UniProt Q28021 = Bos taurus ROCK2.
DOI:10.1074/jbc.M512374200
  • reference
    DOI:10.1074/jbc.M512374200
    evidence type
    PRIMARY_EXPERIMENT
    notes
    BindingDB row 50; its PubChem AID 1797135, row 2321; PMID 16699172: active; ROCK2 kinase inhibition assay.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — bioactivity 3 nodes, 2 edges

Staurosporine inhibits human Chk1 and Chk2.

SubjectPredicateObjectNotesEvidence
staurosporine inhibits human serine/threonine-protein kinase Chk1 BindingDB exact stereo-incomplete InChIKey: Ki 7.8 nM, pH 7.4, 37 C; UniProt O14757 = human CHEK1.
DOI:10.1074/jbc.M201233200
  • reference
    DOI:10.1074/jbc.M201233200
    evidence type
    PRIMARY_EXPERIMENT
    notes
    BindingDB row 51; its PubChem AID 1797591, row 2330; PMID 12244092: active; Chk1 enzymatic assay.
staurosporine inhibits human serine/threonine-protein kinase Chk2 BindingDB row 52/PubChem AID 1799260 report 80 nM IC50 for staurosporine versus human Chk2 [209-531,Q209M] at pH 7.5, 30 C; O96017 is human CHEK2.
DOI:10.1124/mol.107.035832
  • reference
    DOI:10.1124/mol.107.035832
    evidence type
    PRIMARY_EXPERIMENT
    notes
    BindingDB row 52 and PubChem row 2345 cite PMID 17616632.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — bioactivity 2 nodes, 1 edge

Staurosporine inhibits chicken smooth muscle myosin light chain kinase.

SubjectPredicateObjectNotesEvidence
staurosporine inhibits chicken smooth muscle myosin light chain kinase BindingDB exact stereo-incomplete InChIKey: IC50 10 nM, pH 7.2, 30 C; UniProt P11799 = Gallus gallus Mylk.
DOI:10.1016/0960-894X(94)00458-R
  • reference
    DOI:10.1016/0960-894X(94)00458-R
    evidence type
    PRIMARY_EXPERIMENT
    notes
    BindingDB row 37; its PubChem AID 1795448, row 2308: active; chicken smooth-muscle MLCK assay.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — bioactivity 2 nodes, 1 edge

Staurosporine inhibits bovine cGMP-dependent protein kinase 1.

SubjectPredicateObjectNotesEvidence
staurosporine inhibits bovine cGMP-dependent protein kinase 1 BindingDB exact stereo-incomplete InChIKey: IC50 18 nM; UniProt P00516 = Bos taurus PRKG1.
DOI:10.1016/0960-894X(94)00458-R
  • reference
    DOI:10.1016/0960-894X(94)00458-R
    evidence type
    PRIMARY_EXPERIMENT
    notes
    BindingDB row 78; its PubChem AID 1795450, row 2311: active; cGMP-dependent protein kinase 1 cGPK assay.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — bioactivity 2 nodes, 1 edge

Staurosporine inhibits rat PKC beta.

SubjectPredicateObjectNotesEvidence
staurosporine inhibits rat protein kinase C beta type BindingDB exact stereo-incomplete InChIKey: IC50 9 nM, Ki 19 nM; UniProt P68403 = Rattus norvegicus Prkcb.
DOI:10.1016/0960-894X(94)00458-R
DOI:10.1021/jm00079a024
DOI:10.1021/jm00053a003
DOI:10.1016/1074-5521(95)90124-8
  • reference
    DOI:10.1016/0960-894X(94)00458-R
    evidence type
    PRIMARY_EXPERIMENT
    notes
    BindingDB row 40: staurosporine, rat PKC beta, IC50 9 nM.
  • reference
    DOI:10.1021/jm00079a024
    evidence type
    PRIMARY_EXPERIMENT
    notes
    BindingDB row 41, PubChem row 2305, PMID 1732526: active; rat PKC beta PKC assay.
  • reference
    DOI:10.1021/jm00053a003
    evidence type
    PRIMARY_EXPERIMENT
    notes
    BindingDB row 42, PubChem row 2306, PMID 8421286: active; rat PKC beta PKC assay.
  • reference
    DOI:10.1016/1074-5521(95)90124-8
    evidence type
    PRIMARY_EXPERIMENT
    notes
    BindingDB row 43, PubChem row 2355, PMID 9383464: active; rat PKC beta inhibition assay.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 8 nodes, 8 edges

Partial TP-A0274 aglycone evidence: C-His StaP crystallography, separately purified C-His StaP/StaC tandem assays, and staJ-deletion cultures. IDs denote canonical parents, not constructs. No stable complex, direct StaC substrate, radical mechanism or sugar stereochemistry inferred.

