A defined five-species Drosophila melanogaster gut microbiota assembled from fly-gut isolates of Acetobacter pomorum, Acetobacter tropicalis, Lactobacillus brevis, Lactobacillus fructivorans, and Lactobacillus plantarum. The community was used to colonize axenic Drosophila and test how Acetobacter-Lactobacillus interactions shape host development, glucose, triglyceride allocation, and host-genotype-dependent microbiota effects.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Drosophila gut Acetobacter isolates — ontology label: Acetobacter | NCBITaxon:434 · TaxonMech · TraitMech |
CROSS_FEEDER
SECONDARY_FERMENTER
|
N/A |
|
|||
| Drosophila gut Lactobacillus isolates — ontology label: Lactobacillus | NCBITaxon:1578 · TaxonMech · TraitMech |
CROSS_FEEDER
SECONDARY_FERMENTER
|
N/A |
|
|||
| Drosophila melanogaster | NCBITaxon:7227 · TaxonMech · TraitMech | N/A | |
|
|||
Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Acetobacter-Lactobacillus Nutrient Allocation Interaction
Combined Acetobacter-Lactobacillus treatments alter whole-host triglyceride levels. This joint outcome does not establish metabolite exchange; individual pair responses differ.
Participating Taxa: Drosophila gut Acetobacter isolates, Drosophila gut Lactobacillus isolates, Drosophila melanogaster
Biological Processes:
- lipid metabolic process (GO:0006629 · PathwayMech)
- biological process involved in interspecies interaction between organisms (GO:0044419 · PathwayMech)
Evidence
-
PMID:24242251 - SUPPORT (IN_VIVO)"Only flies with both Acetobacter and Lactobacillus had triglyceride contents restored to the level in conventional flies."
Lactobacillus Promotion of Acetobacter Abundance
Whole-fly Acetobacter CFU increased with L. brevis or L. fructivorans, but not significantly with L. plantarum. CFU endpoints do not establish stable gut residence or growth rates. Grouped canonical taxa must not imply a universal genus-level effect.
Source Taxon: Drosophila gut Lactobacillus isolates
Target Taxon: Drosophila gut Acetobacter isolates
Biological Processes:
- biological process involved in interspecies interaction between organisms (GO:0044419 · PathwayMech)
PARTIAL - greater Acetobacter abundance could contribute to lower host triglycerides. Inoculum perturbations support this model but do not selectively establish abundance as the mediator.
Evidence
-
PMID:24242251 - SUPPORT (IN_VIVO)"Lactobacillus-mediated promotion of Acetobacter abundance in the fly"
Microbiota-Dependent Host Nutrition
Host nutrition depends on microbiota and experimental context. The 2014 single/five-species comparison found no significant weight, protein or glycogen response. The separate 2015 host-genotype study found variation in response magnitude and direction, not universal benefit.
Participating Taxa: Drosophila gut Acetobacter isolates, Drosophila gut Lactobacillus isolates, Drosophila melanogaster
Biological Processes:
- growth (GO:0040007 · PathwayMech)
- glucose metabolic process (GO:0006006 · PathwayMech)
Evidence
-
PMID:24242251 - SUPPORT (IN_VIVO)"The low glucose content of conventional flies was recapitulated in gnotobiotic Drosophila flies colonized with any of the 5 bacterial taxa tested."
-
PMID:25692519 - SUPPORT (IN_VIVO)"effects of eliminating the gut microbiota on five host nutritional indices"
Associated Datasets
| Dataset | Type | Repository | Accession |
|---|---|---|---|
|
Exact-composition publication - Drosophila five-species gnotobiotic microbiota
Primary publication defining the five-species Drosophila gnotobiotic community and measuring development, glucose, triglycerides, and Acetobacter-Lactobacillus interactions. |
PHENOTYPE | OTHER | PMID:24242251 |
|
Exact-composition publication - host genotype and microbiota-dependent nutrition
Follow-up genome-wide association study using the same five-species bacterial suspension to measure host-genotype-dependent nutritional responses. |
PHENOTYPE | OTHER | PMID:25692519 |
|
Source community publication - low-diversity Drosophila gut microbiota
Amplicon survey identifying the five low-diversity Drosophila gut taxa that motivated the defined gnotobiotic community. |
AMPLICON_16S | OTHER | PMID:21631690 |
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Which species-specific interactions and host pathways mediate the nutritional outcomes?
The endpoint-to-panel arrow is redundant, not demonstrated causation. Species-level expansion remains pending under #1408 and canonical roster/role review under #1407. Existing non-graph evidence debt is tracked by #765. No removed publisher body is restored.
Attaches to: ecological_interactions#Acetobacter-Lactobacillus Nutrient Allocation Interaction, ecological_interactions#Lactobacillus Promotion of Acetobacter Abundance, ecological_interactions#Microbiota-Dependent Host Nutrition
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Drosophila yeast-glucose diet | Yeast-glucose agar diet at 25 C with a 12-hour light/dark cycle | N/A |
| five-species inoculum density | 5 x 10^6 total bacterial cells per vial, equal parts for multispecies inocula | N/A |
Growth Media
Cross-Mech Suite Links
Explore related entities across the CultureBotAI X-Mech knowledge bases: