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Delftia acidovorans YP-3 and Klebsiella oxytoca DX-3 Light-Driven Hydrogen-Producing Denitrifying Co-Culture

Community ID CommunityMech:000481
Ecological State ENGINEERED
Environment anaerobic bioreactor
ENVO:00002124 · HabitatMech ↗

Catalyst-free, light-driven defined bacterial co-culture pairing the light-responsive electroactive bio-photosensitizer bacterium Delftia acidovorans YP-3 with hydrogen-producing and nitrate-reducing Klebsiella oxytoca DX-3 for simultaneous wastewater nitrogen removal and biohydrogen recovery. Through direct contact and soluble redox-active mediators that lower electron-transfer resistance and stimulate NADH metabolism, the YP-3/DX-3 co-culture generates 345.52 mL/L H2 in 48 h and achieves 90.25% nitrate removal with minimal nitrite accumulation in synthetic swine wastewater.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Delftia acidovorans NCBITaxon:80866 · TaxonMech · TraitMech
SYNTROPHIC_PARTNER
DOMINANT
  • PMID:42297253 - SUPPORT (IN_VITRO)
    "Here, a catalyst-free, light-driven co-culture system was developed by pairing the light-responsive electroactive bacterium Delftia acidovorans YP-3 with Klebsiella oxytoca DX-3, a strain that can produce hydrogen and reduce nitrate."
Klebsiella oxytoca NCBITaxon:571 · TaxonMech · TraitMech
ELECTRON_ACCEPTOR SECONDARY_FERMENTER SYNTROPHIC_PARTNER
DOMINANT
  • PMID:42297253 - SUPPORT (IN_VITRO)
    "In batch cultures, the YP-3/DX-3 co-culture generated 345.52 mL/L H2 within 48 h and enhanced the removal of nitrate, nitrite, and total nitrogen compared to the DX-3 monoculture."

Ecological Interactions

The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.

Ecological interaction network for Delftia acidovorans YP-3 and Klebsiella oxytoca DX-3 Light-Driven Hydrogen-Producing Denitrifying Co-Culture Bipartite graph where circles represent taxa and non-circular symbols represent interactions, with distinct shapes and colours for syntrophy.
Taxon
Syntrophy

Light-Driven Interspecies Electron Transfer via Direct Contact and Soluble Redox Mediators

SYNTROPHY

Light-responsive electroactive Delftia acidovorans YP-3 and Klebsiella oxytoca DX-3 establish interspecies electron transfer via direct cell contact, extracellular polymeric substances, and soluble redox-active mediators, lowering electron-transfer resistance and enhancing NADH-related redox metabolism.

Source Taxon: Delftia acidovorans

Target Taxon: Klebsiella oxytoca

Metabolites: dihydrogen (CHEBI:18276 · MediaIngredientMech · CMMMech), nitrate (CHEBI:17632 · MediaIngredientMech · CMMMech)

Biological Processes:

Downstream Effects:
→ Synergistic Biohydrogen Production and Nitrate Reduction in Co-Culture

DIRECTLY IMPLIED: Enhanced redox activity and decreased electron-transfer resistance between D. acidovorans YP-3 and K. oxytoca DX-3 drive simultaneous biohydrogen production and nitrate reduction.

Evidence

  • PMID:42297253 - SUPPORT (IN_VITRO)
    "Electrochemical, extracellular polymeric substance, and metabolomic analyses indicated enhanced redox activity, decreased electron-transfer resistance, NADH-related metabolism, and potential interspecies electron transfer via direct contact and soluble redox-active mediators."
  • PMID:42297253 - SUPPORT (IN_VITRO)
    "In batch cultures, the YP-3/DX-3 co-culture generated 345.52 mL/L H2 within 48 h and enhanced the removal of nitrate, nitrite, and total nitrogen compared to the DX-3 monoculture."

Synergistic Biohydrogen Production and Nitrate Reduction in Co-Culture

SYNTROPHY

In batch co-culture, Delftia acidovorans YP-3 and Klebsiella oxytoca DX-3 generate 345.52 mL/L H2 within 48 h while simultaneously accelerating nitrate, nitrite, and total nitrogen removal compared with K. oxytoca DX-3 monoculture.

Source Taxon: Delftia acidovorans

Target Taxon: Klebsiella oxytoca

Metabolites: dihydrogen (CHEBI:18276 · MediaIngredientMech · CMMMech), nitrate (CHEBI:17632 · MediaIngredientMech · CMMMech)

Biological Processes:

Downstream Effects:
→ High-Efficiency Swine Wastewater Denitrification with Minimal Nitrite Accumulation

DIRECTLY IMPLIED: Co-culture bioaugmentation in a synthetic swine wastewater-fed anaerobic sludge reactor enriches electron-transfer and nitrogen-removal groups, achieving 90.25% nitrate removal with minimal nitrite accumulation.

Evidence

  • PMID:42297253 - SUPPORT (IN_VITRO)
    "In batch cultures, the YP-3/DX-3 co-culture generated 345.52 mL/L H2 within 48 h and enhanced the removal of nitrate, nitrite, and total nitrogen compared to the DX-3 monoculture."
  • PMID:42297253 - SUPPORT (IN_VITRO)
    "In a synthetic swine wastewater-fed anaerobic sludge reactor, the co-culture accomplished 90.25 % nitrate removal, minimized nitrite accumulation, and enriched functional groups related to electron transfer, hydrogen production, and nitrogen removal."

High-Efficiency Swine Wastewater Denitrification with Minimal Nitrite Accumulation

SYNTROPHY

In a synthetic swine wastewater-fed anaerobic sludge reactor, the Delftia acidovorans YP-3 and Klebsiella oxytoca DX-3 co-culture achieves 90.25% nitrate removal, minimizes nitrite accumulation, and enriches functional guilds for electron transfer, hydrogen evolution, and denitrification.

Source Taxon: Klebsiella oxytoca

Target Taxon: Delftia acidovorans

Metabolites: dihydrogen (CHEBI:18276 · MediaIngredientMech · CMMMech), nitrate (CHEBI:17632 · MediaIngredientMech · CMMMech)

Biological Processes:

Evidence

  • PMID:42297253 - SUPPORT (IN_VITRO)
    "In a synthetic swine wastewater-fed anaerobic sludge reactor, the co-culture accomplished 90.25 % nitrate removal, minimized nitrite accumulation, and enriched functional groups related to electron transfer, hydrogen production, and nitrogen removal."

Environmental Factors

Factor Value Unit
Light Illumination and Synthetic Swine Wastewater Nitrate Load Light-driven catalyst-free regime; synthetic swine wastewater N/A
  • PMID:42297253 - SUPPORT (IN_VITRO)
    "In a synthetic swine wastewater-fed anaerobic sludge reactor, the co-culture accomplished 90.25 % nitrate removal, minimized nitrite accumulation, and enriched functional groups related to electron transfer, hydrogen production, and nitrogen removal."

Cross-Mech Suite Links

Explore related entities across the CultureBotAI X-Mech knowledge bases:

HabitatMech: anaerobic bioreactor ↗ TaxonMech: Delftia acidovorans ↗ TraitMech: Delftia acidovorans ↗ TaxonMech: Klebsiella oxytoca ↗ TraitMech: Klebsiella oxytoca ↗ CultureMech Growth Media ↗ PathwayMech Pathways ↗