A defined two-member chlorinated-ethene bioremediation coculture pairing Dehalococcoides mccartyi strain BAV1 with Methanosarcina barkeri strain Fusaro. D. mccartyi BAV1 is corrinoid auxotrophic and requires a cobalamin-type cofactor for organohalide respiration. M. barkeri Fusaro produces extracellular factor III, which did not by itself support Dehalococcoides reductive dechlorination activity, but addition of 5,6-dimethylbenzimidazole enabled guided biosynthesis of cobalamin that supported Dehalococcoides activity and growth. The system is relevant to DOE chlorinated-solvent groundwater bioremediation because it resolves a cofactor-mediated interaction controlling reductive dechlorination.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Dehalococcoides mccartyi BAV1 | NCBITaxon:216389 |
CROSS_FEEDER
|
N/A |
|
|||
| Methanosarcina barkeri strain Fusaro | NCBITaxon:269797 |
CROSS_FEEDER
|
N/A |
|
|||
Ecological Interactions
DMB-Guided Cobalamin Support
CROSS_FEEDINGSource Taxon: Methanosarcina barkeri strain Fusaro
Target Taxon: Dehalococcoides mccartyi BAV1
Metabolites: cobalamin (CHEBI:30411), 5,6-dimethylbenzimidazole (CHEBI:15890)
Biological Processes:
- cobalamin biosynthetic process (GO:0009236)
- organohalide respiration (GO:0070812)
Evidence
-
PMID:23479750 - SUPPORT (IN_VITRO)"addition of DMB enabled guided biosynthesis and generated a cobalamin"
-
PMID:23479750 - SUPPORT (IN_VITRO)"supported Dehalococcoides' activity and growth"
Factor III Is Insufficient Without DMB
COMMENSALISMSource Taxon: Methanosarcina barkeri strain Fusaro
Target Taxon: Dehalococcoides mccartyi BAV1
Metabolites: cobalamin (CHEBI:30411)
Biological Processes:
- organohalide respiration (GO:0070812)
Evidence
-
PMID:23479750 - SUPPORT (IN_VITRO)"Neither factor III nor the phenolic cobamides supported Dehalococcoides reductive dechlorination activity"
-
PMID:23479750 - SUPPORT (IN_VITRO)"M. barkeri and the Sporomusa sp. cannot fulfil Dehalococcoides' nutritional requirements"
Cobalamin-Dependent Reductive Dechlorination
CROSS_FEEDINGSource Taxon: Dehalococcoides mccartyi BAV1
Target Taxon: Methanosarcina barkeri strain Fusaro
Metabolites: chloroethene (CHEBI:28509), ethene (CHEBI:18153), cobalamin (CHEBI:30411)
Biological Processes:
- organohalide respiration (GO:0070812)
Evidence
-
PMID:23479750 - SUPPORT (IN_VITRO)"require vitamin B12 (CN-Cbl) to conserve energy via organohalide respiration"
-
PMID:23479750 - SUPPORT (IN_VITRO)"incomplete leading to vinyl chloride (VC) accumulation"
External Resources
| Name | Repository | Resource ID |
|---|---|---|
|
Exact-system cobalamin-guidance publication
Primary publication reporting D. mccartyi BAV1, GT, and FL2 cocultures with M. barkeri Fusaro and acetogens to test guided cobalamin biosynthesis for reductive dechlorination. |
OTHER | PMID:23479750 |
|
||
|
Open full text for cobalamin-guidance publication
Open-access full text corresponding to the guided cobalamin biosynthesis study. |
OTHER | PMCID:PMC3638461 |
|
||
|
BAV1 dechlorination phenotype publication
Primary publication describing detoxification of vinyl chloride to ethene by the BAV1 lineage. |
OTHER | PMID:12840758 |
|
||
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| DMB amendment | 10 microM 5,6-dimethylbenzimidazole | N/A |
|
||
| High cobalamin control | 25 micrograms per liter CN-Cbl | N/A |
|
||
| Limiting cobalamin stress | 1 microgram per liter CN-Cbl | N/A |
|
||