2,4-diacetylphloroglucinol
CHEBI:78688 EXACT SEEDED antifungalcytotoxicenzyme inhibitor
What these status tags mean
Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.
Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.
A benzenetriol that is phloroglucinol in which two of the ring hydrogens are replaced by acetyl groups.
Structure
| Standard InChIKey | PIFFQYJYNWXNGE-UHFFFAOYSA-N |
|---|---|
| SMILES | CC(=O)C1=C(C(=C(C=C1O)O)C(=O)C)O |
| Stereochemistry | fully defined |
| Cross-references | pubchem:16547 |
Filing pathway
Polyketides — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is pks, from MIBiG.
Producer organisms 2
A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.
| Taxon | Basis | Cluster | Evidence |
|---|---|---|---|
| Pseudomonas fluorescens NCBITaxon:294 |
BGC_CHARACTERIZED | mibig:BGC0000280 | PMID:15256586 |
| Pseudomonas fluorescens NCBITaxon:294 |
SOURCE_ASSERTION | mibig:BGC0000281 | PMID:10322017 |
Occurrences 9
Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.
| Taxon | Source | Reference |
|---|---|---|
| Pseudomonas fluorescens PF5 NCBITaxon:1218948 |
CHEBI | PMID:15826166 |
| Pseudomonas protegens PF5 NCBITaxon:220664 |
CHEBI | PMID:24742073 |
| Lysobacter gummosus NCBITaxon:262324 |
CHEBI | PMID:18058176 |
| Pseudomonas NCBITaxon:286 |
LOTUS | DOI:10.1128/AEM.00455-14 |
| Pseudomonas protegens NCBITaxon:380021 |
LOTUS | DOI:10.1094/MPMI-10-13-0311-R |
| Pseudomonas fluorescens NCBITaxon:294 |
LOTUS | DOI:10.1007/BF00633306 |
| Pseudomonas fluorescens NCBITaxon:294 |
LOTUS | DOI:10.1016/S0031-9422(00)97487-9 |
| Pseudomonas fluorescens NCBITaxon:294 |
LOTUS | DOI:10.1099/MIC.0.27033-0 |
| Pseudomonas fluorescens NCBITaxon:294 |
LOTUS | DOI:10.1128/JB.181.10.3155-3163.1999 |
Biosynthetic gene clusters 2
| Accession | Host | Locus evidence | Genome |
|---|---|---|---|
| mibig:BGC0000280 | Pseudomonas fluorescens NCBITaxon:294 |
CLUSTER_DEMONSTRATED | genbank:AF497760.1 |
| mibig:BGC0000281 | Pseudomonas fluorescens NCBITaxon:294 |
CLUSTER_UNSTATED | genbank:DQ083928.1 |
A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.
Biosynthesis curated
Written by a curator with claim-level evidence on each step; not derived from a source. This is what the rest of the record exists to support.
| Step | Enzyme | Reaction | Substrate → product | Evidence |
|---|---|---|---|---|
| 1 | PhlD UniProtKB:Q51725 |
RHEA:50380 | CHEBI:57384 → CHEBI:16204 | DOI:10.1021/ja042340g |
| 2 | PhlA/PhlC/PhlB MAPG acetylation module | CHEBI:64344 → CHEBI:78688 | DOI:10.1128/JB.181.10.3155-3163.1999 |
Step 1: Q2-87 PhlD is represented as the upstream type III polyketide synthase required for MAPG and 2,4-DAPG formation. The exact malonyl-CoA to phloroglucinol reaction is biochemically supported for the Pf-5 PhlD ortholog and marked proposed here because Bangera and Thomashow genetically placed Q2-87 PhlD upstream of MAPG before the PhlD reaction was biochemically revised.
Step 2: The step is kept as a multi-protein Q2-87 module because the 1999 paper showed PhlA, PhlC, and PhlB are collectively needed to convert MAPG to 2,4-DAPG without assigning separable individual reactions.
Mechanism & Biosynthesis Overview
Causal graph — biosynthesis 7 nodes, 6 edges
Partial Pseudomonas fluorescens Q2-87 graph for the phlACBD biosynthetic operon that converts the MAPG branch into 2,4-diacetylphloroglucinol.
| Subject | Predicate | Object | Notes | Evidence |
|---|---|---|---|---|
| PhlD | is required upstream of | monoacetylphloroglucinol | Bangera and Thomashow assigned PhlD by heterologous expression in the Q2-87 phlACBD operon; this edge captures genetic placement, not the later-refined phloroglucinol synthase reaction. | DOI:10.1128/JB.181.10.3155-3163.1999 |
| monoacetylphloroglucinol | is converted by | MAPG to 2,4-DAPG acetylation | DOI:10.1128/JB.181.10.3155-3163.1999
|
|
| PhlA | is necessary for | MAPG to 2,4-DAPG acetylation | DOI:10.1128/JB.181.10.3155-3163.1999 |
|
| PhlC | is necessary for | MAPG to 2,4-DAPG acetylation | DOI:10.1128/JB.181.10.3155-3163.1999 |
|
| PhlB | is necessary for | MAPG to 2,4-DAPG acetylation | DOI:10.1128/JB.181.10.3155-3163.1999 |
|
| MAPG to 2,4-DAPG acetylation | forms | 2,4-diacetylphloroglucinol | The exact acetylation sub-reactions are left unexpanded; the Q2-87 expression data assign PhlA, PhlC, and PhlB collectively to conversion of MAPG to 2,4-DAPG. | DOI:10.1128/JB.181.10.3155-3163.1999
|
Every edge carries its own citation; an uncited edge is refused by the corpus tests.
Causal graph — biosynthesis 6 nodes, 6 edges
In-vitro ligand binding in a 2,4-DAPG regulatory study, separated from cellular repression, pathway flux and antimicrobial action. Native references do not identify complete recombinant stocks. Supplement unread; no full-record sign-off.
