A six-isolate synthetic microbial community assembled from microbial contaminants that originated in the cultivated-meat cell-culture environment. Whole-genome functional mapping suggested potential metabolic complementarity and ecological robustness among the contaminants, and the SynCom was used as a severe cell-cultured-meat contamination model for antimicrobial-peptide intervention tests. In a screen of 11 antimicrobial peptides and proteins, the synthetic peptide 1018-k6 was the most effective agent, with a 37.5 μg/mL minimum inhibitory concentration against the SynCom and complete microbial inhibition across a 14-day production-cycle assay challenged with 10^6 CFU/mL bacteria.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| six cultivated-meat bacterial contaminants — ontology label: Bacteria | NCBITaxon:2 · TaxonMech · TraitMech | N/A | |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Six-Contaminant Potential Metabolic Complementarity
WGS-based functional mapping suggested that the six cell-culture-environment contaminants had potential metabolic complementarity and ecological robustness before they were assembled into a cultivated-meat contamination SynCom; the PubMed abstract does not identify the pairwise topology, exact metabolites, or exact contaminant taxa.
Biological Processes:
- biological process involved in interspecies interaction between organisms (GO:0044419 · PathwayMech)
Evidence
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PMID:42425643 - SUPPORT (COMPUTATIONAL)"WGS-based functional mapping suggested potential metabolic complementarity and ecological robustness among them."
1018-k6 SynCom Antimicrobial Suppression
The synthetic peptide 1018-k6 was selected from an 11-agent antimicrobial-peptide/protein screen because it was the most effective agent against the assembled SynCom, and it completely inhibited the microbial community across a 14-day production-cycle assay under 10^6 CFU/mL bacterial-load challenge conditions.
Biological Processes:
- growth (GO:0040007 · PathwayMech)
Evidence
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PMID:42425643 - SUPPORT (IN_VITRO)"Within 11 tested antimicrobial peptides and proteins, the synthetic peptide 1018-k6 was identified as the most effective antimicrobial agent, showing a minimum inhibitory concentration (MIC) of 37.5 μg/mL against the SynCom."
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PMID:42425643 - SUPPORT (IN_VITRO)"The long-term suppression assay demonstrated that 1018-k6 maintained complete microbial inhibition over a 14-day production cycle, even under exposure to high bacterial loads of 10^6 CFU/mL."
Knowledge gaps & discussions (2)
KNOWLEDGE_GAP OPEN Which six microbial contaminants, exact taxonomic identities, and strain designations made up the cultivated-meat contamination SynCom?
The PubMed abstract reports six microbial contaminants from the cell-culture environment and states that the isolates were assembled into the SynCom, but it does not name the six species or strains. Resolving those identities is required before adding NCBITaxon-grounded taxonomy members.
Attaches to: taxonomy, engineering_design
1 evidence item(s)
In this study, six microbial contaminants originating from the cell culture environment were identified
Supports the existence of six identified contaminants while illustrating that the abstract omits their names.
KNOWLEDGE_GAP OPEN What animal cell type, animal component-free medium composition, vessel format, and dosing regimen were used in the 14-day cultivated-meat production-cycle challenge assay?
The PubMed abstract gives the SynCom MIC, the 1018-k6 long-term inhibition result, the 14-day duration, and the 10^6 CFU/mL bacterial-load challenge, but not the complete cell-culture medium, vessel geometry, cell-line identity, or peptide dosing schedule.
Attaches to: growth_media, cultivation_setup, environmental_factors#Long-term high-load suppression assay
1 evidence item(s)
1018-k6 maintained complete microbial inhibition over a 14-day production cycle, even under exposure to high bacterial loads of 10^6 CFU/mL.
Captures the abstract's quantitative suppression-assay values while leaving the full protocol details open.
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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PubMed record for Lin et al. 2026
PubMed record for the Food Microbiology article describing the six-contaminant cultivated-meat SynCom and 1018-k6 antimicrobial intervention. |
OTHER | PMID:42425643 |
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DOI landing page for the cultivated-meat SynCom article
DOI landing page for the Food Microbiology article describing the six-contaminant cultivated-meat SynCom and 1018-k6 antimicrobial intervention. |
OTHER | doi:10.1016/j.fm.2026.105164 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| 1018-k6 minimum inhibitory concentration | 37.5 μg/mL | N/A |
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| Long-term high-load suppression assay | 14 days; 10^6 CFU/mL bacterial load | N/A |
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| 1018-k6 effective-dose cell compatibility | N/A | |
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