A defined two-fungus coculture pairing Cordyceps militaris ZYb8 with Malbranchea ostraviensis SS1 for natural-product discovery. Yang et al. 2026 used LC-MS/MS molecular networking and compound isolation to show that the coculture produced eight previously undescribed aromatic compounds, including eutypoid G-K, gotjawalides A-B, and ocauxarthrol B, along with eleven known natural products; antibacterial assays on the isolated products found weak to moderate selective activity against Gram-positive and Gram-negative bacterial test strains.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
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| Cordyceps militaris ZYb8 — ontology label: Cordyceps militaris | NCBITaxon:73501 · TaxonMech · TraitMech | N/A | |
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| Malbranchea ostraviensis SS1 — ontology label: Malbranchea ostraviensis | NCBITaxon:1220168 · TaxonMech · TraitMech | N/A | |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Coculture-Derived Aromatic Natural Products
The C. militaris ZYb8 and M. ostraviensis SS1 coculture yielded eight previously undescribed aromatic natural products, including eutypoid G-K, gotjawalides A-B, and ocauxarthrol B, plus eleven known compounds.
Participating Taxa: Cordyceps militaris ZYb8, Malbranchea ostraviensis SS1
Evidence
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PMID:42543088 - SUPPORT (IN_VITRO)"eight previously undescribed compounds-eutypoid G-K (1-5), gotjawalides A-B (6-7), and ocauxarthrol B (8)-along with 11 known natural products (9-19)"
Coculture-Derived Antibacterial Products
Compounds isolated from the two-fungus coculture showed weak to moderate selective antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Streptococcus pneumoniae, and Klebsiella pneumoniae.
Participating Taxa: Cordyceps militaris ZYb8, Malbranchea ostraviensis SS1
Evidence
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PMID:42543088 - SUPPORT (IN_VITRO)"Antimicrobial assays revealed that compounds 1-13, 15-16, and 19 exhibited weak to moderate selective antibacterial activities against S. aureus, B. subtilis, S. pneumoniae, and K. pneumoniae"
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Which medium, inoculum ratio, vessel, incubation time, and extraction protocol were used for the C. militaris ZYb8 and M. ostraviensis SS1 coculture that yielded the butenolides, gotjawalides, ocauxarthrol B, and known products?
The PubMed abstract names the two strains, LC-MS/MS molecular networking workflow, isolated coculture products, and antibacterial assays, but Europe PMC did not expose an open-access full text with cultivation, extraction, or isolation details.
Attaches to: engineering_design, ecological_interactions#Coculture-Derived Aromatic Natural Products
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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Primary article - Cordyceps-Malbranchea antimicrobial coculture
Fitoterapia article reporting aromatic butenolides and an anthraquinone derivative isolated from a C. militaris ZYb8 and M. ostraviensis SS1 coculture. |
OTHER | doi:10.1016/j.fitote.2026.107417 |
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