antimycin A

CHEBI:2762 EXACT SEEDED antifungalcytotoxic

What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

A nine-membered bis-lactone having methyl substituents at the 2- and 6-positions, an n-hexyl substituent at the 8-position, an acyloxy substituent at the 7-position and an aroylamido substituent at the 3-position. It is produced by Streptomyces bacteria and has found commercial use as a fish poison.

Structure

Standard InChIKey	UIFFUZWRFRDZJC-SBOOETFBSA-N
SMILES	CCCCCC[C@@H]1[C@H]([C@@H](OC(=O)[C@H]([C@H](OC1=O)C)NC(=O)C2=C(C(=CC=C2)NC=O)O)C)OC(=O)CC(C)C
Stereochemistry	fully defined
Cross-references	pubchem:14957

Filing pathway

Unclassified — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is nrps, pks, from MIBiG.

Producer organisms 1

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

Taxon	Basis	Cluster	Evidence
Streptomyces sp. S4 NCBITaxon:889487	BGC_CHARACTERIZED	mibig:BGC0000958	PMID:21857911

Occurrences 7

Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.

Taxon	Source	Reference
Streptomyces wadayamensis NCBITaxon:141454	LOTUS	DOI:10.1128/GENOMEA.00625-14
Streptomyces NCBITaxon:1883	LOTUS	DOI:10.1021/NP0001676
Streptomyces NCBITaxon:1883	LOTUS	DOI:10.1371/JOURNAL.PONE.0022028
Streptomyces antibioticus NCBITaxon:1890	LOTUS	DOI:10.3390/MOLECULES22040557
Streptomyces setonii NCBITaxon:1911	LOTUS	DOI:10.1007/BF01027810
Streptomyces argillaceus NCBITaxon:41951	LOTUS	DOI:10.1371/JOURNAL.PONE.0198145
Streptomyces baarnensis NCBITaxon:66872	LOTUS	DOI:10.1007/BF01027810

Biosynthetic gene clusters 1

Accession	Host	Locus evidence	Genome
mibig:BGC0000958	Streptomyces sp. S4 NCBITaxon:889487	CLUSTER_DEMONSTRATED	genbank:NZ_FR873698.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Mechanism & Biosynthesis Overview

Bioactivities 25

Entries preserve source qualifiers, targets and evidence. Repeated assay names or values are not independent replications; inspect source detail for differences.

Elsewhere in the fleet

AntibioticMech — CHEBI:2762 (same structure, same inchikey)

Explore across X-Mech

AntibioticMech (antimycin A) ↗ MediaIngredientMech (CHEBI:2762) ↗ TaxonMech: Streptomyces sp. S4 (NCBITaxon:889487) ↗ PathwayMech (mibig:BGC0000958) ↗

Provenance

Source concept	Source	Version
BGC0000958	MIBIG	5
CHEBI:2762	CHEBI	3-star

This page is generated from data/natural_products/unclassified/antimycin-a.yaml, which is generated from the committed inventories. Neither is edited by hand.

