A defined two-strain synthetic consortium, YB2, constructed from Burkholderia cepacia ZY1 and Pseudomonas harudinis G1B at an optimized 4:5 inoculation ratio to improve polyethylene terephthalate (PET) depolymerization and downstream PET-hydrolysis-product conversion. At pH 7.0 and 30 °C, YB2 reached 4.30% +/- 0.11% PET film mass loss after 3 days, outperformed the individual strains, released BHET, MHET, TPA, and ethylene glycol, and converted 96.4% of BHET plus 97.3% of MHET within 2 days.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Burkholderia cepacia ZY1 — ontology label: Burkholderia cepacia | NCBITaxon:292 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
|
N/A |
|
|||
| Pseudomonas harudinis G1B — ontology label: Pseudomonas harudinis | NCBITaxon:2833596 · TaxonMech · TraitMech |
CROSS_FEEDER
|
N/A |
|
|||
Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
YB2 PET hydrolysis-product complementarity
B. cepacia ZY1 and P. harudinis G1B showed overlapping PET-related functions with relative differences: stronger ZY1 depolymerization-related activity and better G1B growth in BHET/MHET media. PARTIAL - the authors propose potential complementarity, not demonstrated resource partitioning, reciprocal benefit or donor-resolved cross-feeding.
Participating Taxa: Burkholderia cepacia ZY1, Pseudomonas harudinis G1B
Metabolites: poly(ethylene terephthalate) macromolecule (CHEBI:53259 · MediaIngredientMech · CMMMech), bis(2-hydroxyethyl) terephthalate (CHEBI:231672 · MediaIngredientMech · CMMMech), terephthalic acid (CHEBI:15702 · MediaIngredientMech · CMMMech)
Biological Processes:
- xenobiotic metabolic process (GO:0006805 · PathwayMech)
PARTIAL - relative depolymerization and downstream-conversion capacities provide a proposed explanation for greater YB2 PET-film mass loss. The abstract does not isolate functional necessity or trace interstrain transfer, so this remains a source-supported mechanism hypothesis.
Evidence
-
PMID:42664909 - PARTIAL (IN_VITRO)"Overall, ZY1 and G1B exhibited overlapping functions with relative functional differences and potential complementarity "
YB2 enhanced PET-film degradation
At pH 7.0 and 30 C, YB2 achieved 4.30% +/- 0.11% PET-film mass loss after three days, exceeding the individual strains, with surface erosion, ester-related changes and increased residual crystallinity. These endpoints are not mineralization or proof of niche partitioning. BHET/MHET measurements at two days and TPA measurements at five days are separate time points, not the three-day mass-loss assay.
Participating Taxa: Burkholderia cepacia ZY1, Pseudomonas harudinis G1B
Metabolites: poly(ethylene terephthalate) macromolecule (CHEBI:53259 · MediaIngredientMech · CMMMech)
Biological Processes:
- xenobiotic metabolic process (GO:0006805 · PathwayMech)
Evidence
-
PMID:42664909 - SUPPORT (IN_VITRO)"At pH 7.0 and 30 °C, YB2 achieved 4.30% ± 0.11% PET film mass loss after 3 d, significantly exceeding the individual strains. "
-
PMID:42664909 - SUPPORT (IN_VITRO)"YB2 treatment caused surface erosion, ester-related structural changes, and increased residual PET crystallinity. "
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Does selective loss of a partner function or traced exchange explain enhanced PET-film mass loss?
Complete abstract only, not full methods or supplements. Hydrolysis was predominantly cell-associated; both strains remained culturable over 0-7 days, which does not establish mutualism or obligation. Ethylene-glycol decline suggests possible use, not tested assimilation. Preserve current taxon/chemical groundings; non-graph G1B cross-feeder role remains #1268.
Attaches to: ecological_interactions#YB2 PET hydrolysis-product complementarity, ecological_interactions#YB2 enhanced PET-film degradation
External Resources
| Name | Repository | Resource ID |
|---|---|---|
|
Primary publication - YB2 Burkholderia-Pseudomonas PET consortium
Journal of Environmental Management primary article describing the YB2 Burkholderia cepacia ZY1 and Pseudomonas harudinis G1B two-strain consortium for enhanced PET degradation. |
OTHER | PMID:42664909 |
|
||
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Optimized ZY1 to G1B inoculation ratio | 4:5 | N/A |
|
||
| PET film mass loss at pH 7.0 and 30 C | 4.30 +/- 0.11 | percent after 3 days |
|
||
| BHET and MHET conversion | 96.4% BHET and 97.3% MHET within 2 days | N/A |
|
||
Growth Media
Cross-Mech Suite Links
Explore related entities across the CultureBotAI X-Mech knowledge bases: