Cross-kingdom synthetic microbial community (13 bacterial and 6 fungal strains) assembled from signature taxa enriched in the low-phosphorus rhizosphere of cucumber (Cucumis sativus): bacterial genera Bacillus, Cupriavidus, Sphingopyxis, and Devosia together with fungal genera Aspergillus and Amesomyces (reported as Amesia). Recruited and sustained by low-phosphorus distinguished rhizosphere metabolites (succinic acid, azelaic acid, threonic acid, and methionine), the cross-kingdom SynCom engages in mutualistic biofilm and hyphal facilitation and reduces Fusarium oxysporum f. sp. cucumerinum abundance by 85-99%, suppressing cucumber Fusarium wilt even under high-phosphorus conditions.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Bacillus — ontology label: Bacillus <firmicutes> | NCBITaxon:1386 · TaxonMech · TraitMech |
PATHOGEN_ANTAGONIST
SYNTROPHIC_PARTNER
|
DOMINANT |
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| Devosia | NCBITaxon:46913 · TaxonMech · TraitMech |
PATHOGEN_ANTAGONIST
SYNTROPHIC_PARTNER
|
ABUNDANT |
|
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| Sphingopyxis | NCBITaxon:165697 · TaxonMech · TraitMech |
PATHOGEN_ANTAGONIST
SYNTROPHIC_PARTNER
|
ABUNDANT |
|
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| Cupriavidus | NCBITaxon:106589 · TaxonMech · TraitMech |
PATHOGEN_ANTAGONIST
SYNTROPHIC_PARTNER
|
ABUNDANT |
|
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| Aspergillus | NCBITaxon:5052 · TaxonMech · TraitMech |
PATHOGEN_ANTAGONIST
SYNTROPHIC_PARTNER
|
ABUNDANT |
|
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| Amesomyces | NCBITaxon:1934349 · TaxonMech · TraitMech |
PATHOGEN_ANTAGONIST
SYNTROPHIC_PARTNER
|
ABUNDANT |
|
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Metabolite-Recruited Cross-Kingdom Pathogen Suppression of Fusarium oxysporum
MUTUALISMLow-phosphorus rhizosphere metabolites (succinic acid, azelaic acid, threonic acid, and methionine) recruit a cross-kingdom synthetic community of bacterial (Bacillus, Devosia, Sphingopyxis, Cupriavidus) and fungal (Aspergillus, Amesomyces) isolates that solubilize phosphate and reduce Fusarium oxysporum f. sp. cucumerinum abundance by 85%.
Participating Taxa: Bacillus, Devosia, Sphingopyxis, Cupriavidus, Aspergillus, Amesomyces
Metabolites: phosphate (CHEBI:26020 · MediaIngredientMech · CMMMech), succinic acid (CHEBI:15741 · MediaIngredientMech · CMMMech), methionine (CHEBI:16811 · MediaIngredientMech · CMMMech)
Biological Processes:
- phosphate-containing compound metabolic process (GO:0006796 · PathwayMech)
- defense response to fungus (GO:0050832 · PathwayMech)
Evidence
-
PMID:41882811 - SUPPORT (IN_VIVO)"To validate the functional role of microbes-metabolites interaction, we constructed a synthetic microbial community composed of the signature taxa from the low phosphorus microbiota, which, when combined with low phosphorus metabolites, reduced pathogen abundance by 85% and conferred disease suppression under high phosphorus conditions."
Cross-Kingdom Devosia and Amesomyces Biofilm and Colony Mutualism
MUTUALISMPairwise co-cultivation of Devosia sp. B2 and Amesomyces (Amesia) sp. F7 mutually promotes bacterial biofilm formation and fungal colony radial expansion.
Source Taxon: Devosia
Target Taxon: Amesomyces
Biological Processes:
- biofilm formation (GO:0042710 · PathwayMech)
DIRECTLY IMPLIED: Mutual promotion of bacterial biofilm formation and fungal colony growth between Devosia and Amesomyces strengthens cross-kingdom SynCom assembly, contributing to 85% reduction of Fusarium oxysporum abundance.
Evidence
-
PMID:41882811 - SUPPORT (IN_VITRO)"(i) Mutual promotion (reflected by concurrent enhancement of bacterial biofilm formation and fungal colony diameter) was observed between specific fungal-bacterial pairs, including: Amesia sp. F7 & Devosia sp. B2; Amesia sp. F7 & Bacillus sp. B10; Aspergillus sp. F5 and Bacillus sp. B18."
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PMID:41882811 - SUPPORT (IN_VIVO)"To validate the functional role of microbes-metabolites interaction, we constructed a synthetic microbial community composed of the signature taxa from the low phosphorus microbiota, which, when combined with low phosphorus metabolites, reduced pathogen abundance by 85% and conferred disease suppression under high phosphorus conditions."
Unidirectional Cross-Kingdom Facilitation of Cupriavidus by Aspergillus
COLONIZATION_FACILITATIONAspergillus isolates (F3 and F5) unidirectionally facilitate the growth and biofilm development of Cupriavidus sp. B12 in cross-kingdom co-culture assays.
Source Taxon: Aspergillus
Target Taxon: Cupriavidus
Biological Processes:
- biofilm formation (GO:0042710 · PathwayMech)
DIRECTLY IMPLIED: Unidirectional growth facilitation of Cupriavidus by Aspergillus supports cross-kingdom SynCom robustness and cooperative suppression of cucumber Fusarium wilt.
Evidence
-
PMID:41882811 - SUPPORT (IN_VITRO)"(ii) Unidirectional facilitation (where one partner enhanced the growth of the other without a reciprocal benefit) characterized other interactions, including Aspergillus sp. F4 & Bacillus sp. B10; Aspergillus sp. F3 & Cupriavidus sp. B12; Aspergillus sp. F5 & Cupriavidus sp. B12)."
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PMID:41882811 - SUPPORT (IN_VIVO)"Amplicon sequencing analyses reveal that low phosphorus conditions promote microbial community stochastic assembly, enriching beneficial genera (bacterial genera Bacillus, Devosia, Sphingopyxis, Cupriavidus, and fungal genera Aspergillus and Amesia) with dual functions of phosphate solubilization and disease resistance, and enhancing cross-kingdom network robustness."
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Soil Phosphorus Availability and Low-P Rhizosphere Metabolites | Low phosphorus (500 uM Pi) vs. high phosphorus (2 mM Pi); 100 uM LPDRMs | N/A |
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