A two-member fungal solid-state fermentation system consisting of Phanerochaete chrysosporium and Pleurotus eryngii that was constructed to convert the recalcitrant lignin fraction of Chinese Distillers' grains. Chen et al. 2026 measured 58.66% lignin degradation in Chinese Distillers' grains, day-10 peaks in laccase and manganese peroxidase activity, a day-15 peak in lignin peroxidase activity, and transcriptomic/metabolomic evidence that P. chrysosporium was most active in early oxidative lignin cleavage while P. eryngii became dominant during later aromatic catabolism.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Phanerochaete chrysosporium — ontology label: Phanerodontia chrysosporium | NCBITaxon:2822231 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
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N/A |
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| Pleurotus eryngii | NCBITaxon:5323 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
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N/A |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Temporal Dual-Fungal Lignin Conversion
NICHE_PARTITIONINGPARTIAL - transcriptomic and metabolomic patterns support the authors' proposed temporal division of lignin-conversion functions: early Phanerodontia chrysosporium AA2 peroxidase expression and later Pleurotus eryngii AA1 laccase/aromatic-catabolic expression. NICHE_PARTITIONING denotes this functional-staging interpretation, not measured population dominance, reduced competition or traced interpartner transfer. Enzyme-specific carbon flux is not resolved by the accessible abstract and primary preview.
Metabolites: lignin (CHEBI:6457 · MediaIngredientMech · CMMMech)
Biological Processes:
- lignin catabolic process (GO:0046274 · PathwayMech)
Evidence
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PMID:42660361 - PARTIAL (IN_VITRO)"Integrated transcriptomic and metabolomic analyses revealed a clear temporal division of labor between the dual-fungal."
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PMID:42660361 - PARTIAL (IN_VITRO)"During the early stage, P. chrysosporium predominantly expressed AA2 family peroxidase genes, promoting oxidative cleavage of lignin β-O-4 linkages"
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PMID:42660361 - PARTIAL (IN_VITRO)"P. eryngii became dominant during the middle and late stages through the upregulation of AA1 family laccase genes and aromatic catabolic pathways"
CDG Lignin Degradation by Dual-Fungal Fermentation
The dual-fungal solid-state culture reached 58.66% lignin degradation. Laccase and manganese peroxidase activities peaked at day 10; lignin peroxidase peaked at day 15. These bulk endpoints do not independently establish niche partitioning or identify each organism's contribution.
Metabolites: lignin (CHEBI:6457 · MediaIngredientMech · CMMMech)
Biological Processes:
- lignin catabolic process (GO:0046274 · PathwayMech)
Evidence
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PMID:42660361 - SUPPORT (IN_VITRO)"The lignin degradation ratio reached 58.66%, indicating that the system can effectively convert lignin."
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PMID:42660361 - SUPPORT (IN_VITRO)"Laccase (Lac) and manganese peroxidase (MnP) activities peaked at 10 d"
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PMID:42660361 - SUPPORT (IN_VITRO)"lignin peroxidase (LiP) reached its maximum activity (51.67 U/g) at 15 d."
Knowledge gaps & discussions (2)
KNOWLEDGE_GAP OPEN Which P. chrysosporium and P. eryngii strains, inoculation ratio, moisture content, temperature, aeration state, fermentation duration, and omics accessions were used to construct and analyze the CDG dual-fungal solid-state fermentation?
The accessible PubMed abstract names the two fungal species, CDGs as the solid lignin-rich substrate, ligninolytic enzyme timing, and transcriptomic/metabolomic evidence for temporal division of labor, but it does not report strain designations, inoculation ratio, moisture content, incubation temperature, aeration state, total fermentation duration, or omics accessions.
Attaches to: engineering_design, ecological_interactions#Temporal Dual-Fungal Lignin Conversion
KNOWLEDGE_GAP OPEN Do partner-resolved perturbations or tracing validate the proposed temporal division of labor?
PMID:42660361 supplies bulk degradation, enzyme timing and an omics-based functional-staging interpretation. Full methods/results, figure images and supplements remain unread. Preserve the existing strains/conditions gap; temporal expression is not population succession, traced transfer or experimental evidence of reduced resource competition.
Attaches to: ecological_interactions#Temporal Dual-Fungal Lignin Conversion, ecological_interactions#CDG Lignin Degradation by Dual-Fungal Fermentation
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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Primary publication - CDG dual-fungal lignin consortium
PubMed record for the Bioresource Technology study of the P. chrysosporium and P. eryngii dual-fungal solid-state fermentation system for lignin conversion in Chinese Distillers' grains. |
OTHER | PMID:42660361 |
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Publication DOI - CDG dual-fungal lignin consortium
DOI for the primary Bioresource Technology study of the dual-fungal CDG lignin consortium. |
OTHER | doi:10.1016/j.biortech.2026.135734 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Chinese Distillers' grains solid substrate | lignin-rich Chinese Distillers' grains | N/A |
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