| Subject | Predicate | Object | Notes | Evidence |
|---|---|---|---|---|
| Pseudomonas protegens Pf-5 | encodes_native_reference | PhlH native sequence reference | CP000076.1 CDS 6763622..6764293(-), 1-based inclusive; AAY95141.1, 223 aa. CDS-aware table-11 translation equals independent GenPept and current UniProt. Pf-5/taxon220664, unreviewed sequence version1; not Q2-87 or a complete study stock. | https://www.ncbi.nlm.nih.gov/nuccore/CP000076.1 |
| PhlH study preparation (complete sequence unverified) | has_native_sequence_reference | PhlH native sequence reference | Pf-5 gene amplified into a pET-28b-derived vector; BL21(DE3) expression. Reference correspondence, not full construct equality. | DOI:10.1128/aem.01743-23
|
| Pyoluteorin | bound_in_itc_to | PhlH study preparation (complete sequence unverified) | Figure5A: Kd1.1uM. ITC,25C,triplicates,independent binding model,blank-heat correction. No cellular occupancy or repression inferred. | DOI:10.1128/aem.01743-23
|
| Pseudomonas protegens Pf-5 | encodes_native_reference | PltZ native sequence reference | AF081920.3 CDS 22705..23376(-), 1-based inclusive; AAV66097.1, 223 aa. CDS-aware table-11 translation equals independent GenPept and current UniProt. Pf-5/taxon220664, unreviewed sequence version1; not Q2-87 or a complete study stock. | https://www.ncbi.nlm.nih.gov/nuccore/AF081920.3 |
| PltZ study preparation (complete sequence unverified) | has_native_sequence_reference | PltZ native sequence reference | Pf-5 gene amplified into a pET-28b-derived vector; BL21(DE3) expression. Reference correspondence, not full construct equality. | DOI:10.1128/aem.01743-23
|
| Pyoluteorin | bound_in_itc_to | PltZ study preparation (complete sequence unverified) | Figure5B: Kd79.2nM. ITC,25C,triplicates,independent binding model,blank-heat correction. No cellular occupancy or repression inferred. | DOI:10.1128/aem.01743-23
|
Every edge carries its own citation; an uncited edge is refused by the corpus tests.
Causal graph — biosynthesis 9 nodes, 9 edges
Pf-5 PhlG structural subgraph for 2,4-DAPG pathway homeostasis. Native reference, engineered SeMet/tagged chains and deposited assembly remain distinct. No new synthetic pathway step, kinetic constant, bound substrate or antimicrobial-resistance claim. Current structural dataset independently inspected; no full-record sign-off.
| Subject | Predicate | Object | Notes | Evidence |
|---|---|---|---|---|
| Pseudomonas protegens Pf-5 | encodes_native_reference | Native PhlG sequence reference | CP000076.1 CDS 6764435..6765319(+), 1-based inclusive; AAY95142.1,294aa. CDS-aware table-11 translation equals independent GenPept and current UniProt reviewed sequence version1, Pf-5/taxon220664. Not Q2-87 or a complete study stock. | https://www.ncbi.nlm.nih.gov/nuccore/CP000076.1 |
| 3HWP tagged SeMet PhlG chain A | has_native_sequence_reference | Native PhlG sequence reference | Canonical302aa=MGHHHHHH+native294aa only after MSE-to-M normalization; the deposited chain is tagged and selenium-substituted, not unmodified native PhlG. No complete kinetic-stock equivalence; expression metadata do not establish the full crystallization stock. | https://data.rcsb.org/rest/v1/core/polymer_entity/3HWP/1 |
| 3HWP tagged SeMet PhlG chain A | is_member_of_deposited_assembly | 3HWP deposited engineered PhlG dimer | Assembly1 contains chainsA/B under identity operation, author/PISA-defined. Deposited dimer membership is not universal physiological stoichiometry. | https://data.rcsb.org/rest/v1/core/assembly/3HWP/1 |
| 3HWP zinc in author chain A residue295 | is_deposited_coordinated_by | 3HWP tagged SeMet PhlG chain A | Four protein ligands in author numbering: H129NE2,E160OE1,H270NE2,E274OE1. Coordinates and all metal links checked. Not direct evidence of a catalytic transition state. | https://files.rcsb.org/download/3HWP.cif
|
| 3HWP water in author chain A residue307 | is_deposited_coordinated_to | 3HWP zinc in author chain A residue295 | A modeled water contact, not an observed nucleophilic attack or bound DAPG/MAPG intermediate. | https://files.rcsb.org/download/3HWP.cif
|
| 3HWP tagged SeMet PhlG chain B | has_native_sequence_reference | Native PhlG sequence reference | Canonical302aa=MGHHHHHH+native294aa only after MSE-to-M normalization; the deposited chain is tagged and selenium-substituted, not unmodified native PhlG. No complete kinetic-stock equivalence; expression metadata do not establish the full crystallization stock. | https://data.rcsb.org/rest/v1/core/polymer_entity/3HWP/1 |
| 3HWP tagged SeMet PhlG chain B | is_member_of_deposited_assembly | 3HWP deposited engineered PhlG dimer | Assembly1 contains chainsA/B under identity operation, author/PISA-defined. Deposited dimer membership is not universal physiological stoichiometry. | https://data.rcsb.org/rest/v1/core/assembly/3HWP/1 |
| 3HWP zinc in author chain B residue295 | is_deposited_coordinated_by | 3HWP tagged SeMet PhlG chain B | Four protein ligands in author numbering: H129NE2,E160OE1,H270NE2,E274OE1. Coordinates and all metal links checked. Not direct evidence of a catalytic transition state. | https://files.rcsb.org/download/3HWP.cif
|
| 3HWP water in author chain B residue297 | is_deposited_coordinated_to | 3HWP zinc in author chain B residue295 | A modeled water contact, not an observed nucleophilic attack or bound DAPG/MAPG intermediate. | https://files.rcsb.org/download/3HWP.cif
|
Every edge carries its own citation; an uncited edge is refused by the corpus tests.
Causal graph — biosynthesis 16 nodes, 18 edges
Pf-5 PhlD purified-enzyme and combined phlACB expression observations from the2005 primary abstract, with four current native sequence references. Native references, unresolved historical constructs and study preparations remain distinct. Existing Q2-87 pathway not reassigned. No complete biosynthetic sequence, individual PhlA/B/C catalysis, native complex, producer-grade upgrade or antimicrobial-target assertion. Main/figures/SI unread; no full-record sign-off.