SubjectPredicateObjectNotesEvidence
TP-A0274 StaP canonical protein binds at its active site Chromopyrrolic acid 2Z3U (2.4 A): Q83WG3 (417 aa) plus LEHHHHHH, 425 aa total. Only active-site CPA interpreted; two other sites may be packing artifacts.
DOI:10.1073/pnas.0702946104
https://www.rcsb.org/structure/2Z3U
  • reference
    DOI:10.1073/pnas.0702946104
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    the functional significance of the latter two molecules is unclear
  • reference
    https://www.rcsb.org/structure/2Z3U
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-03
TP-A0274 StaP canonical protein participates in StaP/StaC tandem reaction 417-aa canonical parent = AB088119.1/BAC55212.1; preparation as in the 2007 StaP study.
DOI:10.1271/bbb.110474
  • reference
    DOI:10.1271/bbb.110474
    evidence type
    PRIMARY_EXPERIMENT
TP-A0274 StaC canonical protein contributes to K252c formation in StaP/StaC tandem reaction 545-aa canonical parent = AB088119.1/BAF47693.1; both cloning-primer targets match. C-His tag is not part of UniProt.
DOI:10.1271/bbb.110474
  • reference
    DOI:10.1271/bbb.110474
    evidence type
    PRIMARY_EXPERIMENT
Chromopyrrolic acid is supplied to StaP/StaC tandem reaction Fig. 4A: 1 uM StaP, 5 uM StaC, 65 uM CPA; 75 mM HEPES pH 8, 30 C, 5 min; spinach ferredoxin/reductase and NADH.
DOI:10.1271/bbb.110474
  • reference
    DOI:10.1271/bbb.110474
    evidence type
    PRIMARY_EXPERIMENT
Added FAD enhances K252c output in StaP/StaC tandem reaction Fig. 4A: output rises up to 0.5 uM added FAD, then falls. Tandem response, not a direct FAD-binding measurement.
DOI:10.1271/bbb.110474
  • reference
    DOI:10.1271/bbb.110474
    evidence type
    PRIMARY_EXPERIMENT
    snippet
    Exogenous FAD increased K252c production
StaP/StaC tandem reaction produces predominantly K252c (staurosporine aglycone) Fig. 4A; HPLC against NMR/LC-MS-verified standards. No stable StaC substrate or elementary mechanism assigned.
DOI:10.1271/bbb.110474
  • reference
    DOI:10.1271/bbb.110474
    evidence type
    PRIMARY_EXPERIMENT
TP-A0274 staJ in-frame deletion strain has in-frame deletion in the gene encoding TP-A0274 StaJ wild-type protein 472-aa parent = AB088119.1/BAC55214.1, not the mutant allele. All four deletion-primer targets match.
DOI:10.1271/bbb.110474
  • reference
    DOI:10.1271/bbb.110474
    evidence type
    PRIMARY_EXPERIMENT
TP-A0274 staJ in-frame deletion strain yielded isolated K252c (staurosporine aglycone) Methods: 5 days, 30 C; 128 mg purified from 4 L by extraction/HPLC, not a fermentation titer. NMR/LC-MS identity, [M+H]+ 312.
DOI:10.1271/bbb.110474
  • reference
    DOI:10.1271/bbb.110474
    evidence type
    PRIMARY_EXPERIMENT

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Molecular targets 25

TargetTarget organismMeasurementEvidence
3-phosphoinositide-dependent protein kinase 1 [51-359]
PROTEIN BINDS
UniProtKB:O15530		 Homo sapiens
NCBITaxon:9606		 IC50 6.5 nM
PMID:12892559
  • reference
    PMID:12892559
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; measured at pH 7.5000, 30.00 C C; qualifier EQUAL.
5'-AMP-activated protein kinase subunit beta-1/5'-AMP-activated protein kinase subunit gamma-1/Dual specificity protein kinase CLK2
PROTEIN BINDS
UniProtKB:P49760		 Homo sapiens
NCBITaxon:9606		 IC50 1 nM
PMID:20020776
  • reference
    PMID:20020776
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; qualifier LESS_THAN.
Activin receptor type-1
PROTEIN BINDS
UniProtKB:Q04771		 Homo sapiens
NCBITaxon:9606		 IC50 4531 nM
PMID:20020776
  • reference
    PMID:20020776
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; qualifier EQUAL.
Calcium-dependent protein kinase 1
PROTEIN BINDS
UniProtKB:P62343		 Plasmodium falciparum (isolate K1 / Thailand)
NCBITaxon:5839		 IC50 240 nM
PMID:18454143
  • reference
    PMID:18454143
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; measured at pH 7.5000, 24.00 C C; qualifier EQUAL.
Cyclin-A2/Cyclin-dependent kinase 1
PROTEIN BINDS
UniProtKB:P06493		 Homo sapiens
NCBITaxon:9606		 KI 1 nM
PMID:12244092
  • reference
    PMID:12244092
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; measured at pH 7.4000, 22.00 C C; qualifier EQUAL.
Cyclin-A2/Cyclin-dependent kinase 2
PROTEIN BINDS
UniProtKB:P24941		 Homo sapiens
NCBITaxon:9606		 KI 2.9 nM
PMID:12244092
  • reference
    PMID:12244092
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; measured at pH 7.4000, 22.00 C C; qualifier EQUAL.
Cyclin-dependent kinase 4/G1/S-specific cyclin-D1
PROTEIN BINDS
UniProtKB:P11802		 Homo sapiens
NCBITaxon:9606		 KI 41 nM
PMID:12244092
  • reference
    PMID:12244092
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; measured at pH 7.5000, 30.00 C C; qualifier EQUAL.
Cyclin-dependent kinase 4/G1/S-specific cyclin-D1
PROTEIN BINDS
UniProtKB:P11802		 Homo sapiens
NCBITaxon:9606		 IC50 59 nM
PMID:12824014
  • reference
    PMID:12824014
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; measured at pH 7.0000, 22.00 C C; qualifier EQUAL.
Glycogen synthase kinase-3 beta
PROTEIN BINDS
UniProtKB:P49841		 Homo sapiens
NCBITaxon:9606		 IC50 38 nM
PMID:25711384
  • reference
    PMID:25711384
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; qualifier EQUAL.
Glycogen synthase kinase-3 beta
PROTEIN BINDS
UniProtKB:P49841		 Homo sapiens
NCBITaxon:9606		 IC50 60 nM
PMID:26804375
  • reference
    PMID:26804375
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; measured at 30.00 C C; qualifier EQUAL.
Glycogen synthase kinase-3 beta
PROTEIN BINDS
UniProtKB:P49841		 Homo sapiens
NCBITaxon:9606		 IC50 54 nM
PMID:27454617
  • reference
    PMID:27454617
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; qualifier EQUAL.
Macrophage colony-stimulating factor 1 receptor [538-678,753-922]
PROTEIN BINDS
UniProtKB:P07333		 Homo sapiens
NCBITaxon:9606		 IC50 49 nM
PMID:17132625
  • reference
    PMID:17132625
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; measured at pH 7.5000, 22.00 C C; qualifier EQUAL.
