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Bifidobacterium longum DSM20219 and Bacillus subtilis SOM8 Alginate Biofilm Probiotic Co-Culture

Community ID CommunityMech:000478
Ecological State ENGINEERED
Environment laboratory environment
ENVO:01001405 · HabitatMech ↗

Engineered probiotic co-culture of oxygen-sensitive anaerobic Bifidobacterium longum DSM20219 and biofilm-forming Bacillus subtilis SOM8 co-encapsulated in sodium alginate hydrogel beads. Bacillus subtilis SOM8 promotes interspecies cell aggregation, extracellular polymeric matrix development, and biofilm formation within the alginate matrix, enhancing B. longum DSM20219 survival by ~3-log CFU/g (>7-log CFU/g) in simulated gastric and intestinal fluids and maintaining >6-log CFU/g over 28 days of aerobic storage.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Bacillus subtilis NCBITaxon:1423 · TaxonMech · TraitMech
SYNTROPHIC_PARTNER
DOMINANT
  • PMID:42372892 - SUPPORT (IN_VITRO)
    "Here, we developed an alginate-based, biofilm-assisted encapsulation system for the co-culture and co-delivery of Bacillus subtilis SOM8 and Bifidobacterium longum DSM20219."
Bifidobacterium longum NCBITaxon:216816 · TaxonMech · TraitMech
SYNTROPHIC_PARTNER
DOMINANT
  • PMID:42372892 - SUPPORT (IN_VITRO)
    "Biofilm-assisted encapsulation improved B. longum DSM20219 viability, maintaining survival above 7-log CFU/g after 2 h in SGF and SIF, representing an approximately 3-log improvement over alginate-only encapsulation."

Ecological Interactions

The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.

Ecological interaction network for Bifidobacterium longum DSM20219 and Bacillus subtilis SOM8 Alginate Biofilm Probiotic Co-Culture Bipartite graph where circles represent taxa and non-circular symbols represent interactions, with distinct shapes and colours for mutualism. Rare or untyped interactions share the Other symbol; exact curated types remain in the interaction details.
Taxon
Mutualism
Other

Biofilm Formation and Extracellular Matrix Development Within Alginate Hydrogel

COMMENSALISM

Within alginate hydrogel beads, Bacillus subtilis SOM8 and Bifidobacterium longum DSM20219 engage in dynamic interspecies interactions that promote biofilm formation, cell aggregation, and extracellular matrix development.

Source Taxon: Bacillus subtilis

Target Taxon: Bifidobacterium longum

Biological Processes:

Downstream Effects:
→ Enhanced Probiotic Survival Against Simulated Gastric and Intestinal Fluid Stress

DIRECTLY IMPLIED: Biofilm formation, cell aggregation, and extracellular matrix development within the alginate co-encapsulation system protect Bifidobacterium longum DSM20219 against gastric acid and bile salt stress.

Evidence

  • PMID:42372892 - SUPPORT (IN_VITRO)
    "Imaging and time-resolved co-culture analyses suggested that the enhanced protection was associated with biofilm formation, cell aggregation, extracellular matrix development, and dynamic interspecies interactions within the alginate system."
  • PMID:42372892 - SUPPORT (IN_VITRO)
    "Here, we developed an alginate-based, biofilm-assisted encapsulation system for the co-culture and co-delivery of Bacillus subtilis SOM8 and Bifidobacterium longum DSM20219. This strategy enhanced probiotic tolerance to simulated gastric fluids (SGF) and simulated intestinal fluids (SIF), and extended shelf life under aerobic conditions."

Enhanced Probiotic Survival Against Simulated Gastric and Intestinal Fluid Stress

COMMENSALISM

Biofilm-assisted co-encapsulation with Bacillus subtilis SOM8 increases Bifidobacterium longum DSM20219 survival by approximately 3-log over alginate-only encapsulation, maintaining viability above 7-log CFU/g after 2 h in simulated gastric and intestinal fluids.

Source Taxon: Bacillus subtilis

Target Taxon: Bifidobacterium longum

Biological Processes:

Downstream Effects:
→ Extended Aerobic Storage Viability of Oxygen-Sensitive Bifidobacterium longum

DIRECTLY IMPLIED: Matrix and biofilm barrier formation within the Bacillus-Bifidobacterium alginate co-culture also protects oxygen-sensitive Bifidobacterium longum during long-term aerobic storage.

Evidence

  • PMID:42372892 - SUPPORT (IN_VITRO)
    "Biofilm-assisted encapsulation improved B. longum DSM20219 viability, maintaining survival above 7-log CFU/g after 2 h in SGF and SIF, representing an approximately 3-log improvement over alginate-only encapsulation."
  • PMID:42372892 - SUPPORT (IN_VITRO)
    "It also maintained over 6-log CFU/g after 28 days of aerobic storage."

Extended Aerobic Storage Viability of Oxygen-Sensitive Bifidobacterium longum

MUTUALISM

Alginate-based biofilm-assisted co-encapsulation of Bifidobacterium longum DSM20219 with Bacillus subtilis SOM8 preserves anaerobic probiotic viability above 6-log CFU/g across 28 days of ambient aerobic storage.

Source Taxon: Bifidobacterium longum

Target Taxon: Bacillus subtilis

Biological Processes:

Evidence

  • PMID:42372892 - SUPPORT (IN_VITRO)
    "It also maintained over 6-log CFU/g after 28 days of aerobic storage."

Environmental Factors

Factor Value Unit
Simulated Gastrointestinal Fluids and Aerobic Storage 2 h in SGF and SIF; 28 days aerobic storage N/A
  • PMID:42372892 - SUPPORT (IN_VITRO)
    "This strategy enhanced probiotic tolerance to simulated gastric fluids (SGF) and simulated intestinal fluids (SIF), and extended shelf life under aerobic conditions."

Cross-Mech Suite Links

Explore related entities across the CultureBotAI X-Mech knowledge bases:

HabitatMech: laboratory environment ↗ TaxonMech: Bacillus subtilis ↗ TraitMech: Bacillus subtilis ↗ TaxonMech: Bifidobacterium longum ↗ TraitMech: Bifidobacterium longum ↗ CultureMech Growth Media ↗ PathwayMech Pathways ↗