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Black-Soil Glucose-Activated Herbicide-Mixture Degrading Synthetic Community

Community ID CommunityMech:000482
Ecological State ENGINEERED
Environment contaminated soil
ENVO:00002116 · HabitatMech ↗

Simplified four-isolate bacterial synthetic community (SynCom) constructed from keystone taxa—Burkholderia-Caballeronia-Paraburkholderia complex, Rhodanobacter, and Achromobacter—identified in a natural microbial consortium enriched from herbicide-stressed agricultural black soil in Northeast China. Activated by glucose supplementation that fuels glycolytic and TCA cycle energy supply and environmental acidification, the SynCom co-metabolically degrades a recalcitrant mixture of nicosulfuron (97.27%), mesotrione (68.00%), and atrazine (22.91%) via enriched catabolic genes including atzF (allophanate hydrolase) and gst (glutathione S-transferase).

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Burkholderia NCBITaxon:32008 · TaxonMech · TraitMech
PRIMARY_DEGRADER SYNTROPHIC_PARTNER
DOMINANT
  • PMID:41762466 - SUPPORT (IN_VITRO)
    "Combined random forest (RF) and co-occurrence network analyses identified the Burkholderia-Caballeronia-Paraburkholderia complex, Rhodanobacter, and Achromobacter as the keystone taxa."
Rhodanobacter NCBITaxon:75309 · TaxonMech · TraitMech
PRIMARY_DEGRADER SYNTROPHIC_PARTNER
DOMINANT
  • PMID:41762466 - SUPPORT (IN_VITRO)
    "Combined random forest (RF) and co-occurrence network analyses identified the Burkholderia-Caballeronia-Paraburkholderia complex, Rhodanobacter, and Achromobacter as the keystone taxa."
Achromobacter NCBITaxon:222 · TaxonMech · TraitMech
PRIMARY_DEGRADER SYNTROPHIC_PARTNER
DOMINANT
  • PMID:41762466 - SUPPORT (IN_VITRO)
    "Combined random forest (RF) and co-occurrence network analyses identified the Burkholderia-Caballeronia-Paraburkholderia complex, Rhodanobacter, and Achromobacter as the keystone taxa."

Ecological Interactions

The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.

Ecological interaction network for Black-Soil Glucose-Activated Herbicide-Mixture Degrading Synthetic Community Bipartite graph where circles represent taxa and non-circular symbols represent interactions, with distinct shapes and colours for syntrophy.
Taxon
Syntrophy

Glucose-Activated Central Carbon Metabolism and Environmental Acidification

SYNTROPHY

Glucose supplementation activates glycolytic and tricarboxylic acid (TCA) cycle central carbon metabolism in the black-soil consortium, enhancing cellular energy supply and environmental acidification required for co-metabolic herbicide degradation.

Participating Taxa: Burkholderia, Rhodanobacter, Achromobacter

Metabolites: glucose (CHEBI:17234 · MediaIngredientMech · CMMMech), atrazine (CHEBI:15930 · MediaIngredientMech · CMMMech)

Biological Processes:

Downstream Effects:
→ Keystone Degrader Enrichment of Allophanate Hydrolase (atzF) and Glutathione S-Transferase (gst)

DIRECTLY IMPLIED: Glucose-driven central carbon activation and community restructuring enrich keystone Burkholderia, Rhodanobacter, and Achromobacter degraders carrying atzF and gst herbicide-catabolic genes.

Evidence

  • PMID:41762466 - SUPPORT (IN_VITRO)
    "Integrated 16S rRNA gene sequencing and metagenomic analysis linked the glucose amendment to a specific shift in the microbial community structure and activation of central carbon metabolism"
  • PMID:41762466 - SUPPORT (IN_VITRO)
    "Combined random forest (RF) and co-occurrence network analyses identified the Burkholderia-Caballeronia-Paraburkholderia complex, Rhodanobacter, and Achromobacter as the keystone taxa."

Keystone Degrader Enrichment of Allophanate Hydrolase (atzF) and Glutathione S-Transferase (gst)

SYNTROPHY

Glucose-activated community assembly enriches keystone genera Burkholderia, Rhodanobacter, and Achromobacter harboring herbicide-degrading genes including atzF (allophanate hydrolase) and gst (glutathione S-transferase).

Participating Taxa: Burkholderia, Rhodanobacter, Achromobacter

Metabolites: glucose (CHEBI:17234 · MediaIngredientMech · CMMMech), atrazine (CHEBI:15930 · MediaIngredientMech · CMMMech)

Biological Processes:

Downstream Effects:
→ Synergistic Four-Isolate SynCom Herbicide-Mixture Mineralization

DIRECTLY IMPLIED: Functional association of the enriched keystone degraders enables a constructed four-isolate SynCom to co-metabolically degrade the atrazine-nicosulfuron-mesotrione mixture more efficiently than single isolates or the parent consortium.

Evidence

  • PMID:41762466 - SUPPORT (IN_VITRO)
    "Metagenomic screening showed that these taxa were enriched for functional genes associated with herbicide degradation, including atzF (allophanate hydrolase) and gst (glutathione S-transferase)."
  • PMID:41762466 - SUPPORT (IN_VITRO)
    "A simplified four-isolate SynCom, constructed based on these functional associations, degraded the herbicide mixtures more efficiently than either the individual isolates or the NMC in the GSM system."

Synergistic Four-Isolate SynCom Herbicide-Mixture Mineralization

SYNTROPHY

A simplified four-isolate synthetic community constructed from the keystone genera degrades the nicosulfuron-mesotrione-atrazine mixture more efficiently than individual isolates or the parent natural consortium under glucose supplementation.

Participating Taxa: Burkholderia, Rhodanobacter, Achromobacter

Metabolites: glucose (CHEBI:17234 · MediaIngredientMech · CMMMech), atrazine (CHEBI:15930 · MediaIngredientMech · CMMMech)

Biological Processes:

Evidence

  • PMID:41762466 - SUPPORT (IN_VITRO)
    "A simplified four-isolate SynCom, constructed based on these functional associations, degraded the herbicide mixtures more efficiently than either the individual isolates or the NMC in the GSM system."

Environmental Factors

Factor Value Unit
Glucose Co-Substrate Supplementation and Herbicide Mixture Glucose-supplemented mineral salt medium (GSM) with atrazine, nicosulfuron, and mesotrione N/A
  • PMID:41762466 - SUPPORT (IN_VITRO)
    "Significant herbicide degradation occurred only in the GSM system, with degradation rates of 97.27% for nicosulfuron, 68.00% for mesotrione, and 22.91% for atrazine after 8 days."

Cross-Mech Suite Links

Explore related entities across the CultureBotAI X-Mech knowledge bases:

HabitatMech: contaminated soil ↗ TaxonMech: Burkholderia ↗ TraitMech: Burkholderia ↗ TaxonMech: Rhodanobacter ↗ TraitMech: Rhodanobacter ↗ TaxonMech: Achromobacter ↗ TraitMech: Achromobacter ↗ CultureMech Growth Media ↗ PathwayMech Pathways ↗