Macrophage colony-stimulating factor 1 receptor [538-678,753-922]
PROTEIN BINDS
UniProtKB:P07333		 Homo sapiens
NCBITaxon:9606		 IC50 57 nM
PMID:17132625
  • reference
    PMID:17132625
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; measured at pH 7.5000, 22.00 C C; qualifier EQUAL.
Macrophage colony-stimulating factor 1 receptor [538-972]
PROTEIN BINDS
UniProtKB:P07333		 IC50 57 nM
PMID:17132625
  • reference
    PMID:17132625
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; measured at pH 7.5000, 22.00 C C; qualifier EQUAL.
Myosin light chain kinase, smooth muscle
PROTEIN BINDS
UniProtKB:P11799		 Gallus gallus
NCBITaxon:9031		 IC50 10 nM
DOI:10.1016/0960-894X(94)00458-R
  • reference
    DOI:10.1016/0960-894X(94)00458-R
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; measured at pH 7.2000, 30.00 C C; qualifier EQUAL.
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
PROTEIN BINDS
UniProtKB:P48736		 Homo sapiens
NCBITaxon:9606		 KD 290 nM
PMID:11090628
  • reference
    PMID:11090628
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; measured at pH 7.2000, 20.00 C C; qualifier EQUAL.
Platelet-derived growth factor receptor beta
PROTEIN BINDS
UniProtKB:P09619		 Homo sapiens
NCBITaxon:9606		 IC50 6.14 nM
PMID:20020776
  • reference
    PMID:20020776
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; qualifier EQUAL.
Protein kinase C beta type
PROTEIN BINDS
UniProtKB:P68403		 Rattus norvegicus
NCBITaxon:10116		 IC50 9 nM
DOI:10.1016/0960-894X(94)00458-R
  • reference
    DOI:10.1016/0960-894X(94)00458-R
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; qualifier EQUAL.
Protein kinase C beta type
PROTEIN BINDS
UniProtKB:P68403		 Rattus norvegicus
NCBITaxon:10116		 IC50 9 nM
PMID:1732526
  • reference
    PMID:1732526
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; measured at pH 7.5000, 30.00 C C; qualifier EQUAL.
Protein kinase C beta type
PROTEIN BINDS
UniProtKB:P68403		 Rattus norvegicus
NCBITaxon:10116		 IC50 9 nM
PMID:8421286
  • reference
    PMID:8421286
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; measured at pH 7.5000, 30.00 C C; qualifier EQUAL.
Protein kinase C beta type
PROTEIN BINDS
UniProtKB:P68403		 Rattus norvegicus
NCBITaxon:10116		 KI 19 nM
PMID:9383464
  • reference
    PMID:9383464
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; measured at 30.00 C C; qualifier EQUAL.
Protein kinase C gamma type
PROTEIN BINDS
UniProtKB:P05129		 Homo sapiens
NCBITaxon:9606		 IC50 6.6 nM
PMID:16413780
  • reference
    PMID:16413780
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; measured at pH 7.4000, 22.00 C C; qualifier EQUAL.
Protein kinase C zeta type
PROTEIN BINDS
UniProtKB:Q05513		 Homo sapiens
NCBITaxon:9606		 IC50 570 nM
PMID:16413780
  • reference
    PMID:16413780
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; measured at pH 7.4000, 22.00 C C; qualifier EQUAL.
Proto-oncogene tyrosine-protein kinase Src
PROTEIN BINDS
UniProtKB:P00523		 Gallus gallus
NCBITaxon:9031		 KD 8 nM
PMID:19396179
  • reference
    PMID:19396179
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; qualifier EQUAL.
RAC-alpha serine/threonine-protein kinase [139-480,S378A,S381A,T450D,S473D]
PROTEIN BINDS
UniProtKB:P31749

