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Caldicellulosiruptor Two-Species Hydrogen Coculture

Community ID CommunityMech:000185
Ecological State ENGINEERED
Environment thermophilic anaerobic continuous-flow laboratory bioreactor
ENVO:01001405

A de novo constructed, continuous-flow, hydrogen-producing coculture pairing Caldicellulosiruptor saccharolyticus DSM 8903 with the strain reported as Caldicellulosiruptor kristjanssonii DSM 12137. The system was designed to test whether two extreme thermophilic sugar-fermenting Caldicellulosiruptor strains could stably coexist while converting glucose and xylose, major lignocellulosic hydrolysate sugars, to hydrogen-rich fermentation products.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Caldicellulosiruptor saccharolyticus DSM 8903 NCBITaxon:351627
SECONDARY_FERMENTER CROSS_FEEDER
N/A
  • PMID:21192828 - SUPPORT (IN_VITRO)
    "Caldicellulosiruptor saccharolyticus DSM 8903"
Caldicellulosiruptor kristjanssonii DSM 12137 NCBITaxon:632335
SECONDARY_FERMENTER CROSS_FEEDER
N/A
  • PMID:21192828 - SUPPORT (IN_VITRO)
    "C. kristjanssonii DSM 12137"

Ecological Interactions

Ecological interaction network for Caldicellulosiruptor Two-Species Hydrogen Coculture Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

Stable Chemostat Coexistence

MUTUALISM

Source Taxon: Caldicellulosiruptor saccharolyticus DSM 8903

Target Taxon: Caldicellulosiruptor kristjanssonii DSM 12137

Metabolites: glucose (CHEBI:17234), xylose (CHEBI:18222)

Biological Processes:

  • interspecies interaction between organisms (GO:0044419)

Evidence

  • PMID:21192828 - SUPPORT (IN_VITRO)
    "co-culture exhibited a remarkable stability over a period of 70 days"
  • PMID:21192828 - SUPPORT (IN_VITRO)
    "The two strains retained their ability to stably coexist"

C. saccharolyticus Growth-Supernatant Enhancement

COLONIZATION_FACILITATION

Source Taxon: Caldicellulosiruptor saccharolyticus DSM 8903

Target Taxon: Caldicellulosiruptor kristjanssonii DSM 12137

Biological Processes:

  • interspecies interaction between organisms (GO:0044419)

Evidence

  • PMID:21192828 - SUPPORT (IN_VITRO)
    "enhance the growth of C. kristjanssonii"
  • PMID:21192828 - SUPPORT (IN_VITRO)
    "increasing its maximum biomass concentration by ca. 18%"

Synergistic Biohydrogen Production

CROSS_FEEDING

Source Taxon: Caldicellulosiruptor saccharolyticus DSM 8903

Target Taxon: Caldicellulosiruptor kristjanssonii DSM 12137

Metabolites: hydrogen (CHEBI:18276), acetate (CHEBI:30089), glucose (CHEBI:17234), xylose (CHEBI:18222)

Biological Processes:

Evidence

  • PMID:21192828 - SUPPORT (IN_VITRO)
    "from glucose and xylose"
  • PMID:21192828 - SUPPORT (IN_VITRO)
    "H2 yields on glucose exceeded those of either organism alone"

Associated Datasets

Dataset Type Repository Accession
C. saccharolyticus DSM 8903 genome BioProject
Genome project for C. saccharolyticus DSM 8903, one member of the coculture. 		GENOME NCBI_BIOPROJECT PRJNA13466
C. kristjanssonii DSM 12137 genome BioProject
Genome project for the strain reported as C. kristjanssonii DSM 12137. 		GENOME NCBI_BIOPROJECT PRJNA41727
Coculture fermentation supplementary tables
Supplementary fermentation tables for product yields, sugar consumption, and carbon/electron recoveries. 		PHENOTYPE OTHER PMID:21192828-supplement-2

External Resources

Name Repository Resource ID
Exact-system primary publication - stable Caldicellulosiruptor coculture
Primary open-access study of the DSM 8903 and DSM 12137 hydrogen-producing coculture in continuous culture. 		OTHER PMID:21192828
  • PMID:21192828 - SUPPORT (IN_VITRO)
    "Stable coexistence of two Caldicellulosiruptor species"
Earlier exact-pair mixed-sugar coculture publication
Earlier publication evaluating C. saccharolyticus and C. kristjanssonii in coculture during mixed-sugar hydrogen production. 		OTHER doi:10.1016/j.ijhydene.2008.07.092

Environmental Factors

Factor Value Unit
Extreme thermophilic cultivation extreme thermophiles N/A
Mixed lignocellulosic sugars glucose and xylose N/A
Glucose-limited high-yield condition 3.7 mol H2 per mol glucose
  • PMID:21192828 - SUPPORT (IN_VITRO)
    "maximum H2 yield of 3.7 mol (mol glucose)(-1)"
  • PMID:21192828 - SUPPORT (IN_VITRO)
    "at a dilution rate of 0.06 h(-1)"

Growth Media

Glucose and xylose continuous-flow reactor feed

Atmosphere ANAEROBIC

Composition

Component Concentration Unit CHEBI
glucose N/A N/A CHEBI:17234
xylose N/A N/A CHEBI:18222

Preparation notes: The abstract supports glucose and xylose feed in a continuous-flow stirred tank reactor; detailed medium salts are not asserted here.

Evidence

PMID:21192828

"from glucose and xylose in a continuous-flow stirred tank reactor"

PMID:21192828

"glucose was used as the sole growth-limiting substrate"