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Dehalococcoides-Syntrophomonas TCE Dechlorination Coculture

Community ID CommunityMech:000183
Ecological State STABLE
Environment anaerobic chlorinated-ethene laboratory coculture
ENVO:01001405

A defined two-member anaerobic syntrophic coculture in which Dehalococcoides mccartyi strain 195 reductively dechlorinates trichloroethene while Syntrophomonas wolfei ferments butyrate or crotonate-derived carbon to provide hydrogen and acetate. The system is relevant to chlorinated-solvent groundwater bioremediation because it models interspecies hydrogen transfer and metabolite exchange that support TCE dechlorination by Dehalococcoides-containing communities.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Dehalococcoides mccartyi 195 NCBITaxon:243164
PRIMARY_DEGRADER SYNTROPHIC_PARTNER
N/A
  • PMID:25576615 - SUPPORT (IN_VITRO)
    "Dehalococcoides mccartyi 195 (strain 195)"
Syntrophomonas wolfei NCBITaxon:863
SECONDARY_FERMENTER SYNTROPHIC_PARTNER
N/A
  • PMID:25576615 - SUPPORT (IN_VITRO)
    "Dehalococcoides mccartyi 195 (strain 195) and Syntrophomonas wolfei were grown"

Ecological Interactions

Ecological interaction network for Dehalococcoides-Syntrophomonas TCE Dechlorination Coculture Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

Butyrate Fermentation Supporting TCE Dechlorination

SYNTROPHY

Source Taxon: Syntrophomonas wolfei

Target Taxon: Dehalococcoides mccartyi 195

Metabolites: butyrate (CHEBI:17968), dihydrogen (CHEBI:18276), acetate (CHEBI:30089), trichloroethene (CHEBI:16602)

Biological Processes:

Evidence

  • PMID:25576615 - SUPPORT (IN_VITRO)
    "using butyrate as an electron donor and carbon source"
  • PMID:25576615 - SUPPORT (IN_VITRO)
    "Aqueous H2 concentrations ranged from 24 to 180 nM"

Reductive Dechlorination by Dehalococcoides

CROSS_FEEDING

Source Taxon: Dehalococcoides mccartyi 195

Target Taxon: Syntrophomonas wolfei

Metabolites: trichloroethene (CHEBI:16602), chloroethene (CHEBI:28509), ethene (CHEBI:18153), dihydrogen (CHEBI:18276)

Biological Processes:

Evidence

  • PMID:25576615 - SUPPORT (IN_VITRO)
    "the minimum H2 threshold value for TCE dechlorination by strain 195"
  • PMID:25576615 - SUPPORT (IN_VITRO)
    "trichloroethene (TCE) as an electron acceptor"
  • PMID:24015929 - SUPPORT (IN_VITRO)
    "reductive dechlorination of trichloroethene (TCE)"

Aggregation-Enhanced Hydrogen Flux

COLONIZATION_FACILITATION

Source Taxon: Syntrophomonas wolfei

Target Taxon: Dehalococcoides mccartyi 195

Metabolites: dihydrogen (CHEBI:18276)

Biological Processes:

  • interspecies interaction between organisms (GO:0044419)

Evidence

  • PMID:25576615 - SUPPORT (IN_VITRO)
    "Cell aggregates during syntrophic growth were observed"
  • PMID:25576615 - SUPPORT (IN_VITRO)
    "demonstrated the need for aggregation"

Associated Datasets

Dataset Type Repository Accession
Dehalococcoides mccartyi 195 genome BioProject
NCBI BioProject for the complete genome of D. mccartyi 195, the dechlorinating partner in the coculture. 		GENOME NCBI_BIOPROJECT PRJNA214
Syntrophomonas wolfei Goettingen G311 genome BioProject
NCBI BioProject for S. wolfei subsp. wolfei strain Goettingen G311, the butyrate-fermenting syntrophic partner. 		GENOME NCBI_BIOPROJECT PRJNA13014

External Resources

Name Repository Resource ID
Exact-system primary publication - D. mccartyi 195 and S. wolfei
Primary publication describing efficient metabolic exchange and electron transfer in the D. mccartyi 195 and S. wolfei TCE-dechlorinating coculture. 		OTHER PMID:25576615
  • PMID:25576615 - SUPPORT (IN_VITRO)
    "Dehalococcoides mccartyi 195 (strain 195) and Syntrophomonas wolfei were grown"
Exact-composition isotope-fractionation publication
Publication that measured TCE carbon isotope fractionation for D. mccartyi 195 with S. wolfei and other growth conditions. 		OTHER PMID:24015929
  • PMID:24015929 - SUPPORT (IN_VITRO)
    "Dhc195 with Syntrophomonas wolfei"
Exact-system S. wolfei reverse-electron-transfer publication
Follow-up publication measuring S. wolfei membrane complexes and gene expression in syntrophic growth with D. mccartyi strain 195. 		OTHER doi:10.3389/fmicb.2016.01795

Environmental Factors

Factor Value Unit
TCE electron acceptor trichloroethene N/A
  • PMID:25576615 - SUPPORT (IN_VITRO)
    "trichloroethene (TCE) as an electron acceptor"
Butyrate electron donor and carbon source butyrate N/A
  • PMID:25576615 - SUPPORT (IN_VITRO)
    "butyrate as an electron donor and carbon source"
Low hydrogen concentration 24 to 180 nM
  • PMID:25576615 - SUPPORT (IN_VITRO)
    "Aqueous H2 concentrations ranged from 24 to 180 nM"

Growth Media

TCE and butyrate anaerobic coculture medium

Atmosphere ANAEROBIC

Composition

Component Concentration Unit CHEBI
trichloroethene N/A N/A CHEBI:16602
butyrate N/A N/A CHEBI:17968

Preparation notes: The PubMed abstract supports butyrate as electron donor/carbon source and TCE as electron acceptor. Full-text methods report the exact serum-bottle formulation, but those details are not asserted here because local reference validation only cached the abstract.

Evidence

PMID:25576615

"butyrate as an electron donor and carbon source"

PMID:25576615

"trichloroethene (TCE) as an electron acceptor"