CRC Fusobacterium Control SynCom

Community ID CommunityMech:000128

Ecological State ENGINEERED

Environment colorectal gut microbiome
UBERON:0001155

A bottom-up designed seven-species synthetic microbial community for ecological control of Fusobacterium nucleatum-associated colorectal cancer models.

Taxonomy

Taxon	Ontology ID	Functional Roles	Abundance
seven commensal bacterial SynCom members	NCBITaxon:2	CROSS_FEEDER	N/A
PMID:40433987 - SUPPORT (IN_VIVO) "A synthetic microbial community (SynCom) is then designed by metabolic network reconstruction, and its performance is validated using coculture experiments and an AOM-DSS induced mouse CRC model."

Ecological Interactions

Taxon

Competition

Fusobacterium Ecological Suppression

COMPETITION

Evidence

PMID:40433987 - SUPPORT (IN_VIVO)

"A synthetic microbial community (SynCom) is then designed by metabolic network reconstruction, and its performance is validated using coculture experiments and an AOM-DSS induced mouse CRC model."

Environmental Factors

Factor	Value	Unit
metabolic network reconstruction design	A seven-species SynCom with low competitive interrelationships was designed from human metagenome and metabolomics data.	N/A
PMID:40433987 - SUPPORT (IN_VIVO) "A synthetic microbial community (SynCom) is then designed by metabolic network reconstruction, and its performance is validated using coculture experiments and an AOM-DSS induced mouse CRC model."

Growth Media

GAM medium (Gifu Anaerobic Medium)

Atmosphere ANAEROBIC

Preparation notes: Seven-species SynCom and F. nucleatum cultured in GAM under anaerobic conditions; modified GAM (mGAM, GAM supplemented with arginine and tryptophan) and 1% arginine/tryptophan-supplemented GAM used across multiple transfer generations to test pH-dependent F. nucleatum suppression.

Evidence

PMID:40433987

"The nine species were first cultured in vitro under anaerobic condition"

PMID:40433987

"growth curves of SynCom in GAM medium"