← Back to Communities

Drosophila Five-Species Gnotobiotic Gut Microbiota

Community ID CommunityMech:000160
Ecological State ENGINEERED
Environment Drosophila melanogaster gut
UBERON:0000160

A defined five-species Drosophila melanogaster gut microbiota assembled from fly-gut isolates of Acetobacter pomorum, Acetobacter tropicalis, Lactobacillus brevis, Lactobacillus fructivorans, and Lactobacillus plantarum. The community was used to colonize axenic Drosophila and test how Acetobacter-Lactobacillus interactions shape host development, glucose, triglyceride allocation, and host-genotype-dependent microbiota effects.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Drosophila gut Acetobacter isolates NCBITaxon:434
CROSS_FEEDER SECONDARY_FERMENTER
N/A
  • PMID:25692519 - SUPPORT (IN_VIVO)
    "Acetobacter pomorum DmelCS_004, A. tropicalis DmelCS_006"
  • PMID:21631690 - SUPPORT (COMPUTATIONAL)
    "could be assigned to Acetobacter pomorum, A. tropicalis"
Drosophila gut Lactobacillus isolates NCBITaxon:1578
CROSS_FEEDER SECONDARY_FERMENTER
N/A
  • PMID:25692519 - SUPPORT (IN_VIVO)
    "Lactobacillus brevis DmelCS_003, L. fructivorans DmelCS_002 and L. plantarum DmelCS_001"
  • PMID:21631690 - SUPPORT (COMPUTATIONAL)
    "Lactobacillus brevis, L. fructivorans and L. plantarum"
Drosophila melanogaster NCBITaxon:7227 N/A
  • PMID:24242251 - SUPPORT (IN_VIVO)
    "Using the Drosophila melanogaster gut microbiota"

Ecological Interactions

Ecological interaction network for Drosophila Five-Species Gnotobiotic Gut Microbiota Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

Acetobacter-Lactobacillus Nutrient Allocation Interaction

CROSS_FEEDING

Source Taxon: Drosophila gut Lactobacillus isolates

Target Taxon: Drosophila gut Acetobacter isolates

Biological Processes:

  • lipid metabolic process (GO:0006629)
  • biological process involved in interspecies interaction between organisms (GO:0044419)

Evidence

  • PMID:24242251 - SUPPORT (IN_VIVO)
    "Only flies with both Acetobacter and Lactobacillus had triglyceride contents restored to the level in conventional flies."

Lactobacillus Promotion of Acetobacter Abundance

COLONIZATION_FACILITATION

Source Taxon: Drosophila gut Lactobacillus isolates

Target Taxon: Drosophila gut Acetobacter isolates

Biological Processes:

  • growth (GO:0040007)
  • biological process involved in interspecies interaction between organisms (GO:0044419)

Evidence

  • PMID:24242251 - SUPPORT (IN_VIVO)
    "Lactobacillus-mediated promotion of Acetobacter abundance in the fly"

Microbiota-Dependent Host Nutrition

MUTUALISM

Source Taxon: Drosophila five-species bacterial microbiota

Target Taxon: Drosophila melanogaster

Biological Processes:

Evidence

  • PMID:24242251 - SUPPORT (IN_VIVO)
    "The low glucose content of conventional flies was recapitulated in gnotobiotic Drosophila flies colonized with any of the 5 bacterial taxa tested."
  • PMID:25692519 - SUPPORT (IN_VIVO)
    "Gnotobiotic Drosophila associated with a defined five-species microbiota"

Associated Datasets

Dataset Type Repository Accession
Exact-composition publication - Drosophila five-species gnotobiotic microbiota
Primary publication defining the five-species Drosophila gnotobiotic community and measuring development, glucose, triglycerides, and Acetobacter-Lactobacillus interactions. 		PHENOTYPE OTHER PMID:24242251
Exact-composition publication - host genotype and microbiota-dependent nutrition
Follow-up genome-wide association study using the same five-species bacterial suspension to measure host-genotype-dependent nutritional responses. 		PHENOTYPE OTHER PMID:25692519
Source community publication - low-diversity Drosophila gut microbiota
Amplicon survey identifying the five low-diversity Drosophila gut taxa that motivated the defined gnotobiotic community. 		AMPLICON_16S OTHER PMID:21631690

Environmental Factors

Factor Value Unit
Drosophila yeast-glucose diet Yeast-glucose agar diet at 25 C with a 12-hour light/dark cycle N/A
  • PMID:24242251 - SUPPORT (IN_VIVO)
    "Wolbachia-free Drosophila melanogaster Canton S flies were reared at 25"
five-species inoculum density 5 x 10^6 total bacterial cells per vial, equal parts for multispecies inocula N/A
  • PMID:24242251 - SUPPORT (IN_VIVO)
    "For microbiota of >1 species, each component was added in equal parts to make up the total inoculum"

Growth Media

modified MRS for bacterial maintenance

Temperature 30 C
Atmosphere FACULTATIVE_ANAEROBIC

Preparation notes: Acetobacter members were maintained aerobically, while Lactobacillus plates were kept under carbon dioxide-enriched conditions.

Evidence

PMID:24242251

"All bacteria were maintained on MMRS medium at 30"