Home ← Back to Communities Text Map Taxonomic UMAP Sequence Map Network Schema All Mech projects

Arabidopsis Phyllosphere SynCom7

Community ID CommunityMech:000093
Ecological State ENGINEERED
Environment phyllosphere — ontology label: plant-associated environment
ENVO:01001001 · HabitatMech ↗

A seven-strain reduced-complexity Arabidopsis phyllosphere SynCom used to test host genetic effects on microbiota composition.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Arabidopsis phyllosphere bacteria — ontology label: Bacteria NCBITaxon:2 · TaxonMech · TraitMech
CROSS_FEEDER
N/A
  • PMID:24743269 - SUPPORT (IN_VITRO)
    "the model plant Arabidopsis thaliana was used in a gnotobiotic system and inoculated with a reduced complexity synthetic bacterial community composed of seven strains representing the most abundant phyla in the phyllosphere."

Ecological Interactions

The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.

Ecological interaction network for Arabidopsis Phyllosphere SynCom7 Bipartite graph where circles represent taxa and non-circular symbols represent interactions. Rare or untyped interactions share the Other symbol; exact curated types remain in the interaction details.
Taxon
Other

Cuticle-Mutant Phyllosphere Abundance and Composition Changes

Cuticle mutants lacs2 and pec1 strongly changed the controlled seven-strain phyllosphere community. Increased total abundance was strongest for lacs2, while responses varied by strain and mutant. Relative ARISA profiles and absolute enumeration are distinct readouts; the proposed nutrient-leakage or cuticle-substrate mechanisms were not directly measured exchange.

Biological Processes:

Evidence

  • PMID:24743269 - SUPPORT (IN_VIVO)
    "Host alleles that resulted in the strongest perturbation of the microbiota relative to the wild-type were lacs2 and pec1"

EIN2 Ethylene-Signaling Composition Shift

Loss of EIN2 reproducibly changed the controlled phyllosphere community composition and Variovorax counts. The total-abundance effect was weak. The genotype experiment implicates ethylene signaling without directly measuring an ethylene concentration or universal bacterial biomass increase.

Metabolites: ethylene (CHEBI:18153 · MediaIngredientMech · CMMMech)

Biological Processes:

Evidence

  • PMID:24743269 - SUPPORT (IN_VIVO)
    "we identified ein2, which is involved in ethylene signaling, as a host factor modulating the community's composition"

Natural-Accession Phyllosphere Community Shift

Four of nine natural Arabidopsis accessions changed composition or abundance of the seven-strain community in the controlled plant assay. Specific causal host genes and transfer of these outcomes to field communities remain unresolved.

Biological Processes:

Evidence

  • PMID:24743269 - SUPPORT (IN_VIVO)
    "of the nine natural accessions tested, four were found to modify community composition and abundance"

Knowledge gaps & discussions (1)

KNOWLEDGE_GAP OPEN Which cuticle or signaling changes cause the strain-specific abundance responses?

PMID:24743269 establishes genotype-associated outcomes, but nutrient leakage, substrate use and specific natural-accession genes remain mechanistic questions. Relative ARISA signals and absolute enumeration must not be interchanged. Construction and inoculation are retained in engineering metadata, not ecological graph nodes.

Attaches to: ecological_interactions#Cuticle-Mutant Phyllosphere Abundance and Composition Changes, ecological_interactions#EIN2 Ethylene-Signaling Composition Shift, ecological_interactions#Natural-Accession Phyllosphere Community Shift

Environmental Factors

Factor Value Unit
defined synthetic community design Seven bacterial strains representing abundant phyllosphere phyla were inoculated onto gnotobiotic Arabidopsis mutants. N/A
  • PMID:24743269 - SUPPORT (IN_VITRO)
    "the model plant Arabidopsis thaliana was used in a gnotobiotic system and inoculated with a reduced complexity synthetic bacterial community composed of seven strains representing the most abundant phyla in the phyllosphere."

Growth Media

nutrient broth (NB) / mineral salt medium (seven-strain phyllosphere SynCom); half-strength MS medium for the gnotobiotic Arabidopsis host

Temperature 28 °C

Preparation notes: Bacterial members grown on nutrient broth (both Methylobacterium strains on mineral salt medium with 0.5% succinate) at 28°C for one to three days, washed and resuspended in 10 mM MgCl2, adjusted to OD600 0.2, mixed 1:1 across the seven strains and diluted to OD600 0.02 for spray inoculation; the Arabidopsis host was cultivated on half-strength MS nutrient medium with vitamins, 0.55% plant agar and 1% sucrose, with seeds stratified for 3 days at 4°C.

Evidence

PMID:24743269

"Strains were grown at 28°C for three days (both Methylobacterium strains), two days (Rhodococcus sp., Variovorax sp., Arthrobacter sp. and S. phyllosphaerae), or one day (Sphingomonas sp. Fr1)."

PMID:24743269

"All other strains were grown on nutrient broth (NB) without additional NaCl"

PMID:24743269

"Plants were grown under short-day conditions (a 9-h photoperiod) in a standard growth chamber"

PMID:24743269

"This solution was then diluted to an OD600 of 0.02."

Cross-Mech Suite Links

Explore related entities across the CultureBotAI X-Mech knowledge bases:

HabitatMech: phyllosphere ↗ TaxonMech: Arabidopsis phyllosphere bacteria ↗ TraitMech: Arabidopsis phyllosphere bacteria ↗ CultureMech Growth Media ↗ PathwayMech Pathways ↗