actinomycin D

CHEBI:27666 ·resolve ·ANTIBACTERIAL ·EXACT SEEDED

Dactinomycin, also known as Actinomycin D, is a cyclic peptide antibiotic that interacts with DNA to inhibit RNA synthesis. — PMID:2410919

Machine-generated and unreviewed. Identity, structure and cross-references come straight from ChEBI's and CARD's own data; no curator has signed off on this record yet.

Classification

nonribosomal peptide antibioticsARO:3009657

Strictly broader compounds or drug classes this molecule belongs to.

CHEBI:15369 CHEBI:15369
ARO:3009657 ARO:3009657

Chemical structure

Computed structure properties
Molecular formula	C62H86N12O16
Charge	0
Average mass	1255.438 Da
Monoisotopic mass	1254.62847 Da
Source	ChEBI
InChIKey	RJURFGZVJUQBHK-IIXSONLDSA-N

SMILES

[H][C@@]12CCCN1C(=O)[C@@H](C(C)C)NC(=O)[C@@H](NC(=O)c1c3nc4c(C(=O)N[C@@H]5C(=O)N[C@H](C(C)C)C(=O)N6CCC[C@@]6([H])C(=O)N(C)CC(=O)N(C)[C@@H](C(C)C)C(=O)O[C@@H]5C)ccc(C)c4oc-3c(C)c(=O)c1N)[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C2=O

InChI

InChI=1S/C62H86N12O16/c1-27(2)42-59(84)73-23-17-19-36(73)57(82)69(13)25-38(75)71(15)48(29(5)6)61(86)88-33(11)44(55(80)65-42)67-53(78)35-22-21-31(9)51-46(35)64-47-40(41(63)50(77)32(10)52(47)90-51)54(79)68-45-34(12)89-62(87)49(30(7)8)72(16)39(76)26-70(14)58(83)37-20-18-24-74(37)60(85)43(28(3)4)66-56(45)81/h21-22,27-30,33-34,36-37,42-45,48-49H,17-20,23-26,63H2,1-16H3,(H,65,80)(H,66,81)(H,67,78)(H,68,79)/t33-,34-,36+,37+,42-,43-,44+,45+,48+,49+/m1/s1

Also called

2-amino-4,6-dimethyl-3-oxo-1-N,9-N-bis-[(18aS)-10c,14,17-trimethyl-5,8,12,15,18-pentaoxo-6c,13t-di(propan-2-yl)-18ar-hexadecahydro-1H-pyrrolo[2,1-i][1,4,7,10,13]oxatetraazacyclohexadecin-9c-yl]-3H-phenoxazine-1,9-dicarboxamide (EXACT_SYNONYM)— chebi
2-amino-N,N'-bis(hexadecahydro-2,5,9-trimethyl-6,13-bis(1-methylethyl)-1,4,7,11,14-pentaoxo-1H-pyrrolo(2,1-i)(1,4,7,10,13)oxatetra-azacyclohexadecin-10-yl)-4,6-dimethyl-3-oxo-3H-phenoxazine-1,9-dicarboxamide (EXACT_SYNONYM)— chebi
ActD (EXACT_SYNONYM)— chebi
Actinomycin D (EXACT_SYNONYM)— chebi
Dactinomycin (EXACT_SYNONYM)— chebi
actinomycin C1 (EXACT_SYNONYM)— chebi
actinomycin IV (EXACT_SYNONYM)— chebi

Cross-references

Equivalent identifiers for this same structure in other resources.

ARO:3000696
beilstein:4173766
cas:50-76-0
drugbank:DB00970
drugcentral:774
kegg.compound:C06770
kegg.drug:D00214
lincs.smallmolecule:LSM-5783
wikipedia.en:Dactinomycin
chembl:CHEMBL1554
pubchem.compound:457193

Activity roles

Every antimicrobial role a source asserts for this compound — the unreduced evidence behind its antimicrobial_class.

CHEBI:33281 CHEBI:33281

Source concepts

Every upstream concept that resolved to this record. The merge is the product: this is what shows ChEBI and CARD are describing the same structure.

Upstream concepts merged into this record
Source	Native ID	Label	Minted CURIE	Version
ARO	`ARO:3000696`	dactinomycin	`antibioticmech:aro-54f32ec9d8`	2026-08-30
CHEBI	`CHEBI:27666`	actinomycin D	`antibioticmech:chebi-2f20e696b0`	2026-08-30

Molecular targets

The molecular entity or process this compound acts on. Each target carries evidence — this is a mechanistic claim, not a classification.

transcription complex DNA NUCLEIC_ACID ATTACKED_COMPONENT

ARO:3000741

CARD/ARO database assertion; target organism, strain, and assay are not specified. ARO describes exposed DNA bases in the transcription complex. Evidence status: DATABASE_ASSERTION_ONLY. Source: CARD_ARO 2026-08-30 (retrieved 2026-08-30).

ARO:3000741 (CARD/ARO asserts targeted_by_antibiotic ARO:3000696 (dactinomycin); database assertion, not a primary citation.)

Mechanism summary

Clinical status: APPROVED

Curation history

SEEDED_FROM_SOURCES 2026-08-30 · seed_from_sources
Seeded from data/raw/ inventories (ARO, CHEBI)
RESEEDED_FROM_SOURCES 2026-08-30 · seed_from_sources
Re-seeded from updated data/raw/ inventories
RESEEDED_FROM_SOURCES 2026-08-30 · seed_from_sources
Re-seeded from updated data/raw/ inventories
RESEEDED_FROM_SOURCES 2026-08-31 · seed_from_sources
Re-seeded from updated data/raw/ inventories
RESEEDED_FROM_SOURCES 2026-08-31 · seed_from_sources
Re-seeded from updated data/raw/ inventories
RESEEDED_FROM_SOURCES 2026-08-31 · seed_from_sources
Re-seeded from updated data/raw/ inventories

Provenance

Seeded by scripts/seed_from_sources.py from the committed inventories in data/raw/. View the record.