| Subject | Predicate | Object | Notes | Evidence |
|---|---|---|---|---|
| Pseudomonas protegens Pf-5 | encodes_native_reference | PhlA native Pf-5 sequence reference | CP000076.1 CDS 6766441..6767523(+),1-based inclusive; AAY95144.2/PFL_5954,360aa. CDS-aware table11 translation equals independent GenPept and current UniProt sequencev2. Pf-5/taxon220664, not the existing Q2-87 pathway proteins. Current native reference, not full historical construct identity. PhlA sequencev2 was revised2011-10-19, after the2005 study. | https://www.ncbi.nlm.nih.gov/nuccore/CP000076.1 |
| Study phlA expression construct (sequence unverified) | has_native_coding_locus_reference | PhlA native Pf-5 sequence reference | Reported Pf-5 gene name maps to the current native reference; exact study allele, full construct and translated stock sequence are not established. Current PhlA sequence revision postdates the study. | DOI:10.1021/ja042340g
|
| Study E. coli expressing Pf-5 phlACB | expresses_reported_gene_construct | Study phlA expression construct (sequence unverified) | The abstract reports phlACB expression together in E. coli; this identifies the experimental context, not individual protein detection, purified subunit activity, necessity, complex membership or subunit stoichiometry. | DOI:10.1021/ja042340g
|
| Pseudomonas protegens Pf-5 | encodes_native_reference | PhlB native Pf-5 sequence reference | CP000076.1 CDS 6768762..6769202(+),1-based inclusive; AAY95146.1/PFL_5956,146aa. CDS-aware table11 translation equals independent GenPept and current UniProt sequencev1. Pf-5/taxon220664, not the existing Q2-87 pathway proteins. Current native reference, not full historical construct identity. | https://www.ncbi.nlm.nih.gov/nuccore/CP000076.1 |
| Study phlB expression construct (sequence unverified) | has_native_coding_locus_reference | PhlB native Pf-5 sequence reference | Reported Pf-5 gene name maps to the current native reference; exact study allele, full construct and translated stock sequence are not established. Not a Q2-87 protein. | DOI:10.1021/ja042340g
|
| Study E. coli expressing Pf-5 phlACB | expresses_reported_gene_construct | Study phlB expression construct (sequence unverified) | The abstract reports phlACB expression together in E. coli; this identifies the experimental context, not individual protein detection, purified subunit activity, necessity, complex membership or subunit stoichiometry. | DOI:10.1021/ja042340g
|
| Pseudomonas protegens Pf-5 | encodes_native_reference | PhlC native Pf-5 sequence reference | CP000076.1 CDS 6767553..6768749(+),1-based inclusive; AAY95145.1/PFL_5955,398aa. CDS-aware table11 translation equals independent GenPept and current UniProt sequencev1. Pf-5/taxon220664, not the existing Q2-87 pathway proteins. Current native reference, not full historical construct identity. | https://www.ncbi.nlm.nih.gov/nuccore/CP000076.1 |
| Study phlC expression construct (sequence unverified) | has_native_coding_locus_reference | PhlC native Pf-5 sequence reference | Reported Pf-5 gene name maps to the current native reference; exact study allele, full construct and translated stock sequence are not established. Not a Q2-87 protein. | DOI:10.1021/ja042340g
|
| Study E. coli expressing Pf-5 phlACB | expresses_reported_gene_construct | Study phlC expression construct (sequence unverified) | The abstract reports phlACB expression together in E. coli; this identifies the experimental context, not individual protein detection, purified subunit activity, necessity, complex membership or subunit stoichiometry. | DOI:10.1021/ja042340g
|
| Pseudomonas protegens Pf-5 | encodes_native_reference | PhlD native Pf-5 sequence reference | CP000076.1 CDS 6769411..6770460(+),1-based inclusive; AAY95147.1/PFL_5957,349aa. CDS-aware table11 translation equals independent GenPept and current UniProt sequencev1. Pf-5/taxon220664, not the existing Q2-87 pathway proteins. Current native reference, not full historical construct identity. | https://www.ncbi.nlm.nih.gov/nuccore/CP000076.1 |
| Purified study PhlD (complete preparation unverified) | has_native_sequence_reference | PhlD native Pf-5 sequence reference | The abstract names Pf-5 PhlD. Mapping to the current Pf-5 reference is an explicit gene/strain inference, not a reconstructed purification stock or complete engineered sequence. | DOI:10.1021/ja042340g
|
| Purified study PhlD (complete preparation unverified) | catalyzes_reported_conversion | Reported PhlD conversion of malonyl-CoA to phloroglucinol | Purification to homogeneity and substrate-to-product conversion are reported in the primary abstract. Kinetic numbers are omitted because complete assay conditions were not inspected. This is not direct DAPG synthesis or a claim about the Q2-87 ortholog. | DOI:10.1021/ja042340g
|
| Source-assigned malonyl-CoA substrate | is_reported_substrate_of | Reported PhlD conversion of malonyl-CoA to phloroglucinol | Substrate identity as reported; no molar stoichiometry, intermediates, cofactors or reaction balance inferred from the abstract. | DOI:10.1021/ja042340g
|
| Reported PhlD conversion of malonyl-CoA to phloroglucinol | yields_reported_product | Source-assigned phloroglucinol | Phloroglucinol is the reported purified-enzyme product; it is not MAPG or DAPG. | DOI:10.1021/ja042340g
|
| Study E. coli expressing Pf-5 phlACB | supports_reported_interconversions | Reported phlACB-associated PG/MAPG/DAPG interconversions | Acetylating and deacetylating activities were reported with phlACB expressed in E. coli. The abstract does not resolve individual subunit catalysis, preparation format, physiological direction, quantitative flux or a complete native complex. | DOI:10.1021/ja042340g
|
| Source-assigned phloroglucinol | participates_in_reported_interconversions | Reported phlACB-associated PG/MAPG/DAPG interconversions | One of the three source-assigned compounds in the combined phlACB expression result. The abstract does not establish each pairwise direct reaction, equilibrium, stoichiometry or a native whole-cell production rate. | DOI:10.1021/ja042340g
|
| monoacetylphloroglucinol | participates_in_reported_interconversions | Reported phlACB-associated PG/MAPG/DAPG interconversions | One of the three source-assigned compounds in the combined phlACB expression result. The abstract does not establish each pairwise direct reaction, equilibrium, stoichiometry or a native whole-cell production rate. | DOI:10.1021/ja042340g
|
| 2,4-Diacetylphloroglucinol | participates_in_reported_interconversions | Reported phlACB-associated PG/MAPG/DAPG interconversions | One of the three source-assigned compounds in the combined phlACB expression result. The abstract does not establish each pairwise direct reaction, equilibrium, stoichiometry or a native whole-cell production rate. | DOI:10.1021/ja042340g
|
Every edge carries its own citation; an uncited edge is refused by the corpus tests.