Assay	Result	Target enzyme	Source detail
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: AML-191 cell line	OTHER 0.2633 uM; ACTIVE	Not recorded	pubchem.aid:2202236 assay BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: AML-191 cell line evidence reference pubchem.aid:2202236 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 2202236, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call ACTIVE measurement type OTHER value 0.2633 units uM
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: HNT-34 cell line	OTHER 7.4219 uM; NOT_DETERMINED	Not recorded	pubchem.aid:2202234 assay BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: HNT-34 cell line evidence reference pubchem.aid:2202234 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 2202234, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 7.4219 units uM
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: MV-4-11 cell line	OTHER 1.3198 uM; ACTIVE	Not recorded	pubchem.aid:2202233 assay BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: MV-4-11 cell line evidence reference pubchem.aid:2202233 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 2202233, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call ACTIVE measurement type OTHER value 1.3198 units uM
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: OCI-AML3 cell line	OTHER 0.132 uM; NOT_DETERMINED	Not recorded	pubchem.aid:2202232 assay BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: OCI-AML3 cell line evidence reference pubchem.aid:2202232 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 2202232, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 0.132 units uM
BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: SET-2 cell line	OTHER 0.0934 uM; NOT_DETERMINED	Not recorded	pubchem.aid:2202231 assay BET inhibitor-based combinations targeting novel dependencies in MECOM-rearranged (r) AML: SET-2 cell line evidence reference pubchem.aid:2202231 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 2202231, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 0.0934 units uM
Cellular viability qHTS for adrenocortical cancer (ACC) cell line NCI-H295R	OTHER 1.9953 uM; NOT_DETERMINED	Not recorded	pubchem.aid:1963990 assay Cellular viability qHTS for adrenocortical cancer (ACC) cell line NCI-H295R evidence reference pubchem.aid:1963990 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1963990, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 1.9953 units uM
Cellular viability qHTS for adrenocortical cancer (ACC) cell line SW-13	OTHER 0.7943 uM; NOT_DETERMINED	Not recorded	pubchem.aid:1963989 assay Cellular viability qHTS for adrenocortical cancer (ACC) cell line SW-13 evidence reference pubchem.aid:1963989 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1963989, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 0.7943 units uM
Cellular viability qHTS for anaplastic thyroid cancer (ATC) cell line 8505C	OTHER 0.5506 uM; NOT_DETERMINED	Not recorded	pubchem.aid:2202553 assay Cellular viability qHTS for anaplastic thyroid cancer (ATC) cell line 8505C evidence reference pubchem.aid:2202553 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 2202553, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 0.5506 units uM
Cellular viability qHTS for anaplastic thyroid cancer (ATC) cell line THJ-11T	OTHER 0.0778 uM; NOT_DETERMINED	Not recorded	pubchem.aid:2202552 assay Cellular viability qHTS for anaplastic thyroid cancer (ATC) cell line THJ-11T evidence reference pubchem.aid:2202552 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 2202552, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 0.0778 units uM
Cellular viability qHTS for anaplastic thyroid cancer (ATC) cell line THJ-16T	OTHER 0.3474 uM; ACTIVE	Not recorded	pubchem.aid:2202551 assay Cellular viability qHTS for anaplastic thyroid cancer (ATC) cell line THJ-16T evidence reference pubchem.aid:2202551 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 2202551, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call ACTIVE measurement type OTHER value 0.3474 units uM activity class CYTOTOXIC
Counterscreen qHTS for Inhibitors of Tau Fibril Formation, Fluorescence Polarization	OTHER 12.5893 uM; NOT_DETERMINED	Not recorded	pubchem.aid:1463 assay Counterscreen qHTS for Inhibitors of Tau Fibril Formation, Fluorescence Polarization evidence reference pubchem.aid:1463 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1463, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 12.5893 units uM
High-throughput drug toxicity screening in the BPDCN cell line Cal-1	OTHER 0.0935 uM; INACTIVE	Not recorded	pubchem.aid:1224825 assay High-throughput drug toxicity screening in the BPDCN cell line Cal-1 evidence reference pubchem.aid:1224825 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1224825, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call INACTIVE measurement type OTHER value 0.0935 units uM
High-throughput drug toxicity screening in the BPDCN cell line Gen2.2	OTHER 0.0132 uM; INACTIVE	Not recorded	pubchem.aid:1224824 assay High-throughput drug toxicity screening in the BPDCN cell line Gen2.2 evidence reference pubchem.aid:1224824 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1224824, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call INACTIVE measurement type OTHER value 0.0132 units uM
