AntibioticMech

isoniazide

CHEBI:6030 ·resolve ·ANTIMYCOBACTERIAL ·EXACT SEEDED

A carbohydrazide obtained by formal condensation between pyridine-4-carboxylic acid and hydrazine. — ChEBI

Classification

isoniazid-like antibioticARO:3007152

Strictly broader compounds or drug classes this molecule belongs to.

• CHEBI:35363 CHEBI:35363
• ARO:3007152 ARO:3007152

Chemical structure

Computed structure properties

Molecular formula	C6H7N3O
Charge	0
Average mass	137.142 Da
Monoisotopic mass	137.05891 Da
Source	ChEBI
InChIKey	QRXWMOHMRWLFEY-UHFFFAOYSA-N

SMILES

NNC(=O)c1ccncc1

InChI

InChI=1S/C6H7N3O/c7-9-6(10)5-1-3-8-4-2-5/h1-4H,7H2,(H,9,10)

Also called

• pyridine-4-carbohydrazide (EXACT_SYNONYM)— chebi
• 4-pyridinecarbohydrazide (EXACT_SYNONYM)— chebi
• Isoniazid (EXACT_SYNONYM)— chebi
• Isonicotinsäurehydrazid (EXACT_SYNONYM)— chebi
• isonicotinic acid hydrazide (EXACT_SYNONYM)— chebi
• isonicotinic hydrazide (EXACT_SYNONYM)— chebi
• isonicotinohydrazide (EXACT_SYNONYM)— chebi
• isonicotinoylhydrazide (EXACT_SYNONYM)— chebi
• pyridine-4-carboxylic acid hydrazide (EXACT_SYNONYM)— chebi
• isoniazid (RELATED_SYNONYM)— chebi

Cross-references

Equivalent identifiers for this same structure in other resources.

• ARO:3000520
• cas:54-85-3
• drugbank:DB00951
• drugcentral:1497
• gmelin:82804
• kegg.compound:C07054
• kegg.drug:D00346
• lincs.smallmolecule:LSM-6682
• metacyc.compound:ISONIAZIDE
• reaxys:119374
• wikipedia.en:Isoniazid
• chembl:CHEMBL64
• pubchem.compound:3767

Activity roles

Every antimicrobial role a source asserts for this compound — the unreduced evidence behind its antimicrobial_class.

• CHEBI:33231 CHEBI:33231

Source concepts

Every upstream concept that resolved to this record. The merge is the product: this is what shows ChEBI and CARD are describing the same structure.

Upstream concepts merged into this record

Source	Native ID	Label	Minted CURIE	Version
ARO	ARO:3000520	isoniazid	antibioticmech:aro-416e3ef585	2026-08-30
CHEBI	CHEBI:6030	isoniazide	antibioticmech:chebi-b63c959909	2026-08-30

Molecular targets

The molecular entity or process this compound acts on. Each target carries evidence — this is a mechanistic claim, not a classification.

mycolic acid synthesis enzyme targeted by antibiotic PROTEIN DIRECT_BINDING_TARGET

ARO:3000716

CARD/ARO database assertion; target organism, strain, and assay are not specified. ARO asserts enzymes in mycolic-acid synthesis as targets without naming a downstream process. Evidence status: PRIMARY_EVIDENCE_NEEDED. Source: CARD_ARO 2026-08-30 (retrieved 2026-08-30).

• ARO:3000716 (CARD/ARO asserts targeted_by_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)

antibiotic sensitive inhA PROTEIN DIRECT_BINDING_TARGET

ARO:3003426

CARD/ARO database assertion; target organism, strain, and assay are not specified. InhA is asserted as the antibiotic-sensitive enzyme. Evidence status: PRIMARY_EVIDENCE_NEEDED. Source: CARD_ARO 2026-08-30 (retrieved 2026-08-30).

• ARO:3003426 (CARD/ARO asserts targeted_by_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)

antibiotic sensitive kasA PROTEIN DIRECT_BINDING_TARGET

ARO:3003478

CARD/ARO database assertion; target organism, strain, and assay are not specified. KasA is asserted as the antibiotic-sensitive enzyme. Evidence status: PRIMARY_EVIDENCE_NEEDED. Source: CARD_ARO 2026-08-30 (retrieved 2026-08-30).

• ARO:3003478 (CARD/ARO asserts targeted_by_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)

Mechanism summary

Clinical status

APPROVED

Resistance mechanisms

22 known routes by which microbes resist this compound, grounded in ARO where CARD models them. Grouped by mechanism type — expand a group to see its determinants.

