monactin

naturalproductmech:mibig-02e3a91d63 MINTED SEEDED cytotoxicenzyme inhibitor

Grounding review: No 3-star ChEBI entry shares this structure, so the record keeps a content-hashed identifier.

What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

Structure

Standard InChIKey	YPUPRVWRYDPGCW-IDBRQATOSA-N
SMILES	CC[C@@H]1C[C@H]2CC[C@H](O2)[C@@H](C(=O)O[C@H](C[C@@H]3CC[C@@H](O3)[C@H](C(=O)O[C@@H](C[C@H]4CC[C@H](O4)[C@@H](C(=O)O[C@H](C[C@@H]5CC[C@@H](O5)[C@H](C(=O)O1)C)C)C)C)C)C)C
Stereochemistry	fully defined
Cross-references	pubchem:15560592

Filing pathway

Polyketides — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is pks, from MIBiG.

Producer organisms 2

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

Taxon	Basis	Cluster	Evidence
Streptomyces griseus subsp. griseus NCBITaxon:67263	SOURCE_ASSERTION	mibig:BGC0000243	PMID:10858335
Streptomyces griseus subsp. griseus NCBITaxon:67263	SOURCE_ASSERTION	mibig:BGC0000244	PMID:10858335

Occurrences 0

None cited.

Biosynthetic gene clusters 2

Accession	Host	Locus evidence	Genome
mibig:BGC0000243	Streptomyces griseus subsp. griseus NCBITaxon:67263	CLUSTER_UNSTATED	genbank:AF263011.1
mibig:BGC0000244	Streptomyces griseus subsp. griseus NCBITaxon:67263	CLUSTER_UNSTATED	genbank:AF263012.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Mechanism & Biosynthesis Overview

Bioactivities 25

Entries preserve source qualifiers, targets and evidence. Repeated assay names or values are not independent replications; inspect source detail for differences.

Explore across X-Mech

AntibioticMech (monactin) ↗ MediaIngredientMech (naturalproductmech:mibig-02e3a91d63) ↗ TaxonMech: Streptomyces griseus subsp. griseus (NCBITaxon:67263) ↗ TaxonMech: Streptomyces griseus subsp. griseus (NCBITaxon:67263) ↗ PathwayMech (mibig:BGC0000243) ↗ PathwayMech (mibig:BGC0000244) ↗

Open questions 1

shared-structure — This structure is reported by more than one MIBiG entry: BGC0000243, BGC0000244. Confirm they describe the same compound rather than an upstream cross-reference error.

Provenance

Source concept	Source	Version
BGC0000243	MIBIG	4
BGC0000244	MIBIG	4

This page is generated from data/natural_products/polyketides/monactin.yaml, which is generated from the committed inventories. Neither is edited by hand.

