Four-strain core endophytic synthetic bacterial community (SynCom-NS)—composed of Pseudomonas izuensis SaPI1, Leifsonia shinshuensis SaLS1, Brucella anthropi (Ochrobactrum anthropi) SaOA1, and Novosphingobium lindaniclasticum SaNL1 originally isolated from the hyperaccumulator Sedum alfredii—applied to the high-biomass crop Brassica juncea under cadmium stress. SynCom-NS increases soil Cd bioavailability, upregulates root HMA4 metal transporter expression and glutathione-mediated antioxidant defenses, increases plant fresh weight by 82%, and amplifies shoot Cd accumulation 4.07-fold.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Pseudomonas izuensis | NCBITaxon:2684212 · TaxonMech · TraitMech |
SYNTROPHIC_PARTNER
CROSS_FEEDER
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DOMINANT |
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| Leifsonia shinshuensis | NCBITaxon:150026 · TaxonMech · TraitMech |
SYNTROPHIC_PARTNER
CROSS_FEEDER
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DOMINANT |
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| Brucella anthropi | NCBITaxon:529 · TaxonMech · TraitMech |
SYNTROPHIC_PARTNER
CROSS_FEEDER
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DOMINANT |
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| Novosphingobium lindaniclasticum | NCBITaxon:1329895 · TaxonMech · TraitMech |
SYNTROPHIC_PARTNER
CROSS_FEEDER
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DOMINANT |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Microbiome-Driven Glutathione Surge and Phenylpropanoid Cell Wall Reinforcement
MUTUALISMRoot-zone inoculation of Brassica juncea with the four-strain endophytic SynCom-NS induces systemic host defense reprogramming, marked by a >3-fold increase in glutathione levels and activation of phenylpropanoid biosynthesis for cell wall reinforcement.
Participating Taxa: Pseudomonas izuensis, Leifsonia shinshuensis, Brucella anthropi, Novosphingobium lindaniclasticum
Metabolites: cadmium(2+) (CHEBI:48775 · MediaIngredientMech · CMMMech), glutathione (CHEBI:16856 · MediaIngredientMech · CMMMech), hydrogen peroxide (CHEBI:16240 · MediaIngredientMech · CMMMech)
Biological Processes:
- response to oxidative stress (GO:0006979 · PathwayMech)
- defense response (GO:0006952 · PathwayMech)
DIRECTLY IMPLIED: Systemic antioxidant protection and cell wall reinforcement enable tissue-specific HMA4 transporter regulation that accelerates root-to-shoot Cd translocation without phytotoxic collapse.
Evidence
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PMID:42043130 - SUPPORT (IN_VIVO)"Multi-omics analysis demonstrated a systemic modulation of the host's defense machinery, marked by a >3-fold surge in glutathione (GSH) levels and the induction of phenylpropanoid biosynthesis for cell wall reinforcement."
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PMID:42043130 - SUPPORT (IN_VIVO)"SynCom-NS application also mediated tissue-specific regulation of the key metal transporter HMA4, upregulating its expression in roots to accelerate long-distance translocation while downregulating it in shoots."
Synergistic Endophytic HMA4 Regulation and Root-to-Shoot Cadmium Translocation
SYNTROPHYThe four-strain endophytic consortium (Pseudomonas izuensis, Leifsonia shinshuensis, Brucella anthropi, and Novosphingobium lindaniclasticum) coordinates tissue-specific expression of the heavy metal ATPase HMA4, upregulating root HMA4 to accelerate long-distance Cd translocation while downregulating shoot HMA4.
Participating Taxa: Pseudomonas izuensis, Leifsonia shinshuensis, Brucella anthropi, Novosphingobium lindaniclasticum
Metabolites: cadmium(2+) (CHEBI:48775 · MediaIngredientMech · CMMMech)
Biological Processes:
- defense response (GO:0006952 · PathwayMech)
DIRECTLY IMPLIED: Coordinated GSH detoxification and root HMA4 upregulation increase Brassica juncea fresh weight by 82% and shoot Cd accumulation by 4.07-fold, bypassing the growth-dilution trade-off.
Evidence
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PMID:42043130 - SUPPORT (IN_VIVO)"SynCom-NS application also mediated tissue-specific regulation of the key metal transporter HMA4, upregulating its expression in roots to accelerate long-distance translocation while downregulating it in shoots."
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PMID:42043130 - SUPPORT (IN_VIVO)"Crucially, the consortium bypassed the "growth-dilution" trade-off, facilitating a 4.07-fold increase in shoot Cd accumulation."
High-Biomass Cadmium Phytoextraction Bypassing the Growth-Dilution Trade-Off
MUTUALISMSynCom-NS inoculation alleviates cadmium toxicity in Brassica juncea, increasing plant fresh weight by 82%, chlorophyll content by 33%, and shoot Cd accumulation by 4.07-fold.
Participating Taxa: Pseudomonas izuensis, Leifsonia shinshuensis, Brucella anthropi, Novosphingobium lindaniclasticum
Metabolites: cadmium(2+) (CHEBI:48775 · MediaIngredientMech · CMMMech)
Biological Processes:
- response to oxidative stress (GO:0006979 · PathwayMech)
Evidence
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PMID:42043130 - SUPPORT (IN_VIVO)"Pot experiments revealed that SynCom-NS root-zone application significantly alleviated Cd toxicity, increasing total fresh weight by 82% and chlorophyll content by 33%. Crucially, the consortium bypassed the "growth-dilution" trade-off, facilitating a 4.07-fold increase in shoot Cd accumulation."
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Soil Cadmium Contamination | ~1.0 mg/kg soil Cd | N/A |
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