NCATS anti-infectives library activity on the primary C. elegans qHTS viability assay	OTHER 7.2747 uM; ACTIVE	Not recorded	PMID:36786055 assay NCATS anti-infectives library activity on the primary C. elegans qHTS viability assay evidence reference PMID:36786055 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1745855, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call ACTIVE measurement type OTHER value 7.2747 units uM
NS1 protein of Zika virus - qHTS assay to identify small molecule inhibitors in human HEK 293 cells	OTHER 2.9566 uM; ACTIVE	Not recorded	pubchem.aid:1347053 assay NS1 protein of Zika virus - qHTS assay to identify small molecule inhibitors in human HEK 293 cells evidence reference pubchem.aid:1347053 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1347053, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. Target accession ASK51714 was left ungrounded because PubChem did not provide a UniProtKB accession. call ACTIVE measurement type OTHER value 2.9566 units uM
P-glycoprotein substrates identified in KB-8-5-11 adenocarcinoma cell line, qHTS therapeutic library screen	OTHER 0.4176 uM; NOT_DETERMINED	Not recorded	PMID:31515284 assay P-glycoprotein substrates identified in KB-8-5-11 adenocarcinoma cell line, qHTS therapeutic library screen evidence reference PMID:31515284 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1346987, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 0.4176 units uM
Primary qHTS Assay for Inhibitors of Recombinant Selenoprotein Glutathione Peroxidase 4 (GPX4)	OTHER 25.4789 uM; NOT_DETERMINED	Not recorded	pubchem.aid:1845192 assay Primary qHTS Assay for Inhibitors of Recombinant Selenoprotein Glutathione Peroxidase 4 (GPX4) evidence reference pubchem.aid:1845192 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1845192, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 25.4789 units uM
Primary qHTS Assay for Inhibitors of Recombinant Selenoprotein Glutathione Peroxidase 4 (GPX4)	OTHER 25.4789 uM; NOT_DETERMINED	UniProtKB:P36969	pubchem.aid:1845192 assay Primary qHTS Assay for Inhibitors of Recombinant Selenoprotein Glutathione Peroxidase 4 (GPX4) evidence reference pubchem.aid:1845192 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1845192, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 25.4789 units uM target enzyme UniProtKB:P36969
Primary qHTS for Identification of Small Molecule Activators of Huntingtin-Antisense Promoter Activity	OTHER 0.1086 uM; INACTIVE	Not recorded	pubchem.aid:1508624 assay Primary qHTS for Identification of Small Molecule Activators of Huntingtin-Antisense Promoter Activity evidence reference pubchem.aid:1508624 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1508624, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call INACTIVE measurement type OTHER value 0.1086 units uM
Primary qHTS for Identification of Small Molecule Activators of Huntingtin-Antisense Promoter Activity	OTHER 0.1086 uM; INACTIVE	UniProtKB:P42858	pubchem.aid:1508624 assay Primary qHTS for Identification of Small Molecule Activators of Huntingtin-Antisense Promoter Activity evidence reference pubchem.aid:1508624 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1508624, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call INACTIVE measurement type OTHER value 0.1086 units uM target enzyme UniProtKB:P42858
Primary qHTS for Identification of Small Molecule Inhibitors of Huntingtin Promoter Activity	OTHER 0.0863 uM; NOT_DETERMINED	Not recorded	pubchem.aid:1508623 assay Primary qHTS for Identification of Small Molecule Inhibitors of Huntingtin Promoter Activity evidence reference pubchem.aid:1508623 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1508623, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 0.0863 units uM
Primary qHTS for Identification of Small Molecule Inhibitors of Huntingtin Promoter Activity	OTHER 0.0863 uM; NOT_DETERMINED	UniProtKB:P42858	pubchem.aid:1508623 assay Primary qHTS for Identification of Small Molecule Inhibitors of Huntingtin Promoter Activity evidence reference pubchem.aid:1508623 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1508623, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 0.0863 units uM target enzyme UniProtKB:P42858
Primary qHTS for Inhibitors of ATXN expression	OTHER 30.6114 uM; NOT_DETERMINED	Not recorded	PMID:35787375 assay Primary qHTS for Inhibitors of ATXN expression evidence reference PMID:35787375 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1745845, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 30.6114 units uM
Primary qHTS for Inhibitors of ATXN expression	OTHER 30.6114 uM; NOT_DETERMINED	UniProtKB:Q99700	PMID:35787375 assay Primary qHTS for Inhibitors of ATXN expression evidence reference PMID:35787375 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1745845, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 30.6114 units uM target enzyme UniProtKB:Q99700
Primary qHTS for Inhibitors of N-terminal methyltransferase 1 (NTMT1): MTaseGlo Assay	OTHER 15.2369 uM; NOT_DETERMINED	Not recorded	pubchem.aid:1645870 assay Primary qHTS for Inhibitors of N-terminal methyltransferase 1 (NTMT1): MTaseGlo Assay evidence reference pubchem.aid:1645870 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1645870, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 15.2369 units uM notes 147 further PubChem assay results are in data/raw/pubchem_bioassay.tsv but not written here.