Assay	Result	Target enzyme	Source detail
GF-AFC viability counterscreen for inhibitors of alpha-synuclein gene (SNCA) expression	OTHER 0.0998 uM; NOT_DETERMINED	Not recorded	pubchem.aid:1671193 assay GF-AFC viability counterscreen for inhibitors of alpha-synuclein gene (SNCA) expression evidence reference pubchem.aid:1671193 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1671193, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 0.0998 units uM
HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_CherryPick_Activity_Set3	OTHER 0.506 uM; ACTIVE	Not recorded	pubchem.aid:720589 assay HepG2 Cytotoxicity Assay Measured in Cell-Based System Using Plate Reader - 7071-02_Inhibitor_Dose_CherryPick_Activity_Set3 evidence reference pubchem.aid:720589 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 720589, deposited by Broad Institute; assay type Confirmatory. Source endpoint: AbsAC35_uM. call ACTIVE measurement type OTHER value 0.506 units uM activity class CYTOTOXIC
Luminescence Cell-Based Primary HTS to identify inhibitors of the oncoprotein EWS/Fli transcriptional activity Measured in Cell-Based System Using Plate Reader - 7014-01_Inhibitor_Dose_CherryPick_Activity	OTHER 0.0992 uM; ACTIVE	Not recorded	pubchem.aid:686920 assay Luminescence Cell-Based Primary HTS to identify inhibitors of the oncoprotein EWS/Fli transcriptional activity Measured in Cell-Based System Using Plate Reader - 7014-01_Inhibitor_Dose_CherryPick_Activity evidence reference pubchem.aid:686920 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 686920, deposited by Broad Institute; assay type Confirmatory. Source endpoint: AbsAC35_uM. call ACTIVE measurement type OTHER value 0.0992 units uM activity class ENZYME_INHIBITOR
Luminescence cell-based Retest at Dose assay to determine EWS/Fli1 dependent A673 mammalian cell cytotoxity Measured in Cell-Based System Using Plate Reader - 7014-03_Inhibitor_Dose_CherryPick_Activity	OTHER 0.243 uM; ACTIVE	Not recorded	pubchem.aid:720587 assay Luminescence cell-based Retest at Dose assay to determine EWS/Fli1 dependent A673 mammalian cell cytotoxity Measured in Cell-Based System Using Plate Reader - 7014-03_Inhibitor_Dose_CherryPick_Activity evidence reference pubchem.aid:720587 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 720587, deposited by Broad Institute; assay type Confirmatory. Source endpoint: AbsAC35_uM. call ACTIVE measurement type OTHER value 0.243 units uM activity class CYTOTOXIC
Luminescence cell-based Retest at Dose assay to determine EWS/Fli1 dependent TC71 mammalian cell cytotoxity Measured in Cell-Based System Using Plate Reader - 7014-04_Inhibitor_Dose_CherryPick_Activity	OTHER 26 uM; ACTIVE	Not recorded	pubchem.aid:720570 assay Luminescence cell-based Retest at Dose assay to determine EWS/Fli1 dependent TC71 mammalian cell cytotoxity Measured in Cell-Based System Using Plate Reader - 7014-04_Inhibitor_Dose_CherryPick_Activity evidence reference pubchem.aid:720570 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 720570, deposited by Broad Institute; assay type Confirmatory. Source endpoint: Max_Activity_Conc_uM. call ACTIVE measurement type OTHER value 26.0 units uM activity class CYTOTOXIC
P-glycoprotein substrates identified in KB-8-5-11 adenocarcinoma cell line, qHTS therapeutic library screen	OTHER 1.2182 uM; ACTIVE	Not recorded	PMID:31515284 assay P-glycoprotein substrates identified in KB-8-5-11 adenocarcinoma cell line, qHTS therapeutic library screen evidence reference PMID:31515284 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1346987, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call ACTIVE measurement type OTHER value 1.2182 units uM
Primary fluorescence-based thiol-reactive (MSTI) qHTS assay for identification of artifact compounds.	OTHER 5.0043 uM; NOT_DETERMINED	Not recorded	pubchem.aid:1845221 assay Primary fluorescence-based thiol-reactive (MSTI) qHTS assay for identification of artifact compounds. evidence reference pubchem.aid:1845221 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1845221, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 5.0043 units uM
Primary qHTS assay for inhibitors of Glutamate pyruvate transaminase 2 (GPT2)	OTHER 2.8184 uM; NOT_DETERMINED	Not recorded	pubchem.aid:1671188 assay Primary qHTS assay for inhibitors of Glutamate pyruvate transaminase 2 (GPT2) evidence reference pubchem.aid:1671188 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1671188, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 2.8184 units uM