31 further BindingDB target rows are in data/raw/bindingdb_targets.tsv but not written here.

 Homo sapiens
NCBITaxon:9606		 IC50 1.5 nM
PMID:16413780
  • reference
    PMID:16413780
    evidence type
    DATABASE_ASSERTION
    notes
    BindingDB own-curated affinity; measured at pH 7.5000, 22.00 C C; qualifier EQUAL.

Bioactivities 25

Entries preserve source qualifiers, targets and evidence. Repeated assay names or values are not independent replications; inspect source detail for differences.

AssayResultTarget enzymeSource detail
A549 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-06_Inhibitor_Dose_DryPowder_Activity_Set6OTHER 26.77 uM; ACTIVENot recorded
pubchem.aid:743312
assay
A549 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-06_Inhibitor_Dose_DryPowder_Activity_Set6
evidence
  • reference
    pubchem.aid:743312
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 743312, deposited by Broad Institute; assay type Confirmatory. Source endpoint: AC50.
call
ACTIVE
measurement type
OTHER
value
26.77
units
uM
activity class
CYTOTOXIC
ABL1 kinase (ABL1-N-HiBiT) SDR gain of signal screenOTHER 0.8802 uM; ACTIVENot recorded
pubchem.aid:1963315
assay
ABL1 kinase (ABL1-N-HiBiT) SDR gain of signal screen
evidence
  • reference
    pubchem.aid:1963315
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963315, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
0.8802
units
uM
ABL1 kinase (ABL1-N-HiBiT) SDR gain of signal screenOTHER 0.8802 uM; ACTIVEUniProtKB:P00519
pubchem.aid:1963315
assay
ABL1 kinase (ABL1-N-HiBiT) SDR gain of signal screen
evidence
  • reference
    pubchem.aid:1963315
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963315, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
0.8802
units
uM
target enzyme
UniProtKB:P00519
ABL1 kinase (ABL1-N-HiBiT) SDR gain of signal screen - plus ATPOTHER 0.6112 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1963316
assay
ABL1 kinase (ABL1-N-HiBiT) SDR gain of signal screen - plus ATP
evidence
  • reference
    pubchem.aid:1963316
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963316, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
0.6112
units
uM
ABL1 kinase (ABL1-N-HiBiT) SDR gain of signal screen - plus ATPOTHER 0.6112 uM; NOT_DETERMINEDUniProtKB:P00519
pubchem.aid:1963316
assay
ABL1 kinase (ABL1-N-HiBiT) SDR gain of signal screen - plus ATP
evidence
  • reference
    pubchem.aid:1963316
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963316, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
0.6112
units
uM
target enzyme
UniProtKB:P00519
ABL1 kinase (ABL1-N-HiBiT) enzymatic inhibition screen - Functional assayOTHER 3.0633 uM; ACTIVENot recorded
pubchem.aid:1963317
assay
ABL1 kinase (ABL1-N-HiBiT) enzymatic inhibition screen - Functional assay
evidence
  • reference
    pubchem.aid:1963317
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963317, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
3.0633
units
uM
ABL1 kinase (ABL1-N-HiBiT) enzymatic inhibition screen - Functional assayOTHER 3.0633 uM; ACTIVEUniProtKB:P00519
pubchem.aid:1963317
assay
ABL1 kinase (ABL1-N-HiBiT) enzymatic inhibition screen - Functional assay
evidence
  • reference
    pubchem.aid:1963317
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963317, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
3.0633
units
uM
target enzyme
UniProtKB:P00519
Akt Kinase Assay from Article 10.1016/j.bmcl.2005.12.065: "Synthesis and structure-activity relationship of 3,4'-bispyridinylethylenes: discovery of a potent 3-isoquinolinylpyridine inhibitor of protein kinase B (PKB/Akt) for the treatment of cancer."IC50 0.0015 uM; ACTIVEUniProtKB:P31749
PMID:16413780
assay
Akt Kinase Assay from Article 10.1016/j.bmcl.2005.12.065: "Synthesis and structure-activity relationship of 3,4'-bispyridinylethylenes: discovery of a potent 3-isoquinolinylpyridine inhibitor of protein kinase B (PKB/Akt) for the treatment of cancer."
evidence
  • reference
    PMID:16413780
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1797336, deposited by BindingDB; assay type Confirmatory.
call
ACTIVE
measurement type
IC50
value
0.0015
units
uM
target enzyme
UniProtKB:P31749
activity class
CYTOTOXIC
Akt Kinase Assay from Article 10.1016/j.bmcl.2005.12.065: "Synthesis and structure-activity relationship of 3,4'-bispyridinylethylenes: discovery of a potent 3-isoquinolinylpyridine inhibitor of protein kinase B (PKB/Akt) for the treatment of cancer."IC50 0.0065 uM; ACTIVEUniProtKB:P31751
PMID:16413780
assay
