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Shewanella-Acetogen Electrosynthetic Consortia for CO2-to-Acetate

Community ID CommunityMech:000298
Ecological State ENGINEERED
Environment microbial electrosynthesis reactor (CO2-to-acetate bioreactor)
ENVO:00002123

Defined synthetic microbial electrosynthesis (MES) consortia constructed by pairing the model bidirectional electroactive bacterium Shewanella oneidensis MR-1 with either the electroactive acetogen Clostridium aceticum or the nonelectroactive acetogen Acetobacterium woodii, mimicking functional guilds of natural MES communities. Co-cultivation boosts CO2-to-acetate production by up to 88%. The two pairings use distinct interspecies electron transfer (IET) modes, namely direct interspecies electron transfer (DIET) via cytochrome c and riboflavin at the biofilm-electrode interface in the S. oneidensis-C. aceticum consortium, versus H2/formate-mediated IET in the planktonic phase for the S. oneidensis-A. woodii consortium. This metabolic stratification lets S. oneidensis act as an "ecosystem engineer" orchestrating electron flow across biofilm and suspension niches.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Shewanella oneidensis MR-1 NCBITaxon:70863
ELECTROGEN ELECTRON_DONOR SYNTROPHIC_PARTNER
N/A
  • PMID:41236292 - SUPPORT (IN_VITRO)
    "defined synthetic consortia are constructed by pairing Shewanella oneidensis MR-1, a model bidirectional electroactive bacterium"
Clostridium aceticum (electroactive acetogen) NCBITaxon:84022
ELECTROTROPH ELECTRON_ACCEPTOR SYNTROPHIC_PARTNER
N/A
  • PMID:41236292 - SUPPORT (IN_VITRO)
    "with Clostridium aceticum (electroactive acetogen) or Acetobacterium woodii (nonelectroactive acetogen)"
Acetobacterium woodii (nonelectroactive acetogen) NCBITaxon:33952
ELECTRON_ACCEPTOR SYNTROPHIC_PARTNER
N/A
  • PMID:41236292 - SUPPORT (IN_VITRO)
    "with Clostridium aceticum (electroactive acetogen) or Acetobacterium woodii (nonelectroactive acetogen)"

Ecological Interactions

Ecological interaction network for Shewanella-Acetogen Electrosynthetic Consortia for CO2-to-Acetate Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

DIET from S. oneidensis to C. aceticum

SYNTROPHY

Source Taxon: Shewanella oneidensis MR-1

Target Taxon: Clostridium aceticum (electroactive acetogen)

Metabolites: riboflavin (CHEBI:17015)

Evidence

  • PMID:41236292 - SUPPORT (IN_VITRO)
    "DIET via cytochrome c and riboflavin dominates at the biofilm-electrode interface in S. oneidensis-C. aceticum consortia"

H2/formate-mediated IET from S. oneidensis to A. woodii

SYNTROPHY

Source Taxon: Shewanella oneidensis MR-1

Target Taxon: Acetobacterium woodii (nonelectroactive acetogen)

Metabolites: dihydrogen (CHEBI:18276), formate (CHEBI:15740)

Evidence

  • PMID:41236292 - SUPPORT (IN_VITRO)
    "whereas S. oneidensis-A. woodii consortia prefer H2/formate-mediated IET in the planktonic phase"

Growth Media

DSMZ-Medium 135 base medium (optimized, cathode chamber)

Temperature 37 °C
Atmosphere ANAEROBIC

Preparation notes: Cathode chamber filled with the culture medium; operated in sequential 7-day cycles with fresh medium added after each cycle.

Evidence

PMID:41236292

"The reaction was conducted in a thermostatic chamber (37 °C) with a headspace atmosphere of N2/CO2 (80:20)"

PMID:41236292

"The culture medium for these bacteria is an optimized DSMZ-Medium135 base medium"