Primary qHTS assay for inhibitors of Glutamate pyruvate transaminase 2 (GPT2)	OTHER 2.8184 uM; NOT_DETERMINED	UniProtKB:Q8TD30	pubchem.aid:1671188 assay Primary qHTS assay for inhibitors of Glutamate pyruvate transaminase 2 (GPT2) evidence reference pubchem.aid:1671188 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1671188, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 2.8184 units uM target enzyme UniProtKB:Q8TD30
Primary qHTS for inhibitors of NSP2Pro chikungunya virus (CHIKV)	OTHER 0.2329 uM; NOT_DETERMINED	UniProtKB:Q8JUX6	pubchem.aid:1964107 assay Primary qHTS for inhibitors of NSP2Pro chikungunya virus (CHIKV) evidence reference pubchem.aid:1964107 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1964107, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 0.2329 units uM target enzyme UniProtKB:Q8JUX6
Primary qHTS for inhibitors of nuclear receptor binding SET domain protein 2 (NSD2) in an RCH-ACV wild type WT cells (2C)	OTHER 0.0242 uM; ACTIVE	Not recorded	pubchem.aid:1645877 assay Primary qHTS for inhibitors of nuclear receptor binding SET domain protein 2 (NSD2) in an RCH-ACV wild type WT cells (2C) evidence reference pubchem.aid:1645877 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1645877, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call ACTIVE measurement type OTHER value 0.0242 units uM activity class ENZYME_INHIBITOR
Primary qHTS for inhibitors of nuclear receptor binding SET domain protein 2 (NSD2) in an RCH-ACV wild type WT cells (2C)	OTHER 0.0242 uM; ACTIVE	UniProtKB:O96028	pubchem.aid:1645877 assay Primary qHTS for inhibitors of nuclear receptor binding SET domain protein 2 (NSD2) in an RCH-ACV wild type WT cells (2C) evidence reference pubchem.aid:1645877 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1645877, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call ACTIVE measurement type OTHER value 0.0242 units uM target enzyme UniProtKB:O96028 activity class ENZYME_INHIBITOR
Primary qHTS for inhibitors of nuclear receptor binding SET domain protein 2 (NSD2) in an isogenic RCH-ACV NSD2 p.E1099K mutant (9B)	OTHER 0.0216 uM; ACTIVE	Not recorded	pubchem.aid:1645876 assay Primary qHTS for inhibitors of nuclear receptor binding SET domain protein 2 (NSD2) in an isogenic RCH-ACV NSD2 p.E1099K mutant (9B) evidence reference pubchem.aid:1645876 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1645876, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call ACTIVE measurement type OTHER value 0.0216 units uM activity class ENZYME_INHIBITOR
Primary qHTS for inhibitors of nuclear receptor binding SET domain protein 2 (NSD2) in an isogenic RCH-ACV NSD2 p.E1099K mutant (9B)	OTHER 0.0216 uM; ACTIVE	UniProtKB:O96028	pubchem.aid:1645876 assay Primary qHTS for inhibitors of nuclear receptor binding SET domain protein 2 (NSD2) in an isogenic RCH-ACV NSD2 p.E1099K mutant (9B) evidence reference pubchem.aid:1645876 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1645876, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call ACTIVE measurement type OTHER value 0.0216 units uM target enzyme UniProtKB:O96028 activity class ENZYME_INHIBITOR
Primary qHTS to identify anti-liver cancer compounds using libraries of approved drugs and bioactive compounds	OTHER 0.2818 uM; NOT_DETERMINED	Not recorded	pubchem.aid:2202548 assay Primary qHTS to identify anti-liver cancer compounds using libraries of approved drugs and bioactive compounds evidence reference pubchem.aid:2202548 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 2202548, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 0.2818 units uM
Viability qHTS for spleen associated tyrosine kinase inhibitor (SYKi) drug resistant MV4-11 cells	OTHER 0.0385 uM; ACTIVE	Not recorded	pubchem.aid:1963824 assay Viability qHTS for spleen associated tyrosine kinase inhibitor (SYKi) drug resistant MV4-11 cells evidence reference pubchem.aid:1963824 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1963824, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call ACTIVE measurement type OTHER value 0.0385 units uM
Viability qHTS for spleen associated tyrosine kinase inhibitor (SYKi) drug resistant MV4-11 cells	OTHER 0.0385 uM; ACTIVE	UniProtKB:P43405	pubchem.aid:1963824 assay Viability qHTS for spleen associated tyrosine kinase inhibitor (SYKi) drug resistant MV4-11 cells evidence reference pubchem.aid:1963824 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1963824, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call ACTIVE measurement type OTHER value 0.0385 units uM target enzyme UniProtKB:P43405
