A defined sixteen-strain drought-tolerant bacterial synthetic community assembled from barley-rhizosphere isolates collected at the Global Change Experimental Facility in Bad Lauchstadt. Rigerte et al. selected isolates with overlapping 16S rDNA sequences to drought- or future-climate-associated amplicon sequence variants, cultured the barley isolate set on YME agar, combined equal proportions of the sixteen single-strain suspensions, and applied the Barley DT-SynCom to barley seedlings before Blumeria graminis f. sp. hordei challenge and pre-challenge rhizosphere multi-omics assays.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Aeromicrobium ginsengisoli B1 — ontology label: Aeromicrobium ginsengisoli | NCBITaxon:363867 · TaxonMech · TraitMech | DOMINANT | |
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| Arthrobacter humicola B2 — ontology label: Arthrobacter humicola | NCBITaxon:409291 · TaxonMech · TraitMech | DOMINANT | |
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| Mesorhizobium ciceri B3 — ontology label: Mesorhizobium ciceri | NCBITaxon:39645 · TaxonMech · TraitMech | DOMINANT | |
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| Mycolicibacterium montmartrense B4 — ontology label: Mycolicibacterium montmartrense | NCBITaxon:1641990 · TaxonMech · TraitMech | DOMINANT | |
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| Phyllobacterium brassicacearum B5 — ontology label: Phyllobacterium brassicacearum | NCBITaxon:314235 · TaxonMech · TraitMech | DOMINANT | |
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| Phyllobacterium loti B6 — ontology label: Phyllobacterium loti | NCBITaxon:1334106 · TaxonMech · TraitMech | DOMINANT | |
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| Priestia aryabhattai B7 — ontology label: Priestia aryabhattai | NCBITaxon:412384 · TaxonMech · TraitMech | DOMINANT | |
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| Priestia megaterium B8 — ontology label: Priestia megaterium | NCBITaxon:1404 · TaxonMech · TraitMech | DOMINANT | |
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| Pseudomonas poae B9 — ontology label: Pseudomonas poae | NCBITaxon:200451 · TaxonMech · TraitMech | DOMINANT | |
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| Rhizobium alamii B10 — ontology label: Rhizobium alamii | NCBITaxon:492774 · TaxonMech · TraitMech | DOMINANT | |
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| Rhizobium viscosum B11 — ontology label: Rhizobium viscosum | NCBITaxon:1673 · TaxonMech · TraitMech | DOMINANT | |
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| Rhodococcus fascians B12 — ontology label: Rhodococcoides fascians | NCBITaxon:1828 · TaxonMech · TraitMech | DOMINANT | |
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| Streptomyces nigrescens B13 — ontology label: Streptomyces nigrescens | NCBITaxon:1920 · TaxonMech · TraitMech | DOMINANT | |
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| Streptomyces resistomycificus B14 — ontology label: Streptomyces resistomycificus | NCBITaxon:67356 · TaxonMech · TraitMech | DOMINANT | |
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| Streptomyces rishiriensis B15 — ontology label: Streptomyces rishiriensis | NCBITaxon:68264 · TaxonMech · TraitMech | DOMINANT | |
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| Streptomyces tauricus B16 — ontology label: Streptomyces tauricus | NCBITaxon:68274 · TaxonMech · TraitMech | DOMINANT | |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Barley DT-SynCom powdery mildew resistance
Barley seedlings root-inoculated with the barley-origin DT-SynCom showed lower Blumeria graminis f. sp. hordei DAF fluorescence intensity than mock-treated controls after pathogen challenge, consistent with SynCom-induced systemic resistance under the assay conditions.
Evidence
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PMID:42453107 - SUPPORT (IN_VIVO)"Mean fluorescence was reduced by 50.4% in the barley SynCom treatment, 43.3% in the wheat SynCom treatment, and 34.9% in the WCS417r treatment, relative to the mock treatment. "
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PMID:42453107 - SUPPORT (IN_VIVO)"These results demonstrate that both the barley and wheat SynComs induce ISR in a manner similar to that of the ISR-eliciting bacterium WCS417r. "
Barley DT-SynCom rhizosphere persistence
OTUs matching the 16S rDNA sequences of inoculated SynCom strains at least 99% identity and coverage were detected in barley rhizosphere samples at harvest, although the assay could not confirm strain-level identity.
Evidence
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PMID:42453107 - SUPPORT (IN_VIVO)"OTUs with ≥ 99% sequence identity to the 16S rRNA gene sequences of the inoculated SynCom strains were detected in rhizosphere samples at harvest "
Associated Datasets
| Dataset | Type | Repository | Accession |
|---|---|---|---|
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Barley DT-SynCom rhizosphere multi-omics BioProject PRJNA1405459
NCBI BioProject accession reported for the barley rhizosphere 16S rRNA gene amplicon, rhizosphere metatranscriptome, and host leaf RNA-seq data generated in the DT-SynCom powdery mildew resistance study. |
MULTI_OMICS | NCBI_BIOPROJECT | PRJNA1405459 |
Knowledge gaps & discussions (2)
KNOWLEDGE_GAP OPEN Which 16S NCBI accession belongs to Priestia aryabhattai B8W22 in the Barley DT-SynCom?
Table 1 prints NR_133818.1 for both the B6 Phyllobacterium loti row and the B7 Priestia aryabhattai B8W22 row. The record preserves the B7 accession as printed but does not correct the apparent duplicate without an independent source.
Attaches to: taxonomy
KNOWLEDGE_GAP OPEN When will BioProject PRJNA1405459 resolve publicly at NCBI?
The paper reports PRJNA1405459 for the generated sequencing data, but NCBI BioProject returned a "not public" placeholder for accession 1405459 during curation on 2026-09-24.
Attaches to: associated_datasets#PRJNA1405459
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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Primary publication - barley and wheat DT-SynComs
Frontiers in Microbiology 2026 open-access primary article, PMID:42453107 and PMCID:PMC13365038, describing barley- and wheat-origin DT-SynComs for barley powdery mildew resistance. |
OTHER | doi:10.3389/fmicb.2026.1818676 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| GCEF barley isolate source | Barley cultivar Antonella rhizosphere | N/A |
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| Equal-proportion barley strain mixture | 16 strains in 10 mM MgCl2 | N/A |
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| Barley powdery mildew challenge | Blumeria graminis f. sp. hordei | N/A |
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Growth Media
Cross-Mech Suite Links
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