A synthetic lignocellulolytic bacterial consortium designed from six bovine rumen microbial strains to enhance lignocellulose degradation for biofuel and enzyme production. Genome-scale community metabolic models were used with flux-based parameters (pairwise metabolic assistance, PMA; growth support index, PGSI) to predict compatible, mutualistic pairs, which were validated in experimentally grown cocultures. The strongest pairwise enzymatic synergism was observed for Butyrivibrio fibrisolvens with Selenomonas ruminantium (a 41% increase in endoglucanase activity), and a three-membered community of B. fibrisolvens, S. ruminantium, and Ruminococcus albus showed synergistic activity across all lignocellulosic substrates, indicating strong metabolic interplay among compatible species.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Butyrivibrio fibrisolvens | NCBITaxon:831 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
|
N/A |
|
|||
| Selenomonas ruminantium — ontology label: Pseudoselenomonas ruminantium | NCBITaxon:971 · TaxonMech · TraitMech |
SECONDARY_FERMENTER
CROSS_FEEDER
|
N/A |
|
|||
| Ruminococcus albus — ontology label: Hominimerdicola alba | NCBITaxon:1264 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
|
N/A |
|
|||
Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Enzymatic synergism in the B. fibrisolvens + S. ruminantium coculture
The tested B. fibrisolvens and S. ruminantium pair showed the strongest reported enzymatic synergism and a 41% increase in endoglucanase activity. The accessed abstract does not define the percentage denominator. Enzyme activity does not demonstrate positive fitness effects for both partners, so MUTUALISM is not assigned.
Source Taxon: Butyrivibrio fibrisolvens
Target Taxon: Selenomonas ruminantium
Biological Processes:
- cellulose catabolic process (GO:0030245 · PathwayMech)
Evidence
-
PMID:42343824 - SUPPORT (IN_VITRO)"The highest degree of enzymatic synergism has been achieved through the pairwise coculture of Butyrivibrio fibrisolvens and Selenomonas ruminantium, resulting in a 41% increase in the endoglucanase activity."
Three-membered lignocellulolytic synergy
The three-member B. fibrisolvens, S. ruminantium and R. albus community exhibited synergistic lignocellulolytic activity across all tested substrates. PARTIAL - metabolic interplay is the authors' interpretation, not resolved exchange or reciprocal partner fitness. This endpoint is distinct from the model-screening and prediction-validation workflow.
Participating Taxa: Butyrivibrio fibrisolvens, Selenomonas ruminantium, Ruminococcus albus
Biological Processes:
- cellulose catabolic process (GO:0030245 · PathwayMech)
Evidence
-
PMID:42343824 - SUPPORT (IN_VITRO)"a combination of B. fibrisolvens, S. ruminantium, and Ruminococcus albus exhibited synergistic activity across all lignocellulosic substrates, suggesting the strong metabolic interplay between compatible species"
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Which exchanges and partner responses explain the reported enzymatic synergism?
Complete abstract read, not full methods, images or supplements. PMA-PGSI screening and correlating predictions with experiments are analytical workflows, so two nodes were removed; earlier workflow arrows stay absent. The 41% denominator and exact experimental controls require full methods. Preserve #1217 Pseudoselenomonas ruminantium and Hominimerdicola alba groundings. Non-graph partner roles remain #1268.
Attaches to: ecological_interactions#Enzymatic synergism in the B. fibrisolvens + S. ruminantium coculture, ecological_interactions#Three-membered lignocellulolytic synergy
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