Causal graph — biosynthesis 7 nodes, 8 edges
Bounded Pf-5 GacA genetics in a DAPG regulatory study. Native references, reporter-bearing cultures and GFP readouts remain distinct. RNA-probe assignments, antimicrobial phenotypes and full-record sign-off are excluded.
| Subject | Predicate | Object | Notes | Evidence |
|---|---|---|---|---|
| Pseudomonas protegens Pf-5 | encodes_native_reference | Native GacA sequence reference | CP000076.1 CDS4110513..4111154(-), 1-based inclusive; PFL_3563, AAY92829.1,213aa. CDS-aware table11 translation equals GenPept and UniProt sequence version1, unreviewed, taxon220664. Historical P.fluorescens display name retains the exact Pf-5 strain. Not a complete engineered stock or a protein asserted present in the mutant. | https://www.ncbi.nlm.nih.gov/nuccore/CP000076.1 |
| JL4975-derived reporter culture | has_reported_deletion_of_coding_locus_for | Native GacA sequence reference | Unmarked in-frame gacA deletion; exact junctions and residual product unverified. | DOI:10.1128/aem.01546-25
|
| LK099-derived reporter culture | has_native_background_reference | Pseudomonas protegens Pf-5 | LK099 background with reporter, not an unmodified genome-equivalent stock. | DOI:10.1128/aem.01546-25
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| JL4975-derived reporter culture | carries_reporter | Study phlD translation GFP reporter | pPROBE-NT derivative; full plasmid sequence unverified. | DOI:10.1128/aem.01546-25
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| LK099-derived reporter culture | carries_reporter | Study phlD translation GFP reporter | Promoter and six phlD codons fused to GFP; no native protein abundance measurement. | DOI:10.1128/aem.01546-25
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| JL4975-derived reporter culture | yielded | Mutant normalized GFP readout | PDB,28C; fluorescence normalized to OD600 after empty-vector correction. | DOI:10.1128/aem.01546-25
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| LK099-derived reporter culture | yielded | Control normalized GFP readout | Matched reporter comparison at10,16,24h; Figure3A mean and SE, n=3. | DOI:10.1128/aem.01546-25
|
| Mutant normalized GFP readout | was_lower_than | Control normalized GFP readout | Clear separation at16/24h; no numerical fold change or significance at10h asserted. Not direct DNA binding, isolated translation rate, DAPG flux or RsmE independence. | DOI:10.1128/aem.01546-25
|
Every edge carries its own citation; an uncited edge is refused by the corpus tests.
Causal graph — biosynthesis 5 nodes, 5 edges
Independent apo PhlH structural subgraph from the DAPG regulatory study associated with DOI:10.1016/j.jbc.2022.102027. No bound DAPG or DNA in this model. The companion7E1N ligand-identity disagreement is not resolved or adopted here. Versioned nucleotide accession is cited as sequence evidence, not assigned as a BGC identifier. Primary abstract only; main text, images and SI unread. No full-record sign-off.
| Subject | Predicate | Object | Notes | Evidence |
|---|---|---|---|---|
| Native 2P24 phl locus (sequence DQ083928.1) | encodes_native_reference | Native 2P24 PhlH sequence reference | DQ083928.1 CDS1..678(-),1-based inclusive,AAY86545.1,225aa. CDS-aware table11=GenPept=UniProt sequencev1. Native strain2P24/taxon294 comes from GenBank/GenPept; UniProt is species-level and unreviewed. Not Pf-5 or CHA0 PhlH. | https://www.ncbi.nlm.nih.gov/nuccore/DQ083928.1 |
| 7E1L tagged SeMet PhlH chain A | has_native_sequence_reference | Native 2P24 PhlH sequence reference | Canonical234aa=MGHHHHHHH+native225aa only after MSE-to-M normalization. Crystal source strain unrecorded; complete stock and vector unverified. Reference correspondence is not unmodified native-protein identity. | https://data.rcsb.org/rest/v1/core/polymer_entity/7E1L/1 |
| 7E1L tagged SeMet PhlH chain A | is_member_of_deposited_assembly | 7E1L deposited tagged SeMet PhlH dimer | Author/PISA assembly1:chainsA/B under identity operation; depositor reports gel filtration. Model1.2 revised2024-11-13,2.4A. No universal physiological stoichiometry, ligand-triggered movement or DNA-binding activity inferred. | https://data.rcsb.org/rest/v1/core/assembly/7E1L/1 |
| 7E1L tagged SeMet PhlH chain B | has_native_sequence_reference | Native 2P24 PhlH sequence reference | Canonical234aa=MGHHHHHHH+native225aa only after MSE-to-M normalization. Crystal source strain unrecorded; complete stock and vector unverified. Reference correspondence is not unmodified native-protein identity. | https://data.rcsb.org/rest/v1/core/polymer_entity/7E1L/1 |
| 7E1L tagged SeMet PhlH chain B | is_member_of_deposited_assembly | 7E1L deposited tagged SeMet PhlH dimer | Author/PISA assembly1:chainsA/B under identity operation; depositor reports gel filtration. Model1.2 revised2024-11-13,2.4A. No universal physiological stoichiometry, ligand-triggered movement or DNA-binding activity inferred. | https://data.rcsb.org/rest/v1/core/assembly/7E1L/1 |
Every edge carries its own citation; an uncited edge is refused by the corpus tests.
Causal graph — biosynthesis 9 nodes, 9 edges
Bounded RsmE assay evidence in a Pf-5 DAPG regulatory study. Native reference, tagged preparation, author-labelled probes and signals remain separate. Probe identities require review; no exact native RNA binding edge, antimicrobial mechanism or full-record sign-off.
| Subject | Predicate | Object | Notes | Evidence |
|---|---|---|---|---|
| Pseudomonas protegens Pf-5 | encodes_native_reference | Native RsmE sequence reference | CP000076.1 CDS2317318..2317512(-),1-based inclusive;PFL_2095, AAY91370.1,64aa. CDS-aware table11=GenPept=UniProt,unreviewed sequencev1,taxon220664. Generic UniProt CsrA protein name does not override the rsmE gene/locus/exact sequence mapping. | https://www.ncbi.nlm.nih.gov/nuccore/CP000076.1 |
| Study His-RsmE preparation | has_native_sequence_reference | Native RsmE sequence reference | Gene-matched native reference, not complete tagged-stock identity. Full expression construct and exact tag junction remain unverified. | DOI:10.1128/aem.01546-25
|
| Study His-RsmE preparation | was_tested_in | phlA-labelled probe AlphaScreen assay | Figure3B:RsmE5nM;probe1,5,10,50nM. Assay concentrations are not binding stoichiometry. No affinity or fitted Kd asserted. | DOI:10.1128/aem.01546-25
|
| Author-labelled phlA leader mRNA probe | was_tested_in | phlA-labelled probe AlphaScreen assay | Author assay label, not verified native RNA identity. Table1 primer comparison with CP000076.1 places the longest exact 3prime suffix of KpnI-phlA_F (21nt,6769410..6769430+) and PhlA_UTRT7_R (20nt,6769421..6769440-) at phlD, not phlA. Two other reported primers lack an exact terminal match of at least15nt. This is a documentation/construct ambiguity, not proof that the actual reagent was wrong; full plasmids and probes remain unverified. | DOI:10.1128/aem.01546-25 |
| phlA-labelled probe AlphaScreen assay | yielded | phlA-labelled probe AlphaScreen signal | Retained Figure3B readout only; no digitized numeric count, replicate estimate or downstream translation effect adopted. | DOI:10.1128/aem.01546-25
|
| Study His-RsmE preparation | was_tested_in | phlD-labelled probe AlphaScreen assay | Figure3B:RsmE5nM;probe1,5,10,50nM. Assay concentrations are not binding stoichiometry. No affinity or fitted Kd asserted. | DOI:10.1128/aem.01546-25
|
| Author-labelled phlD leader mRNA probe | was_tested_in | phlD-labelled probe AlphaScreen assay | Author assay label, not verified native RNA identity. Table1 primer comparison with CP000076.1 places the longest exact 3prime suffix of KpnI-phlA_F (21nt,6769410..6769430+) and PhlA_UTRT7_R (20nt,6769421..6769440-) at phlD, not phlA. Two other reported primers lack an exact terminal match of at least15nt. This is a documentation/construct ambiguity, not proof that the actual reagent was wrong; full plasmids and probes remain unverified. | DOI:10.1128/aem.01546-25 |
| phlD-labelled probe AlphaScreen assay | yielded | phlD-labelled probe AlphaScreen signal | Retained Figure3B readout only; no digitized numeric count, replicate estimate or downstream translation effect adopted. | DOI:10.1128/aem.01546-25
|
| phlA-labelled probe AlphaScreen signal | was_higher_than | phlD-labelled probe AlphaScreen signal | Qualitative separation at5/10nM author-labelled probes; phlD-labelled trace remains low. No exact native RNA binding, universal nonbinding, physiological repression, DAPG flux or GacA-RsmE causal chain asserted. Lower signals at50nM are not interpreted as a change in affinity. | DOI:10.1128/aem.01546-25
|
Every edge carries its own citation; an uncited edge is refused by the corpus tests.
Causal graph — biosynthesis 7 nodes, 6 edges
F113 phlE-associated production genetics only. The reported cell-associated/free-PHL ratio did not differ significantly; direct or exclusive export is not established. No resistance, stress-tolerance or antimicrobial mechanism copied from the paper. Native reference, mutant allele and study cultures remain distinct; no producer upgrade or full-record sign-off.
| Subject | Predicate | Object | Notes | Evidence |
|---|---|---|---|---|
| F113 native sequence source | encodes_native_reference | PhlE native F113 sequence reference | AJ542662.1 CDS1..1272(+),1-based inclusive,CAD65845.1,423aa. CDS-aware table11=GenPept=UniProt Q845L9,unreviewed entry57/sequence1. Native strain F113 from GenBank/GenPept; species-level taxon294,not a strain taxid. Not Pf-5,CHA0,Q2-87 or an intact protein in F113-D3. | https://www.ncbi.nlm.nih.gov/nuccore/AJ542662.1 |
| Study phlE null allele (lesion unresolved) | affects_coding_locus_of | PhlE native F113 sequence reference | Gene/strain correspondence, not resolved mutation boundaries or proof excluding polar effects. | DOI:10.1099/mic.0.27033-0
|
| Study phlE null allele (lesion unresolved) | was_present_in | Study F113-D3 phlE mutant culture | Abstract names the phlE null mutant F113-D3; no intact mutant PhlE sequence inferred. | DOI:10.1099/mic.0.27033-0
|
| Study F113 reference culture | is_reference_background_for | Reported F113-D3 versus F113 production comparison | Named comparator; full culture protocol and historical stock unverified. | DOI:10.1099/mic.0.27033-0
|
| Study F113-D3 phlE mutant culture | showed_lower_production_in | Reported F113-D3 versus F113 production comparison | Qualitative production phenotype, not a transport-rate, growth-normalized flux or direct catalytic assignment. | DOI:10.1099/mic.0.27033-0
|
| Reported F113-D3 versus F113 production comparison | measured_reported_product | 2,4-diacetylphloroglucinol | PHL denotes 2,4-DAPG in the abstract. No quantitative titer, extraction procedure or reaction step adopted. | DOI:10.1099/mic.0.27033-0
|
Every edge carries its own citation; an uncited edge is refused by the corpus tests.
Causal graph — biosynthesis 11 nodes, 11 edges
Independent structural dataset for DAPG-biosynthesis-associated PhlABC. Deposited participants are separate from native CHA0/Pf-5 sequence references; PhlA source-taxonomy disagreement remains REVIEW_NEEDED. Assembly multiplicity, PhlB zinc contacts and PhlC SCY88 are structural observations, not turnover, native mixed-strain complex or physiological stoichiometry. No bound DAPG/MAPG, complete biosynthesis, producer-grade upgrade or antimicrobial-target claim. Associated paper/abstract/figures/SI unread; not full-record sign-off.
