A simplified synthetic nitrifying microbial community pairing Bacillus badius NBRC 15713 with Nitrosomonas europaea NBRC 14298 and Nitrobacter winogradskyi NBRC 14297 for sequential ammonification and nitrification under organic nitrogen conditions. The co-culture achieved complete ammonification and nitrification under selected organic nitrogen conditions, accumulated approximately 56 mg-N/L nitrate-nitrogen with no residual nitrite-nitrogen, and yielded RT-qPCR profiles linking N. europaea and N. winogradskyi abundance changes to inorganic nitrogen concentrations during cultivation.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Bacillus badius NBRC 15713 — ontology label: Pseudobacillus badius | NCBITaxon:1455 · TaxonMech · TraitMech | N/A | |
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| Nitrosomonas europaea NBRC 14298 — ontology label: Nitrosomonas europaea | NCBITaxon:915 · TaxonMech · TraitMech | N/A | |
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| Nitrobacter winogradskyi NBRC 14297 — ontology label: Nitrobacter winogradskyi | NCBITaxon:913 · TaxonMech · TraitMech | N/A | |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Sequential organic-nitrogen conversion
CROSS_FEEDINGThe three defined strains jointly perform complete ammonification and nitrification under selected organic nitrogen conditions, with nitrate accumulation and no residual nitrite detected in the assayed co-culture.
Participating Taxa: Bacillus badius NBRC 15713, Nitrosomonas europaea NBRC 14298, Nitrobacter winogradskyi NBRC 14297
Metabolites: ammonium (CHEBI:28938 · MediaIngredientMech · CMMMech), nitrite (CHEBI:16301 · MediaIngredientMech · CMMMech), nitrate (CHEBI:17632 · MediaIngredientMech · CMMMech)
Biological Processes:
- nitrification (GO:0019329 · PathwayMech)
Evidence
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PMID:42668143 - SUPPORT (IN_VITRO)"A simplified synthetic nitrifying microbial community capable of sequential ammonification and nitrification under organic conditions was constructed using three defined strains: Bacillus badius NBRC 15713, Nitrosomonas europaea NBRC 14298, and Nitrobacter winogradskyi NBRC 14297."
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PMID:42668143 - SUPPORT (IN_VITRO)"This co-culture achieved complete ammonification and nitrification under selected organic nitrogen conditions, with NO₃⁻-N accumulation of approximately 56 mg-N/L and no residual NO₂⁻-N detected."
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Resolve the exact medium formulation, inoculation ratio, temperature, atmosphere, and vessel used for the defined three-strain organic-nitrogen nitrifying community.
The cached PubMed abstract identifies the three NBRC strains, the organic-nitrogen function, nitrate/nitrite readouts, and RT-qPCR abundance tracking, but it does not report the exact organic nitrogen substrates, medium formulation, inoculation ratio, temperature, atmosphere, or vessel. Those cultivation details should be filled from the article Methods when an accessible full text is inspected.
Attaches to: growth_media, cultivation_setup, engineering_design
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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PubMed entry for the defined three-strain nitrifying community
PubMed record for Inzali et al. 2026. |
OTHER | PMID:42668143 |
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DOI for the defined three-strain nitrifying community article
Version-of-record DOI for Inzali et al. 2026. |
OTHER | doi:10.1093/bbb/zbag131 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| selected organic nitrogen conditions | selected organic nitrogen conditions | N/A |
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Cross-Mech Suite Links
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