A defined two-strain consortium reconstructed from the dimethachlon-enriched community JHJ-2. Bosea sp. S6 transforms dimethachlon into the toxic intermediate 3,5-dichloroaniline, and Pseudomonas sp. KH-1 consumes that intermediate, completing a sequential degradation and detoxification handoff.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Bosea sp. S6 — ontology label: Allobosea | NCBITaxon:85413 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
|
N/A |
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| Pseudomonas sp. KH-1 — ontology label: Pseudomonas | NCBITaxon:286 · TaxonMech · TraitMech |
CROSS_FEEDER
PRIMARY_DEGRADER
|
N/A |
|
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Sequential dimethachlon-metabolite handoff
CROSS_FEEDINGThe source reports that S6 transforms dimethachlon to 3,5-dichloroaniline and KH-1 degrades that intermediate. This sequential substrate handoff supports cross-feeding, not necessarily mineralization, reciprocal fitness benefit or proven necessity of particular enzymes.
Source Taxon: Bosea sp. S6
Target Taxon: Pseudomonas sp. KH-1
Metabolites: 3,5-dichloroaniline (CHEBI:19904 · MediaIngredientMech · CMMMech)
PARTIAL - S6 production and KH-1 consumption of 3,5-dichloroaniline provide a proposed route enabling paired parent/intermediate removal in soil. The abstract reports both strain functions and bioaugmentation outcome, but does not resolve in-soil flux or strain/mediator necessity.
Evidence
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PMID:41326129 - SUPPORT (IN_VITRO)"Bosea sp. S6, which transforms dimethachlon to 3,5-dichloroaniline, and Pseudomonas sp. KH-1, which degrades 3,5-dichloroaniline"
Complete soil removal of dimethachlon and 3,5-dichloroaniline
Bioaugmentation with the two-strain consortium was reported to completely remove dimethachlon and 3,5-dichloroaniline from contaminated soil under the tested conditions. Removal does not establish mineralization or toxicity of the resulting soil samples, and enrichment-culture JHJ-2 kinetics are not assigned to this pair.
Participating Taxa: Bosea sp. S6, Pseudomonas sp. KH-1
Metabolites: 3,5-dichloroaniline (CHEBI:19904 · MediaIngredientMech · CMMMech)
Evidence
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PMID:41326129 - SUPPORT (IN_VITRO)"Bioaugmentation with the synthetic consortium led to the complete removal of dimethachlon and its highly toxic metabolite 3,5-dichloroaniline from contaminated soil."
Detoxified dimethachlon transformation products
Products of the two-strain dimethachlon treatment were reported as non-toxic in a zebrafish assay. The available abstract/preview does not resolve exposure stage, dose, endpoint or linkage to the soil-remediation samples. This is an assay-bounded outcome, not universal safety or an independently demonstrated cross-feeding event.
Participating Taxa: Bosea sp. S6, Pseudomonas sp. KH-1
Metabolites: 3,5-dichloroaniline (CHEBI:19904 · MediaIngredientMech · CMMMech)
Evidence
-
PMID:41326129 - SUPPORT (OTHER)"Toxicity assays using the zebrafish showed that dimethachlon is converted into non-toxic products by the synthetic consortium (strains S6 and KH-1)."
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Do full methods connect the degradation, soil-removal and zebrafish endpoints?
Complete abstract and selected publisher preview were read, not full methods, images or supplements. The soil-to-toxicity arrow lacked verified sample linkage and was removed. Strain-specific handoff is retained with bounded soil mediation. Recover organism stage, exposure and endpoint details before assigning toxicity context; follow-up #1268. Preserve Allobosea canonical grounding and GTDB Bosea mapping.
Attaches to: ecological_interactions#Sequential dimethachlon-metabolite handoff, ecological_interactions#Complete soil removal of dimethachlon and 3,5-dichloroaniline, ecological_interactions#Detoxified dimethachlon transformation products
External Resources
| Name | Repository | Resource ID |
|---|---|---|
|
Primary publication - S6 and KH-1 dimethachlon consortium
Primary construction and functional study of the exact two-strain consortium. |
OTHER | PMID:41326129 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Complete contaminant and metabolite removal in soil | complete removal | N/A |
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| Transformation-product toxicity | non-toxic in zebrafish assay | N/A |
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