ANTIBIOTIC_TARGET_ALTERATION 20

ANTIBIOTIC_TARGET_ALTERATION

Determinant	ARO ID	Organism	Gene families	Evidence
Mycobacterium tuberculosis Rv1258c mutations confer resistance to pyrazinamide, isoniazid, and streptomycin	ARO:3007186	not organism-specific	—	ARO:3007186 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
Mycobacterium tuberculosis ahpC mutations confer resistance to isoniazid	ARO:3004921	not organism-specific	—	ARO:3004921 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
Mycobacterium tuberculosis ethA mutations conferring resistance to isoniazid	ARO:3005105	not organism-specific	—	ARO:3005105 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
Mycobacterium tuberculosis fabG1 mutations confer resistance to isoniazid	ARO:3004922	not organism-specific	—	ARO:3004922 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
Mycobacterium tuberculosis furA mutations confer resistance to isoniazid	ARO:3004923	not organism-specific	—	ARO:3004923 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
Mycobacterium tuberculosis inbR mutations conferring resistance to isoniazid	ARO:3004924	not organism-specific	—	ARO:3004924 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
Mycobacterium tuberculosis inhA mutations conferring resistance to isoniazid	ARO:3003393	not organism-specific	—	ARO:3003393 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
Mycobacterium tuberculosis iniA mutations conferring resistance to isoniazid	ARO:3007798	not organism-specific	—	ARO:3007798 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
Mycobacterium tuberculosis kasA mutant conferring resistance to isoniazid	ARO:3003463	not organism-specific	—	ARO:3003463 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
Mycobacterium tuberculosis katG mutations conferring resistance to isoniazid	ARO:3003392	not organism-specific	—	ARO:3003392 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
Mycobacterium tuberculosis mmaA3 mutations conferring resistance to isoniazid	ARO:3004929	not organism-specific	—	ARO:3004929 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
Mycobacterium tuberculosis mshA mutations conferring resistance to isoniazid	ARO:3004925	not organism-specific	—	ARO:3004925 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
Mycobacterium tuberculosis mshC mutations conferring resistance to isoniazid	ARO:3004927	not organism-specific	—	ARO:3004927 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
Mycobacterium tuberculosis mymA mutations conferring resistance to isoniazid	ARO:3004928	not organism-specific	—	ARO:3004928 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
Mycobacterium tuberculosis nat mutations conferring resistance to isoniazid	ARO:3004930	not organism-specific	—	ARO:3004930 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
Mycobacterium tuberculosis ndh with mutation conferring resistance to isoniazid	ARO:3003461	not organism-specific	—	ARO:3003461 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
Mycobacterium tuberculosis nudC mutations conferring resistance to isoniazid	ARO:3004931	not organism-specific	—	ARO:3004931 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
Mycobacterium tuberculosis sigI mutations conferring resistance to isoniazid	ARO:3004932	not organism-specific	—	ARO:3004932 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
Mycobacterium tuberculosis variant bovis ndh with mutation conferring resistance to isoniazid	ARO:3003779	not organism-specific	—	ARO:3003779 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
Mycolicibacterium smegmatis ndh with mutation conferring resistance to isoniazid	ARO:3003778	not organism-specific	—	ARO:3003778 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)

ANTIBIOTIC_EFFLUX 2

ANTIBIOTIC_EFFLUX

Determinant	ARO ID	Organism	Gene families	Evidence
efpA	ARO:3003955	not organism-specific	—	ARO:3003955 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)
putative nickel/cobalt transporter	ARO:3007659	not organism-specific	—	ARO:3007659 (CARD/ARO asserts confers_resistance_to_antibiotic ARO:3000520 (isoniazid); database assertion, not a primary citation.)

Curation history

1. SEEDED_FROM_SOURCES 2026-08-30 · seed_from_sources

   Seeded from data/raw/ inventories (ARO, CHEBI)

2. RESEEDED_FROM_SOURCES 2026-08-30 · seed_from_sources

   Re-seeded from updated data/raw/ inventories

3. RESEEDED_FROM_SOURCES 2026-08-30 · seed_from_sources

   Re-seeded from updated data/raw/ inventories

4. RESEEDED_FROM_SOURCES 2026-08-31 · seed_from_sources

   Re-seeded from updated data/raw/ inventories

5. RESEEDED_FROM_SOURCES 2026-08-31 · seed_from_sources

   Re-seeded from updated data/raw/ inventories

6. RESEEDED_FROM_SOURCES 2026-08-31 · seed_from_sources

   Re-seeded from updated data/raw/ inventories

Provenance

Seeded by scripts/seed_from_sources.py from the committed inventories in data/raw/. View the record.