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Clostridium-Caldicellulosiruptor Minimal Medium Coculture

Community ID CommunityMech:000193
Ecological State ENGINEERED
Environment thermophilic anaerobic laboratory bioreactor
ENVO:01001405

A thermophilic, anaerobic, two-member laboratory coculture pairing Clostridium thermocellum ATCC 27405 with Caldicellulosiruptor bescii DSM 6725. The system was used to test whether low-ionic-strength defined medium with reduced nitrogen, sulfur, phosphate, and vitamin supplements could maintain both cellulolytic bacterial populations during conversion of cellulosic substrates relevant to consolidated bioprocessing.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Clostridium thermocellum ATCC 27405 NCBITaxon:203119
PRIMARY_DEGRADER SECONDARY_FERMENTER
N/A
  • PMID:23306120 - SUPPORT (IN_VITRO)
    "Clostridium thermocellum ATCC 27405"
  • PMID:23306120 - SUPPORT (IN_VITRO)
    "cellulolytic Clostridium thermocellum ATCC 27405"
Caldicellulosiruptor bescii DSM 6725 NCBITaxon:521460
PRIMARY_DEGRADER SECONDARY_FERMENTER
N/A
  • PMID:23306120 - SUPPORT (IN_VITRO)
    "Caldicellulosiruptor bescii bacteria"
  • PMID:21227922 - SUPPORT (COMPUTATIONAL)
    "Caldicellulosiruptor bescii DSM 6725 utilizes various polysaccharides"

Ecological Interactions

Ecological interaction network for Clostridium-Caldicellulosiruptor Minimal Medium Coculture Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

Stable Co-Maintenance On Cellulose And Xylan

NICHE_PARTITIONING

Source Taxon: Clostridium thermocellum ATCC 27405

Target Taxon: Caldicellulosiruptor bescii DSM 6725

Metabolites: cellulose (CHEBI:18246), xylan (CHEBI:37686)

Biological Processes:

  • biological process involved in interspecies interaction between organisms (GO:0044419)
  • cellulose catabolic process (GO:0030245)

Evidence

  • PMID:23306120 - SUPPORT (IN_VITRO)
    "Both bacterial populations were maintained in co-cultures"
  • PMID:23306120 - SUPPORT (IN_VITRO)
    "substrates containing cellulose and xylan"

Thermophilic Plant-Polysaccharide Conversion Capacity

NICHE_PARTITIONING

Source Taxon: Caldicellulosiruptor bescii DSM 6725

Target Taxon: Clostridium thermocellum ATCC 27405

Metabolites: cellulose (CHEBI:18246), xylan (CHEBI:37686)

Biological Processes:

Evidence

  • PMID:23306120 - SUPPORT (IN_VITRO)
    "C. thermocellum was grown on crystalline cellulose"
  • PMID:21227922 - SUPPORT (COMPUTATIONAL)
    "overproduced on cellulose or xylan"

Associated Datasets

Dataset Type Repository Accession
C. thermocellum ATCC 27405 genome BioProject
Genome project for the C. thermocellum ATCC 27405 strain used in the coculture study. 		GENOME NCBI_BIOPROJECT PRJNA314
C. bescii DSM 6725 genome BioProject
Genome project for C. bescii DSM 6725, the second strain in the coculture study. 		GENOME NCBI_BIOPROJECT PRJNA29407

External Resources

Name Repository Resource ID
Exact-system primary publication - defined medium coculture
Primary study reporting defined minimal nutrient medium and maintenance of both C. thermocellum ATCC 27405 and C. bescii populations in coculture. 		OTHER PMID:23306120
  • PMID:23306120 - SUPPORT (IN_VITRO)
    "Both bacterial populations were maintained in co-cultures"
C. bescii DSM 6725 biomass-conversion genome paper
Genome and expression analysis supporting C. bescii DSM 6725 polysaccharide utilization and biomass-conversion capacity. 		OTHER PMID:21227922
  • PMID:21227922 - SUPPORT (COMPUTATIONAL)
    "Caldicellulosiruptor bescii DSM 6725 utilizes various polysaccharides"
C. thermocellum ATCC 27405 BioProject
NCBI BioProject for the genome-sequenced C. thermocellum ATCC 27405 strain. 		OTHER bioproject:PRJNA314
C. bescii DSM 6725 BioProject
NCBI BioProject for the genome-sequenced C. bescii DSM 6725 strain. 		OTHER bioproject:PRJNA29407

Environmental Factors

Factor Value Unit
Reduced-nutrient defined medium low ionic strength medium with decreased nitrogen, sulfur, phosphate, and vitamins N/A
  • PMID:23306120 - SUPPORT (IN_VITRO)
    "decreased nitrogen, sulfur, phosphate and vitamin supplements"
  • PMID:23306120 - SUPPORT (IN_VITRO)
    "fermentation of cellobiose, cellulose and acid-pretreated Populus"
Sulfate with ascorbate reductant sulfate plus ascorbate reductant N/A
  • PMID:23306120 - SUPPORT (IN_VITRO)
    "Both bacteria assimilated sulfate in the presence of ascorbate reductant"
  • PMID:23306120 - SUPPORT (IN_VITRO)
    "increasing the ratio of oxidized to reduced fermentation products"
Mixed cellulose and xylan coculture substrate cellulose and xylan N/A
  • PMID:23306120 - SUPPORT (IN_VITRO)
    "substrates containing cellulose and xylan in defined medium"

Growth Media

Low-ionic-strength defined cellulose/xylan coculture medium

Atmosphere ANAEROBIC

Composition

Component Concentration Unit CHEBI
cellulose N/A N/A CHEBI:18246
xylan N/A N/A CHEBI:37686
sulfate N/A N/A CHEBI:16189

Preparation notes: The PubMed abstract supports a low-ionic-strength defined minimal nutrient medium with decreased nitrogen, sulfur, phosphate, and vitamin supplements; it reports cocultures with cellulose and xylan but does not provide the full salts and vitamin recipe.

Evidence

PMID:23306120

"low ionic strength, defined minimal nutrient medium"

PMID:23306120

"defined medium with sulfate and basal vitamin supplements"