A defined freshwater green-alga and bacterium co-culture pairing axenic Auxenochlorella pyrenoidosa with Pseudomonas_sp1, a phycospheric isolate recovered after high-florfenicol and polylactic acid microplastic stress. Liu et al. isolated Pseudomonas_sp1 from the stressed phycosphere and used the isolate in short co-culture validation experiments showing promoted A. pyrenoidosa growth and Pseudomonas-associated pyridoxal production under florfenicol stress.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Auxenochlorella pyrenoidosa | NCBITaxon:3078 · TaxonMech · TraitMech |
PRIMARY_PRODUCER
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DOMINANT |
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| Pseudomonas sp. Pseudomonas_sp1 — ontology label: Pseudomonas sp. | NCBITaxon:306 · TaxonMech · TraitMech |
CROSS_FEEDER
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N/A |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Pseudomonas Pyridoxal Support Under Florfenicol Stress
CROSS_FEEDINGPARTIAL - Pseudomonas_sp1 promoted A. pyrenoidosa growth during the 13-day florfenicol-stress co-culture, and pyridoxal was detected with the bacterium. Exogenous vitamin B6 also promoted axenic algal growth, supporting pyridoxal as a candidate mediator. These experiments establish association and supplementation sufficiency, not necessity of bacterial pyridoxal or traced transfer.
Source Taxon: Pseudomonas sp. Pseudomonas_sp1
Target Taxon: Auxenochlorella pyrenoidosa
Biological Processes:
- biological process involved in interspecies interaction between organisms (GO:0044419 · PathwayMech)
Evidence
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PMID:41724996 - SUPPORT (IN_VITRO)"co-cultures with Pseudomonas_sp1 promoted microalgal growth within 13"
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PMID:41724996 - PARTIAL (IN_VITRO)"pyridoxal was detected only in the presence of Pseudomonas_sp1"
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PMID:41724996 - PARTIAL (IN_VITRO)"exogenous vitamin B6 alone is sufficient to promote axenic algal growth"
Associated Datasets
| Dataset | Type | Repository | Accession |
|---|---|---|---|
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Pseudomonas_sp1 isolate genome BioProject
Whole-genome sequencing reads for the phycospheric Pseudomonas_sp1 isolate used in the A. pyrenoidosa co-culture validation. |
GENOMICS | NCBI_BIOPROJECT | PRJNA1171391 |
Knowledge gaps & discussions (2)
KNOWLEDGE_GAP OPEN The article body states that Text S12 contains details of the Pseudomonas_sp1 and A. pyrenoidosa co-culture experiment; those supplementary details should be inspected to recover the exact medium, inoculum, volume, and exposure groups for the pairwise assay.
The main article cleanly establishes Pseudomonas_sp1 as the selected partner and reports the 13-day response, but it points cultivation details for that follow-up pairwise assay to Text S12.
Attaches to: growth_media#Pseudomonas_sp1-Auxenochlorella
1 evidence item(s)
Text S12. The co-culture experiment of phycospheric isolates and A. pyrenoidosa
Shows that exact follow-up co-culture details were placed in the supplement rather than the article body.
KNOWLEDGE_GAP OPEN Is bacterial pyridoxal necessary for the algal benefit, and is its transfer directly measurable?
PMID:41724996 supports the defined-pair growth response and vitamin supplementation. Removing pyridoxal production or blocking algal uptake would distinguish mediation from correlated metabolite accumulation. The existing Text S12 medium gap remains; Text S12-S14 were not reviewed. No antibiotic-degradation or reciprocal-fitness claim is added.
Attaches to: ecological_interactions#Pseudomonas Pyridoxal Support Under Florfenicol Stress
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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Primary article - Auxenochlorella-Pseudomonas florfenicol phycosphere coculture
Microbiome article reporting Pseudomonas_sp1 isolation from the florfenicol- and PLA-stressed A. pyrenoidosa phycosphere and validating its growth-promoting effect in co-culture. |
OTHER | doi:10.1186/s40168-026-02335-7 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Florfenicol and PLA microplastic challenge | single florfenicol or florfenicol plus PLA MPs | N/A |
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| Pseudomonas-associated pyridoxal production | N/A | |
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