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Arabidopsis Phyllosphere SynCom7

Community ID CommunityMech:000093
Ecological State ENGINEERED
Environment phyllosphere
ENVO:01001001

A seven-strain reduced-complexity Arabidopsis phyllosphere SynCom used to test host genetic effects on microbiota composition.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Arabidopsis phyllosphere bacteria NCBITaxon:2
CROSS_FEEDER
N/A
  • PMID:24743269 - SUPPORT (IN_VITRO)
    "the model plant Arabidopsis thaliana was used in a gnotobiotic system and inoculated with a reduced complexity synthetic bacterial community composed of seven strains representing the most abundant phyla in the phyllosphere."

Ecological Interactions

Ecological interaction network for Arabidopsis Phyllosphere SynCom7 Bipartite graph where circle nodes represent taxa and each ecological interaction is drawn as a distinct non-circular symbol, with colour repeating the same distinction (niche partitioning).
Taxon
Niche partitioning

Host-Genotype-Structured Assembly

NICHE_PARTITIONING

Evidence

  • PMID:24743269 - SUPPORT (IN_VITRO)
    "the model plant Arabidopsis thaliana was used in a gnotobiotic system and inoculated with a reduced complexity synthetic bacterial community composed of seven strains representing the most abundant phyla in the phyllosphere."

Environmental Factors

Factor Value Unit
defined synthetic community design Seven bacterial strains representing abundant phyllosphere phyla were inoculated onto gnotobiotic Arabidopsis mutants. N/A
  • PMID:24743269 - SUPPORT (IN_VITRO)
    "the model plant Arabidopsis thaliana was used in a gnotobiotic system and inoculated with a reduced complexity synthetic bacterial community composed of seven strains representing the most abundant phyla in the phyllosphere."

Growth Media

nutrient broth (NB) / mineral salt medium (seven-strain phyllosphere SynCom); half-strength MS medium for the gnotobiotic Arabidopsis host

Temperature 28 °C

Preparation notes: Bacterial members grown on nutrient broth (both Methylobacterium strains on mineral salt medium with 0.5% succinate) at 28°C for one to three days, washed and resuspended in 10 mM MgCl2, adjusted to OD600 0.2, mixed 1:1 across the seven strains and diluted to OD600 0.02 for spray inoculation; the Arabidopsis host was cultivated on half-strength MS nutrient medium with vitamins, 0.55% plant agar and 1% sucrose, with seeds stratified for 3 days at 4°C.

Evidence

PMID:24743269

"Strains were grown at 28°C for three days (both Methylobacterium strains), two days (Rhodococcus sp., Variovorax sp., Arthrobacter sp. and S. phyllosphaerae), or one day (Sphingomonas sp. Fr1)."

PMID:24743269

"All other strains were grown on nutrient broth (NB) without additional NaCl"

PMID:24743269

"Plants were grown under short-day conditions (a 9-h photoperiod) in a standard growth chamber, as previously described."

PMID:24743269

"This solution was then diluted to an OD600 of 0.02."