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Aerobic Denitrification Quorum-Quenching SynCom

Community ID CommunityMech:000115
Ecological State ENGINEERED
Environment laboratory bioreactor — ontology label: laboratory environment
ENVO:01001405 · HabitatMech ↗

A three-member aerobic denitrification SynCom whose quorum-quenching and AHL-mediated interactions improve nitrate removal.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Acinetobacter baumannii NCBITaxon:470 · TaxonMech · TraitMech
CROSS_FEEDER
N/A
  • PMID:38277828 - SUPPORT (IN_VITRO)
    "a synthetic microbial community (SMC) composed of denitrifiers Acinetobacter baumannii N1 (AC), Pseudomonas aeruginosa N2 (PA) and Aeromonas hydrophila (AH) were constructed."
Pseudomonas aeruginosa NCBITaxon:287 · TaxonMech · TraitMech
CROSS_FEEDER
N/A
  • PMID:38277828 - SUPPORT (IN_VITRO)
    "a synthetic microbial community (SMC) composed of denitrifiers Acinetobacter baumannii N1 (AC), Pseudomonas aeruginosa N2 (PA) and Aeromonas hydrophila (AH) were constructed."
Aeromonas hydrophila NCBITaxon:644 · TaxonMech · TraitMech
CROSS_FEEDER
N/A
  • PMID:38277828 - SUPPORT (IN_VITRO)
    "a synthetic microbial community (SMC) composed of denitrifiers Acinetobacter baumannii N1 (AC), Pseudomonas aeruginosa N2 (PA) and Aeromonas hydrophila (AH) were constructed."

Ecological Interactions

The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.

Ecological interaction network for Aerobic Denitrification Quorum-Quenching SynCom Bipartite graph where circles represent taxa and non-circular symbols represent interactions. Rare or untyped interactions share the Other symbol; exact curated types remain in the interaction details.
Taxon
Other

Aeromonas quorum quenching of 3OC12-HSL

PARTIAL - the authors attribute reduced 3OC12-HSL and increased pyocyanin excretion to Aeromonas quorum quenching. The accessible abstract does not describe the perturbation or add-back controls isolating this mediator sequence, or establish mutual fitness benefits.

Participating Taxa: Pseudomonas aeruginosa, Aeromonas hydrophila

Metabolites: N-(3-oxododecanoyl)-L-homoserine lactone (CHEBI:44534 · MediaIngredientMech · CMMMech)

Biological Processes:

Downstream Effects:
→ Pyocyanine-stimulated electron transfer

PARTIAL - the abstract reports that Aeromonas-associated 3OC12-HSL decline stimulates pyocyanin excretion, but does not describe mediator-specific disruption or rescue controls.

Evidence

  • PMID:38277828 - PARTIAL (IN_VITRO)
    "quorum quenching (QQ) by AH, stimulated the excretion of pyocyanin, which could improve the electron transfer from complex III to downstream denitrifying enzymes"

Pyocyanine-stimulated electron transfer

PARTIAL - excreted pyocyanin is proposed to improve electron transfer from complex III to nitrite-reducing enzymes. The abstract does not isolate this contribution from other AHL and energy-metabolism routes, or establish nutritional cross-feeding of pyocyanin.

Participating Taxa: Pseudomonas aeruginosa, Aeromonas hydrophila

Metabolites: pyocyanine (CHEBI:8653 · MediaIngredientMech · CMMMech)

Biological Processes:

Downstream Effects:
→ Efficient nitrate reduction by the three-member SMC

PARTIAL - pyocyanin-associated electron transfer could support the nitrite-reduction component of the community endpoint. The abstract also describes nitrate-reduction and energy-metabolism routes, so this is not a sole explanation of nitrate removal.

Evidence

  • PMID:38277828 - PARTIAL (IN_VITRO)
    "stimulated the excretion of pyocyanin, which could improve the electron transfer from complex III to downstream denitrifying enzymes for NO2--N reduction"

Efficient nitrate reduction by the three-member SMC

The Acinetobacter-Pseudomonas-Aeromonas SMC reached high nitrate reduction efficiency with little nitrite accumulation during aerobic denitrification.

Participating Taxa: Acinetobacter baumannii, Pseudomonas aeruginosa, Aeromonas hydrophila

Metabolites: nitrate (CHEBI:17632 · MediaIngredientMech · CMMMech), nitrite (CHEBI:16301 · MediaIngredientMech · CMMMech)

Biological Processes:

Evidence

  • PMID:38277828 - SUPPORT (IN_VITRO)
    "The nitrate (NO3--N) reduction efficiency of the SMC reached 97 % with little nitrite (NO2--N) accumulation"

Knowledge gaps & discussions (1)

KNOWLEDGE_GAP OPEN Which perturbations isolate quorum quenching and pyocyanin contributions to denitrification?

PMID:38277828 reports community performance and a proposed signal/redox sequence, but the accessible abstract does not report mediator-specific disruption, add-back, or flux controls. It does not establish reciprocal fitness effects, pyocyanin nutritional uptake, or product-removal thermodynamics. Full methods are needed before strengthening either retained edge.

Attaches to: ecological_interactions#Aeromonas quorum quenching of 3OC12-HSL, ecological_interactions#Pyocyanine-stimulated electron transfer, ecological_interactions#Efficient nitrate reduction by the three-member SMC

Environmental Factors

Factor Value Unit
defined synthetic community design Acinetobacter baumannii N1, Pseudomonas aeruginosa N2, and Aeromonas hydrophila were assembled as an SMC. N/A
  • PMID:38277828 - SUPPORT (IN_VITRO)
    "a synthetic microbial community (SMC) composed of denitrifiers Acinetobacter baumannii N1 (AC), Pseudomonas aeruginosa N2 (PA) and Aeromonas hydrophila (AH) were constructed."

Growth Media

nitrate wastewater (aerobic denitrification medium)

Atmosphere AEROBIC

Preparation notes: Synthetic microbial community cultivated under aerobic conditions for aerobic denitrification of nitrate wastewater. Medium composition, temperature, and pH are not reported in the accessible (paywalled) abstract.

Evidence

PMID:38277828

"the application of aerobic denitrification technology in wastewater treatment"

Cross-Mech Suite Links

Explore related entities across the CultureBotAI X-Mech knowledge bases:

HabitatMech: laboratory bioreactor ↗ TaxonMech: Acinetobacter baumannii ↗ TraitMech: Acinetobacter baumannii ↗ TaxonMech: Pseudomonas aeruginosa ↗ TraitMech: Pseudomonas aeruginosa ↗ TaxonMech: Aeromonas hydrophila ↗ TraitMech: Aeromonas hydrophila ↗ CultureMech Growth Media ↗ PathwayMech Pathways ↗