| Subject | Predicate | Object | Notes | Evidence |
|---|---|---|---|---|
| Native CHA0 source (conflicting species annotations) | encodes_native_sequence_reference | PhlA CHA0 sequence reference (taxonomy discrepancy) | AF207529.2 CDS30..1118(-),1-based inclusive; AAF20927.1,362aa. CDS-aware table11 translation equals independent GenPept and UniProtQ9TP23 sequencev1. GenBank/GenPept identify CHA0 as P.protegens/taxon380021; UniProt and the deposit use P.fluorescens/taxon294. Taxonomy discrepancy is explicit, not silently reconciled. Q9TP23 has CHA0 reference metadata; the deposit lacks a PhlA strain qualifier. This does not establish exact cloning source. PhlA remains REVIEW_NEEDED for the conflicting organism annotations. | https://www.ncbi.nlm.nih.gov/nuccore/AF207529.2 |
| Pseudomonas protegens Pf-5 | encodes_native_sequence_reference | PhlB native Pf-5 reference | CP000076.1 CDS6768762..6769202(+),1-based inclusive; AAY95146.1,146aa. CDS-aware table11 translation equals independent GenPept and current UniProt. Native Pf-5/taxon220664 reference, not the complete engineered stock, a Q2-87 protein or proof of the PhlA cloning source. | https://www.ncbi.nlm.nih.gov/nuccore/CP000076.1 |
| Pseudomonas protegens Pf-5 | encodes_native_sequence_reference | PhlC native Pf-5 reference | CP000076.1 CDS6767553..6768749(+),1-based inclusive; AAY95145.1,398aa. CDS-aware table11 translation equals independent GenPept and current UniProt. Native Pf-5/taxon220664 reference, not the complete engineered stock, a Q2-87 protein or proof of the PhlA cloning source. | https://www.ncbi.nlm.nih.gov/nuccore/CP000076.1 |
| 5M3K deposited phla participant, chains A/D | has_native_sequence_reference | PhlA CHA0 sequence reference (taxonomy discrepancy) | The deposited362aa sequence equals CHA0 referenceQ9TP23, not Pf-5Q4K421/360aa. Source metadata give P.fluorescens/taxon294 but no strain; exact clone source and complete stock unverified. | https://data.rcsb.org/rest/v1/core/polymer_entity/5M3K/1 |
| 5M3K deposited phla participant, chains A/D | is_member_of_deposited_assembly | 5M3K deposited author/PISA assembly1 | Assembly1 uses operations1,2 on the six ASU polymer chains A-F, giving 12 polymer instances, four of each PhlA/PhlB/PhlC. The six-chain ASU is not the deposited assembly. Genetically engineered sources and E.coli/taxon562 expression host do not establish native assembly or host DAPG production. | https://data.rcsb.org/rest/v1/core/assembly/5M3K/1 |
| 5M3K deposited phlb participant, chains B/E | has_native_sequence_reference | PhlB native Pf-5 reference | Deposited146aa sequence equals nativePf-5Q4K419. Deposit sourcePf-5/taxon220664; sequence equality does not establish the complete recombinant preparation. | https://data.rcsb.org/rest/v1/core/polymer_entity/5M3K/2 |
| 5M3K deposited phlb participant, chains B/E | is_member_of_deposited_assembly | 5M3K deposited author/PISA assembly1 | Assembly1 uses operations1,2 on the six ASU polymer chains A-F, giving 12 polymer instances, four of each PhlA/PhlB/PhlC. The six-chain ASU is not the deposited assembly. Genetically engineered sources and E.coli/taxon562 expression host do not establish native assembly or host DAPG production. | https://data.rcsb.org/rest/v1/core/assembly/5M3K/1 |
| 5M3K deposited phlc participant, chains C/F | has_native_sequence_reference | PhlC native Pf-5 reference | Canonical398aa sequence equals nativePf-5Q4K420 only after SCY88-to-C normalization. Raw deposited sequence contains S-acetyl-cysteine at88, not an unmodified native cysteine. Deposit sourcePf-5/taxon220664. | https://data.rcsb.org/rest/v1/core/polymer_entity/5M3K/3 |
| 5M3K deposited phlc participant, chains C/F | is_member_of_deposited_assembly | 5M3K deposited author/PISA assembly1 | Assembly1 uses operations1,2 on the six ASU polymer chains A-F, giving 12 polymer instances, four of each PhlA/PhlB/PhlC. The six-chain ASU is not the deposited assembly. Genetically engineered sources and E.coli/taxon562 expression host do not establish native assembly or host DAPG production. | https://data.rcsb.org/rest/v1/core/assembly/5M3K/1 |
| Deposited zinc sites adjacent to PhlB chains B/E | has_deposited_coordination_contacts_to | 5M3K deposited phlb participant, chains B/E | Eight metal-contact rows: PhlB Cys34/37/48/51 SG in chainB contacts Zn labelG/authorB200; the corresponding four cysteines in chainE contact Zn labelH/authorE200. All deposited distances agree with atom coordinates within0.002A. These are model coordination contacts, not a kinetic metal requirement. | https://files.rcsb.org/download/5M3K.cif
|
| Deposited S-acetyl-cysteine88 in PhlC chains C/F | is_deposited_modified_residue_in | 5M3K deposited phlc participant, chains C/F | SCY88 is present in both PhlC chainsC/F with CYS as parent component. The four covalent rows are same-chain adjacent peptide87-88 and88-89 links, not protein-protein crosslinks. Modification alone does not prove an on-pathway physiological intermediate, acetyl donor or catalytic mechanism. | https://files.rcsb.org/download/5M3K.cif
|
Every edge carries its own citation; an uncited edge is refused by the corpus tests.
Causal graph — biosynthesis 8 nodes, 7 edges
CHA0 pathway-associated DAPG turnover, not a synthesis step: a native PhlG reference is distinct from the tagged E.coli-expressed preparation. REVIEW_NEEDED retains differing307aa and294aa native reference annotations. Evidence is bounded to the primary abstract and captions. No whole-cell flux, universal specificity, pyoluteorin binding mechanism or antimicrobial target asserted. Main/images/tables/SI unread; no producer-grade upgrade or full-record sign-off.
| Subject | Predicate | Object | Notes | Evidence |
|---|---|---|---|---|
| Native CHA0 reference source | encodes_native_reference | PhlG AF207529.2 CHA0 sequence reference (variant unresolved) | AF207529.2 CDS2234..3157(-),1-based inclusive; AAF20929.2,307aa. CDS-aware table11 translation equals independent GenPept and current UniProt. Native sourceCHA0,not Pf-5 or Q2-87; no complete historical assay-stock sequence inferred. UniProt primaryA0A2C9EVE6 has secondaryQ9RF01 and strain taxon1124983; GenBank/GenPept use species taxon380021. Sequencev1 updated2017-12-20 does not establish the complete2006 preparation or tag sequence. Revised CHA0 genome LS999205.1 CDS6558372..6559256(+), VAV72158.1, encodes294aa, exactly residues14..307 of this307aa reference; the13aa N-terminal difference does not identify the historical assay start site. REVIEW_NEEDED retains reference-version/strain-stock ambiguity. No source is silently replaced; no engineered mutation or biological cause of these sequence differences is inferred. | https://www.ncbi.nlm.nih.gov/nuccore/AF207529.2 |
| Study purified PhlG-His6 (complete construct unresolved) | has_native_sequence_reference | PhlG AF207529.2 CHA0 sequence reference (variant unresolved) | The paper names CHA0 PhlG; AF207529.2 reference selected by gene/strain. Full recombinant sequence, tag terminus/linker and exact historical stock not established. The307aa AF207529.2/UniProt reference differs from the294aa revised-genome annotation; neither is established as the complete His-tagged preparation. | DOI:10.1128/AEM.72.1.418-427.2006 |
| Study E. coli BL21(DE3)/pME8032 expression context | was_expression_context_for | Study purified PhlG-His6 (complete construct unresolved) | Figure4 caption identifies BL21(DE3)/pME8032 and the purified His6-labeled preparation. Expression host is not a native DAPG producer assertion. | DOI:10.1128/AEM.72.1.418-427.2006
|
| Study purified PhlG-His6 (complete construct unresolved) | catalyzes_reported_degradation | Reported PhlG-His6 DAPG degradation assay | Purified tagged-enzyme conversion reported in the abstract; Figure5 caption describes an HPLC time course. No kinetic constants or complete buffer composition imported from the abstract or caption alone. | DOI:10.1128/AEM.72.1.418-427.2006
|
| 2,4-diacetylphloroglucinol | is_reported_substrate_of | Reported PhlG-His6 DAPG degradation assay | Exact named assay substrate; not a claim about every DAPG protonation state, intracellular concentration, native flux or biosynthetic direction. | DOI:10.1128/AEM.72.1.418-427.2006
|
| Reported PhlG-His6 DAPG degradation assay | yields_reported_product | monoacetylphloroglucinol | MAPG is the reported degradation product, not phloroglucinol. No further MAPG turnover or universal substrate specificity inferred. | DOI:10.1128/AEM.72.1.418-427.2006
|
| Reported PhlG-His6 DAPG degradation assay | yields_reported_product | Source-assigned acetate product | Acetate is reported with MAPG in the primary abstract. No independent analytical identity procedure or balanced reaction is reconstructed here. | DOI:10.1128/AEM.72.1.418-427.2006
|
Every edge carries its own citation; an uncited edge is refused by the corpus tests.