Akt Kinase Assay from Article 10.1016/j.bmcl.2005.12.065: "Synthesis and structure-activity relationship of 3,4'-bispyridinylethylenes: discovery of a potent 3-isoquinolinylpyridine inhibitor of protein kinase B (PKB/Akt) for the treatment of cancer."
evidence
  • reference
    PMID:16413780
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1797336, deposited by BindingDB; assay type Confirmatory.
call
ACTIVE
measurement type
IC50
value
0.0065
units
uM
target enzyme
UniProtKB:P31751
activity class
CYTOTOXIC
Akt Kinase Assay from Article 10.1016/j.bmcl.2005.12.065: "Synthesis and structure-activity relationship of 3,4'-bispyridinylethylenes: discovery of a potent 3-isoquinolinylpyridine inhibitor of protein kinase B (PKB/Akt) for the treatment of cancer."IC50 0.01 uM; ACTIVEUniProtKB:Q9Y243
PMID:16413780
assay
Akt Kinase Assay from Article 10.1016/j.bmcl.2005.12.065: "Synthesis and structure-activity relationship of 3,4'-bispyridinylethylenes: discovery of a potent 3-isoquinolinylpyridine inhibitor of protein kinase B (PKB/Akt) for the treatment of cancer."
evidence
  • reference
    PMID:16413780
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1797336, deposited by BindingDB; assay type Confirmatory.
call
ACTIVE
measurement type
IC50
value
0.01
units
uM
target enzyme
UniProtKB:Q9Y243
activity class
CYTOTOXIC
Assay for Inhibitors of the ERK Signaling Pathway using a Homogeneous Screening Assay: EGFR Kinase InhibitionOTHER 0.3981 uM; ACTIVENot recorded
pubchem.aid:1731
assay
Assay for Inhibitors of the ERK Signaling Pathway using a Homogeneous Screening Assay: EGFR Kinase Inhibition
evidence
  • reference
    pubchem.aid:1731
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1731, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. Target accession NP_005219 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
ACTIVE
measurement type
OTHER
value
0.3981
units
uM
activity class
ENZYME_INHIBITOR
Assay for Inhibitors of the ERK Signaling Pathway using a Homogeneous Screening Assay: EGFR L858R Kinase InhibitionOTHER 0.3548 uM; ACTIVENot recorded
pubchem.aid:1727
assay
Assay for Inhibitors of the ERK Signaling Pathway using a Homogeneous Screening Assay: EGFR L858R Kinase Inhibition
evidence
  • reference
    pubchem.aid:1727
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1727, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. Target accession NP_005219 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
ACTIVE
measurement type
OTHER
value
0.3548
units
uM
activity class
ENZYME_INHIBITOR
Assay for Inhibitors of the ERK Signaling Pathway using a Homogeneous Screening Assay: EGFR T790M Kinase InhibitionOTHER 0.0056 uM; ACTIVENot recorded
pubchem.aid:1729
assay
Assay for Inhibitors of the ERK Signaling Pathway using a Homogeneous Screening Assay: EGFR T790M Kinase Inhibition
evidence
  • reference
    pubchem.aid:1729
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1729, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. Target accession NP_005219 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
ACTIVE
measurement type
OTHER
value
0.0056
units
uM
activity class
ENZYME_INHIBITOR
Assay for Inhibitors of the ERK Signaling Pathway using a Homogeneous Screening Assay: EGFR T790M/L858R Kinase InhibitionOTHER 0.0398 uM; ACTIVENot recorded
pubchem.aid:1726
assay
Assay for Inhibitors of the ERK Signaling Pathway using a Homogeneous Screening Assay: EGFR T790M/L858R Kinase Inhibition
evidence
  • reference
    pubchem.aid:1726
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1726, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. Target accession NP_005219 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
ACTIVE
measurement type
OTHER
value
0.0398
units
uM
activity class
ENZYME_INHIBITOR
Assay for Inhibitors of the ERK Signaling Pathway using a Homogeneous Screening Assay: MEK InhibitionOTHER 0.1 uM; ACTIVENot recorded
pubchem.aid:1732
assay
Assay for Inhibitors of the ERK Signaling Pathway using a Homogeneous Screening Assay: MEK Inhibition
evidence
  • reference
    pubchem.aid:1732
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1732, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. Target accession NP_002746 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
ACTIVE
measurement type
OTHER
value
0.1
units
uM
activity class
ENZYME_INHIBITOR
Assay for Inhibitors of the ERK Signaling Pathway using a Homogeneous Screening Assay: c-Raf InhibitionOTHER 0.1778 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1728
assay
Assay for Inhibitors of the ERK Signaling Pathway using a Homogeneous Screening Assay: c-Raf Inhibition
evidence
