NaturalProductMech

toxoflavin

CHEBI:80729 EXACT SEEDED antibacterialcytotoxicenzyme inhibitor

A pyrimidotriazine that is 1,6-dimethyl-1,5,6,7-tetrahydropyrimido[5,4-e][1,2,4]triazine with oxo groups at positions 5 and 7.

Structure

Standard InChIKeySLGRAIAQIAUZAQ-UHFFFAOYSA-N
SMILESCN1N=CN=C2C(=O)N(C)C(=O)N=C12
Stereochemistryfully defined
Cross-referencesnpatlas:NPA04485, pubchem:66541

Filing pathway

Alkaloids — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is other, from MIBiG.

Producer organisms 2

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

TaxonBasisClusterEvidence
Burkholderia glumae
NCBITaxon:337
SOURCE_ASSERTION mibig:BGC0000929 DOI:10.1007/s10327-003-0096-1
Streptomyces hiroshimensis
NCBITaxon:66424
SOURCE_ASSERTION mibig:BGC0001972 PMID:30565634

Occurrences 4

Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.

TaxonSourceReference
Burkholderia gladioli
NCBITaxon:28095
CHEBI PMID:5905124
Burkholderia glumae
NCBITaxon:337
LOTUS DOI:10.1007/S10327-003-0096-1
Burkholderia glumae
NCBITaxon:337
CHEBI Jeong, Y., Kim, J., Kim, S., and Kang, Y (2003). Toxoflavin Produced by Burkholderia glumae Causing Rice Grain Rot Is Responsible for Inducing Bacterial Wilt in Many Field Crops. Plant Disease, Vol. 87 (8). p 890-895.
Streptomyces hiroshimensis
NCBITaxon:66424
LOTUS DOI:10.1039/C8OB02847H

Biosynthetic gene clusters 2

AccessionHostLocus evidenceGenome
mibig:BGC0000929 Burkholderia glumae
NCBITaxon:337
CLUSTER_UNSTATED genbank:EF587764.1
mibig:BGC0001972 Streptomyces hiroshimensis
NCBITaxon:66424
CLUSTER_UNSTATED genbank:MK256951.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Bioactivities 25

AssayResultReference
A Cell Based Secondary Assay To Explore Cytotoxicity of Compounds that Inhibit Mycobacterium Tuberculosis7.979 uMpubchem.aid:435019
A High Throughput Confirmatory Assay used to Identify Novel Compounds that Inhibit Mycobacterium Tuberculosis in the absence of GlycerolIC50 2.84 uMpubchem.aid:449764
A counter screen for small molecule screen for inhibitors of the PhoP regulon in Salmonella typhiIC50 2.53 uMpubchem.aid:2384
A cytotoxicity screen of small molecule inhibitors of the PhoP regulon in Salmonella typhi identified in the primary screenEC50 1.38 uMpubchem.aid:2252
A quantitative high throughput screen for small molecules that induce DNA re-replication in MCF 10a normal breast cells.18.3564 uMpubchem.aid:624296
A screen for inhibitors of the PhoP regulon in Salmonella Typhi using a modified counterscreenIC50 0.741 uMpubchem.aid:1985
A small molecule screen for inhibitors of the PhoP regulon in Salmonella typhiIC50 1.29 uMpubchem.aid:1850
ATP-based Luminescence in the Absence of Cytokines Measured in Cell-Based System Using Plate Reader - 2061-06_Inhibitor_Dose_CherryPickEC50 1.484 uMpubchem.aid:463229
AlphaScreen Assay from US Patent US9284299: "Substituted 1H-indazol-1-ol analogs as inhibitors of beta catenin/Tcf protein-protein interactions"KI 3.65 uMpubchem.aid:1802420
AlphaScreen Assay from US Patent US9284299: "Substituted 1H-indazol-1-ol analogs as inhibitors of beta catenin/Tcf protein-protein interactions"KI 3.65 uMpubchem.aid:1802420
AlphaScreen Assay from US Patent US9284299: "Substituted 1H-indazol-1-ol analogs as inhibitors of beta catenin/Tcf protein-protein interactions"KI 19.94 uMpubchem.aid:1802420
AlphaScreen Assay from US Patent US9284299: "Substituted 1H-indazol-1-ol analogs as inhibitors of beta catenin/Tcf protein-protein interactions"KI 66.23 uMpubchem.aid:1802420
AlphaScreen Assay from US Patent US9284299: "Substituted 1H-indazol-1-ol analogs as inhibitors of beta catenin/Tcf protein-protein interactions"KI 19.94 uMpubchem.aid:1802420
AlphaScreen Assay from US Patent US9284299: "Substituted 1H-indazol-1-ol analogs as inhibitors of beta catenin/Tcf protein-protein interactions"KI 66.23 uMpubchem.aid:1802420
AlphaScreen Assay from US Patent US9738628: "Substituted 1H-indazol-1-OL analogs as inhibitors of beta catenin/Tcf protein-protein interactions"KI 5.82 uMpubchem.aid:1797021
Cathepsin B dose-response confirmationIC50 0.0461 uMpubchem.aid:820
Cathepsin B mixture HTS dose-response confirmationIC50 0.04729 uMpubchem.aid:830
Cathepsin L dose-response confirmationIC50 0.22581 uMpubchem.aid:825
Cathepsin L dose-response testing in the presence of cysteineIC50 50 uMpubchem.aid:1627
Cathepsin S dose-response confirmationIC50 0.176463 uMpubchem.aid:831
Cell-based secondary assay for identifying Hsp90 inhibitors that degrade Hsp90 client protein Her2IC50 1.875 uMpubchem.aid:754
Cell-based secondary assay for identifying Hsp90 inhibitors that degrade Hsp90 client protein Her2IC50 1.875 uMpubchem.aid:754
Concentration response confirmation VP16 counterscreen for inhibitors of ROR gamma transcriptional activity0.4449 uMpubchem.aid:2763
Concentration response confirmation assay for inhibitors of ROR gamma transcriptional activity0.996 uMpubchem.aid:2762
Concentration-Response Counterscreen for Redox Active Inhibitors of Caspase-1: Catalase1.122 uMpubchem.aid:888

Elsewhere in the fleet

Open questions 2

name-disagreement — ChEBI calls this structure 'toxoflavin' and MIBiG calls it 'xanthothricin'. They share a Standard InChIKey, so if the names denote different compounds then one upstream record has the wrong structure. Check which.

shared-structure — This structure is reported by more than one MIBiG entry: BGC0000929, BGC0001972. Confirm they describe the same compound rather than an upstream cross-reference error.

Provenance

Source conceptSourceVersion
BGC0000929MIBIG3
BGC0001972MIBIG4
CHEBI:80729CHEBI3-star

This page is generated from data/natural_products/alkaloids/toxoflavin.yaml, which is generated from the committed inventories. Neither is edited by hand.