Causal graph — biosynthesis 12 nodes, 12 edges
Separate CHA0 reporter comparisons for PhlF and PhlH, not direct DNA binding or enzyme assays. Native references retain REVIEW_NEEDED species-label and revised-genome sequence discrepancies; unresolved alleles and study backgrounds remain distinct. No exact lesion, quantitative fold change, GacS/GacA protein assignment or producer-grade upgrade. Main/images/tables/SI unread; no full-record sign-off.
| Subject | Predicate | Object | Notes | Evidence |
|---|---|---|---|---|
| Study CHA0 reference reporter background | is_reference_background_for | Separate phlF and phlH reporter comparisons | Figure7 caption identifies wild-typeCHA0 as the reference. Separate mutant comparisons, not one culture carrying both mutations. | DOI:10.1128/AEM.72.1.418-427.2006
|
| Separate phlF and phlH reporter comparisons | uses_reporter_readout | pME8030 phlG-lacZ translational-fusion readout | Figure7 caption reports beta-galactosidase activity from pME8030 in OSGly-grown cultures. A translational-fusion signal is not direct promoter occupancy, isolated transcription rate or purified PhlG activity. | DOI:10.1128/AEM.72.1.418-427.2006
|
| Native CHA0 source (species annotation discrepancy) | encodes_native_reference | PhlF AF207529.2 CHA0 sequence reference (variant unresolved) | AF207529.2 CDS1580..2182(+),1-based inclusive; AAF20928.1,200aa. CDS-aware table11 translation equals independent GenPept and current UniProt. Native sourceCHA0,not Pf-5 or Q2-87; no complete historical assay-stock sequence inferred. GenBank/GenPept label CHA0 P.protegens/taxon380021; UniProt labels P.fluorescens/taxon294. Revised CHA0 genome LS999205.1 CDS6559308..6559910(-), VAV72159.1, differs at188: this referenceF versus genomeS. REVIEW_NEEDED retains reference-version/strain-stock ambiguity. No source is silently replaced; no engineered mutation or biological cause of these sequence differences is inferred. | https://www.ncbi.nlm.nih.gov/nuccore/AF207529.2 |
| Study phlF mutant allele (lesion unresolved) | affects_coding_locus_of | PhlF AF207529.2 CHA0 sequence reference (variant unresolved) | Gene-name correspondence only, not a resolved allele, intact mutant protein, in-frame deletion or proof excluding polar effects. | DOI:10.1128/AEM.72.1.418-427.2006
|
| Study phlF mutant allele (lesion unresolved) | was_present_in | Study CHA638 phlF reporter background | Figure7 caption identifies the named mutant background; exact lesion and complete stock unresolved. | DOI:10.1128/AEM.72.1.418-427.2006
|
| Study CHA638 phlF reporter background | is_perturbed_background_for | Separate phlF and phlH reporter comparisons | Reported mutant reporter context, kept separate from the native reference sequence and the purified-enzyme experiment. | DOI:10.1128/AEM.72.1.418-427.2006
|
| Separate phlF and phlH reporter comparisons | supports_proposed | Authors' inferred phlF-associated negative control | Abstract reports negative control by this pathway-specific regulator; Figure7 caption identifies the reporter comparison. No fold change, direct DNA binding, promoter site, effector ligand or isolated regulatory complex. | DOI:10.1128/AEM.72.1.418-427.2006
|
| Native CHA0 source (species annotation discrepancy) | encodes_native_reference | PhlH AF207529.2 CHA0 sequence reference (variant unresolved) | AF207529.2 CDS3260..3931(+),1-based inclusive; AAF20930.1,223aa. CDS-aware table11 translation equals independent GenPept and current UniProt. Native sourceCHA0,not Pf-5 or Q2-87; no complete historical assay-stock sequence inferred. GenBank/GenPept label CHA0 P.protegens/taxon380021; UniProt labels P.fluorescens/taxon294. Revised CHA0 genome LS999205.1 CDS6557559..6558230(-), VAV72157.1, differs at215 and217: this referenceS/M versus genomeA/V. REVIEW_NEEDED retains reference-version/strain-stock ambiguity. No source is silently replaced; no engineered mutation or biological cause of these sequence differences is inferred. | https://www.ncbi.nlm.nih.gov/nuccore/AF207529.2 |
| Study phlH mutant allele (lesion unresolved) | affects_coding_locus_of | PhlH AF207529.2 CHA0 sequence reference (variant unresolved) | Gene-name correspondence only, not a resolved allele, intact mutant protein, in-frame deletion or proof excluding polar effects. | DOI:10.1128/AEM.72.1.418-427.2006
|
| Study phlH mutant allele (lesion unresolved) | was_present_in | Study CHA630 phlH reporter background | Figure7 caption identifies the named mutant background; exact lesion and complete stock unresolved. | DOI:10.1128/AEM.72.1.418-427.2006
|
| Study CHA630 phlH reporter background | is_perturbed_background_for | Separate phlF and phlH reporter comparisons | Reported mutant reporter context, kept separate from the native reference sequence and the purified-enzyme experiment. | DOI:10.1128/AEM.72.1.418-427.2006
|
| Separate phlF and phlH reporter comparisons | supports_proposed | Authors' inferred phlH-associated negative control | Abstract reports negative control by this pathway-specific regulator; Figure7 caption identifies the reporter comparison. No fold change, direct DNA binding, promoter site, effector ligand or isolated regulatory complex. | DOI:10.1128/AEM.72.1.418-427.2006
|
Every edge carries its own citation; an uncited edge is refused by the corpus tests.
