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Clostridium cellulovorans-Beijerinckii AECC ABE Coculture

Community ID CommunityMech:000323
Ecological State ENGINEERED
Environment anaerobic lignocellulose-fed laboratory coculture
ENVO:01001405

A defined anaerobic artificial symbiosis pairing the cellulolytic, butyrate-producing mesophile Clostridium cellulovorans 743B with the non-cellulolytic solventogenic bacterium Clostridium beijerinckii NCIMB 8052. The coculture was designed for consolidated bioprocessing of alkali extracted deshelled corn cobs, where C. cellulovorans saccharifies lignocellulose and produces butyric acid, and C. beijerinckii converts the released soluble sugars and butyric acid into acetone-butanol-ethanol solvents. The system is DOE-relevant as a lignocellulosic biomass-to-biofuel community model using an agricultural residue feedstock.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Clostridium cellulovorans 743B NCBITaxon:573061
PRIMARY_DEGRADER CROSS_FEEDER
ABUNDANT
  • PMID:25023325 - SUPPORT (IN_VITRO)
    "Clostridium cellulovorans 743B to saccharify lignocellulose and produce butyric acid"
  • PMID:25023325 - SUPPORT (IN_VITRO)
    "celluloytic, anaerobic, butyrate-producing mesophile (C. cellulovorans 743B)"
Clostridium beijerinckii NCIMB 8052 NCBITaxon:1520
SECONDARY_FERMENTER CROSS_FEEDER
ABUNDANT
  • PMID:25023325 - SUPPORT (IN_VITRO)
    "Clostridium beijerinckii NCIMB 8052 to produce butanol"
  • PMID:25023325 - SUPPORT (IN_VITRO)
    "non-celluloytic, solventogenic bacterium (C. beijerinckii NCIMB 8052)"

Ecological Interactions

Ecological interaction network for Clostridium cellulovorans-Beijerinckii AECC ABE Coculture Bipartite graph where circle nodes represent taxa and each ecological interaction is drawn as a distinct non-circular symbol, with colour repeating the same distinction (cross-feeding, competition).
Taxon
Cross-feeding
Competition

Lignocellulose Saccharification Feeding Solventogenesis

CROSS_FEEDING

Source Taxon: Clostridium cellulovorans 743B

Target Taxon: Clostridium beijerinckii NCIMB 8052

Metabolites: cellulose (CHEBI:18246), ethanol (CHEBI:16236), butanol (CHEBI:28885), acetone (CHEBI:15347)

Biological Processes:

Evidence

  • PMID:25023325 - SUPPORT (IN_VITRO)
    "soluble sugars and butyric acid generated can be subsequently utilized by Clostridium beijerinckii NCIMB 8052"
  • PMID:25023325 - SUPPORT (IN_VITRO)
    "produce solvents by consolidated bioprocessing (CBP) with alkali extracted deshelled corn cobs (AECC)"

Butyric Acid Support of Butanol Production

CROSS_FEEDING

Source Taxon: Clostridium cellulovorans 743B

Target Taxon: Clostridium beijerinckii NCIMB 8052

Metabolites: butyric acid (CHEBI:30772), butanol (CHEBI:28885)

Biological Processes:

Evidence

  • PMID:25023325 - SUPPORT (IN_VITRO)
    "produce butyric acid, instead of adding cellulase and butyric acid to the medium"
  • PMID:25023325 - SUPPORT (IN_VITRO)
    "soluble sugars and butyric acid generated can be subsequently utilized"

Cooperation and Competition During Coculture Dynamics

COMPETITION

Evidence

  • PMID:25023325 - SUPPORT (IN_VITRO)
    "mechanisms of cooperation and competition between the two strains during the co-culture process"
  • PMID:25023325 - SUPPORT (IN_VITRO)
    "real-time PCR assay based on the 16S rRNA gene sequence was performed"

External Resources

Name Repository Resource ID
Exact-system primary publication - AECC to ABE artificial symbiosis
Primary publication reporting AECC degradation and ABE solvent production by the C. cellulovorans 743B and C. beijerinckii NCIMB 8052 coculture. 		OTHER PMID:25023325
  • PMID:25023325 - SUPPORT (IN_VITRO)
    "co-culture of Clostridium beijerinckii and Clostridium cellulovorans"
Open full text - AECC to ABE artificial symbiosis
Open-access full text for the Microbial Cell Factories study of the C. cellulovorans 743B and C. beijerinckii NCIMB 8052 coculture. 		OTHER PMCID:PMC4223388

Environmental Factors

Factor Value Unit
Alkali extracted deshelled corn cobs sole carbon source N/A
  • PMID:25023325 - SUPPORT (IN_VITRO)
    "alkali extracted deshelled corn cobs (AECC), a low-cost renewable feedstock, as the sole carbon source"
Optimized AECC degradation 68.6 g/L AECC degraded N/A
Solvent production 11.8 g/L total solvents in less than 80 h N/A
  • PMID:25023325 - SUPPORT (IN_VITRO)
    "produced 11.8 g/L solvents"
  • PMID:25023325 - SUPPORT (IN_VITRO)
    "2.64 g/L acetone, 8.30 g/L butanol and 0.87 g/L ethanol"

Growth Media

AECC consolidated bioprocessing coculture

Atmosphere ANAEROBIC

Composition

Component Concentration Unit CHEBI
alkali extracted deshelled corn cobs 68.6 g/L degraded under optimized conditions N/A
butanol N/A N/A CHEBI:28885

Preparation notes: The PubMed abstract supports AECC as the sole carbon source and the anaerobic cellulolytic/solventogenic coculture design; detailed mineral salts and pH-control conditions are not curated from the abstract.

Evidence

PMID:25023325

"alkali extracted deshelled corn cobs (AECC), a low-cost renewable feedstock, as the sole carbon source"

PMID:25023325

"in less than 80 h"