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Cold-Seep ANME-2b and Seep-SRB1g Carbon Monoxide-Oxidizing Syntrophic Consortium

Community ID CommunityMech:000475
Ecological State PERTURBED
Environment marine sediment
ENVO:03000033 · HabitatMech ↗

Syntrophic consortium of anaerobic methane-oxidizing archaea (ANME-2b, family Candidatus Methanocomedenaceae / Candidatus Methanomarinus) and sulfate-reducing bacteria (Seep-SRB1g, phylum Thermodesulfobacteriota) from deep-sea methane seep sediments on the Costa Rica margin capable of oxidizing carbon monoxide (CO) in the absence of methane. CO oxidation is coupled syntrophically to dissimilatory sulfate reduction and, under sulfate-free conditions, to CO2 (bicarbonate) reduction to methane by ANME-2b to support cellular maintenance.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Candidatus Methanocomedenaceae NCBITaxon:3386223 · TaxonMech · TraitMech
PRIMARY_DEGRADER SYNTROPHIC_PARTNER
DOMINANT
  • PMID:41980960 - SUPPORT (IN_VITRO)
    "The oxidation of CO was coupled with sulphate-reduction by syntrophic consortia, and, in the absence of sulphate, through CO2 reduction to methane by ANME-2."
Thermodesulfobacteriota NCBITaxon:200940 · TaxonMech · TraitMech
SYNTROPHIC_PARTNER
ABUNDANT
  • PMID:41980960 - SUPPORT (IN_VITRO)
    "Here, we show that environmental ANME-SRB consortia can oxidize CO in the absence of methane, in anoxic microcosm experiments using a combination of stable isotope geochemical tracers, metatranscriptomics, and single cell activity measurements (FISH-nanoSIMS)."

Ecological Interactions

The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.

Ecological interaction network for Cold-Seep ANME-2b and Seep-SRB1g Carbon Monoxide-Oxidizing Syntrophic Consortium Bipartite graph where circles represent taxa and non-circular symbols represent interactions, with distinct shapes and colours for syntrophy.
Taxon
Syntrophy

Anaerobic Carbon Monoxide Oxidation by ANME-2 in Methane-Depleted Microcosms

SYNTROPHY

In the absence of methane, ANME-2 archaea (Candidatus Methanocomedenaceae) utilize carbon monoxide as a low-potential alternative electron donor, oxidizing CO to bicarbonate/CO2.

Source Taxon: Candidatus Methanocomedenaceae

Target Taxon: Thermodesulfobacteriota

Metabolites: carbon monoxide (CHEBI:17245 · MediaIngredientMech · CMMMech), hydrogencarbonate (CHEBI:17544 · MediaIngredientMech · CMMMech)

Biological Processes:

Downstream Effects:
→ Syntrophic Carbon Monoxide Oxidation Coupled to Sulfate and Bicarbonate Reduction

DIRECTLY IMPLIED: Electrons derived from anaerobic CO oxidation by ANME-2 are transferred to syntrophic sulfate-reducing bacterial partners to reduce sulfate, or used by ANME-2 to reduce CO2 to methane when sulfate is absent.

Evidence

  • PMID:41980960 - SUPPORT (IN_VITRO)
    "Here, we show that environmental ANME-SRB consortia can oxidize CO in the absence of methane, in anoxic microcosm experiments using a combination of stable isotope geochemical tracers, metatranscriptomics, and single cell activity measurements (FISH-nanoSIMS)."
  • PMID:41980960 - SUPPORT (IN_VITRO)
    "The oxidation of CO was coupled with sulphate-reduction by syntrophic consortia, and, in the absence of sulphate, through CO2 reduction to methane by ANME-2."

Syntrophic Carbon Monoxide Oxidation Coupled to Sulfate and Bicarbonate Reduction

SYNTROPHY

In methane-depleted anoxic cold-seep microcosms, ANME-2 archaea (Candidatus Methanocomedenaceae) oxidize carbon monoxide (CO) to bicarbonate/CO2 and transfer reducing equivalents to syntrophic sulfate-reducing bacteria (Thermodesulfobacteriota) to drive dissimilatory sulfate reduction to sulfide; when sulfate is absent, ANME-2 couples CO oxidation to CO2 reduction to methane.

Source Taxon: Candidatus Methanocomedenaceae

Target Taxon: Thermodesulfobacteriota

Metabolites: carbon monoxide (CHEBI:17245 · MediaIngredientMech · CMMMech), sulfate (CHEBI:16189 · MediaIngredientMech · CMMMech), hydrogencarbonate (CHEBI:17544 · MediaIngredientMech · CMMMech), methane (CHEBI:16183 · MediaIngredientMech · CMMMech), hydrogen sulfide (CHEBI:16136 · MediaIngredientMech · CMMMech)

Biological Processes:

Downstream Effects:
→ Carbon Monoxide-Driven Cellular Maintenance Under Energy-Generating Complex Downregulation

DIRECTLY IMPLIED: Syntrophic CO respiration coupled to sulfate reduction provides low-flux metabolic energy that sustains ANME-SRB cellular maintenance while canonical methanotrophic energy-generating complexes (mtr, rnf) are downregulated.

Evidence

  • PMID:41980960 - SUPPORT (IN_VITRO)
    "The oxidation of CO was coupled with sulphate-reduction by syntrophic consortia, and, in the absence of sulphate, through CO2 reduction to methane by ANME-2."
  • PMID:41980960 - SUPPORT (IN_VITRO)
    "Paired single cell FISH-nanoSIMS analysis of anabolic activity indicates that CO respiration appears to support cell maintenance rather than active growth, consistent with the observed down-regulation of energy generating complexes in ANME (e.g., mtr, rnf, etc.)."

Carbon Monoxide-Driven Cellular Maintenance Under Energy-Generating Complex Downregulation

SYNTROPHY

Single-cell FISH-nanoSIMS and metatranscriptomics show that syntrophic CO respiration supports cellular maintenance of ANME-2 and sulfate-reducing bacteria during methane limitation while canonical energy-generating complexes (mtr, rnf) are downregulated.

Source Taxon: Thermodesulfobacteriota

Target Taxon: Candidatus Methanocomedenaceae

Metabolites: carbon monoxide (CHEBI:17245 · MediaIngredientMech · CMMMech), sulfate (CHEBI:16189 · MediaIngredientMech · CMMMech)

Biological Processes:

Evidence

  • PMID:41980960 - SUPPORT (IN_VITRO)
    "Paired single cell FISH-nanoSIMS analysis of anabolic activity indicates that CO respiration appears to support cell maintenance rather than active growth, consistent with the observed down-regulation of energy generating complexes in ANME (e.g., mtr, rnf, etc.)."

Environmental Factors

Factor Value Unit
Carbon Monoxide Partial Pressure and Sulfate Availability 0.1 to 1.0 bar CO headspace; presence or absence of 5 mM sulfate N/A
  • PMID:41980960 - SUPPORT (IN_VITRO)
    "Carbon monoxide (CO) has been reported in seep environments and represents a thermodynamically favourable alternative electron donor due to its low reduction potential."

Cross-Mech Suite Links

Explore related entities across the CultureBotAI X-Mech knowledge bases:

HabitatMech: marine sediment ↗ TaxonMech: Candidatus Methanocomedenaceae ↗ TraitMech: Candidatus Methanocomedenaceae ↗ TaxonMech: Thermodesulfobacteriota ↗ TraitMech: Thermodesulfobacteriota ↗ CultureMech Growth Media ↗ PathwayMech Pathways ↗