Bioactivities 25
Entries preserve source qualifiers, targets and evidence. Repeated assay names or values are not independent replications; inspect source detail for differences.
| Assay | Result | Target enzyme | Source detail |
|---|---|---|---|
| A quantitative high throughput screen for small molecules that induce DNA re-replication in SW480 colon adenocarcinoma cells. | OTHER 1.4581 uM; NOT_DETERMINED | Not recorded | pubchem.aid:624297
|
| Counterscreen for agonists of nuclear receptor subfamily 2, group E, member 3 (NR2E3):Luminescence-based cell-based high throughput dose response assay to identify inhibitors of the Herpes Virus Virion Protein 16 (VP16) | IC50 4.561 uM; ACTIVE | Not recorded | pubchem.aid:624395
|
| High Throughput Screening for Foot and Mouth Disease Virus Antivirals | OTHER 35.4813 uM; NOT_DETERMINED | Not recorded | pubchem.aid:1159524
|
| Luminescence Microorganism-Based Dose Confirmation HTS to Identify Compounds Cytotoxic to SK(-)GAS Group A Streptococcus | EC50 3.897 uM; ACTIVE | Not recorded | pubchem.aid:1900
|
| Luminescence Microorganism-Based Dose Confirmation HTS to Identify Inhibitors of Streptokinase Promotor Activity | EC50 2.635 uM; ACTIVE | Not recorded | pubchem.aid:1902
|
| Luminescence Microorganism-Based Dose Response HTS to Identify Compounds Cytotoxic to Streptococcus | EC50 3.976 uM; ACTIVE | Not recorded | pubchem.aid:1915
|
| Luminescence-based cell-based high throughput dose response assay for agonists of nuclear receptor subfamily 2, group E, member 3 (NR2E3) | IC50 3.238 uM; ACTIVE | Not recorded | pubchem.aid:624394
|
| Nrf2 qHTS screen for inhibitors | OTHER 25.929 uM; NOT_DETERMINED | Not recorded | pubchem.aid:504444
|
| Primary qHTS for delayed death inhibitors of the malarial parasite plastid, 48 hour incubation | OTHER 10.4179 uM; ACTIVE | Not recorded | pubchem.aid:504832
|
| VP16 counterscreen qHTS for inhibitors of ROR gamma transcriptional activity | OTHER 10 uM; ACTIVE | Not recorded | pubchem.aid:2546
|
| Viability Counterscreen for Primary qHTS for Inhibitors of ATXN expression | OTHER 7.0795 uM; NOT_DETERMINED | Not recorded | PMID:35787375
|
| Viability Counterscreen for Primary qHTS for Inhibitors of ATXN expression | OTHER 7.0795 uM; NOT_DETERMINED | UniProtKB:Q99700 | PMID:35787375
|
| qHTS Assay for Inhibitors of Hepatitis C Virus (HCV) | OTHER 3.5481 uM; ACTIVE | Not recorded | pubchem.aid:651820
|
| qHTS Assay for Inhibitors of Influenza NS1 Protein Function | OTHER 10 uM; INACTIVE | Not recorded | pubchem.aid:2326
|
| qHTS Assay for Lipid Storage Modulators in Drosophila S3 Cells | OTHER 16.3601 uM; NOT_DETERMINED | Not recorded | pubchem.aid:2685
|
| qHTS Assay for Modulators of miRNAs and/or Inhibitors of miR-21 | OTHER 26.1216 uM; NOT_DETERMINED | Not recorded | pubchem.aid:2289
|
| qHTS Assay for Modulators of miRNAs and/orActivators of miR-21 | OTHER 26.1216 uM; NOT_DETERMINED | Not recorded | pubchem.aid:2288
|
| qHTS Assay for Small Molecule Inhibitors of Mitochondrial Division or Activators of Mitochondrial Fusion | OTHER 11.2202 uM; INACTIVE | Not recorded | pubchem.aid:485298
|
| qHTS Assay to Find Inhibitors of Chronic Active B-Cell Receptor Signaling | OTHER 35.4813 uM; NOT_DETERMINED | Not recorded | pubchem.aid:493014
|
| qHTS Assay to Find Inhibitors of Pin1 | OTHER 25.1189 uM; NOT_DETERMINED | Not recorded | pubchem.aid:504891
|
| qHTS for Antagonists of gsp, the Etiologic Mutation Responsible for Fibrous Dysplasia/McCune-Albright Syndrome: qHTS | OTHER 35.4813 uM; NOT_DETERMINED | UniProtKB:P63092 | pubchem.aid:624288
|
| qHTS for Inhibitors of TGF-b | OTHER 22.3872 uM; NOT_DETERMINED | Not recorded | pubchem.aid:588855
|
| qHTS for Inhibitors of TGF-b: Cytotox Counterscreen | OTHER 3.9811 uM; INACTIVE | Not recorded | pubchem.aid:588856
|
| qHTS for Inhibitors of human tyrosyl-DNA phosphodiesterase 1 (TDP1): qHTS in cells in absence of CPT | OTHER 25.929 uM; NOT_DETERMINED | Not recorded | pubchem.aid:686978
|
| qHTS for Inhibitors of human tyrosyl-DNA phosphodiesterase 1 (TDP1): qHTS in cells in presence of CPT | OTHER 20.5962 uM; ACTIVE | Not recorded | pubchem.aid:686979
|
Elsewhere in the fleet
- AntibioticMech — CHEBI:78688 (same structure, same inchikey)
Explore across X-Mech
AntibioticMech (2,4-diacetylphloroglucinol) ↗
MediaIngredientMech (CHEBI:78688) ↗
TaxonMech: Pseudomonas fluorescens (NCBITaxon:294) ↗
TaxonMech: Pseudomonas fluorescens (NCBITaxon:294) ↗
PathwayMech (mibig:BGC0000280) ↗
PathwayMech (mibig:BGC0000281) ↗
Open questions 1
shared-structure — This structure is reported by more than one MIBiG entry: BGC0000280, BGC0000281. Confirm they describe the same compound rather than an upstream cross-reference error.
Provenance
| Source concept | Source | Version |
|---|---|---|
| BGC0000280 | MIBIG | 5 |
| BGC0000281 | MIBIG | 3 |
| CHEBI:78688 | CHEBI | 3-star |
This page is generated from data/natural_products/polyketides/2-4-diacetylphloroglucinol.yaml, which is generated from the committed inventories. Neither is edited by hand.