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GLBRC Exometabolite Transwell SynCom System

Community ID CommunityMech:000091
Ecological State ENGINEERED
Environment laboratory bioreactor
ENVO:01001405

A synthetic community assay system for probing chemically mediated microbial interactions through shared exometabolites.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
exometabolite-interacting bacteria NCBITaxon:2
CROSS_FEEDER
N/A
  • PMID:29152587 - SUPPORT (IN_VITRO)
    "we describe a straightforward synthetic community system that can be used to interrogate exometabolite interactions among microorganisms."

Ecological Interactions

Ecological interaction network for GLBRC Exometabolite Transwell SynCom System Bipartite graph where circle nodes represent taxa and each ecological interaction is drawn as a distinct non-circular symbol, with colour repeating the same distinction (cross-feeding).
Taxon
Cross-feeding

Exometabolite-Mediated Interaction

CROSS_FEEDING

Evidence

  • PMID:29152587 - SUPPORT (IN_VITRO)
    "we describe a straightforward synthetic community system that can be used to interrogate exometabolite interactions among microorganisms."

Environmental Factors

Factor Value Unit
defined synthetic community design Synthetic community members are cultured in a filter plate system that separates cells while allowing chemical exchange through shared medium. N/A
  • PMID:29152587 - SUPPORT (IN_VITRO)
    "we describe a straightforward synthetic community system that can be used to interrogate exometabolite interactions among microorganisms."

Growth Media

tryptic soy agar/broth (TSA50) - representative Transwell assay

Temperature 29 °C

Preparation notes: Physically separated populations share a common medium reservoir; plates incubated with gentle shaking to homogenize member access to media and exometabolites.

Evidence

PMID:29152587

"cultures in test tubes were incubated at 29°C at 200 rpm"

PMID:29152587

"each well has a 0.22-μm-pore filter bottom and the plate fits into a shared medium reservoir"