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Dehalococcoides-Desulfovibrio Lactate-Fed TCE Dechlorination Coculture

Community ID CommunityMech:000199
Ecological State ENGINEERED
Environment anaerobic chlorinated-ethene laboratory coculture
ENVO:01001405

A defined anaerobic syntrophic coculture pairing Dehalococcoides mccartyi strain 195 with Desulfovibrio vulgaris Hildenborough. In this laboratory model for chlorinated-solvent groundwater bioremediation, D. vulgaris Hildenborough ferments lactate and supplies hydrogen and acetate that support strain 195 growth and reductive dechlorination. The system is DOE-relevant because it resolves metabolite exchange and stress responses that affect organohalide-respiring communities used for in situ trichloroethene bioremediation.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Dehalococcoides mccartyi strain 195 NCBITaxon:243164
PRIMARY_DEGRADER SYNTROPHIC_PARTNER
DOMINANT
  • PMID:21881617 - SUPPORT (IN_VITRO)
    "Dehalococcoides ethenogenes strain 195 (DE195)"
  • PMID:28159790 - SUPPORT (IN_VITRO)
    "Dehalococcoides mccartyi 195"
Desulfovibrio vulgaris Hildenborough NCBITaxon:882
SECONDARY_FERMENTER SYNTROPHIC_PARTNER CROSS_FEEDER
COMMON
  • PMID:21881617 - SUPPORT (IN_VITRO)
    "Desulfovibrio vulgaris Hildenborough (DVH) as a co-culture"
  • PMID:28159790 - SUPPORT (IN_VITRO)
    "Desulfovibrio vulgaris Hildenborough"

Ecological Interactions

Ecological interaction network for Dehalococcoides-Desulfovibrio Lactate-Fed TCE Dechlorination Coculture Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

Lactate Fermentation Supports Dehalococcoides Growth

SYNTROPHY

Source Taxon: Desulfovibrio vulgaris Hildenborough

Target Taxon: Dehalococcoides mccartyi strain 195

Metabolites: lactate (CHEBI:24996), dihydrogen (CHEBI:18276), acetate (CHEBI:30089)

Biological Processes:

Evidence

  • PMID:21881617 - SUPPORT (IN_VITRO)
    "using lactate as the sole energy and carbon source"
  • PMID:21881617 - SUPPORT (IN_VITRO)
    "capabilities of DVH to ferment lactate to provide H(2) and acetate for growth"

Enhanced TCE Dechlorination By Syntrophic Support

CROSS_FEEDING

Source Taxon: Desulfovibrio vulgaris Hildenborough

Target Taxon: Dehalococcoides mccartyi strain 195

Metabolites: trichloroethene (CHEBI:16602), chloroethene (CHEBI:28509), ethene (CHEBI:18153), dihydrogen (CHEBI:18276)

Biological Processes:

Evidence

  • PMID:21881617 - SUPPORT (IN_VITRO)
    "maximum dechlorination rates of DE195 were enhanced by approximately three times"
  • PMID:21881617 - SUPPORT (IN_VITRO)
    "Cell yield of DE195 was enhanced in the co-culture"

Sulfate-Reduction Perturbation Of Dechlorination

COMPETITION

Source Taxon: Desulfovibrio vulgaris Hildenborough

Target Taxon: Dehalococcoides mccartyi strain 195

Metabolites: sulfate (CHEBI:16189), hydrogen sulfide (CHEBI:16136), trichloroethene (CHEBI:16602)

Biological Processes:

Evidence

  • PMID:28159790 - SUPPORT (IN_VITRO)
    "high sulfate concentrations (5 mM) inhibited reductive dechlorination due to the toxicity of generated sulfide"
  • PMID:28159790 - SUPPORT (IN_VITRO)
    "sulfide at 5 mM exhibited inhibitory effects on growth of the sulfate reducer"

Associated Datasets

Dataset Type Repository Accession
GSE26815 strain 195 syntrophic coculture transcriptomes
GEO microarray dataset for strain 195 grown in isolation, DvH/195 coculture, and related triculture conditions. 		METATRANSCRIPTOME OTHER GSE26815
PRJNA136009 strain 195 syntrophic growth BioProject
NCBI BioProject linked to the GSE26815 syntrophic growth transcriptomic study. 		OTHER NCBI_BIOPROJECT PRJNA136009

External Resources

Name Repository Resource ID
Exact-system primary publication - DvH/195 sustainable syntrophy
Primary study reporting D. mccartyi strain 195 grown with D. vulgaris Hildenborough as a sustainable syntrophic coculture. 		OTHER PMID:21881617
  • PMID:21881617 - SUPPORT (IN_VITRO)
    "Desulfovibrio vulgaris Hildenborough (DVH) as a co-culture"
Exact-system isotope-fractionation publication - Dhc195/DvH coculture
Follow-up isotope-fractionation study that included the same Dhc195 and DvH syntrophic coculture composition. 		OTHER PMID:24015929
  • PMID:24015929 - SUPPORT (IN_VITRO)
    "Dhc195 with Desulfovibrio vulgaris Hildenborough"
Exact-system corrinoid publication - Dhc195/DvH lactate coculture
Corrinoid-salvaging study that constructed Dhc195/DvH cocultures and related consortia in cobalamin-free or amended media. 		OTHER PMID:24463969
  • PMID:24463969 - SUPPORT (IN_VITRO)
    "cocultures with Desulfovibrio vulgaris Hildenborough (DvH)"
Exact-system perturbation publication - sulfate effects on DvH/195 consortia
Sulfate and sulfide perturbation study including D. mccartyi 195, D. vulgaris Hildenborough, and DvH/195 constructed consortia. 		OTHER PMID:28159790
  • PMID:28159790 - SUPPORT (IN_VITRO)
    "Dehalococcoides mccartyi 195, the sulfate reducer Desulfovibrio vulgaris Hildenborough"

Environmental Factors

Factor Value Unit
Lactate-fed anaerobic condition lactate as sole energy and carbon source N/A
  • PMID:21881617 - SUPPORT (IN_VITRO)
    "using lactate as the sole energy and carbon source"
Trichloroethene electron acceptor TCE-amended dechlorinating culture N/A
  • PMID:24463969 - SUPPORT (IN_VITRO)
    "trichloroethene (TCE)- and lactate-amended medium"
Sulfate and sulfide exposure sulfate up to 5 mM and sulfide at 5 mM N/A
  • PMID:28159790 - SUPPORT (IN_VITRO)
    "Sulfate (up to 5 mM) did not inhibit the growth or metabolism"
  • PMID:28159790 - SUPPORT (IN_VITRO)
    "sulfide at 5 mM exhibited inhibitory effects"

Growth Media

Lactate-fed anaerobic TCE dechlorination medium

Atmosphere ANAEROBIC

Composition

Component Concentration Unit CHEBI
lactate N/A N/A CHEBI:24996
trichloroethene N/A N/A CHEBI:16602

Preparation notes: The curated sources support lactate-fed anaerobic TCE-dechlorinating coculture conditions; this record does not assert the full defined-medium salts, vitamins, or transfer protocol.

Evidence

PMID:21881617

"using lactate as the sole energy and carbon source"

PMID:24463969

"trichloroethene (TCE)- and lactate-amended medium in cocultures with Desulfovibrio vulgaris Hildenborough (DvH)"