Viability qHTS for spleen associated tyrosine kinase inhibitor (SYKi) naive MV4-11 cells	OTHER 0.0343 uM; ACTIVE	Not recorded	pubchem.aid:1963823 assay Viability qHTS for spleen associated tyrosine kinase inhibitor (SYKi) naive MV4-11 cells evidence reference pubchem.aid:1963823 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1963823, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call ACTIVE measurement type OTHER value 0.0343 units uM
Viability qHTS for spleen associated tyrosine kinase inhibitor (SYKi) naive MV4-11 cells	OTHER 0.0343 uM; ACTIVE	UniProtKB:P43405	pubchem.aid:1963823 assay Viability qHTS for spleen associated tyrosine kinase inhibitor (SYKi) naive MV4-11 cells evidence reference pubchem.aid:1963823 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1963823, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call ACTIVE measurement type OTHER value 0.0343 units uM target enzyme UniProtKB:P43405
Counterscreen for antagonists of COUP-TFII (NR2F2): Luminescence-based cell-based high throughput assay to identify inhibitors of the Herpes Virus Virion Protein 16 (VP16)	ACTIVE	Not recorded	pubchem.aid:687007 assay Counterscreen for antagonists of COUP-TFII (NR2F2): Luminescence-based cell-based high throughput assay to identify inhibitors of the Herpes Virus Virion Protein 16 (VP16) evidence reference pubchem.aid:687007 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 687007, deposited by The Scripps Research Institute Molecular Screening Center; assay type Screening. Target accession NP_044650 was left ungrounded because PubChem did not provide a UniProtKB accession. call ACTIVE activity class ENZYME_INHIBITOR
Counterscreen for inhibitors of cell surface Prion Protein (PrPc): Luminescence-based cell-based high throughput assay to identify compounds that are cytotoxic to LD9 cells	ACTIVE	Not recorded	pubchem.aid:743201 assay Counterscreen for inhibitors of cell surface Prion Protein (PrPc): Luminescence-based cell-based high throughput assay to identify compounds that are cytotoxic to LD9 cells evidence reference pubchem.aid:743201 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 743201, deposited by The Scripps Research Institute Molecular Screening Center; assay type Screening. Target accession NP_000302 was left ungrounded because PubChem did not provide a UniProtKB accession. call ACTIVE activity class CYTOTOXIC
Cytotoxic Profiling of Annotated Libraries Using Quantitative High-Throughput Screening	ACTIVE	Not recorded	PMID:31498718 assay Cytotoxic Profiling of Annotated Libraries Using Quantitative High-Throughput Screening evidence reference PMID:31498718 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1296008, deposited by NCGC; assay type Confirmatory. call ACTIVE activity class CYTOTOXIC
Luminescence Cell-Based Primary HTS to identify inhibitors of the oncoprotein EWS/Fli transcriptional activity Measured in Cell-Based System Using Plate Reader - 7014-01_Inhibitor_SinglePoint_HTS_Activity	ACTIVE	Not recorded	pubchem.aid:651661 assay Luminescence Cell-Based Primary HTS to identify inhibitors of the oncoprotein EWS/Fli transcriptional activity Measured in Cell-Based System Using Plate Reader - 7014-01_Inhibitor_SinglePoint_HTS_Activity evidence reference pubchem.aid:651661 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 651661, deposited by Broad Institute; assay type Screening. call ACTIVE activity class ENZYME_INHIBITOR
Luminescence-based cell-based high throughput confirmation assay to identify inhibitors of COUP-TFII (NR2F2)	ACTIVE	Not recorded	pubchem.aid:687008 assay Luminescence-based cell-based high throughput confirmation assay to identify inhibitors of COUP-TFII (NR2F2) evidence reference pubchem.aid:687008 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 687008, deposited by The Scripps Research Institute Molecular Screening Center; assay type Screening. Target accession NP_066285 was left ungrounded because PubChem did not provide a UniProtKB accession. call ACTIVE activity class ENZYME_INHIBITOR
Luminescence-based cell-based primary high throughput screening assay to identify inhibitors of COUP-TFII (NR2F2)	ACTIVE	Not recorded	pubchem.aid:686940 assay Luminescence-based cell-based primary high throughput screening assay to identify inhibitors of COUP-TFII (NR2F2) evidence reference pubchem.aid:686940 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 686940, deposited by The Scripps Research Institute Molecular Screening Center; assay type Screening. Target accession NP_066285 was left ungrounded because PubChem did not provide a UniProtKB accession. call ACTIVE activity class ENZYME_INHIBITOR notes 10 further PubChem assay results are in data/raw/pubchem_bioassay.tsv but not written here.