Operational assay

ICP-MS assay of Nd in synthetic-phosphate bioleachate

AssayMech:nd-bioleaching-icp-ms

Bioprocessing

Measure Nd released into filtered laboratory leachate following contact between synthetic Mon2 phosphate feed and biolixiviants produced by G. oxydans B58-derived cultures.

Download source YAML · View source at this revision

Scoped operational assay. Consult the sourced method, controls and limitations; missing protocol details remain explicit research gaps.

Record evidence

Direct genome-scale screening of Gluconobacter oxydans B58 for rare earth element bioleaching — Primary methods describe ICP-MS measurement of filtered leachate after synthetic Nd-phosphate contact with G. oxydans-derived biolixiviants.

Source reference · html:#Sec16 · Source licence: CC-BY-4.0
Direct genome-scale screening of Gluconobacter oxydans B58 for rare earth element bioleaching — The results distinguish Mon2 as the monazite-Nd/rhabdophane-Nd mixture, not pure monazite.

Source reference · html:#Sec4 · Source licence: CC-BY-4.0
Marecos et al. (2025), Supplementary Data 4 — The response column is labelled as micrograms of Nd per gram of NdPO4, not as percent extraction.

Source reference · xlsx:4A!B2 · Source licence: CC-BY-4.0
Marecos et al. (2025), Supplementary Data 4 — The response table reports final pH; the record does not infer a common fixed-pH treatment.

Source reference · xlsx:4A!C2 · Source licence: CC-BY-4.0

Interpretation and limitations

The feed is a synthetic monazite-Nd/rhabdophane-Nd mixture; this method is not demonstrated here for natural ore or industrial operation.
Filtration is reported after leaching; cell removal before mineral contact is unspecified.
Measured solubilization does not establish a purified recovered product, whole-process recovery fraction or scale-transfer result.
The reported mass-normalized response uses the source-labelled NdPO4 denominator; it is not a percentage of total feed Nd.
Reported final pH is an outcome; a common fixed-pH treatment is not established.

Ontology groundings

Assay identity, measured phenotype and tested predicate are distinct relations. A property grounding does not turn that property into an assay identity.

No ontology grounding recorded; see research gaps.

Method summary

After laboratory contact with Mon2, filter leachates at 0.45 micrometres, dilute in 2% trace-metal-grade nitric acid and measure Nd using ICP-MS with a rare-earth calibration mix and Rh internal standard.

Direct genome-scale screening of Gluconobacter oxydans B58 for rare earth element bioleaching — Primary methods describe ICP-MS measurement of filtered leachate after synthetic Nd-phosphate contact with G. oxydans-derived biolixiviants.

Source reference · html:#Sec16 · Source licence: CC-BY-4.0

Measurement and interpretation

Target: Nd in the post-leaching filtrate.
Readout: ICP-MS Nd signal converted using calibration; Supplementary Data 4 reports the response normalized to the source-labelled NdPO4 feed mass.
Unit: ug/g
Interpretation: Micrograms of Nd per gram of NdPO4 as labelled by the study; do not interpret as percent recovery.

Direct genome-scale screening of Gluconobacter oxydans B58 for rare earth element bioleaching — Primary methods describe ICP-MS measurement of filtered leachate after synthetic Nd-phosphate contact with G. oxydans-derived biolixiviants.

Source reference · html:#Sec16 · Source licence: CC-BY-4.0
Marecos et al. (2025), Supplementary Data 4 — The response column is labelled as micrograms of Nd per gram of NdPO4, not as percent extraction.

Source reference · xlsx:4A!B2 · Source licence: CC-BY-4.0

Controls

Wild-type and proxy wild-type cultures, with no-bacteria controls; three biological replicates for selected bacterial strains.

Direct genome-scale screening of Gluconobacter oxydans B58 for rare earth element bioleaching — Primary methods describe ICP-MS measurement of filtered leachate after synthetic Nd-phosphate contact with G. oxydans-derived biolixiviants.

Source reference · html:#Sec16 · Source licence: CC-BY-4.0

Calibration standards, blanks, analytical replicates and Rh internal standard support instrumental quality control.

Direct genome-scale screening of Gluconobacter oxydans B58 for rare earth element bioleaching — Primary methods describe ICP-MS measurement of filtered leachate after synthetic Nd-phosphate contact with G. oxydans-derived biolixiviants.

Source reference · html:#Sec16 · Source licence: CC-BY-4.0

Scoped CMM relationships

Measures process · cmmmech:neodymium

Corpus: cmmmech

The target mechanism explicitly reports ICP-MS of post-leaching filtrates for the Marecos synthetic-phosphate experiment.

Scope: G. oxydans B58-derived cultures and synthetic Mon2 feed; filtered-leachate Nd after laboratory bioleaching.

Target locator: /mechanisms/1

Linked source record: data/records/neodymium.yaml
Source revision: df061de720fb9884dcc6e41369a3049cef52a059

CMMMech neodymium mechanism at df061de720fb9884dcc6e41369a3049cef52a059 — The target mechanism explicitly reports ICP-MS of post-leaching filtrates for the Marecos synthetic-phosphate experiment.

Source reference · /mechanisms/1 · Source licence: CC-BY-4.0
Direct genome-scale screening of Gluconobacter oxydans B58 for rare earth element bioleaching — Primary methods describe ICP-MS measurement of filtered leachate after synthetic Nd-phosphate contact with G. oxydans-derived biolixiviants.

Source reference · html:#Sec16 · Source licence: CC-BY-4.0

Research gaps

metpo: No exact METPO assay identity or solubilization predicate was established for this operational assay; no generic process term is substituted.
protocol_parameters: This record summarizes the cited method; complete culture preparation, sampling and instrument settings remain in the primary protocol.

Curation provenance

Recorded curation events
UTC timestamp	Action and curator	Summary
2026-11-09T02:38:32Z	CREATE · assay-stream-c	Curate initial sourced assay concept or scoped operational assay History source