An operated anammox biofilter community subjected to sustained low-substrate nitrogen stress, where metagenomics, metatranscriptomics, co-occurrence networks, and SMETANA supported functional succession from Ca. Kuenenia toward a multispecies, transcriptionally active Ca. Brocadia assemblage with lower modeled resource overlap and complementary auxotrophies.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Ca. Brocadia low-nitrogen assemblage — ontology label: Candidatus Brocadia | NCBITaxon:380240 · TaxonMech · TraitMech |
PRIMARY_PRODUCER
CROSS_FEEDER
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DOMINANT |
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| Ca. Kuenenia early-acclimation lineage — ontology label: Candidatus Kuenenia | NCBITaxon:380738 · TaxonMech · TraitMech |
PRIMARY_PRODUCER
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N/A |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Brocadia complementary low-nitrogen auxotrophy
CROSS_FEEDINGUnder sustained low-nitrogen biofilter operation, functional succession replaced the early-acclimation Ca. Kuenenia lineage (which downregulated costly aromatic amino-acid biosynthesis by 25.6%-38.8%) with a transcriptionally activated multispecies Ca. Brocadia assemblage characterized by SMETANA-inferred complementary auxotrophies, consistent with potential cooperation rather than dominance by one superior anammox competitor.
Participating Taxa: Ca. Brocadia low-nitrogen assemblage, Ca. Kuenenia early-acclimation lineage
Evidence
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PMID:42323145 - SUPPORT (COMPUTATIONAL)"Ultimately, Ca. Brocadia replaced Ca. Kuenenia as the dominant functional lineage"
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PMID:42323145 - SUPPORT (COMPUTATIONAL)"characterized by complementary model-inferred auxotrophies"
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Which exact Ca. Brocadia species or MAGs composed the multispecies low-nitrogen assemblage, and what reactor geometry, influent nitrogen concentrations, and loading rates produced the succession?
The PubMed abstract establishes genus-level succession and model-inferred Brocadia cooperation, but the article was not open-access through Europe PMC and the abstract does not expose species-level MAG identities, the source of the SMETANA genome-scale models, or reproducible biofilter operating parameters.
Attaches to: taxonomy#Ca. Brocadia low-nitrogen assemblage, environmental_factors#Sustained low-substrate nitrogen stress
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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PubMed record
PubMed record for the Bioresource Technology primary article on low-nitrogen anammox succession toward Ca. Brocadia. |
OTHER | PMID:42323145 |
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Article DOI
DOI for the Bioresource Technology primary article reporting low-substrate nitrogen acclimation in an anammox biofilter. |
OTHER | doi:10.1016/j.biortech.2026.135206 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Sustained low-substrate nitrogen stress | N/A | |
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| Ca. Brocadia transcriptional activation | about 18-fold anammox-gene upregulation | N/A |
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| Lower modeled metabolic resource overlap | 0.66 +/- 0.12 versus 0.77 +/- 0.10 in the high-nitrogen system | N/A |
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Cross-Mech Suite Links
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