  • reference
    pubchem.aid:1728
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1728, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. Target accession NP_002871 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
NOT_DETERMINED
measurement type
OTHER
value
0.1778
units
uM
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: AML-191 cell lineOTHER 0.0295 uM; ACTIVENot recorded
pubchem.aid:2202236
assay
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: AML-191 cell line
evidence
  • reference
    pubchem.aid:2202236
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2202236, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
0.0295
units
uM
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: AML-194 cell lineOTHER 0.0332 uM; ACTIVENot recorded
pubchem.aid:2202237
assay
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: AML-194 cell line
evidence
  • reference
    pubchem.aid:2202237
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2202237, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
0.0332
units
uM
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: HNT-34 cell lineOTHER 0.0934 uM; ACTIVENot recorded
pubchem.aid:2202234
assay
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: HNT-34 cell line
evidence
  • reference
    pubchem.aid:2202234
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2202234, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
0.0934
units
uM
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: MV-4-11 cell lineOTHER 0.0074 uM; ACTIVENot recorded
pubchem.aid:2202233
assay
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: MV-4-11 cell line
evidence
  • reference
    pubchem.aid:2202233
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2202233, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
0.0074
units
uM
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: OCI-AML3 cell lineOTHER 0.0833 uM; ACTIVENot recorded
pubchem.aid:2202232
assay
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: OCI-AML3 cell line
evidence
  • reference
    pubchem.aid:2202232
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2202232, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
0.0833
units
uM
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: SET-2 cell lineOTHER 0.1864 uM; ACTIVENot recorded
pubchem.aid:2202231
assay
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: SET-2 cell line
evidence
  • reference
    pubchem.aid:2202231
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2202231, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
0.1864
units
uM
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: UCSD-AML1 cell lineOTHER 0.0661 uM; ACTIVENot recorded
pubchem.aid:2202235
assay
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: UCSD-AML1 cell line
evidence
  • reference
    pubchem.aid:2202235
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2202235, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
0.0661
units
uM
Biochemical Assay from US Patent US20240002365: "PYRIDAZINE AND 1,2,4-TRIAZINE DERIVATIVES AS FGFR KINASE INHIBITORS"IC50 0.0021 uM; ACTIVEUniProtKB:P11362
pubchem.aid:1920143
assay
Biochemical Assay from US Patent US20240002365: "PYRIDAZINE AND 1,2,4-TRIAZINE DERIVATIVES AS FGFR KINASE INHIBITORS"
evidence
  • reference
    pubchem.aid:1920143
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1920143, deposited by BindingDB; assay type Confirmatory.
call
ACTIVE
measurement type
IC50
value
0.0021
units
uM
target enzyme
UniProtKB:P11362
Biochemical Assay from US Patent US20240002365: "PYRIDAZINE AND 1,2,4-TRIAZINE DERIVATIVES AS FGFR KINASE INHIBITORS"IC50 0.0008 uM; ACTIVEUniProtKB:P21802
pubchem.aid:1920143
assay
Biochemical Assay from US Patent US20240002365: "PYRIDAZINE AND 1,2,4-TRIAZINE DERIVATIVES AS FGFR KINASE INHIBITORS"
evidence
  • reference
    pubchem.aid:1920143
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1920143, deposited by BindingDB; assay type Confirmatory.
call
ACTIVE
measurement type
IC50
value
0.0008
units
uM
target enzyme
UniProtKB:P21802
notes
523 further PubChem assay results are in data/raw/pubchem_bioassay.tsv but not written here.

Explore across X-Mech

AntibioticMech (staurosporine) ↗ MediaIngredientMech (naturalproductmech:mibig-c702c4be08) ↗ TaxonMech: Streptomyces sp. TP-A0274 (NCBITaxon:171258) ↗ PathwayMech (mibig:BGC0000825) ↗

Provenance

Source conceptSourceVersion
BGC0000825MIBIG5

This page is generated from data/natural_products/alkaloids/staurosporine-2.yaml, which is generated from the committed inventories. Neither is edited by hand.