A defined anaerobic three-member dechlorinating consortium containing Dehalococcoides mccartyi strain 195, Desulfovibrio vulgaris Hildenborough, and Pelosinus fermentans R7. In cobalamin-free, TCE- and lactate-amended medium supplemented with the lower ligand DMB, DvH supplies hydrogen while PfR7 supplies corrinoids that Dhc195 salvages and remodels into cobalamin. The triculture is relevant to DOE subsurface bioremediation because it identifies separable hydrogen-transfer and corrinoid-transfer mechanisms that support organohalide respiration in chlorinated-solvent dechlorinating communities.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Dehalococcoides mccartyi strain 195 | NCBITaxon:243164 |
PRIMARY_DEGRADER
SYNTROPHIC_PARTNER
|
DOMINANT |
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| Desulfovibrio vulgaris Hildenborough | NCBITaxon:882 |
SECONDARY_FERMENTER
SYNTROPHIC_PARTNER
CROSS_FEEDER
|
COMMON |
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| Pelosinus fermentans R7 | NCBITaxon:1122947 |
SECONDARY_FERMENTER
CROSS_FEEDER
|
COMMON |
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Ecological Interactions
Hydrogen Provision By Desulfovibrio
SYNTROPHYSource Taxon: Desulfovibrio vulgaris Hildenborough
Target Taxon: Dehalococcoides mccartyi strain 195
Metabolites: lactate (CHEBI:24996), dihydrogen (CHEBI:18276), trichloroethene (CHEBI:16602)
Biological Processes:
- lactate catabolic process (GO:0019520)
- anaerobic respiration (GO:0009061)
Evidence
-
PMID:24463969 - SUPPORT (IN_VITRO)"DvH provided hydrogen"
-
PMID:24463969 - SUPPORT (IN_VITRO)"trichloroethene (TCE)- and lactate-amended medium"
Corrinoid Provision By Pelosinus
CROSS_FEEDINGSource Taxon: Pelosinus fermentans R7
Target Taxon: Dehalococcoides mccartyi strain 195
Metabolites: cobalamin (CHEBI:30411), 5,6-dimethylbenzimidazole (CHEBI:15890)
Biological Processes:
- vitamin metabolic process (GO:0006766)
- cobalamin transport (GO:0015889)
Evidence
-
PMID:24463969 - SUPPORT (IN_VITRO)"PfR7 provided corrinoids to Dhc195"
-
PMID:24463969 - SUPPORT (IN_VITRO)"physiological level of 5,6-dimethylbenzimidazole (DMB)"
Dhc195 Corrinoid Salvaging And Remodeling
CROSS_FEEDINGSource Taxon: Pelosinus fermentans R7
Target Taxon: Dehalococcoides mccartyi strain 195
Metabolites: cobalamin (CHEBI:30411), 5,6-dimethylbenzimidazole (CHEBI:15890)
Biological Processes:
- vitamin metabolic process (GO:0006766)
- cobalamin transport (GO:0015889)
Evidence
-
PMID:24463969 - SUPPORT (IN_VITRO)"imported and remodeled the phenolic corrinoids produced by PfR7 into cobalamin"
-
PMID:24463969 - SUPPORT (COMPUTATIONAL)"CbiZ-dependent corrinoid-remodeling pathway and BtuFCD corrinoid ABC transporter genes"
Associated Datasets
| Dataset | Type | Repository | Accession |
|---|---|---|---|
|
GSE45533 Dhc195 corrinoid salvaging transcriptomics
GEO dataset for Dhc195 transcriptomic responses in defined DvH, PfR7, and triculture conditions. |
METATRANSCRIPTOME | OTHER | GSE45533 |
External Resources
| Name | Repository | Resource ID |
|---|---|---|
|
Exact-system primary publication - Dhc195/DvH/PfR7 corrinoid triculture
Primary publication reporting Dhc195, DvH, and PfR7 defined consortia for corrinoid salvaging and remodeling. |
OTHER | PMID:24463969 |
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|
Exact-system GEO transcriptomics dataset - Dhc195/DvH/PfR7 conditions
GEO record for transcriptomic analyses associated with Dhc195 corrinoid salvaging and remodeling in defined consortia. |
OTHER | GSE45533 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Cobalamin-free medium | no exogenous cobalamin | N/A |
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| DMB lower-ligand supplement | physiological level of DMB | N/A |
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| Lactate and TCE amendment | lactate-amended medium with TCE | N/A |
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